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M - 112
Beta Oxidation of Fatty Acid
7
Beta oxidation of fatty acids takes place in the
mitochondrial matrix for the most part. However,
fatty acids have to be activated for degradation by
coenzyme A by forming a fatty acyl-CoA thioester.
For short and medium length fatty acids, they undergo
this reaction in the mitochondria. The long chain fatty
acids can't go through the membrane though, so this
reaction occurs at the outer mitochondrial membrane
and the product has to be carried by carnitine across
the inner mitochondrial membrane. They are made
into acylcarnitine derivatives by carnitine transferase I
on the outer side of the inner membrane. These are
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acetyl-CoA for the even numbered fatty acids (without double bonds), and for those with an odd number of
carbons, it is 3-carbon propionyl-CoA instead.
For polyunsaturated fatty acids (with more than one cis-double bond) it goes through the same thing, but it
only goes through one more round of beta oxidation because then you get to a fatty acid with a trans and a
cis double bond. For this we use 2,4-dienoyl-CoA reductase to produce a trans-3-enoyl product which is
converted by an enoyl-CoA isomerase to a trans-2-enoyl-CoA which then goes normally through the
pathway. An example of this is on pg. 795 in the text book.
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FIGURE The Beta-oxidation pathway. (a) In each pass through this four-step sequence, one acetyl residue
(shaded in pink) is removed in the form of acetyl-CoA from the carboxyl end of the fatty acyl chain in this
example palmitate (C16), which enters as palmitoyl-CoA.
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