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# WEEK 3: Non-Parametric Tests

## What is a non-parametric test ?

Methods of analysis that do not assume a particular family of distributions for the
data.

## Non-parametrics are distribution free;

The data may not be put into metric form appropriately, i.e.. unordered
qualitative variables (Nominal data);
Data may be rank ordered (Ordinal data);
Data may be from small samples;
There may be non-normal distribution of the variables (Skewed data);
Outliers may be present

## Non-parametric v Parametric tests

Usually only perform one analysis of a data set choosing between parametric
and non-parametric methods.

## It is usual to use a parametric method, unless there is a clear indication that it

is not valid.

It is important to realise that if we apply different tests to the same data then
we do not expect them to give the same answer, but in general two valid

Non-parametric tests are less powerful than the equivalent parametric test
(especially in small samples) and will tend to give a less significant (larger) p-
value

## Dealing with ordinal data

Non-parametric tests are usually based on Order Statistics and Ranks.

## RANKS : their places in this order.

Exercise in ranking data

data: 7 9 10 12 12 9 12 11 -13
X1 X2 X3 X4 X5 X6 X7 X8 X9

ordered:

ranks:
Wilcoxon Signed Rank Test

## Non-parametric equivalent for testing or estimating location for a one sample

problem (equivalent to one sample t-test) OR for paired samples (equivalent to
the paired t-test)

Assumptions

## A random sample of n independent observations OR independent random

pairs are taken.
The variable of interest is the difference (d) :
For the one sample problem d = Observed value - Hypothesised value
For paired data d = X - Y - where X is value at time 1 and Y is value at
time 2.
The level of measurement is at least ordinal.

## Example 1 - Two Groups of Paired Data

Non-parametric equivalent of paired sample t-test

## Acne severity scores compared at baseline and 4 weeks following a course

of erythromycin 500mg twice daily.

## Subject Baseline Week 4 Difference

(Week 4-
Baseline)
1 12 8
2 11 5
3 6 4
4 4.5 1
5 5.5 5
6 7 6
7 3.5 3
8 8 8
9 9 8.5
10 10 6

Question 1:
1. Calculate the difference between week 4 and baseline
2. Rank the differences (If there is tied ranks (=0) ignore this and start from
the first number).
3. Calculate the sum of ranks for ve and +differences.
4. Open the data file: Paired Wilcoxon
5. Use a Wilcoxon test the hypothesis that there been a change in severity
scores from baseline to week 4?
6. Undertake a the appropriate t-test and compare the findings.

## Example 2 - One independent sample

The average daily energy intake (kJ) over 10 days of 11 healthy women is shown
in the table below. Open the data file: One sample wilcoxon

## Subject Daily energy Difference from Ranks of

intake (kJ) 7725 kJ differences
1 5260
2 5470
3 5640
4 6180
5 6390
6 6515
7 6805
8 7515
9 7515
10 8230
11 8770

Question 2:

## (NOTE: SPSS treats the data as paired)

1. Calculate the difference between enery intake and the test valve
2. Rank the absolute differences
3. Calculate the sum of ranks for ve and +differences.
4. Open the data file: Paired Wilcoxon. What can be said about the
energy intake compared to the recommended daily
intake of 7725 kJ, using the Wilcoxon test the hypothesis.
5. Undertake a the appropriate t-test and compare the findings.
Friedman Test

## The assumption that the residuals have a Normal distribution cannot be

assessed before fitting the model. Sometimes, however, it can be seen from the
raw data that the model will not fit well. In particular, wide variation in the
standard deviations for each row and column will suggest problems with the
parametric two-way ANOVA.

Therefore, we have a non-parametric equivalent of the two way ANOVA that can
be used for data sets which do not fulfill the assumptions of the parametric
method. The method, which is sometimes known as Friedmans two way
analysis of variance, is purely a hypothesis test.

Example 3
Acne severity scores compared at baseline and 4 weeks following a course
of erythromycin 500mg twice daily.

## Subjects Week 0 Week 4 Week 8 Week 12

1 12 8 6 5
2 11 5 5 6
3 6 4 4 5
4 4.5 1 2 1
5 5.5 5 5 5
6 7 6 6 5.5
7 3.5 3 3 3
8 8 8 7 5
9 9 8.5 7 4
10 10 8 6 4
11 12 8 6 5
12 12 7 5 4
Mean 8.375 5.958 5.167 4.375
SD 3.076 2.398 1.528 1.334

Question 3:
1. Open data file: Friedman
2. Is there any variation in acne severity scores over the
12 week period?
3. If the Friedman test shows significance, then undertake
paired tests to see where the differences are.

## Non-parametric equivalent of two sample t-test.

The Mann-Whitney test is used to compare two sets of data from independent
groups. It is the most commonly used alternative to the independent samples t-
test. The values from both samples are combined and then the data is ranked
from smallest to largest. The rank of 1 is assigned to the smallest value, 2 to the
next smallest and so on. If the ranks are tied, then the average rank is used.

Assumptions
1. There are two independent random variables (X and Y), of size n and m.
2. The variable of interest is a continuous random variable.
3. The two populations differ only with respect to location.
Example 4
Patients receiving chemotherapy as outpatients were randomized to receive
either an active antiemetic treatment or placebo (Williams et al., 1989). The
following table shows measurements (in mm) on a 100mm linear analogue self
assessment scale for nausea.

## Active (n=20) Placebo (n=20) Rank Active Rank

Placebo
0 0
0 10
0 12
0 15
0 15
2 30
7 35
8 38
10 42
13 45
15 50
18 50
20 60
20 64
21 68
22 71
25 74
30 82
52 86
76 95
Sum of the ranks ? ?

Question 4:

## 1. Rank the data, irrespective of group.

2. Calculate the sum of the ranks for the active and placebo group
3. Open data file: Mann Whitney
4. Undertake a Mann Whitney test to determine of there are any differences
in nausea levels between the active and placebo groups? Draw a graph to
show the differences.
5. Undertake a the appropriate t-test and compare the findings.
Kruskal Wallis Test
Just as the one way analysis of variance is a more general form of the t-test,
there is a one for the non-parametric Mann-Whitney test. The Kruskal-Wallis
test is an obvious mathematical extension of the Mann-Whitney test.

Assumptions
1. There are three or more independent random variables (X 1 , X2, X3.Xn, ), of
size n1 ,n2, n3.nn
2. The variable of interest is ordinal or a continuous random variable which is
non-normal.
3. The populations differ only with respect to location.

## Example 5 Three Groups of Independent Data

(non-parametric equivalent of the one way ANOVA)

## Fentress et al (1986) reported the results of a randomized comparison of three

groups of six children suffering from frequent and severe migraine. The active
treatments given were relaxation response, either with or without biofeedback,
and a third group of children was not treated. The frequency and duration of
headaches were recorded before and after the study period, and the difference
between these measurements was used as a measure of weekly headache
activity. Reduction in weekly headache activity for three treatment groups,
expressed as a percentage of baseline data (Fentress et al., 1986). Ranks are
shown in brackets.

## Relaxation response Relaxation Untreated

and biofeedback response alone (n=6)
(n=6) (n=6)
62 (11) 69 (10) 50 (12)
74 (8.5) 43 (13) -120 (17)
86 (7) 100 (2) 100 (2)
74 (8.5) 94 (5) -288 (18)
91 (6) 100 (2) 4 (15)
37 (14) 98 (4) -76 (16)
Rank sum 55 36 80

## Reduction in headache activity for each child is expressed as a percentage.

Note that a negative value indicates an increase in headache activity. Three
children had a complete absence of headaches at the end of the study and thus
a reduction of 100%.

Question 5:
1. Rank the data, irrespective of group.
2. Calculate the sum of ranks for each group. What does this indicate?
3. Is there variation in the reduction of headache activity between the 3
groups using a KW test? Draw a graph to show the differences.
4. Compare the findings with ANOVA.

## Number of groups of observations

Independent or dependent groups of observations
The type of data
The distribution of data
The objective of the analysis

Choice of Test

## Parametric Test Data Non-Parametric Test Data

One sample T-test Interval Wilcoxon Sign Rank Ordinal /
Test Interval(skewed)
Two sample T-test Interval Mann-Whitney U Test Ordinal /
Interval(skewed)
Paired T-test Interval Wilcoxon Sign Rank Ordinal /
Test Interval(skewed)
One-Way ANOVA Interval Kruskall Wallis Test Ordinal /
Interval(skewed)
Two-Way ANOVA Interval Friedman Test Ordinal /
Interva(skewed)l