VIII.

Data Hasil Pengamatan

No. Konsentrasi Arsorbansi
1. Blanko 0,00
2. 1 ppm 0,19
3. 2 ppm 0,20
4. 3 ppm 0,22
5. 4 ppm 0,26
6. 5 ppm 0,35
7. Sampel 0,34
IX. Perhitungan
Asam Askorbat 20 ppm 5 mL
1. Asam askorbat 1 ppm
N1 . V1 = N2 . V2
20 ppm . V1 = 1 ppm . 25 mL
1 ppm . 25 mL
V1 =
20 ppm
V1 = 1,25 mL

2. Asam askorbat 2 ppm

N1 . V1 = N2 . V2
20 ppm . V1 = 2 ppm . 25 mL
2 ppm . 25 mL
V1 =
20 ppm
V1 = 2,5 mL

3. Asam askorbat 3 ppm

N1 . V1 = N2 . V2
20 ppm . V1 = 3 ppm . 25 mL
3 ppm . 25 mL
V1 =
20 ppm
V1 = 3,75 mL

4. Asam askorbat 4 ppm

N1 . V1 = N2 . V2
20 ppm . V1 = 4 ppm . 25 mL
4 ppm . 25 mL
V1 =
20 ppm
V1 = 5 mL
 5. Asam askorbat 5 ppm
N1 . V1 = N2 . V2
20 ppm . V1 = 5 ppm . 25 mL
5 ppm . 25 mL
V1 =
20 ppm
V1 = 6,25 mL

NO X(ppm) Y(A) X.Y X2 Y2

1 1 0,19 0,19 1 0,0361
2 2 0,20 0,4 4 0,04
3 3 0,22 0,66 9 0,0484
4 4 0,26 1,04 16 0,0676
5 5 0,35 1,75 25 0,1225
15 1,22 4,04 55 0,3146

nxy - x.y
Slope(A) =
nx2 - (x)2
5(4,04)-(15)(1,22)
=
5(55) - (15)2
20,2 - 18,3
=
275 - 225
1,9
= = 0,038
50

x2 .y - xy.x
Intersept(B) =
nx2 - (x)2
55(1,22)-(4,04)(15)
=
5(55) - (15)2
67,1 - 60,6
=
275 - 225

6,5
= = 0,13
50
 xy - x.y
n
r = 2
(x) y
(x2 - ) . (y - )
n n
(4,04) - (15) (1,22)
5
=
(15)2 1,22
(55 - ) . (1,22 )
5 5

-2,852
=
2,44
r = - 1,168

y = Ax+B
Dimana y = absorbansi B = intersept
A = slope X = konsentrasi asam askorbat
Maka dari persamaan y = Ax+B
Y= Ax+B
Y-B 1,22 - 0,13 1,09
X= = = = 28,68
A 0,038 0,038
ppm = mg/l
mg = ppm.L
mg = 28,68 mg/L.0,001 L
= 0,028 mg
= 2,8 x 10-4 g

% Vitamin C = g/L x 100%

2,8 x 10-4 g
= x 100%
0,001 L
= 0,28 x 100%
= 28%