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IS 7250 : 1995
(Reaffirmed 2003)
(mJ*-m)
Indian Standard
METHODOFTESTFOREVALUATION
OF INSECTPROOFNESSOFWOOLi,EN
TEXTILES
(First Revision)
ICS 59.060.10
0 BIS 1995
FOREWORD
This Indian Standard (First Revision) was adopted by the Bureau of Indian Standards, after the draft
finalized by the Chemical Methods of Test Sectional Committee had been approved by the Textile
Division Council.
Woollen fabrics and garments are frequently damaged by larvae of dermested beetle and moths and result
in enormous economic loss. Among beetles, the woolly bear (Anthrenusflavi~~~Lec) and the carpet beetle
(Attagenus fascia&s); and among moths (Tinea pellionella Linn and Tinea translucens) and the webbing
clothes moth (Tineola bisselliella Hum) aremainly responsible for damage throughout India.
The standard originally published in 1974 provided use of larvae ofAnthrenusflavipes and Tineapellionella.
species. The standard has been revised to incorporate use of additional insect. Details for breeding of
larvae of test species have also been included.
The earlier version of the standard specified temperature of 30 + 0.5C and 75 -I 2 percent relative
humidity. The revised standard adopts standard atmospheric conditions for textiles, namely, 27 + 2C
temperature.and 65 +- 2 percent relative humidity.
In the preparation of this standard, assistance has been drawn from IS0 3998 : 1977 Textiles -
Determination of resistance to certain insect pests.
In reporting the results of a test or analysis made in accordance with this standard, if the final value,
obscrvcd or calculated, is to be rounded off, it shall be done id accordance with IS 2 : 1960 Rules for
rounding off numerical values (revised).
IS 7250 : 1995
Indian Standard
METHODOFTESTFOREVALUATION
OF INSECTPROOFNESSOFWOOLLEN
TEXTILES
(First Revision)
3.3 Weighing bottles with stopper. Yarn 200 mg specimens, wound into a loose
hank in the container
3.4 -Balance capable of determining mass to an
accuracy of 0.1 mg. 6 TEST INSECTS
3.5 Die of 402 1.5 mm diameter for punching cir- 6.1 The larva of any of the following test insects
cular test specimen. shall be used. Choice of test species depends-upon
4 CONI)IHONING, RF,4RING AND TESTING the species against which proofness is required.
ATMOCPI-IFRE
L J Anthrenus jlpipes and Attagenus fascia&s are
difficult to control and thus require high level of
The atmospheric conditions for rearing and testing insectproofing agent than Tinea translucens:
shall have 27 ? 1C temperature and 65 + 2 percent (i) Anthrenus jlavipes lo-11 weeks old
relative humidity.
(ii) Attagenus fasciatus 11-12 weeks old
5 SPECIMENS (iii) Tinea translucens ,24-27 days old
5.1 Test Specimens 6.2 Details of breeding of the above larvae are
Eight test specimens shall be selected at random given in Annex A. In case, there is any deviation in
Irom the sample of material to be tested. Use four rearing medium or temperature and relative
1
1s 7250 : 19%
humidity, it may call for some deviation in the age m2 = mean initial mass of appropriate
of the larvae used for tests. In that case the devia- moisture regain controls, and
tion shall be indicated in the test report. Differen- m3 = mean final mass of appropriate mois-
ces caused by variation in temperature and ture regain controls.
humidity are largely overcome by use of Standard
conditions given in 4. 8.2 Visual Assessment of Insectproofness
7.5 Count and record for each test specimen the Count and record for each test specimen the num-
number of surviving, dead or pupating larvae. ber of larvae in each of the following conditions:
a) Live,
7.6 Transfer the test specimen, thevoracity control
specimens and moisture regain control specimens b) Dead, and
to small tared weighing bottles. Determine the c) Pupating.
mass separately of the test, voracity control and
moisture regain control specimens. 8.4 Assessment of Insectproofness
7.7 Test is invalid if the mean loss in mass of the 8.4.1 A tested sample of fabric, carpet or yarn shall
four voracity controls is Jess than 35 mg, or any be considered a borderline case of satisfactory in-
single value is less than 2.5 mg or more than 25 sectproofncss if any of the following applies:
percent of the control larvae die or pupate.
a>Visible surface damage and estimation of
holes is assessed as attack level 2 B on two
8 OHSERVATIONS AND CALCULATIONS of the test specimens and the remaining test
specimens are undamaged.
8.1 Method of Calculation and Formula
b) Visible surface damage and estimation of
8.1.1 Determine the loss in mass (no) of each test holes is assessed as attack level 3 B on any
specimen and voracity control specimen due to one test specimen with the yarn or fibres
insect feeding as follows: partially severed at more than 011e point
mo x mg (indicative of uneven application) with the
m= -ml remaining test specimens are undamaged.
m2
4 No surface damage is visible to the naked
where
eyes but the mean loss in mass is greater
1120 = mass of the test specimen or voracity than 15.0 mg or the loss in mass of any one
control specimen before exposure to test specimen is not-greater than 20 mg.
larvae,
ml = mass of the test specimen or voracity ~8.4.2 A tested sample of fabric, carpet or yarn shall
control specimen after exposure to be considered satisfactorily proofed if the attack
larvae for 14 days,
2
IS 7250 : 1995
level is assessed as less than that defined as border- b) Test insect species used;
line under 8.4.1. c) Larval conditions at the end of the test;
8.4.3 If a tested sample of fabric, carpet or yarn has d) Mean loss in mass, in mg, of the four test
level greater than that defined as borderline specimens;
under 8.4.1 then it shall beconsidered inadequately e) Assessment of visible damage;
proofed. If estimation of holes is assessed as C or
f) Mean loss in mass, in mg, of the four voracity
D on any test specimen, the sample also falls in this
control specimens;
category.
9 TEST REPORT g) Any deviation from the specified procedure;
and
The test report shall include the following:
h) Assessment of iQsectproofness: Proof/
a) Type of textile material under test; Borderline/Not proof.
ANNEX A
(Clause
6.2)
BREEDING OF LARVAE OF TEST SPECIES
3
IS 7250:1995
For starting culture release some adults on nated with a five percent dispersion of
the Medium No. 1 or 2 in suitable container brewers yeast in distilled water.
and fill three-fourths of it with medium. On b) For starting a culture, freshly emerged
the top of medium place a small piece of adults are caught by aspirator and intro-
wool fabrics for shelter of adults. Sieve the duced daily in a glass oviposition container
culture in medium at interval of 11 IO 12 having pieces of filter paper for harbourage
weeks. Larvae that pass through 1.25 mm ofmoths. These jars are covered with round
sieve but are retained by 1.00 mm sieve will mesh mosquito netting held in place with a
be used for the test (This should produce rubber band. Every morning, eggs are
larvae of 6 to 7mg). Add fresh medium as removed by inverting the oviposition jar in
and when required. Remove dead larvae a glass dish. Culthres with known number
and cast skins at biweekly interval. of eggs (150 to 200) are started in a rearing
container having above medium. Eggs
C) After 12th week sieve the culture and hatch out within 5 to 6 days. Each culture
release the larvae on fresh medium.
thus contains about 150 to 200 larvae which
will be used as test insects when 24 to 27 days
A-4.3Tinea translucens ( the tropical case making old. Add fresh medium as and when re-
..
clothesmoth) quired to the rearing jars. Remove excreta
at 15 days interval.
a) Medium
C> Selected larvae shall be released in a petri
All wool undyed, scoured worsted white dish for 24 h for fasting before their release
serge or barrack blanket piece, impreg- on test and voracity specimens.
4
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