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This experiment aims to determine the reaction equilibrium constant for the given reaction and to
compare the degree of ion exchange achieved by batch-wise contact and continuous contact in a fixed bed
unit.
Theoretical Background
Anion exchange chromatography is a form of ion exchange chromatography (IEX) where separation
of molecules is done based on surface charge difference. It uses a positively charged ion exchange resin
with an affinity for molecules that has negative surface charges. Anion exchange chromatography has a
stationary bed that has an ionically positive charged surface and a negatively charge sample ions. If the
sample is more negative, the stronger it will be attracted to the stationary phase, thus, it will elute longer.
Ion exchange solids are usually synthetic resins or polymers that can either have a positive or
negative charge. These resins contains sulfonic, carboxylic, or phenolic groups that can exchange cations.
(Equation 1)
(Equation 2)
R represents the solid resin that can be used to exchange with H + or other cations or with OH- or an ions
in a solution. Anion or cation exchanger, binding capacity, and particle size of the resin are some of the
Where the total ionic group concentration [R] in the resin is constant
Thus,
Ion exchange is widely used for the separation and purification of charged biomolecules due to
its widely applicability, high capacity and simplicity, and high resolution. Other uses of it are in separation
and purification of blood components, quality control and process monitoring, food and clinical
a. Graph
Constant resin
Chart Title
0.00012
conc'n KMnO4 before reaction
0.0001
0.00008
0.00006
0.00004
0.00002
0
96 96.5 97 97.5 98 98.5 99
% T after reaction
Constant concentration
0.000012
0.000008
0.000006
0.000004
0.000002
0
96.5 97 97.5 98 98.5 99
% T after reaction
25
20
mass of resin
15
10
100
90
80
70
% T before
60
50
40
30
20
10
0
0 0.2 0.4 0.6 0.8 1
conc'n after
18
16
14
12
residence time
10
8
6
4
2
0
0 20 40 60 80 100 120
% T after
18
16
14
12
residnece time
10
8
6
4
2
0
0 0.2 0.4 0.6 0.8 1
conc'n after
b. References: http//www.news-medical.net