Description

Designation:VPI5392[NCIB10635]
DepositedName:ClostridiumtyrobutyricumvanBeynumandPette
ProductSheet ProductDescription:Typestrain.

Clostridiumtyrobutyricum Propagation
(ATCC25755)
Medium
ATCCMedium2107:ModifiedReinforcedClostridialAgar/BrothMedium(prereduced)
ATCCMedium260:Trypticasesoyagar/brothwithdefibrinatedsheepblood
PleasereadthisFIRST
GrowthConditions
StorageTemp. Temperature:37C
Frozen:80Cor Atmosphere:Anaerobicgasmixture,80%N210%CO210%H2

colder PropagationProcedure

FreezeDried:2C 1. Openvialaccordingtoenclosedinstructionsorvisitwww.atcc.orgforinstructions.
to8C 2. Underanaerobicconditionsasepticallyrehydratetheentirepelletwithapproximately0.5mLof#2107
LiveCulture:See broth.Asepticallytransfertheentirecontentstoa56mLtubeof#2107broth.Additionaltesttubes
Propagation canbeinoculatedbytransferring0.5mLoftheprimarybrothtubetothesesecondarybrothtubes.
Bestpracticedictatestheuseofprereducedmedia.
Section
3. Useseveraldropsoftheprimarybrothtubetoinoculatea#260plateand/or#260agarslant.
4. Incubateinananaerobicatmosphereat37Cfor48hours.Incubateoneagarplateaerobicallyat37C
tocheckforcontamination.

BiosafetyLevel
ANAEROBICCONDITIONS:
1
Anaerobicconditionsfortransfermaybeobtainedbytheuseofananaerobicgaschamberorplacementof
testtubesunderagassingcannulasystemconnectedtoanaerobicgas.

IntendedUse Anaerobicconditionsforincubationmaybeobtainedbyanyofthefollowing:
Loosescrewcapsontesttubesinananaerobicchamber
Thisproductisintendedforresearchuseonly.Itisnot Loosescrewcapsontesttubesinanactivatedanaerobicgaspackjar
intendedforanyanimalorhumantherapeuticor Useofsterilebutylrubberstoppersontesttubessothatananaerobicgasheadspaceisretained
diagnosticuse.

CitationofStrain Notes

Ifuseofthiscultureresultsinascientificpublication,it Alwaysusefreshlypreparedprereducedmediaorprereducedmediathathasbeenpreviouslypreparedbut
shouldbecitedinthatmanuscriptinthefollowing storedunderanaerobicconditions.
manner:Clostridiumtyrobutyricum(ATCC25755)
AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org

References

Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.

BiosafetyLevel:1

Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
forHealth.

ATCCWarranty

ATCCproductsarewarrantedfor30daysfromthedateofshipment,andthiswarrantyisvalidonlyifthe
AmericanTypeCultureCollection
POBox1549 productisstoredandhandledaccordingtotheinformationincludedonthisproductinformationsheet.Ifthe
Manassas,VA20108USA ATCCproductisalivingcellormicroorganism,ATCCliststhemediaformulationthathasbeenfoundtobe
www.atcc.org effectiveforthisproduct.Whileother,unspecifiedmediamayalsoproducesatisfactoryresults,achangein
mediaortheabsenceofanadditivefromtheATCCrecommendedmediamayaffectrecovery,growthand/or
800.638.6597or703.365.2700
functionofthisproduct.Ifanalternativemediumformulationisused,theATCCwarrantyforviabilityisno
Fax:703.365.2750
Email:Tech@atcc.org longervalid.

Orcontactyourlocaldistributor
Disclaimers

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 Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproductsheet,
ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureand
patentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeen
confirmedtobeaccurate.
ProductSheet Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.ATCC
isnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.Whilereasonableeffort
Clostridiumtyrobutyricum ismadetoinsureauthenticityandreliabilityofmaterialsondeposit,ATCCisnotliablefordamagesarisingfrom

(ATCC25755) themisidentificationormisrepresentationofsuchmaterials.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org

PleasereadthisFIRST AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2016.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[08/02]
StorageTemp.
Frozen:80Cor
colder

FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section

BiosafetyLevel
1

IntendedUse

Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain

Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Clostridiumtyrobutyricum(ATCC25755)

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org

800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

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