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Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts? (a) Laboratory flask of largest capacity. : (b) A stirred-tank bioreactor without inlets and : outlets. (2) A continuous culture system. (d) All of the above. i The role of DNA ligase in the construction of a : recombinant DNA molecule is : (a) formation of phosphodiester bond between two : DNA fragments H (b) formation of hydrogen bonds between sticky ends of DNA fragments : (0) ligation of all purine and pyrimidine bases (d) none of the above, i Which of the following sequences is recognized by restriction enzyme Bam |? ! 1 | "— GAATIC- 3° (yy 5! ~AAGCTA- 3° : — CTTAAG - 5° z aoe Ly r tT (a) 2 4 (a) 5 ~ CCCAAT ~ 3 4 @ 5’ — GGATCC - 3" v- aoe i z- Ca a Which of the following is not a cloning vector? (a) Cosmid (b) pBR322 (0 Sall (d) Phagemid The correct sequence of making a cell competent is (a) treatment with divalent cations —> incubation of cells with recombinant DNA on ice — heat shock ! (42°C) — placing them back on ice. heat shock (42°C) — incubation of cells with recombinant DNA on ice — treatment with : divalent cations — placing them back on ice. ! (b) (treatment with divalent cations placing cells ‘on ice —> incubation of cells with recombinant DNA on ice — heat shock (42°C). incubation of cells with recombinant DNA on ice —> heat shock (42°C) — treatment with divalent cations —> placing them back on ice. Identify A,B, Cand Din the given figure of E. coliconing () "vector pBR 322 and select the correct option. Cla Hin d iil (a) Hind| EcoR| amp® ori (b) Hind! = BamH\ —_ kan® amp* () BamHI Psti ori amp* (4) EcoR| BamHI amp* ori If a person obtains transformants by inserting a tecombinant DNA within the coding sequence of enzyme B-galactosidase, he will separate out recombinants from non-recombinants by which of the following observations? (2) Non-recombinant colonies do not produce any colour whereas recombinants give blue coloured colonies. Recombinant colonies do not produce any colour whereas non-recombinants give blue coloured colonies. i Recombinants and non-recombinants both produce blue coloured colonies. No colonies are formed due to insertional inact- vation. (b) ie (() 8. Which of the following infection(s) can be diagnosed | bby the use of polymerase chain reaction (PCR)? (a) HIV-1 and HIV-2 viruses (b) Hepatitis-B virus (Co) ium tuberculosis i (d) All of the above t (a) Thermococcus litoralis (b) Thermus aquaticus (9 E.coli (@) Salmonella typhimurium. 10. Match the following columns. Column-1 Column-ti A Isolation of genetic 1, ‘Separation and material Purification of the product i 8. Fragmentation of DNA 2. Recombinant protein C. Isolation of desired 3. Transformation DNA fragment D. Amplification of gene of 4. DNA ligase interest E. Ligation of the DNA 5. Polymerase chain fragment into a vector reaction F. Insertion of recombinant 6. Agarose gel DNA into the host electrophoresis G. Extraction of desired 7. Restriction enzymes gene product : H. Downstream processing 8. Ethanol A BC DEF GH @5 4 3 2 1 6 8 7 8 7 6 5 4 3 2 4 7 8 5 6 43 12 @1 2 3 5 4 6 87 11. Electroporation involves (2) promotion of seed germination by induced imbibition of water with electric current (b) making transient pores in cell membrane to : facilitate entry of gene constructs : (0. purification of saline water with the help of an ! artificial membrane i (d) passage of sucrose through sieve pores by : electroosmosis. 12, Main objective of production/use of herbicide resistant: GM crops is to (@) encourage eco-friendly herbicides (b) reduce herbicide accumulation in food articles for health safety i (0) eliminate weeds from the field without the use of : manual labour (d) eliminate weeds from the field without the use of herbicides. 13, Plasmid used to construct the first recombinant DNA ‘was isolated from which bacterium species? (a) Escherichia coli (b) Salmonella typhimurium (0) Agrobacterium tumefaciens (a) Thermus aquaticus Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNAase I | (b) restriction endonucleases purified from bacteria can be used in vitro (0) the phenomenon of transduction in bacteria is well understood (d)_ we can see DNA by electron microscope. Variable number of tandem repeats (VNTR) in the DNA molecule are highly useful in (2) recombinant DNA technology (b) DNA finger printing (6) monoclonal antibody production (@) stem cell culture. . The restriction enzymes are used in genetic engineering, because they ° (2) can degrade harmful proteins (b) can cut DNA at specific base sequence (©. canjoin different DNA fragments (d)_ are nucleases that cut DNA at variable sites. Who discovered recombinant DNA (rDNA) technology? (2) Har Gobind Khurana (b) James D Watson (Q) Stanley Cohen and Herbert Boyer (@) Walter Sutton and Avery AA student performed the following steps in a Southern blot experiment to determine the number of copies of a particular gene that has been inserted in a genetically modified organism, {i) Transfer of DNA to nitrocellulose membrane. (i) Restriction digestion of genomic DNA, (i) Cleaved DNA separated using gel electrophoresis. (iv) Create radioactive probe. (v)_ Incubate probe and membrane. Which is the correct sequence to the above steps? (2) (id Gil) > () > i) > (6) (il) > ill) > () > (W) > (iv) WW) Gi) > ii) @ WW i) > G) 14