BIO20 Introduction to Biomimetics Engg.

and Component Design

PROBLEM SET 2: CENTRAL DOGMA OF MOLECULAR GENETICS

and RECOMBINANT DNA TECHNOLOGY

Name/s:__________________________ Signature/s:_________________ Date submitted: 30

score
__________________________ _________________ Section:
__________________________ _________________
__________________________ _________________

Direction: Answer completely the following questions.

I. Given the DNA sequence: 5 ATG CAT CGC GAC ATA CAG ACC AAA TTT TGA 3

a. Give its complementary DNA sequence.

b. Give the product when it undergoes transcription.

c. Give the product of translation of the transcribed sequence.

II. Given the Normal DNA Sequence: 5 ATG CAT CGC GAC ATA CAG ACC AAA TTT TGA 3

Determine what type of mutation occurred in the following mutant DNA.

Mutant I: 5 ATG CAT CGC GAC ATA CAG AGC AAA TTT TGA 3

a. At what codon number did mutation occurred? ___________________

b. Identify the type of substitution point mutation happened. ____________________________

c. What is the resulting amino acid of the codon that was mutated?

d. What is the consequence of that mutation?

Mutant II: 5 ATG CAT CGC GAC ATA TAG ACC AAA TTT TGA 3

a. At what codon number did mutation occurred? ___________________

b. Identify the type of substitution point mutation happened. _____________________________

c. What is the resulting amino acid of the codon that was mutated?

d. What is the consequence of that mutation?

III. Direction: Answer completely the following questions.
1. Enumerate the general steps in
Recombinant DNA Technology. (5pts)
4. DNA is isolated from a hair sample taken
from a comb dropped at the scene of a bank
robbery. A DNA fingerprint is derived from the
sample (labelled H) and from 7 suspects
(numbered). Using the results from a multi-locus
probe shown below, what conclusions would
you like to make? To whom is the hair most
likely to belong? (4pts)

2. What is polymerase chain reaction (PCR)?

Enumerate the general steps in PCR. (4pts)

3. Describe the following techniques and what

do they function for (4pts)
a) Gel Electrophoresis
b) Southern Blot Hybridization

Answer:

utasevilla@mapua.edu.ph INTRODUCTION TO BIOMIMETICS ENGINEERING

AND COMPONENT DESIGN 2