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Application

Note: 51854
Analysis of Acetaminophen with the
Evolution Array UV-Visible Spectrophotometer
Nicole Kreuziger Keppy, Gordon Bain, Ph.D., Michael W. Allen, Ph.D., Thermo Fisher Scientific, Madison, WI, USA

Introduction
Key Words A UV-Visible spectrophotometer is an essential tool for any
Acetaminophen quality control laboratory. It is useful for identity, purity
and quantitative analysis assays of raw materials, active
Equation Calculator ingredients and final products in the manufacturing of:
Paracetamol Pharmaceuticals
Percent Assay Cosmetics
Food and beverages
UV-Visible
Specialty chemicals
Spectroscopy
The wavelength and peaks/valleys functions in the
United States
wavelength monitoring mode of the Thermo Scientific
Pharmacopeia
VISIONcollect software allow the user to identify sample
(USP)
components. Absorbance and ratio functions provide
confirmation of the purity and quantity of these components.
In this application note, the quantity and percent assay of
acetaminophen (also known as Paracetamol) is determined
with a Thermo Scientific Evolution Array UV-Visible
spectrophotometer according to USP methods.

Figure 1: Chemical structure of acetaminophen, C8H9NO2

Experimental
A concentrated stock solution of acetaminophen was
prepared by dissolving approximately 20 mg of dried
acetaminophen standard in 2 mL of methyl alcohol.
This solution was brought to a total volume of 100 mL Figure 2: Experimental Parameters
with water. A working standard solution was prepared
by removing 3 mL of the stock solution and bringing this
solution to a total volume of 100 mL with water. A test
sample of acetaminophen was prepared in the same
fashion as the standard. Water was used as the blank.
The absorbance of the working standard and test
sample was measured at 244 nm using the parameters
indicated in Figure 2. The full-spectrum result for
acetaminophen is shown in Figure 3.
Conclusion In addition to these
offices, Thermo Fisher
Assays for pharmaceuticals can easily be performed using
Scientific maintains
the Evolution Array UV-Visible spectrophotometer and
a network of represen-
VISIONcollect software. According to the USP 29 assay
tative organizations
for acetaminophen, the test sample must contain not less
throughout the world.
than 98.0 percent and not more than 101.0 percent of
C8H9NO2, calculated on the anhydrous basis.1 In this
experiment, the percent assay of acetaminophen in the
test sample was determined experimentally to be 98.5%.
Thus, this acetaminophen test sample was found to be in
agreement with the USP assay test for acetaminophen. Africa-Other
+27 11 570 1840
References Australia
+61 2 8844 9500
1. United States Pharmacopeia and National Formulary (USP 29 NF 24).
Austria
Supplement No. 2. Rockville, MD: United States Pharmacopeia +43 1 333 50 34 0
Figure 3: Spectra of acetaminophen standard and test sample Convention; 2006: 3711.
Belgium
+32 2 482 30 30
Canada
The values for the quantity and percent assay of +1 800 530 8447
acetaminophen were calculated using the following China
equations. +86 10 8419 3588
Denmark
Quantity of acetaminophen in the test sample: +45 70 23 62 60
Europe-Other
A244 (test sample) +43 1 333 50 34 0
= standard sample weight (mg) X
A244 (standard) Finland / Norway /
0.0549 Sweden
= 20.1 mg X +46 8 556 468 00
0.0558
France
= 19.8 mg +33 1 60 92 48 00
Germany
+49 6103 408 1014
Percent assay of acetaminophen in the test sample:
India
Calculated Qty in test sample +91 22 6742 9434
= 100 X Italy
Weight of test sample
+39 02 950 591
19.8 Japan
= 100 X +81 45 453 9100
20.0
Latin America
= 98.5% +1 608 276 5659
Middle East
+43 1 333 50 34 0
The quantity of acetaminophen in the test sample was Netherlands
experimentally determined to be 19.8 mg. The percent +31 76 579 55 55
assay was determined to be 98.5%. South Africa
+27 11 570 1840
Spain
+34 914 845 965
Switzerland
+41 61 716 77 00
UK
+44 1442 233555
USA
+1 800 532 4752

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