BF Biosciences Limited Doc. No.

: Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
1 of 6

DESTRUCTION OF CULTURE MEDIA

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

COVER SHEET

Issue Issue Date Originator Amendments/Justification

00 27-06-2009 Muhammad Adnan Khan New Procedure

_______________________

Valid from : 30-06-2009

Purpose : To establish a procedure for destruction of culture media either

containing microbial growth OR not.

Responsible for Implementation : Manager Quality Operations

Manager Quality Control/Microbiology
A.M. Microbiology
Microbiologist

Total pages : 08

Issued to : 1. Manager Quality Operations

2. Quality Control Manager/Microbiology
3. AM Microbiology
4. Quality Assurance Manager

Reference : WHO
USP
BP/EP
District Laboratory Manual, Vol. 2, Monica Cheesborough

Microbiology
BF/SOP/Mic
 BF Biosciences Limited Doc. No. : Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
2 of 6

DESTRUCTION OF CULTURE MEDIA

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

LIST OF CONTENTS

1. Objective

2. Scope

3. Responsibilities

4. Requirements

5. Procedure

6. Precautions

7. Training

8. Distribution

9. Related Document

10. History

Microbiology
BF/SOP/Mic
 BF Biosciences Limited Doc. No. : Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
3 of 6

DESTRUCTION OF CULTURE MEDIA

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

1. OBJECTIVE

Objective of this SOP is to define a procedure of destruction for cultural media which is used to
test pharmaceutical products like raw materials, bulk, intermediate, finished and also water
(purified water as well as drinking water) for microbiological evaluation.

2. SCOPE

This SOP describes the execution of destruction of culture media in microbiological lab where
cultural media are used for the culturing and monitoring of sterile area, testing of raw materials
and water.

3. RESPONSIBILITIES

3.1. Microbiologist:

- To interface this SOP to the actual practices of flow patterns.

- To ensure that the SOP is updated & reflects the actual execution.

- To review the SOP in case of any change or whenever it is due for revision.

3.2. AM Microbiology:

- To follow the instructions laid down by this SOP.

- To keep the relevant SOPs valid and updated.

3.3. Manager Quality Operations:

- To provide adequate resources for the implementation of this SOP as define.

Microbiology
BF/SOP/Mic
 BF Biosciences Limited Doc. No. : Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
4 of 6

DESTRUCTION OF CULTURE MEDIA

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

4. REQUIREMENTS
4.1. Face mask
4.2. Sterile latex gloves
4.3. Filtered 70% Isopropyl Alcohol spray bottle
4.4. Dettol
4.5. 37% Formalin
4.6. Autoclave
4.7. Spatula
4.8. SS container / glass beaker
4.9. Contaminate petri dishes / tubes / pipettes / flasks
4.10. Liquid detergent
4.11. Heat resistant gloves

5. TESTING PROCEDURE
5.1. After all testing been done, dispose-off all type of media that are used for
microbiological testing.

5.2. Media dishes / flasks / tubes which are not contaminated / they have not any
appearance of microbes, are directly emptied into polybags and add
20 25 mL 37% formalin OR 5% dettol, then liquid medium dispose-off while hot with
running tape water, and then wash the dishes / tubes / flasks.

5.3. If any media dish / flask / tube containing either type of microbial growth like
bacteria / fungi (mold OR yeast) then empty the dish / flask / tube with the help of
spatula into the SS container / glass beaker, firmly seal and place in to the autoclave
chamber.

5.4. Autoclave (SOP #. ) the SS container / beaker containing contaminated

medium which is to be discarded as per validated cycle.

5.5. After autoclaving, add 20 25 mL 37% formalin OR 5% dettol and then dispose-off
liquid medium while hot with running tape water.
Microbiology
BF/SOP/Mic
 BF Biosciences Limited Doc. No. : Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
5 of 6

DESTRUCTION OF CULTURE MEDIA

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

5.6. Keep the empty petri dishes / flasks / tubes for 1 hour in 5% dettol solution OR in
liquid detergent solution, then wash them with tap water and for final rinsing rinse
them with purified water.

6. PRECAUTIONS
6.1 Do not add 70% isopropyl alcohol to media that is to be autoclaved.
6.2. Do not touch surface of autoclave while it is in operation condition.
6.3. Always use heat resistant gloves while dealing with hot chamber and materials.
6.4. Always use sterile latex gloves and face mask.
6.5. Always add disinfectant solution after completion of autoclave process.

7. TRAINING
Training about the contents of this SOP must be imparted to all the concerned personnel once in
a year and also to new employees before starting with the job and the records documented
according to training SOP.

8. DISTRIBUTION
Issuance of New Copy Retrieval of Old version

Department /
Signature Copy # Retrieved from Retrieved by
Person

Original Copy

Copy # 1

Copy # 2

Copy # 3

Copy # 4

9. RELATED DOCUMENT

Microbiology
BF/SOP/Mic
 BF Biosciences Limited Doc. No. : Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
6 of 6

DESTRUCTION OF CULTURE MEDIA

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

NA

10. HISTORY
This is the First version.
- First Issue : JUN,2009
- Reason of composition / review new SOP.

Microbiology
BF/SOP/Mic