Beruflich Dokumente
Kultur Dokumente
RAG1-FW GAGATGTGAAGTCCGTGTGC
RAG1-RV CGTTCCAGGTTCTCAGCATG
NEK1-FW AGGCAAGAAAAGGAAAGGGT
NEK1-RV TGTGGTAGGGAATGGATGGT
G6PD-FW GCAAGACACTCTCTCCCTCA
G6PD-RV AGCTGAGGAATGTAGCTGGG
HBB-FW GCTGGGCATAAAAGTCAGGG
HBB-RV TCCTGAGACTTCCACACTGA
SRY-FW CGAACTCTGGCACCTTTCAA
SRY-RV GTTACCCGATTGTCCTACAGCT
TACGCCAGGCCTCACGGAGCTCGTCGCTGAGGGGACATAGTATGA
ssODN1
AGCTTGGGAGGCCGGTGGCACACAGGGAGGGAGGGCAAAGGCC
(G6PD)
AC
GGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCA
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAA
ssODN2
AGCTTTCAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGCAGC
(HBB)
CTAAGGGTGGGAAAATAGACCAATAGGCAGAGAGAGTCAGTGCC
TATCAGAAACCCAAGAGTCTTC
ssODN3 CCTTAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGA
(HBB) AAGCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCC
ssODN4 CCTCCCAAGCCATACTATGTCCCCTCAGCGACGAACTACGCGAAG
(G6PD) CTTGGCGTATTTTCACCCCACTGCTGCACCAGATTGAGCTGGAGA
Figure S1: Off-target analysis in G6PD editing embryo #2. a. Sequence of selected top 13
potential off-target sites. b. T7E1 digestion of PCR amplified off-target site region. * indicates T7E1
assay positive samples. c. Sequencing results of OT10OT11 and OT12 PCR samples.