1.

Give an example of ligase enzyme

DNA ligase

Alanine tRNA ligase

2. In a Lock and Key model of enzyme action, the part of the enzyme that
recognizes the substrate is known as

a. Enzyme-substrate complex b. Product-substrate complex

c. Active site d. Co-enzyme

3. State TRUE or FALSE

a. Lock and Key model is more simplistic compared to the induced fit
model. TRUE

b. The active site shape is complementary to the substrate in both models.

FALSE

c. Transition state stabilization occurs only in the Lock and Key model.
FALSE

d. Some enzymes Do NOT follow either model.

TRUE
4. The curve which shows competitive inhibitor, the line `A' belongs to
uninhibited enzyme kinetics (symbols has their usual meaning) [Choose
correct answer]

a. B b. C c. D d. None
 Lineweaver-Burke plots - Summary

5. Pepsin, a digestive enzyme which is secreted in stomach, and acts best

at pH ______

a. 10 b. 7 c. 6 d. 2

6.GivenGcat = 25 kJ/mol, Guncat = 65 kJ/mol, R = 8.413 JK-1mol-1 and

room temperature condition, calculate the rate enhancement by this
enzyme.

Ans. Kuncat = e-65000/8.413*298= 5.498* 10-12

Kcat = e-25000/8.413*298= 4.670* 10-5

Rate enhancement = Kcat/Kuncat= 8.494*106~ approx. 8.5 * 106

 Vmax S
v
Use Michaelis-menten eqtn o K S
M
Substitute v=Vmax/4 , solve [S] as we know Km , kcat is given to confuse us

Double reciprocal plot

7. Write down the Michaelis-Menten equation.

Using this equation determine the reaction rate when the substrate
concentration [S] is equal to
i) saturation condition i.e. [S] = infinity
ii) Km
iii) Km/2
iv) 2Km
v) 10Km

Vmax S
vo
Answer:-
K M S

I. Rate of rxn = Vmax

II. Rate of rxn =

III. Rate of rxn=

IV. Rate of rxn =

V. Rate of rxn =

8. State three characteristic features of an enzyme

They are basically the folded proteins hence their work is affected by temperature
and pH

Enzymes Speed up chemical reactions by lowering the activation energy

Enzymes alter the rate of a chemical reaction without undergoing a permanent

change in structure

Small amount of enzyme can affect the change of large amount of substrate