XII.

Attachment

Picture Note
Tools that need in this experiment are :
12 pieces of Test tubes
2 pieces of Beaker glass
1 piece of Graduated cylinder 10 mL
1 piece of Volumetric flask 10 mL
1 piece of Funnel
1 piece of Stirring rod
1 piece of Rack test tube

Materials that need in the experiment are :

1-5 mL of Saliva
Aquadest
solution of I2
Starch solution 0,4 mg/mL
Starch solution in pH 1; 3; 5; 7; 9

One of our team try to take out her saliva

 Dissolved 1 mL saliva in 10x; 20x;
30x; 40x; 50x using aquadest

The Effect of pH in Enzyme Activity

Put 1 mL amylum solution in pH 1;

3; 5; 7; 9 in each test tube and 1 mL
amylum solution in blanco test tube

After 2 minutes, added 10 drops of

aquadest in blanco tubes and 10
drops of enzyme solution in 5 test
tubes
 Heated in waterbath 35C-40C for
5 minutes

Added 2 10 drops of I2, the colour

changes into blue
Blanco = blackish blue (+++)
pH 1 = blackish blue (++)
pH 3 = blackish blue (+)
pH 5 = blackish blue (-)
pH 7 = blackish blue (--)
pH 9 = blackish blue (---)
Added 6 mL of aquadest, the
colour change
Blanco = light blue (-)
pH 1 = light blue
pH 3 = light blue (+)
pH 5 = light blue (++)
pH 7 = light blue (+++)
pH 9 = light blue (++++)
 Read the absorbance in 680 nm
Blanco = 0,219
pH 1 = 0,256
pH 3 = 0,168
pH 5 = 0,148
pH 7 = 0,162
pH 9 = 0,131

The Effect of Concentration in Enzyme Activity

Put 1 mL amylum solution in 6 test

tubes and let for 2 minutes

After 2 minutes, added 10 drops of

aquadest in blanco tubes and 10
drops of enzyme solution 10x, 20x,
30x, 40, 50x in 5 test tubes

After 2 minutes, heated in

waterbath 70C for 5 minutes
 Added 2 10 drops of I2, the colour
changes into blue

Added 6 mL of aquadest, the

colour change
Blanco = light blue (++)
10 x = light yellow
20 x = yellow
30 x = orange
40 x = light blue
50 x = light blue (+)
Read the absorbance in 680 nm
Blanco = 0,378
10 x = 0,094
20 x = 0,106
30 x = 0,131
40 x = 0,323
50 x = 0,389