72 Registration No. QUESTION PAPER SERIES CODE Centre of Exam. A same of Candas: Signature of Invigilator ENTRANCE EXAMINATION, 2015 Pre-Ph.D./Ph.D. in MOLECULAR MEDICINE, [ Field of Study Code : CMMP (169) | Time Allowed : 3 hours Maximum Marks : 70 INSTRUCTIONS FOR CANDIDATES Candidates must read carefully the following instructions before attempting the Question Paper : (i) Write your Name and Registration Number in the space provided for the purpose on the top of this Question Paper and in the Answer Sheet. (i) Please darken the appropriate Circle of Question Paper Series Code on the Answer Sheet. {ii The Question Paper is divided into two Parts : Part—A and Part—B, Both Parts have multiple-choice ‘questions. All answers are to be entered in the Answer Sheet provided with the Question Paper for the purpose by darkening the correct choice, i., (a) or (b) orc) or (d) with BALLPOINT PEN only against each question in the corresponding Circle (iv) Part—A consists of 30 questions and all are compulsory. (v) Part—B contains 60 questions. Answer any 40 questions, In case any candidate answers more than the required 40 questions, the first 40 questions attempted will be evaluated, (vi) Each correct answer carries 1 mark. There will be negative marking and % mark will be deducted for each wrong answer. (vi) Answer written by the candidates inside the Question Paper will not be evaluated. (vii Calculators and Log Tables may be used {ix) Pages at the end have been provided for Rough Work. {a} Return the Question Paper and Answer Sheet to the Invigilator at the end of the Entrance Examination. DO NOT FOLD THE ANSWER SHEET. INSTRUCTIONS FOR MARKING ANSWERS 1, Use only Biue/Black Ballpoint Pen (do not use pencil) to darken the appropriate Circe. 2. Please darken the whole Circle. 3. Darken ONLY ONE CIRCLE for each question as shown in example below Wrong Wrong Wrong Wrong Correct ©000|GHOO|GBHOG|OHOO|OHOO Once marked, no change in the answer is allowed. Please do not make any stray marks on the Answer Sheet. Please do not do any rough work on the Answer Shect Mark your anewer only in the appropriate space against the number corresponding to the question. Ensure that you have darkened the appropriate Circle of Question Paper Series Code on the ‘Answer Sheet. 172-A(72-41 (72-8 PART—A Answer all questions ‘The pH of 0-1 Maqueous solution of an acid is 3. What is its degree of dissociation? (a) 1% (b) 10% () 50% (a) 25% Calculate the molality of a solution that has 1-5 moles added to 675 mL of solvent @) 10M () 11M () 20M (@) 22M ‘Thermal stability of alkaline earth metal carbonates decreases in order (a) BaCO, > SrCO, > CaCO, > MgO, (b) BaCO, > SrCO, >MgCO, > CaCO, (c) MgCO, > CaCO, > SrCO, > BaCO, (a) CaCO, > MgCO, > SrCO, > BaCO, Which of the following metalloproteins is a redox protein? (a) Carbonic anhydrase (>) Ferritin ()_ Cytochrome ¢ (@) Transferrin 3 [P.T.0.5. Match the following and indicate the set showing the correct matching : P. Ferritin 1. Electron transport Q. Vitamin B,» IL. Tonophore R. Cytochromes _ll._ Organometallic compound S. Valinomycin IV, Iron storage Codes (Pe tO aR von om ) P Q Rk Ss lomo woo @ P Q Ros m ow @ P QR s vot 6. For Clustal W2, a multiy be ple sequence alignment programme, the input sequence could (@) only DNA sequence (b) only amino acid sequence (¢) either DNA or amino acid sequence (a) None of the above hormone is (@) oxytocin (>) insulin (¢) gibberellin (@) estrogen (72-88. _B-ZIP type of transcription factors are found in all organisms. While the basic amino acid residues (B) are required for DNA binding, the leucine zipper (ZIP) is essential for dimerization of the protein. The order in which they appear in the protein is followed as (@) BZIP (b) B-ZP-B () B-ZIP-ZIP-B (4) ZIP-B 9. The percentage errors in the measurement of mass and speed are 2% and 3% respectively. The error in the estimation of kinetic energy obtained by measuring mass and speed will be fa) 12% (b) 10% () 2% (a). 8% 10. Which of the following molecules can act as an oxidizing as well as reducing agent? (@) HS () SO, (6) HO. (@ Fy 11. The half-life of the reaction N20; —»2NO, + 40z is 24 h at STP. Starting with 10-8 g of N05, how much oxygen will be obtained after a period of 96 h? @ 15L (b) 336L (©) 105 (a) 0074 1A 5 [P.7.0.12. The solubility product of As,03 is 10-8x10°. It is 50% dissociated in saturated solution. The solubility of salt is fa) 1072 (o) 2x10? ( sx108 (a) 5-4x10°° 18. The escape velocity from the earth is 11:2 km/s. The escape velocity from a planet having twice the radius and same mean density as the earth is (@) 56 km/s (b) 112 km/s () 22-4 km/s (@) 448 km/s 14. Ram walks at 10 km/h. If he would have walked at 14 km/h, he would have walked 20 km more. The actual distance travelled by him is (@) 50 km (0) 56 km () 70 km (@) 80 km wr Geitand he take aa 2m i fea ke, Amano (@) 12 kg (b) 60 kg () 72 kg (4) 96 kg /72-A16. 17. 18. 19. /72-A Which one of the following is the odd number in the series 396, 462, 572, 427, 671, 2647 fa) 396 (b) 427 (671 (a) 264 Manjul can donate blood to Kamran and Rajesh. But Kamran can donate blood to Rajesh and not to Manjul. Rajesh can receive blood from both Manjul and Kamran Which of the following statements about the blood groups of the individuals is true? (a) | Manjul has blood group O, Rajesh has blood group AB and Kamran has either blood group A or B. () Manjul has blood group AB, Rajesh has group O and Kamran has group A (c) Manjul has blood group O, Rajesh has group B and Kamran has group A (@) Both (b) and (c) How many triangles in total can you find in the following figure? (9 () 10 @ 12 (@) 13 Ampicillin is one of the most commonly used antibiotic. It acts as bacteriocidal (@) interfering with 70S ribosomes resulting in incorrect translation of mRNA (b) interfering with translation through binding to 30S ribosomal subunits (c) interfering with cell wall biosynthesis in growing bacteria (a) All of the above 7 [P.T.O.20. 22, 172A Four balls of different colours are kept in a row. The red ball is kept next to the green ball but not next to the blue ball. Which ball is next to the yellow ball? (a) Red ball (b). Green ball (©) Blue ball (4) Red and green balls Match the following marker enzymes in the correct order with their subcellular fraction/compartment : (i) acid phosphatase, (i) catalase, (ii) glucose-6-phosphatase and (jv) lactate dehydrogenase. e (a) Microsomes, peroxisomes, lysosomes, cytosol respectively (b) | Cytosol, microsomes, peroxisomes, lysosomes respectively (©) Peroxisomes, lysosomes, microsomes, cytosol respectively (@) Tysosomes, peroxisomes, microsomes, cytosol respectively Which of the following sets of organelles is normally self-replicating? (a) Ribosomes and vacuole () Nucleus and ribosomes () Mitochondria and chloroplast (@) All of the above23, 24, 25. 26. 172-8 ‘The period of revolution of an earth's satellite close to the surface of the earth is 90 min. The time period of another satellite in an orbit at a distance of four times the radius of the earth from its surface will be fa) (b) ©) (a) 225 min 360 min 90 min 720 min Which of the following is true about viruses? fa) (b) (o) (@) ‘They are not composed of cells As virions they cannot metabolize nutrients ‘They contain DNA or RNA All of the above Which of the following statements is true about the Indian National Anthem ‘Jana-Gana-Mana’? (@) (b) () (a) ‘The original ‘Jana-Gana-Mana’ is a five-stanza hymn from which the first stanza is adopted as the National Anthem of India, December 27, 2011 marked the completion of 100 years of Jana-Gana-Mana’ since it was sung for the first time. A formal rendition of Jana-Gana-Mana’ takes fifty-two seconds. All of the above ‘The hormone that does not involve a second messenger for initiating its signalling cascade is (a) glucagon (b) follicle stimulating hormone (FSH) (c) (a) luteinizing hormone (LH) estrogen 9 [P.T.0.a7. 28, 29, 30. /72-4 Multidrug resistance protein 1 (MDR1), also known as p-glycoprotein (permeability Slycoprotein], is an important protein of the cell membrane that pumps many foreign substances out of the cells. It plays a defensive role against diverse harmful substances. Which of the following statements is true about MDR1? (@) It forms an ATP-dependent efflux pump with broad substrate specificity. (b) It is present in bacteria, fungi and animals. (<) lis higher expression may be responsible for drug resistance and failure of drug therapy. (@)_ All of the above Which of the following is the most commonly mutated oncogene in cancer? (a) myc (>) ras (c) BRCA (d) p53 In a clinical blood test, the el levels diseased state of” n° “l*vated levels of serum alkaline phosphatase indicate the (a) liver (b) salivary glands () spleen (@) lung Which one of the following is not an RNA Polymerase Il] subunit? fa (b) ot 6 @ o 1031. 32. 33. 34, (72-4 PART—B Answer any forty questions A bar of magnetic substance is placed in a magnetic field with its length making an angle of 30° with the direction of the magnetic field. How will the bar behave? (a) It will align itself perpendicular to the magnetic field (b) It will remain as before (c) It will align itself parallel to the magnetic field (a) Its behaviour cannot be predicted ‘A student grows an adherent mammalian cell line in a tissue culture plate. After 24 hours of culture, he/she adds 2 mM EGTA. What will be the consequence? (a) Cells will continue to grow normally (b) Cells will lose contact with the plate (c) Cells will lose contact with cach other (a) Cells will be arrested in G1 phase In which height above the surface of the earth the value of acceleration due to gravity would be half of ts value on the surface of the earth? (Radius of the earth is 6400 km) (a) 2561 km (&) 3200 km () 2650 km (a) 9800 km Which one of the following is not a plasmid? (a) RK2 (b) RPA () pBR322 (@ Mis ul [P.T.0.35. 36, 37. 38, (72-8 ‘A patient with rheumatic fever develops a sore throat from which B-hemolyi streptococci are cultured. The patient is started on treatment with penicillin, and the sore throat resolves within several days. However, 7 days after initiation of penicillin therapy the patient develops a fever of 103 °F, a generalized rash and proteinuria. This most probably resulted from (a) recurrence of the rheumatic fever (b) a different infectious disease (c)_ an IgG-lgM response to penicillin (a) a delayed hypersensitivity reaction to penicillin Each of the following statements concerning haptens is correct, except (a) @ hapten can bind to an antibody (0) a hapten cannot induce an antibody by itself; rather, it must be bound to a carrier protein to be able to induce antibody (¢) in both penicillin-induced anaphylaxis and poison ivy, the allergens are haptens (4) _haptens must be processed by CD8+ cells to become immunogenic Each of the following statements concerning immunologic tolerance is correct, except (a) tolerance is not antigen-specific, i.e., partial paralysis of the immune cells results in a failure to produce a response against many antigens (b) tolerance is more easily induced in T cells than in B cells (c) tolerance is more easily induced in neonates than in adults (@) tolerance is more easily induced by simple molecules than by complex ones On January 15, a patient developed an illness suggestive of influenza, which lasted 1 week. On February 20, she had a similar illness. She had no influenza immunization during this period. Her hemagglutination inhibition titer to influenza A virus was 10 on January 18, 40 on January 30, and 320 on February 20. Which one of the following is the most appropriate interpretation? (9) The patient was ill with influenza A on January 15 (b) The patient was ill with influenza A on February 20 (¢) The patient was not infected with influenza virus (4) The patient has an autoimmune discase 1239, 40. 41. 42, 172-A Proteins encoded in the nucleus and transported to the organelle like mitochondria should contain (@) signal peptide for targeting (b) transit peptide for crossing the membrane (c)__ protease site to get rid of the signal peptide (4) All of the above Patients with severely reduced C3 levels tend to have (2) increased number of severe viral infections (b) increased number of severe bacterial infections (c} low gamma globulin levels {d) frequent episodes of hemolytic anemia Which of the following Ebola virus species is not fatal among humans? (a) Zaire (0) Cote d'ivoire (© C. Bundibugyo (a) Reston ‘The C, plants are photosynthetically more efficient than C, plants, because (a) they have more chloroplasts (b) the CO, compensation point is more (c) COy generated during photorespiration is trapped and recycled through PEP carboxylase (a) the CO, efflux is not prevented 13 [P.rO,43, 45, (72-4 Where does bulk fixation of carbon through photosynthesis take place? (@) Crop plant (&) Ocean (©) Tropical rain forest (a) Both (a) and (¢) Seed dormancy is due to (@) ethylene (>) abscissic acid (IAA (@) starch Which of the following statements is incorrect regarding light reaction? (@) Light reaction is called as Hill reaction () co, (@) ATP and NADPH are produced in light reaction The enzyme for converting DNA with Send overhang into a blunt end is (2) Klenow fragment of DNA Polymerase () DNA polymerase 1 holoenzyme ()_ 74 DNA polymerase (@) S1 nuclease 1447. 49. 50. 172-8 Mifepristone, a well-established antiprogestin, has primary clinical application in (a) breast cancer treatment (0) cervical cancer (c) pregnancy termination (d) Both (a) and (b) Muscarinic receptor is a (a) G protein-coupled receptor (b) voltage-gated ion channel receptor (ligand-gated cation receptor (a) member of the nuclear receptor superfamily When a ligand activates a receptor to produce an action opposite to the established agonist action, the ligand is referred to as (a) antagonist (b) mixed agonist-antagonist (c) protagonist (@) inverse agonist PI of an amino acid is defined as the pH, where (a) the molecule carries no electric charge (b) the carbonyl group is uncharged (6) the molecule is having equal number of positive and negative charges (a) the molecule is having maximum electrolytic mobility 15 [PTO51. 52. 53, 54, (72-4 If egg white protein, ovalbumin, is denatured in a hard-boiled egg, which of the following is least affected? (a) Primary structure of ovalbumin (b) Secondary structure of ovalbumin (¢) Tertiary structure of ovalbumin (4) Quaternary structure of ovalbumin Insulin is a peptide hormone produced by ® cells that {@) stimulates gluconeogenesis and glycolysis (b) stimulates gluconeogenesis and inhibits alycolysis (©) inhibits gluconeogenesis and glycolysis (@) inhibits gluconeogenesis and stimulates elycolysis (@) Alanine (0) Lysine (6) Histidine (4) Glutamate In different types of tiss ue transplantation, is the rate of graft rejection in decreasing order (2) isograft > xenograft > allograft (©) allograft > isograft > xenograft (©) xenograft > autograft > allograft (9) xenograft > allograft > isogratt 1655. Formation of nonfunctional methemoglobin causes blue baby syndrome due to (@) excess of nitrates in drinking water (b) excess of arsenic concentration in drinking water (c) nutritional iron deficiency (@) hypoxic exposure during birth 56. An artificial pacemaker is implanted subcutaneously and connected to the heart in patients (a) having more than 90% blockage of the three main coronary arteries () with irregularity in the heart rhythm (c) suffering from arteriosclerosis (@) having a very high blood pressure 57. Continued consumption of a diet rich in red meat, eggs and butter for a long period may lead to (a) kidney stones (b) hypercholesterolemia (c) hepatic fibrosis (a) intestinal bowel disease 58. A baby has been bor with a.small tail, which is a case exhibiting (a) atavism (b) mutation (c)__ metamorphosis (@)__retrogressive evolution 172A 17 [P.T.0.59. The table below shows the comparison of the effects of sympathetic and "parasympathetic nervous system for four physiological systems. ‘The correct combination among the following is Physiological System | Sympathetic Nervous | Parasympathetic Nervous System System | lial Salivary gland Stimulates secretion —_| Inhibits secretion | (b) | Pupil of the eye Dilates Constricts (©) | Heart rate Decreases Increases (4) | Intestinal peristalsis | Stimulates Inhibits 60. Which one of the following pairs is correctly matched? (a) Oxyntic cells—Secrete fluid with pH between 2.0 and 3:0 (0) Alpha cells of islets of Langethans—Secrete fluid that decreases blood sugar level (©) Kupffer’s cells—Secrete enzymes that hydrolyze nucleic acids (d) Sebaceous glands—Secrete fluids that evaporate for cooling 61. Under which condition semiconservative DNA replication is not possible in E.coli? {@) When bacteria is treated with 2mM EDTA solution (©) When bacteria is treated with 0:1% Triton X-100 detergent (©) When 10 mM MgSO, solution is added to the growth medium (2) When proteinase K is added to the growth medium 62, Starting from left to right direction, the conse: (6) Transactivation domain, DNA-binding domain, Ligand-binding domain (@) Transactivation domain, Ligand-binding domain, DNA-binding domain IT2-A 1863. Tamoxifen is a classical example of selective estrogen receptor modulator (SERM) used in the treatment of breast cancer. Its nature as SERM implies that tamoxifen can bind estrogen receptor and (@) acts as a pure antagonist only (b) acts as a pure agonist only (¢) acts as an antagonist in one tissue and agonist in another (a) acts as an agonist and antagonist in the same cell or tissue 64. Which one of the following hormones is secreted by implanted blastocyst that acts on the corpus luteum in the ovary to stimulate the production of estrogen and Progesterone for the maintenance of uterine lining? (@) HCG (0) Oxytocin () Estrogen (a) Progesterone 65. Which of the following matches correctly? (a) Factor Il: Thromboplastin (0) Factor Ill : Prothrombin (c) Factor VIII : Antihemophilic globulin (@) Factor XII : Hemophilic 66. Which of the following is a correct statement? (a) Inferior vena cava receives deoxygenated blood from head and body. (b) Superior vena cava receives deoxygenated blood from the lower body and organs. () Pulmonary artery carries deoxygenated blood to the lungs. (a) Hepatic artery carries deoxygenated blood to the intestine. 72-8 19 [P.7.0.67. 68, 69, (72-8, ‘The potential difference across the membrane of nerve fibre, when it does not show any physiological activity, is called resting potential, which is about (@) -60 mv () -80 mv () +60 mv (4) +80 mM In the following ozonolysis, the product is O3/H202 (a) maleic acid () pyruvic acid (6) succinic acid (a) None of the above Order of the electrophilic substitution in the following compounds will be Me a Me +NHy i ii iii iv (a) i> ii> i> iv (0) iti>ii>ioiv () ii>i>iiriv (@) iv > iii> ii>j 2070. 71. 72. 73. 74. 172-8 Dysfunction of cyclin-dependent kinase 5 (Cdk) has been implicated in a number of disease conditions, except (a) Alzheimer's disease (b) Amyotrophic lateral sclerosis (c]_ Niemann-Pick type C disease {@) Tay-Sachs disease Animal caps isolated from the embryo during gastrulation will develop into (a) neural tissue (b) lung tissue (0) skin (@) intestinal tissue Which of the following is an antiapoptotic protein? (a) BAD (b) Bel-2 (c) Bax (@) All of the above Jn situ end labeling (ISEL) is a technique to measure (@)_necfosis (b) apoptosis (0) cell growth (@) cell differentiation During development, which of the following phases of cell cycle increases progressively? (@) G1 phase (0) phase (0) G2 phase (4) M phase - 21 [P.T.0.75. Treatment of cells with cytochalasin D will result in (a) inhibition of actin polymerization (b) inhibition of microtubule polymerization (c)_ inhibition of intermediate filament polymerization (@) None of the above 76. A protein in the laboratory is a cystein-dependent aspartate-specific protease. It will most likely function as a/an (@) endonuclease (b) exonuclease (c) kinase (@) caspase ‘77. A mutation that causes complete loss of gene function is called (a) amorphic (6) hypomorphic (©) hypermorphic (@) neomorphic 78. An AT-rich DNA fragment with mel approximately iting temperature (T,) of 60°C will contain (@) 16 GC pairs and 6 AT pairs (0) 6 GC pairs and 21 AT pairs (9) 8 GC pairs and 19 AT pairs (4) 15 GC pairs and 15 AT pairs (72-4 279. 80, 81. 82. (72-A Prospective husband and wife should know their Rh factors because the situation can be serious due to biological incompatibility in which one of the following cases? (a) Rh* husband and Rh* wife (b) Rh husband and Rh” wife ()_ Rhohusband and Rh* wife (4) Rh* husband and Rh wife In Ca?* homeostasis, the hormone counteracting calcitonin action is (a) glucagon (>) thyroxine ()_ insulin (4) parathyroid hormone ‘The rate of DNA synthesis by standard DNA polymerase is 1-2 kb per minute. A pathogen replicates its ~25 Mb genomic DNA in 10 hours inside the host. This is possible due to (a) manifold enhancement of rate of DNA synthesis by DNA polymerase (0) use of multiple origin of replication initiation sites (c)_ DNA replication is coupled to transcription that facilitates faster DNA replication rate (@) pathogen borrowing host DNA polymerase that also helps in faster DNA synthesis, Which of the following statements regarding the polymerase chain reaction is not true? {a) It can increase the amount of DNA in a sample (b) It has the potential of diagnosing an infection from a single copy of a gene (c) It utilizes DNA polymerases from psychrophilic organisms (@) It can amplify DNA of only a few base pairs up to a whole genome 23 [P.7.0.83, 84, 172-4 CpG islands and codon bias are the tools used in eukaryotic genomics to (@)_ identify open reading frames (b) differentiate between eukaryotic and prokaryotic DNA sequences (c) find regulatory sequences (@) look for DNA-binding domains Large numbers of different antibodies are produced from fewer than 300 major genes by (a) gene duplication (b) alternative splicing mechanisms (c) the formation of nonspecific B cells (4) recombination, deletion, and random assortment of DNA segments Shotgun cloning differs from the clone-by-clone method in which of the following ways? {@) The location of the clone being sequenced is known relative to other clones within the genomic library in shotgun cloning (®) Genetic markers are used to identify clones in shotgun cloning (©) Computer software assembles the clones in the clone-by-clone method (a) i map of the genome is needed to begin shotgun cloning (a) BLAST analysis of the protein sequence ®) BLAST analysis of the DNA from the coding sequence (©) BLAST analysis of the DNA from the upstream regions (4) None of the above 2487. 90. 172-8 Secondary structure of RNA molecules {a} depends on complementary base pairing (©) is generated by covalent bonding between sections of the RNA molecule (¢) can be described as interactions between portions of the backbone of the molecule (a) does not have an impact on the function of the molecule Which of the following types of symmetry are shown by majority of virus particles? (a) Complex and helical (0) Cuboid and helical (o) Bilateral and helical (@) Complex and bilateral Which of the following can be described as a sequence that can be several thousand base pairs upstream or downstream of an eukaryotic promoter and which increases gene expression as much as 200 fold? (a) CAAT box (0) TATA box (c) Insulator (@) Enhancer In which of the following situations would cells die by necrosis but not by apoptosis? (2) Removal of virus-infected cells (b} Removal of developing neurons which fail to make profitable connections with other cells (c) Removal of heart muscle cells damaged by oxygen depletion following cardiac infarction (a) Removal of cells with damaged DNA which cannot be repaired kek 25 [P.T.0.SPACE FOR ROUGH WORK 72 172-A eaSPACE FOR ROUGH WORK -—900x2 72-4 27 E15.