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cytoplasm
plasma
membrane
Fluid-Mosaic Model
Fluid Mosaic Model
Membrane is a mosaic of
Phospholipids
Glycolipids
Sterols
Proteins
Most phospholipids and some proteins can
drift through membrane
Fluid-Mosaic Model
Fluid the plasma membrane is the consistency of olive oil at body
temperature, due to unsaturated phospholipids. (cells differ in the
amount of unsaturated to saturated fatty acid tails)
Most of the lipids and some proteins drift laterally on either side.
Phospholipids do not switch from one layer to the next.
Cholesterol affects fluidity: at body temperature it lessens fluidity by
restraining the movement of phospholipids, at colder temperatures it
adds fluidity by not allowing phospholipids to pack close together.
Plasma
cytoskeletal proteins just Membrane
beneath the plasma
membrane
Phospholipid
Phospholipid Bilayer
Proteins Embedded in Lipid Bilayer
Nonpolar regions of the lipid bilayer
lock proteins into membranes
Fig. 4.7
Membrane Carbohydrates
Cell-cell recognition: Glycolipid: Covalently
Cells ability to distinguish bonded to lipids.
one cell from another. Glycoprotein: Most are
Is a crucial process for bonded to proteins.
cells.
Human blood type is
Recognition occurs when
binding b/w cells occurs to dependent on membrane
surface molecules (carbs.) carbohydrates (A, B, AB,
Usually short, branched O) on red blood cells.
chains of 15 or less
sugars.
Structure of the Plasma Membrane
Phospholipid bilayer
Phospholipid
Hydrophilic head
Hydrophobic tails
Cholesterol
Proteins
Transmembrane/
Intrinsic/Integral
Peripheral/Extrinsic
Cytoskeletal filaments
Carbohydrate chain
Glycoproteins
Glycolipids
Proteins
Membrane Proteins
Two major protein
transmembrane structures
2. -barrel
- outer membranes of Gram-
negative bacteria, chloroplasts, and
mitochondria
Proteins of the Plasma Membrane
Provide 6 Membrane Functions:
1) Transport Proteins
2) Receptor Proteins
3) Enzymatic Proteins
4) Cell Recognition Proteins
5) Attachment Proteins
6) Intercellular Junction
Proteins
Transmembrane Proteins
Top of protein is N-
terminus.
Bottom left is C-
terminus.
This is a ribbon model
of the protein
(secondary structure)
1) Transport Proteins
Channel Proteins
channel for lipid
insoluble molecules
and ions to pass freely
through
Carrier Proteins bind
to a substance and
carry it across
membrane, change
shape in process
2) Receptor Proteins
Bind to chemical
messengers (Ex.
hormones) which
sends a message into
the cell causing
cellular reaction
3) Enzymatic Proteins
Carry out enzymatic
reactions right at the
membrane when a
substrate binds to the
active site
4) Cell Recognition Proteins
Glycoproteins (and
glycolipids) on
extracellular surface
serve as ID tags
(which species, type of
cell, individual).
Carbohydrates are
short branched chains
of less than 15 sugars
5) Attachment Proteins
- Attach to cytoskeleton (to
maintain cell shape and stabilize
proteins) and/or the extracellular
matrix (integrins connect to
both).
- Extracellular Matrix protein
fibers and carbohydrates
secreted by cells and fills the
spaces between cells and
supports cells in a tissue.
- Extracellular matrix can
influence activity inside the cell
and coordinate the behavior of
all the cells in a tissue.
6) Intercellular Junction Proteins
Bind cells together
Tight junctions
Gap junctions
Types of Cell Junctions
Tight Junctions
Desmosomes
Gap Junctions
Tight Junctions
Transmembrane Proteins of opposite cells
attach in a tight zipper-like fashion
No leakage
Ex. Intestine, Kidneys, Epithelium of skin
Desmosomes
Cytoplasmic plaques of two cells bind with
the aid of intermediate filaments of keratin
Allows for stretching
Ex. Stomach, Bladder, Heart
Gap Junctions
Channel proteins of opposite cells join together
providing channels for ions, sugars, amino
acids, and other small molecules to pass.
Allows communication between cells.
Ex. Heart muscle, animal embryos
How do materials move into and
out of the cell?
Materials must move
in and out of the cell
through the plasma
membrane.
Some materials
move between the
phospholipids.
Some materials
move through the
proteins.
Plasma Membrane Transport
Molecules move across the plasma
membrane by:
What are three types of
passive transport?
1) Diffusion
2) Facilitated Diffusion
3) Osmosis
ATP energy is not
needed to move the
molecules through.
Passive Transport 1: Diffusion
Molecules can move directly
through the phospholipids of the
plasma membrane
This is called
What is Diffusion?
http://www.biologycorner.com/resources/diffusion-animated.gif
Transporters vary enormously in their architecture and size: from
small organic molecules and peptides to multi-subunit complexes (the
V-type and Fo-type ATPase may have more that 10 subunit in a
complex). The size of the individual molecules could be as large as
5000 amino-acid residues (Ryanodine receptor)
I. Simple
(Passive)Diffusion
no carriers is involved
Molecules that are transported through the
cell membrane via simple diffusion include
organic molecules, such as benzene and
small uncharged molecules, such as H2O,
O2, N2, urea, glycerol,and CO2
Protein-translocators - (Band
3, porins, erythrocyte glucose
transporter)
Channels - channels-forming
ionophores (gramicidin)
- voltage-gated channels (Na+-,
K+- and Ca2+ -channels)
- ligand-gated channels
- mechanosensitive channels
Active transport
- energy-dependent, against
concentration gradient
Primary Active Transport - utilizes energy of
ATP hydrolysis
Uniporters (mitochondrial
Ca2+ uniporter and NH+4-
transporter in plants require
H+ gradient)
Thermodynamics of
membrane transport
Simple (passive) diffusion
is a non-mediated and non-saturable
transport
Molecules that are transported through the cell membrane via simple diffusion
include small organic molecules, H2O, O2, N2, urea, glycerol,and CO2
Applications of simple diffusion: drugs delivery, analysis of membrane topology
using membrane-permeable and impermeable reagents, regulation of osmotic
pressure, etc.
Simple diffusion of molecules through
the membrane thermodynamically
resembles chemical equilibrium.
+
- chemical and electrical components
+ - +
chemical electrical
+ +
- - DG= RTln([C2]/[C1]) +
ZFDY
- -
+
- +
+ - -
DG - electrochemical potential
C2 and C1 concentrations of the
molecule
Y2-Y1=DY < 0
Z- ionic charge of the molecule
Z= + 1
T - absolute temperature
ZF DY < 0 R - gas constant
F - Faraday constant
DY - membrane potential
Facilitated diffusion - transport of molecules in an energy-
independent
fashion down the electrochemical gradient
Protein or carrier-mediated
A potassium ionophore
Gramicidin A:
a 15-mer polypeptide composed of alternating L and D amino-acids
forms a b-helix (6,7 amino acid residues per turn), which then dimerizes
head-to head by hydrogen bonding association between their N-formyl
ends to cross the membrane. The diameter of the pore is 4 A.
greatly increases permeability for monovalent cations, but not divalent
cations; i.e. it is less selective than valinomycin, but much more permeable
What molecules pass through the
plasma membrane by diffusion?
Gases (oxygen, carbon
dioxide)
Water molecules (rate
slow due to polarity)
Lipids (steroid
hormones)
Lipid soluble molecules
(hydrocarbons, alcohols,
some vitamins)
Small noncharged
molecules (NH3)
Why is diffusion important to cells
and humans?
Cell respiration
Alveoli of lungs
Capillaries
Red Blood Cells
Medications: time-
release capsules
Passive Transport 2: Facilitated
Diffusion
Molecules can move through the
plasma membrane with the aid of
transport proteins
This is called
What is Facilitated Diffusion?
Facilitated diffusion
is the net movement
of molecules from a
high concentration to
a low concentration
with the aid of
channel or carrier
proteins.
What molecules move through the plasma
membrane by facilitated diffusion?
Ions
(Na+, K+, Cl-)
Sugars
(Glucose)
Amino Acids
Small water
soluble molecules
This is called
What is Osmosis?
1) Active Transport
1) Primary
2) Secondary (no ATP)
2) Bulk Transport
1) Exocytosis
2) Endocytosis
1) Phagocytosis
2) Pinocytosis
3) Receptor-Mediated ATP energy is
endocytosis required to move the
molecules through.
Active Transport
Molecules move from areas of low
concentration to areas of high concentration
with the aid of ATP energy.
Requires protein carriers called Pumps.
The Importance of Active Transport
Bring in essential molecules: ions,
amino acids, glucose, nucleotides
Rid cell of unwanted molecules (Ex.
sodium from urine in kidneys)
Maintain internal conditions different
from the environment
Regulate the volume of cells by
controlling osmotic potential
Control cellular pH
Re-establish concentration
gradients to run facilitated diffusion.
(Ex. Sodium-Potassium pump and
Proton pumps)
The Sodium-Potassium Pump
3 Sodium ions move out of
the cell and then 2
Potassium ions move into
the cell.
Driven by the splitting of
ATP to provide energy and
conformational change to
proteins by adding and then
taking away a phosphate
group.
Used to establish an
electrochemical gradient
across neuron cell
membranes. http://www.biologie.uni-hamburg.de/b-online/library/biology107/bi107vc/fa99/terry/images/ATPpumA.gif
Secondary Active Transport
Via Facilitated Diffusion of Na
Counter Transport the transport
of two substances at the same time
in opposite directions, without ATP.
Protein carriers are called
Antiports.
Co-transport the transport of
two substances at the same time in
the same direction, without ATP.
Protein carriers are called
Symports.
Gated Channels receptors
combined with channel proteins.
When a chemical messenger binds
to a receptor, a gate opens to allow
ions to flow through the channel.
Bulk Transport: Exocytosis
Movement of large
molecules bound in
vesicles out of the cell
with the aid of ATP
energy. Vesicle fuses
with the plasma
membrane to eject
macromolecules.
Ex. Proteins,
polysaccharides,
polynucleotides, whole
cells, hormones, mucus,
neurotransmitters, waste
Bulk Transport: Endocytosis
Movement of large molecules into the cell
by engulfing them in vesicles, using ATP
energy.
Three types of Endocytosis:
Phagocytosis
Pinocytosis
Receptor-mediated endocytosis
Phagocytosis
Cellular Eating engulfing large
molecules, whole cells, bacteria
Ex. Macrophages ingesting bacteria or worn
out red blood cells.
Ex. Unicellular organisms engulfing food
particles.
Pinocytosis
CellularDrinking engulfing liquids and
small molecules dissolved in liquids;
unspecific what enters.
Ex. Intestinal cells, Kidney cells, Plant root
cells
Receptor-Mediated Endocytosis
Movement of very specific
molecules into the cell with the
use of vesicles coated with the
protein clathrin.
Coated pits are specific
locations coated with clathrin
and receptors. When specific
molecules (ligands) bind to the
receptors, then this stimulates
the molecules to be engulfed
into a coated vesicle.
Ex. Uptake of cholesterol (LDL)
by animal cells
Review Types of Endocytosis
What is phagocytosis?
What is pinocytosis?
Whatis receptor-
mediated endocytosis?
Overview of
Membrane Proteins
Adhesion Communication
Proteins Proteins
Fig. 5.6, p.78
Overview of
Membrane Proteins
Stepped Art
Fig. 5.7a, p.80
Diffusion
Stepped Art
Fig. 5.7b, p.80
Factors Affecting
Diffusion Rate
Steepness of concentration gradient
Steeper gradient, faster diffusion
Molecular size
Smaller molecules, faster diffusion
Temperature
Higher temperature, faster diffusion
Electrical or pressure gradients
Membrane Crossing
Mechanisms
Diffusion across lipid bilayer
Passive transport
Active transport
Endocytosis
Exocytosis
Cell Membranes Show
Selective Permeability
oxygen, carbon glucose and other large,
dioxide, and other polar, water-soluble
small, nonpolar molecules; ions (e.g.,
molecules; some H+, Na+, K+, Ca++,
water molecules Cl); water molecules
Concentration
gradient across
cell membrane
ATP
Low
Passive
transport proteins allow solutes to
move both ways
low
Stepped Art
ATP
Pi
Stepped Art
ADP
Fig. 5-11, p.83
Osmosis
Diffusion of water molecules across a
selectively permeable membrane
water molecules protein molecules
Direction of net flow is
determined by water
concentration gradient
p.84
Tonicity
Refers to relative solute concentration of
two fluids
Hypotonic - having fewer solutes
2% sucrose
solution
1 liter of 1 liter of
1 liter of 10% sucrose 2% sucrose
distilled water solution solution
first second
compartment compartment
hypotonic hypertonic
solution solution
membrane fluid volume
permeable to rises in
water but not to second
solutes compartment
d c
f
e
parasite macrophage
Golgi body
endoplasmic reticulum
lipid
bilayer
cytoskeletal
proteins cytoplasm
Cholesterol
(steroid), wedged b/w
phospholipids has different effects on
membrane fluidity.
Warm temps. cause it to be less fluid by
restraining phospholipid movement.
Lowers the temp. for membranes to solidify.
Membranes must be fluid to work properly.
Solidified membranes change permeability,
causing enzymatic proteins to function
improperly.
Membrane Fluidity
Membrane Fluidity (cont.)
human mouse
cell cell
fusion into
hybrid cell
proteins from
both
in fused Stepped Art
membrane Fig. 5.5b, pg. 77
Studying Membranes
Stepped Art
Fig. 5.5a, pg. 77
Freeze-Fracture
Method of preparing cells for
microscopy.
The frozen cell is fractured down
the middle of the membrane
bilayer
The proteins do not split, but
follow either of the membrane
layers
A mist of platinum is sprayed onto
the surface at an angle creating
shadows indicating depth.
Carbon is added to strength and
the specimen is digested, leaving
the platinum-carbon film which is
then examined by electron
microscopy.
When viewed, interior layer looks
cobblestoned, w/ proteins
dispersed in matrix.