REPORT OF

Determination of Vitamin Level in IPI Vitamin C

Compiled to fulfill one of the tasks in Thirteen Vocational High School Bandung majoring
in Chemical Analysis
English Lesson
Teacher: Indira Sari Paputungan, M. Ed

DISUSUN OLEH:
DWI GUMILANG
NIS: 101415212
FITHRI LISA ARIYANTI
NIS: 101415230
HILMAN PRAYOGA
NIS: 101415242
TRI PUJI LESTARI
NIS: 101415344

CLASS XIII AK2

GOVERNMENT OF BANDUNG CITY

EDUCATION AUTHORITIES
THIRTEEN VOCATIONAL HIGH SCHOOL
MAJORING : 1. CHEMICAL ANALYSIS; 2. COMPUTER TECHNIQUES
NETWORKS; 3. SOFTWARE ENGINEERING

Soekarno-Hatta Street KM. 10 Bandung 40286: Telp/Fax (022)7318960

Home page: www.smkn-13bdg.sch.id

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 ACKNOWLEDGEMENTS

All praise and gratitude delivered to Allah SWT who has given blessing, grace and gifts so that
the writing of the report entitled "Determination of Vitamin C in IPI Vitamin C with Iodimetric
Titration" can work well. On this occasion we would like to extend our gratitude to:
Allah SWT who has done everything.
Mrs. Fatni Rifqiyati and Miss. Syafitri K. Arief as analytical chemistry analysts who
have provided much guidance and direction during the research of this report.
Mrs. Indira Sari Paputungan as the English language teacher who has provided much
guidance and direction during the writing of this report.
Through this experiment we hope to give information to the public about the content of IPI
Vitamin C.

Bandung, 4 Oktober, 2017

Writer

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 CONTENTS

ACKNOWLEDGEMENTS .......................... Error! Bookmark not defined.

CONTENTS ................................................................................................ iii
ABSTRACT ................................................................................................. 1
CHAPTER 1 ................................................................................................ 2
A. INTRODUCTION ........................................................................................ 2
B. METHODOLOGY ..................................... Error! Bookmark not defined.
CHAPTER 2 .................................................. Error! Bookmark not defined.
A. RESULTS AND FINDINGS ....................................................................... 4
B. DISCUSSION .............................................................................................. 6
CHAPTER 3 ................................................................................................ 7
A. CONCLUSIONS .......................................................................................... 7
B. RECOMMENDATIONS ............................................................................. 7
REFERENCES ............................................................................................ 8
GLOSSARY OF TECHNICAL TERMS .................................................. 9

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 ABSTRACT

This experiment aims to determine the levels of vitamin C in the IPI Vitamin C. The
method used is Iodimetric titration with the principle of redox titration. Starch was added
to the samples that act as an indicator. The sample is titrated with I2(Iodine), which ended
with the onset of the color blue in the sample. From the experimental results and the
discussion that has been done, it can be concluded that (1) the mass of vitamin C in one
tablet is 47.9177 mg Vitamin C IPI grading 94.42% per caplet.

Key words: Iodimetric, iodine, redox titration, starch, vitamin C.

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 CHAPTER 1

INTRODUCTION
In everyday life people need carbohydrates, proteins, fiber, fats, vitamins and minerals.
One that plays an important role for the body is vitamins which include vitamins A, B, C,
D, E, and K.

This time we will discuss about Vitamin C. vitamin is not just energy, but vitamin do
regulator function (regulator) which cooperate with enzyme in reaction of biochemistry.
Vitamin C or ascorbic acid is a valuable component in food because it is useful as an
antioxidant and contains medicinal properties.

Vitamin C is generally found only in vegetable and fruits such as oranges, pineapples,
papaya, tomatoes and garlic.

METHODOLOGY
The method used is to conduct quantitative experiments in the laboratory. The
experiments were conducted in Analytical Chemistry Laboratory, Chemistry Analysis
Department, Thirteen Vocational High School Bandung, on Tuesday, November 17,
2016. The tools used in the experiment were Erlenmeyer flask 250 mL; beaker glass 100
mL; dropper pipette; stative and clamps; burette; funnel; measuring cup; mortar and
pestle; and watch glass.

The material used is vitacimin;

1. IPI VitaminC; iodine solution 0.1 N.
2. 1%; indicator of starch; and aquadest.

The working procedure of this experiment is as follows.

1. Weigh 20 vitamin C tablets equivalent to 250-300 mg of vitamin C.
2. Insert into a sharpened Erlenmeyer flask.
3. Add 25 mL of aquadest and 2 mL of 1% starch.
4. Titrate with standard I2 solution to the end point of clear blue color.

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5. Read volume usage.
6. Duplicate
7. Calculate levels of vitamin C.

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 CHAPTER 2

RESULTS AND FINDINGS

This practice aims to determine the levels of vitamin C in IPI Vitamin C. The method
used is titration of Iodimetric with redox titration principle. Step the first thing to do is
sample preparation. Solid IPI Vitamin C samples were mashed using mortar and pestle.
One tablet of Vitamin C IPI has a weight of 0.2514 grams. After smoothing, 1.2570
grams of powdered sample is dissolved with 25 mL of aquadest. The sample after
dissolving is yellow (Picture 1).

Picture 1. The sample before being titrated is yellow.

After the sample preparation is completed, Iodimetric titration of the sample is then done.
A total of 1.2570 grams of sample is fed into Erlenmeyer flask, then add 25 mL of
aquadest. Then added 3 drops of starch. Each sample was titrated using solution I2. The
titration process is terminated after the solution changes color to blue (Picture 2).

Picture 2. The solution is blue at the end point of the titration.

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 Table 1. Volume I2 used in Vitacimin titration.
No Sample volume Titrant volume
(mL) (mL)
1 25 mL 32.80 mL
2 25 mL 32.78 mL

Calculation 1. Vitamin C levels for titrant volume 32.80 mL.

20 5028.7
= = = 251.435
20 20
250
=
50

250
= 251.435 = 1257.175 = 1.2572
50

1295
= = = 5.1504
251.435

= ( )2 6 8 6

= (32.80 0.0830 ) 88.06 = 239.7345

239.7345
= = = 46.5468
5.1504

46.5468
= 100% = = . %
50

Calculation 2. Vitamin C levels for titrant volume 32.78 mL.

20 5028.7
= = = 251.435
20 20
250
=
50

250
= 251.435 = 1257.175 = 1.2572
50

1258.3
= = = 5.0045
251.435

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 = ( )2 6 8 6

= (32.78 0.0830 ) 88.06 = 239.5884

239.5884
= = = 47.8746
5.0045

47.8746
= 100% = = . %
50

DISCUSSION
In this experiment, determination of the level of vitamin C was done by Iodimetric
method. Iodimetric is a direct titration and is a quantitative determination or
determination method where the basis of the determination is the amount of I2 that reacts
with the sample or is formed from the reaction between the sample and the iodide ion. In
redox reactions, there must always be oxidizing and reducing agents, because when an
element increases its oxidation number (releasing electrons), there must be an element
which oxidation number is reduced or decreased (electron capture).

The sample used in this experiment was vitamin C under the IPI brand. The indicator used
is the starch indicator. Starch is used because it will form a dark blue amorphous iod
complex even though the concentration of I2 is very small.

At the time of titration, I2 will be reduced to I- because it reacts with vitamin C which
causes the brown-colored color to become clear. Then after all vitamin C reacts with I2,
the excess I2 will react with the starch to form a blue complex that signifies the endpoint
of the titration.

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 CHAPTER 3

CONCLUSIONS
From the experimental results and the discussion that has been done it can be concluded
(1) the average weight of vitamin C in a vitamin C IPI is 47.2107 mg.

RECOMMENDATIONS
Experiment should be done thoroughly and carefully especially in terms of keeping the
bottle of vitamin C that will be used in the experiment so that it will not be oxidized.

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 REFERENCES

http://www.academia.edu/7445092/Penentuan_Kadar_Vitamin_C_pada_Vitacimin_dan
_UC-1000_dengan_Titrasi_Iodimetri

Andarwulan, Nuri, Sutrisno Koswara. 1989. Kimia Vitamin. Jakarta: Rajawali

Pers. Cooper, Kenneth H. 2001. Sehat Tanpa Obat: Empat Langkah Revolusi Antioksidan
yang Mengubah Hidup Anda. Bandung: Kaifa

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 GLOSSARY OF TECHNICAL TERMS

IODYMETRY - a method of quantitative analysis of volumetric based on

redox in which the compound and reactor react directly or
often called Direct Titration.

IODINE - a chemical element with symbol I and atomic number 53.

REDOX TITRATION - a type of titration based on a redox reaction between

the analyte and titrant.

STARCH - a polymeric carbohydrate consisting of a large number

of glucose units joined by glycoside bonds.

VITAMIN C - a vitamin found in food and used as a dietary supplement.

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