JSS Scientific Abstracts Book

International Seminar & Exhibition on Phytopharmaceuticals Emerging Challenges and Opportunities, JSSCP, Ooty
Jagadguru Sri Shivarathreeshwara University

(Deemed to be University; Accredited ‘A’ Grade by NAAC)
Mysuru, Karnataka, India
International Seminar & Exhibition on

Phytopharmaceuticals
“Emerging challenges and opportunities”
Organized by
TIFAC CORE in Herbal Drugs

(DST, Govt. of India)
&
Department of Pharmacognosy & Phytopharmacy
JSS COLLEGE OF PHARMACY

(An ISO 9001 : 2015 Certified Institution)
Udhagamandalam (Ooty), The Nilgiris, Tamil Nadu, India
1
ISP-01
BIOEVALUATION OF ANTIDYSLIPIDEMIC AND ANTIOXIDANT ACTIVITY OF
BUTEA MONOSPERMA FLOWER IN RATS
Shrirao A.V.*1, Kochar N.I. 1, Chandewar A.V. 2
1. Department of Pharmacology, P. Wadhwani College of Pharmacy, Yavatmal, Maharashtra,
India.
2. Department of Pharmaceutical Chemistry, P. Wadhwani College of Pharmacy, Yavatmal,
Maharashtra, India.
Abstract
Butea monosperma, traditionally used for its various properties and hence in the present study,
chloroform extract of Butea monosperma flowers has been screened for its hypolipidemic and
antioxidant activity. Hypolipidemic activity was screened by inducing hyperlipidemia by Triton
WR1339 in Wistar albino rats and serum levels of lipid and oxidative stress parameters were
determined. Atherogenic index shows the measure of the atherogenic potential of the drugs.
Chloroform extract showed significant (p < 0.05) hypolipidemic effect by lowering the serum
levels of lipid parameters such as reduction in the level of serum cholesterol, triglyceride, LDL,
VLDL and increase in HDL level which was comparable to the standard drug Atorvastatin.
Chloroform extract exhibited significant atherogenic index and percentage protection against
hyperlipidemia. Also Chloroform extract significantly (p˂0.01) reduced oxidative stress and
normalizes the activities of SOD, CAT, GPx and GRh compared to Triton-treated rats. These
biochemical observations were further confirmed by histopathological examinations of aorta,
liver and kidney sections and are comparable with the Atorvastatin. Preliminary phytochemical
analysis revealed the presence of phytoconstituents such as steroids, flavonoids, glycosides,
alkaloids, phenolic compounds which was further confirmed by the thin layer chromatography,
High Performance Thin Layer Chromatography (HPTLC). The overall experimental results
suggests that the biologically active phytoconstituents such as steroids, flavonoids, glycosides,
alkaloids, phenolic compounds present in the chloroform extract of Butea monosperma, may be
responsible for the significant hypolipidemic and antioxidant activity which further confirmed
the traditional use of Butea monosperma as a significant hypolipidemic agent.
Keywords: Triton WR1339, Atorvastatin, Oxidative stress, Thin layer chromatography
2
ISP-02
REVERSAL OF ANORECTIC EFFECT OF INTUPPU CHURNA –AN AYURVEDIC
FORMULATION
V. S. Kulkarni*, V. Alagarsamy, S.J Surana
MNR College of Pharmacy, Sangareddy, Hyderabad.
Abstract
Intuppu Churna is a commonly used Ayurvedic formulation for the treatment of agnimandya i.e
anorexia. As per the Ayurvedic Formulary of India, part-I the medicine consist three types of
individually powdered herbs: Terminalia chebula (Combretaceae), Piper longum (Piperaceae),
Trachyspermum roxberghianum (Umbelliferae) and one mineral-saindhav lavan (rock salt). The
chief objective of the study was to determine the antianorectic effects of these drugs individually
and to compare these effects with the effect of Intuppu. The activity of the drugs was studied
after anorexia was induced in rats by (1) physical stress arising from immobilization for 60min;
(2) intraperitoneal injection of Escherichia coli lipopolysaccharide (LPS,100 μg/kg body
weight); and (3) intraperitoneal administration of fluoxetine (8 mg/kg body weight). Similar
doses of the extracts were tested on freely feeding rats and on rats that had been deprived of food
for 20 h. Corticotrophin releasing factor (CRF, 0.3 μg/rat) can induce anxiogenic-like behavior
and reduced food intake. This model was also studied, and the results were compared. The
present study shows that Intuppu churna effectively reduces the marked anorexia induced in rats
by exposure to different stressful conditions. IF 200 and 400mg/kg significantly reversed
restraint stress, LPS, Fluoxetine and CRF induced anorexia. While the drug in the used dose
range does not affect food intake of freely feeding and non stressed food deprived rats. IF shows
the anti anorectic effect in restraint stress, LPS, Fluoxetine and CRF induced anorexia models
which indicates that the action of IF is nonspecific.
3
ISP-03
ANTHELMINTIC AND ANTI OXIDANT ACTIVITY OF KARAMARDA FRUITS

(CARISSA CARANDAS)
Rajeh bolleddu, ministry of ayush, national research institute of ayurvedic drug development 4-
cn block, sector v, salt lake city, bidhannagar, west bengal- 700091
Abstract
Karamarda- Carissa carandas is an Ayurvedic plant used forthe treatment of acidity, indigestion,
wound healing, skin diseases, urinary disorders and diabetic ulcer.Carissa carandas
(Apocynaceae)
fruits aqueous extract was investigated for anthelmintic activity using Earthworms (Pheretima
posthuma). Various concentrations (10-50 mg/ml) of aqueous extract were screened for
anthelmintic activity. Albendazole (20 mg/ml) was used as reference standard. Determination of
paralysis time and death time of the worms were recorded at each concentration. The aqueous
extract also showed good
antioxidant properties in Nitric oxide (IC50-70 µg/ml), DPPH radical scavenging activity (IC50-
850 µg/ml) in comparison with standard drugs like Ascorbic acid and Curcumine. Further its
antioxidant
activity was confirmed by Reducing power (Total anti oxidant activity) method. Increasing
concentration of aqueous fruit extract shown good reducing power. Preliminary phytochemical
screening reported the presence of carbohydrates, proteins, saponins, phenols and flavonoids.
The rich concentration of total phenols (50 mg GAE/g), flavonoids (12mg RE/g) present in
Karamarda fruits may be responsible for observed antioxidant potential.
Key words: Carissa carandas, anthelmintic activity, DPPH, reducing power, phenols.
4
ISP-04
PREPARATION AND EVALUATION OF GINGEROL PHYTOSOME LOADED

CHITOSAN COMPLEX
Singh Rudra Pratap*, Gangadharappa H. V., Mruthunjaya K.
JSS College of Pharmacy, Jagadguru Sri Shivarathreeswara University, Mysuru-570015, India.
Abstract
Gingerol (Zingiber officinale) belongs to Zingiberaceae family exhibits low bioavailability, poor
water-solubility and is rapidly eliminated from the body. The aimed to use novel delivery system
to improve the bioavailability and prolong the retention time of gingerol in the body. The
complex of GPLC containing different molar ratios (1:1:0.25, 1:1:0.50, 1:2:0.25, 1:2:0.50,
2:1:0.25, 2:1:0.50 and 2:2:0.25, 2:2:0.50) was produced by loading of gingerol-phytosomes (GP)
in chitosan solution using anti-solvent precipitation technique. GPLC and GP were characterized
and evaluated by different parameters. DSC and FT-IR demonstrated that the compatibility and
confirmation of complex of gingerol with soya lecithin and chitosan. The optimized GPLC and
GP were irregular particle shapes & spherical and oval structures, with a mean particle size of
254.01±0.05 nm, 431.21±0.90 nm and zeta potential of -17.53 mV, -13.11 mV, respectively. The
% entrapment efficiency and % drug loading of GPLC and GP was 86.02±0.18 %, 84.36±0.42 %
and 59.26±0.71%, 58.05±0.03%, respectively. The in vitro release rate of GP (86.03±0.06%) was
slower than GPLC (88.93±0.33%) in pH 7.4 phosphate buffer up to 24 h. GPLC (IC50 =17.70
µg/ml) has shown the potent antioxidant activity (Vitamin C, IC50 = 5.51 µg/ml) as compare to
GP (IC50 = 48.23 µg/ml) and comparative to Aspirin (78.62 ± 0.13 %) as a reference and GP
(65.43 ± 0.01 %), GPLC (76.54 ± 0.02 %) also shows better % inhibition on HRBC. Thus,
GPLC may be used as a sustained drug delivery system for lipophilic compounds with poor
water-solubility and low oral bioavailability.
Keywords: Gingerol phytosome loaded complex (GPLC), Entrapment efficiency (EE), Drug
loading (DL), Scanning electron microscope (SEM), Human Red Blood Cells (HRBC).
5
ISP-05
ANTI-PYRETIC ACTIVITY OF AEWA HERBAL FORMULATION IN BREWER’S

YEAST INDUCED PYREXIA IN RATS
Mu Mu Sein Myint, Khin Phyu Phyu, Khine Khine Lwin, San San Myint, Myint Myint Khine,
Nu Nu Win and Aye Aye Mon, Department of Medical Research (Myanmar)
Abstract
The aim of the present study was to investigate the anti-pyretic activity of AeWa herbal
formulation by Brewer's yeast induced pyrexia in Wistar rats. AeWa herbal formulation consists
of 13 medicinal plant parts and some of which are reputed for anti-pyretic effect. The acute oral
toxicity was carried out in albino mice according to OECD 423-guidelines. It revealed that there
is no toxic sign up to the dose level of 2000 mg/ kg body weight. Adult albino rats of either sex
(200-250 gm) were divided in to five groups containing six in each group for anti-pyretic study.
Before yeast injection, the basal rectal temperature of rat was recorded and after that, the rats
were given subcutaneous injection of 10 ml / kg of 15% yeast solution. Rectal temperature of
each rat was again measured 19 hrs after yeast injection. Then, the test drugs and standard drug
were administered orally into different groups. Three doses of test drug (1, 1.5 and 2 gm/kg body
weight) were used. Paracetamol (150 mg/ kg) was administered to standard group. Rectal
temperatures were measured at 1, 2, 3, 4 and 5 hours after test drug administration. This herbal
formulation (2 g/kg) showed significant reduction of yeast induced pyrexia in rats at 1hr, 2hr, 3hr
and 4hr after administration of test drug (p< 0.001) when compared to the control group. The
present results show that AeWa herbal formulation possesses a significant anti-pyretic effect in
Brewer’s yeast induced pyrexia in rats.
6
ISP-06
HYPOGLYCEMIC EFFECT OF TRADITIONAL MEDICINE FORMULATION
(SNG-01) ON ALLOXAN INDUCED DIABETIC MICE
Myo Nanda Aung, Khin Phyu Phyu, Khine Khine Lwin, Mu Mu Sein Myint,
Aye Win Oo, PhyoWai Zin, Department of Medical Research
Abstract
Diabetes mellitus has become important disease causing high rate of morbidity and mortality. In
this study, the objectives were to determine hypoglycemic effect of different doses of Traditional
Medicine Formulation (TMF SNG-01) on alloxan induced albino mice and to compare
hypoglycemic effect of standard drug, metformin and TMF SNG-01 and to find out the acute
toxicity. Hyperglycemia was induced by intra-peritoneal administration of 150 mg/Kg body
weight of 5% alloxan monohydrate. Mice were divided into 5 groups consisting of 6 mice.
Group 1 was given 10 ml/Kg body weight of distilled water. Group 2 to 4 were treated with TMF
SNG-01at dose of 1g/Kg, 2 g/Kg and 4 g/Kg body weight respectively. Group 5 was given 500
mg/Kg body weight of Metformin. All groups were given orally for 14 days. Blood glucose
levels were measured before administration and 1 hour interval for 4 consecutive hours. These
test procedures were done on Day 1, 7 and 14. On day 7, 4g/kg and 2g/kg body weight of TMF
SNG-01 showed significant decrease in blood glucose level at 3 hours and 4 hours when
compared with untreated diabetic group. On day 14, 4g/kg body weight of TMF SNG-01 showed
decrease in blood glucose level at 2 hours when compared with control group. In conclusion,
TMF SNG-01 possessed significant hypoglycemic effect at the dose of 2 g/kg and 4 g/kg after 7
days of treatment on alloxon induced diabetic mice. Among 3 doses, TMF SNG-01 (4 g/kg) had
the most effective hypoglycemic activity.
7
ISP-07
EFFECT OF TRADITIONAL DRUG “AEWA ” ON WOUND HEALING IN
LABORATORY RAT MODEL
Abstract
Various plant products have been used for the treatment of wounds over the years. In this study,
the effects of traditional drug lotion, AeWa, on wound healing were studied in laboratory rat
model. For in-vitro antibacterial activity, the standard method of agar disc diffusion was used.
Diameters of zone of inhibition were 13, 12 and 10 mm in disc containing AeWa for
Staphylococcus aureus, Escherichia coli and Pseudomonas species respectively, whereas 30, 30
and 25 mm in disc containing standard Ciprofloxacin (5µg) for S. aureus, E. coli and P. spp
respectively. For in-vivo test, forty five male albino rats were tested. Group I animals were kept
without treatment. Group II animals were treated with standard antiseptic (Povidone Iodine).
Group III animals were treated with AeWa lotion. Application mode was topically, once daily for
14 days. The wound was made on the inter-scapula skin surface of the rat and infected by the
bacteria described. After 48 hours, the wound area was measured daily by ruler for
progressiveness. In vitro test showed AeWa has obvious antibacterial activity on S. aureus and
then followed by E. coli and P. species. According to in vivo test, wound healing activity of
AeWa was not statistically different from wound healing activity of standard antiseptic on wound
infected with S. aureus and E. coli. However, wound healing activity of AeWa was statistically
significant than that of Povidone Iodine on wound infected with P. species.
8
ISP-09
GC-MS ANALYSIS OF AQUEOUS EXTRACT AND ANNONA MURICATA MEDIATED

SILVER NANOPARTICLES-LARVICIDAL, ANTIBACTERIAL, ANTI-OXIDANT AND
CYTOTOXIC PROPERTIES
S B Santhosha, M J N Chandrasekara,
a
Department of Pharmaceutical chemistry, JSSCP, Ooty.
Abstract
This study aims to evaluate the chemical constituents and biological potential of aqueous leaf
extract and green synthesized silver nanoparticles (AgNPs) from Annona muricata. The GC-MS
analysis of aqueous extract showed major phyto compounds Dioctyl Phthalate (21.23 %) and
Tetracosane (9.63 %). In the present study, the larvicidal potential of aqueous extract and AgNPs
synthesized from A. muricata plant leaf extract against fourth instar larvae of Anopheles
stephensi, Culex quinquefasciatus and Aedes aegypti were determined. Various concentrations of
synthesized AgNPs (10, 20, 30, 40 and 50 ppm) and the aqueous leaf extract (100, 200, 300, 400,
and 500 ppm) were tested and the maximum mortality was noticed in AgNPs than aqueous leaf
extract of A. muricata against A. stephensi, C. quinquefasciatus and A. aegypti had LC50 values
of 24 h 37.70, 31.29, 20.65, and 546.7, 516.2, 618.4 ppm, respectively. We found that the AgNPs
have better antibacterial activity against both gram-positive and gram-negative bacteria (more
than 24 mm in diameter zone of inhibition). The DPPH free radical scavenging activity is the
evident of anti-oxidant activity (70% inhibition in 100 µg/ml). In cytotoxic activity, treatment
with aqueous and AgNPs caused membrane damage and cell shrinkage in a dose dependent
manner with lower concentration of IC50 values 98.58 and 36.53 µg/ml against HeLa cell line.
The morphological changes, membrane damage, cell shrinkage and cell death were evidenced by
MTT assay, AO/EB staining and DNA fragmentation. Moreover, the AgNPs showed excellent
results than aqueous extract in all the biological activities. Based on the results obtained from the
present study, the biologically synthesized AgNPs can be utilize for the medical applications in
future.
9
ISP-10
STABILITY OF PHYTOCHEMICALS AS SOURCES OF FREE RADICLE

SCAVINGING NUTRACEUTICALS IN UNDER-UTILIZED INDIGENOUS EDIBLE
TUBERS OF VELLIANGIRI HILLS, COIMBATORE DISTRICT
Veena.B*, Anusuya devi.R and K.Thenmozhi
Department of Botany, Kongunadu Arts and Science College, Coimbatore-641029
Abstract
Recently bioactive constituents have engrossed a lot of importance as sources of functional
constituents in food product formulations. In this study, the nutritional evolution and
phytochemical composition of ethanolic extract of the under-utilized edible tubers by tribal
communities of Malasars and Irulars viz., Zeneria scabra (L.f) Sond. (Cucurbitaceae), Curculigo
orchioides Gaertn. (Amaryllidaceae), Decalepis hamiltonii Wight.& Arn. (Asclepiadaceae),
Hedychium spicatum Sm.in A.Rees (Zingiberaceae) and Cyperus stoloniferous Retz.
(Cyperaceae) were investigated. The non-enzymatic antioxidant profile revealed the presence of
phenolics, alkaloids, tannins, steroids, flavonoids, and terpinoids. The constituents of macro and
micro elements revealed that tubers contain substantial amount of potassium, calcium,
magnesium, iron, zinc and phosphorus. Among those under-utilized edible tubers examined,
Cyperus stoloniferous Retz. displayed remarkably appreciable amount of their contents.
Therefore the present investigation evinces that the under-utilized tubers can be freely explored
for their therapeutic potential and thereby justifies its nutraceutical exertion in folklore medicine
and therapy.
10
ISP-11
PROXIMATE COMPOSITION, NUTRITIVE CONTENT AND PHYTOCHEMICAL

INVESTIGATION OF WILD EDIBLE FRUITS OF VELLIANGIRI HILLS,
COIMBATORE DISTRICT
Anusuya devi.R *, Veena.B and K.Thenmozhi
Ph.D., Research scholar, Department of Botany,
Kongunadu Arts and Science College,
Coimbatore-641029
Abstract
Numerous wild edible fruits from different families are distributed in poondi, Velliangiri hills,
Coimbatore. The Malasars and Irulars Tribal people consume these fruits as natural source of
food supplement. Five wild edible fruits of Ziziphus oenoplia (L.) (Rhamnaceae), Z. jujuba Mill.
(Rhamnaceae), Limonia acidissim L. (Rutaceae) Phyllanthus emblica L. (Euphorbiaceae) and
Ficus racemosa L. (Moraceae) possesses high nutritional potential and medicinal properties of
ethanolic extracts of underutilized fruits. Qualitative and quantitative phytochemical screening
revealed the presence of phenolics, alkaloids, flavanoids, and terpenoids.The macro and micro
elements and their constituents explored that fruits of Z. jujuba Mill. , Phyllanthus emblica L.
and Ficus racemosa L. contain higher content of iron. This study revealed that these under-
utilized fruits can be exerted in the field of nutraceutical and therapy.
Key Words: Edible, Malasars, Irulars, nutritional, potential, phytochemicals.
11
ISP-12
NETWORK PHARMACOLOGY BASED APPROACH TO UNDERSTAND THE

MECHANISM OF LAGENARIA SICERARIA: A CORRELATIVE STUDY FOR THE
TREATMENT OF OBESITY
R Logesh 1*, M. Suresh Kumar 1, S.P. Dhanabal 1 and S Rajan 2.
1. Department of Pharmacognosy and Phytopharmacy, J.S.S, College of Pharmacy, Ooty-643001,

TN, India. Jagadguru Sri Shivarathreeswara University, Mysuru, India.
2. Officer In-charge, Survey of Medicinal Plants and Collection Unit, CCRH, Ministry of
AYUSH, Ooty-643209, TN, India.
Abstract
Herbal medicines are becoming more mainstream in clinical practice and show value in treating
and preventing diseases. However, due to its extreme complexity both in chemical components
and mechanisms of action, deep understanding of herbal drugs is still difficult. Thus, a
comprehensive Network pharmacological approach which could identify the active ingredients
and their potential targets and more importantly, to understand the mechanism of action for the
of herbal medicines. Experimental: In this present study, a novel system pharmacology
approaches that integrates the oral bioavailability screening, drug-likeness prediction, target,
pathway and disease identification has been established to investigate the herbal medicines.
Lagenaria siceraria was also experimentally subjected for lipase inhibitory assay and in-vivo
hyperlipidemic model. Results and discussion: The Network pharmacology approach
effectively identified 18 bioactive components from Lagenaria siceraria and 66 potential targets.
These 66 targets are closely associated with aseries of diseases of Alzheimer’s disease, Obesity,
Gout and Diabetes mellitus. These targets are further mapped to drug-target and drug-target-
pathway-disease networks to find out the target proteins. In the experimental part, the aqueous
extract (134±17 μg/mL) showed potent inhibition on lipase assay, which was less potent
compared to the Orlistat (65.6±62 μg/mL) and it also showed significant (P < 0.001) increase in
serum lipid profile such as TC, TG, VLDL and LDL. CONCLUSION: This work provides a
novel strategy for investigation of the herbal drugs containing a large number of components,
which has been demonstrated and well-studied in lagenaria. This attempt should be helpful for
understanding the definite mechanisms of action for herbal medicines and discovery of new
drugs from medicinal plants.
Keywords: Lagenaria siceraria; Obesity; Lipase assay; drug-likeness prediction; systems

pharmacology.
12
ISP-13
ANTIDIABETIC ACTIVITY OF THE VARIOUS EXTRACTS OF GYMNOSPORIA
MONTANA LEAVES.
Sandip Agrawal, Nitin Kochar,Anil Chandewar
P.Wadhwani College of Pharmacy Yavatmal (M.S.)
Abstract
Diabetes mellitus is the serious and most common disorder found in the people worldwide. Many
herbal drugs have successfully been utililised to overcome this problem. Gymnosporia montana
belonging to the family Celeastraceae commonly called as Vikalo is known to possess
antidiabetic properties and used in local health traditions in India but the validation of drug is not
done. Therefore the present study is carried out to investigate antidiabetic properties of different
doses of petroleum ether, ethanolic and aqueous extracts of Gymnosporia montana leaves on
overnight fasted sterptozotocin (50Mg/kg ip) induced diabetic rats.
Ethanolic extracts and aqueous extract of Gymnosporia montana leaves resulted in reduction of
BGL significantly on initial glucose level but petroleum ether extract does not show prominent
effect. Study also confirmed the above findings and the serum lipid profiles, low density
lipoproteins (LDL), very low density lipoproteins (VLDL) and high density lipoproteins (HDL))
and were found to be optimum in ethanolic and aqueous extracts with normal healthy rats or
standard drug glimeperide treated rats.
Thus Gymnosporia montana seems to have a promising value for the development of potent
phytomedicine for diabetes. Leaves extract also exhibit free radical scavenging activity using
DPPH. Antioxidant activity of such extract was attributed to presence of flavonoids and steroids.
13
ISP-14
MODULATION OF SUPER OXIDE DISMUTASE ACTIVITY IN ARTHRITIC RATS

TREATED WITH ETHANOLIC LEAF EXTRACT OF ALBIZIA PROCERA
Sangeetha M1*, Chamundeeswari D1, Saravanababu C2
1
Department of Pharmacognosy, Faculty of Pharmacy, Sri Ramachandra University,
Chennai.
2
Departmentt of Pharmacology, JSS College of Pharmacy & Jagadguru Sri
Shivarathreeswara University, S.S. Nagar, Mysuru.
Abstract
Rheumatoid arthritis (RA) is a chronic, progressive, systemic inflammatory disorder affecting

the synovial joints and typically producing symmetrical arthritis that leads to joint destruction,
which is responsible for the deformity and disability. Despite improvements in the treatment of
RA over the past decade, there still is a need for new therapeutic agents that are efficacious, less
expensive, and free of severe adverse reactions. The present study aimed to investigate the
modulation of SOD activity in arthritic rats treated with ethanolic leaf extract of Albizia procera.
Arthritis was induced by an intradermal injection of 0.1 ml FCA in the foot pad of left hind limb
of rats. ETLE (100 and 200 mg/kg b.wt./p.o) and the reference drug Methotraxate (0.01mg/kg)
were administered to arthritic rats. Paw volume was measured for all the animals before inducing
arthritis and thereafter once in 7 days using plethysmometer for 42 days. Effect of SOD in ETLE
of Albizia procera was evaluated. A significant (P<0.05) decrease in SOD level was observed in
the vehicle treated arthritic rats when compared to the normal rats. ETLE 200 mg/kg showed
significant (P<0.05) elevation when compared to the vehicle treated arthritic rats. These results
provide novel insights into the mechanism of ETLE anti-arthritic activity and unravel potential
therapeutic use of Albizia procera in arthritis.
Key words: Ethanolic Leaf extract (ETLE), SOD-Super oxide dismutase
14
IPS-15
HEPATOPROTECTIVE EFFECT OF Mussaenda frondosa Linn. IN ALBINO RATS

INTOXICATED WITH CARBON TETRACHLORIDE
Shanthi S*, Latha S, Chamundeeswari.D
Department of Pharmacognosy, Faculty of Pharmacy, Sri Ramachandra University, Porur,

Chennai – 600 116.
Abstract
Mussaenda frondosa Linn. (Rubiaceae) is an erect shrub found throughout in India. Whole plant
is used very effectively for the treatment of Jaundice in Indigenous medicine. But no systemic
study to assess this activity has been carried out. The present investigation was carried out to
evaluate the plant drug for its hepatoprotective activity. The hepatoprotective activity of
ethanolic extract of aerial parts of Mussaenda frondosa Linn. was screened at the doses of
100mg/kg , 200mg/kg and 400mg/kg, b.w. in albino rats against carbon tetrachloride induced
hepatotoxicity. The various biochemical parameters viz. Aspartate transaminase (AST), Alanine
transaminase (ALT), Serum Alkaline Phosphatase (SALP) and Serum Bilirubin (SBLN) were
investigated and histopathological studies were also performed. The ethanolic extract of the
plant Mussaenda frondosa Linn has significantly reduced the AST, ALT, SALP and SBLN
levels, which was supported by histopathological studies on liver. The ethanolic extract of the
plant Mussaenda frondosa Linn was proved to be hepatoprotective and justifies the use of this
plant in Indigenous medicine for jaundice.
Key words: Mussaenda, Extract, Heaptoprotective, Jaundice.
15
ISP-16
EFFECT ON ANTICANCER ACTIVITY OF EXTRACT OF NAREGAMI ALATA
WIGHT AND ARN AGAINST DAL AND EAC CELL LINES
P.Akila*, A.Abirami, Mohamed Halith and M.Nagarajan
Department of Pharmaceutics, K.M. College of Pharmacy, Madurai.
Abstract
The objective of the study was to evaluate the phytochemical and anti-cancer activity of
hydroalcoholic extract of Naregamia alata.Naregamia alata (family Meliaceae) is used by various
tribal communities and forest inhabitants for the treatment of a variety of diseases. The leaves
and stems, combined with bitters and aromatics, are given in decoction for the treatment of
biliousness. Naregamia alata has been used in traditional medicines in India and in the
treatment of rheumatism, itch, malarial and chronic fevers, wounds, anaemia, enlarged spleen,
ulcers, vitiated conditions of pitta and vata, halitosis, splenomegaly, scabies, pruritis, dysentery,
dyspepsia and catarrh. Methods The hydro alcoholic extracts of Naregamia alata was analysed
for the presence of flavonoids, alkaloids, glycosides, steroids, phenols, saponins, terpenoid,
cardiac glycosides and tannins according to standard method. Cell lines of Dalton’s Lymphoma
Ascites (DLA), Ehrlich Ascites Carcinoma (EAC) methods have been used in experimental
studies of anticancer activity. In the present study, cell lines induced cancer in mice was used to
evaluate the anticancer activity. Results: The results of the present findings were the potential of
the selected plant as a resource for the discovery of novel anticancer, agents. Conclusion: In this
investigation the various parameters proven that the antitumor activity of hydroalcoholic extracts
of Naregamia alata, in DLA and EAC tumor bearing mice.
Key words: Naregamia alata, phytochemical screening, anti-cancer.
16
ISP-17
PHARMACOGNOSTICAL AND PRELIMINARY PHYTOCHEMICAL SCREENING

OF LAGENARIA SICERARIA
P.Divya*,M. Suganya, K. Murugeswari, V.Balu, M.Packia Lakshmi
K.M.college of Pharmacy, Madurai
Abstract
Lagenaria siceraria( Molina) (Family : Curcurbitaceae) popularly known as Lauki (Hindi) and
Bottle Gourd (English) in India. The plant is reported to have pharmacological activity on
rheumatism, jaundice, also posses emetic, purgative, diuretic, and vermifuge activity.
Pharmacognostical investigation like macroscopy, microscopical characters, determination of
various physicochemical constants like Ash value, extractive value and loss on drying. Aquesous
extract of Lagenaria siceraria has been prepared by maceration process. The aqueous extract is
subjected to preliminary phytochemical screening. The preliminary phytochemical screening
showed that the extract contains Saponins, Tannins, Phenolic compounds carbohydrates,
flavonoids and proteins.
17
ISP-18
IN- VITRO WOUND HEALING ACTIVITY OF DIPTERACANTHUS PATULUS LEAF

EXTRACTS
Esther.M*, Ganesh.H, Dr.Livingston Raja N.R.
KM College Of Pharmacy, Madurai.
Abstract
Medicinal plants are considered as rich resources of ingredients which can be used in drug
development and synthesis of medicines. Wounds are defined as the Rupture of anatomical and
functional honesty of living tissue. Wound healing is a various cellular and biochemical
processes, at last resulting in the conversion and regeneration of damaged tissue. Various plant
products have been used in treatment of wounds over the years. The aim of the Current study
was in-vitro wound healing activity of Dipteracanthus patulus leaf extracts. Dipteracanthus
patulus Leaf was extracted with ethanol and chloroform. Current study was used by in-vitro
chorioallantoic membrane model. Embryonated chicken eggs are used in this model, were
selected then divided into four groups. Small disks were generated with sterilized by autoclaving
and stored for further use. The pre-sterilized filter disks were saturated with different
concentrations of the Dipteracanthus patulus leaf extracts, from 500 μg/ml, and the control
solutions. Diclofenac sodium50 μg/ml and sterile saline were used as positive and negative
controls respectively. Eggshell window was closed and incubated at 370 C for 72 hrs. The growth
of new capillary blood vessels were observed and finally compared. Angiogenesis is vital in
normal processes of the development of blood vessel of embryo. There was concluded by a
significant in the formation new blood vessels as standard compared to positive control group.
Key words: Dipteracanthus patulus, chorioallantoic membrane model.
18
ISP-19
FORMULATION AND EVALUATION OF LORNOXICAM BY USING NATURAL

POLYMERS
J.Priyadharshini , M.ISMATH FARHA*, S. Mohamed Halith, M.Nagarajan
Department of Pharmaceutics, K.M. College of Pharmacy, Madurai, Tamil Nadu.
Abstract
The present work was carried out to prepare and evaluate the topical gel of lornoxicam using
different natural polymers. The gel was prepared by using Sodium alginate, EDTA, Gelatin,
Urea, DMF. The gel was evaluated by using physicochemical parameters (Spreadability, Ph,
Drug content & uniformity, Viscosity, Invitro drug release, Permeability coefficient, Flux for the
gel & Kinetic release of gels). Lornoxicam gel was formulated in 9 formulations. The result
showed that formulation 5% Sodium alginate will produce fruitful results and improve the
patient compliance.
19
ISP-20
PHYTOCHEMICAL STUDIES AND PHARMACOLOGICAL ACTIVITIES OF
ACORUS CALAMUS (வசம்பு)
G. Mukesh*, A.Senthamil Selvi, M. Sundara Pandian, M.Nagarajan

K.M. College of Pharmacy - Madurai
Abstract
In Ayurveda many medicinal plants are described and are widely used by traditional
practitioners for curing and controlling various diseases. Acoruscalamus is one of the
important herbs known for its medicinal properties. It is a perennial, aromatic herb with
creeping rhizomes and the ethnomedicinal and ethnobotanical uses have been ascribed to the
rhizomes of the plant. The active constituents such as β-asarone, α-asarone, acorone,
sesquiterpenes and monoterpenes etc. obtained from the plant rhizomes has been proved to
show various pharmacological activites such as sedative, CNS depressant, behavior
modifying, anticonvulsant, acetylcholinesterase inhibitory, memory enhancing, anti-
inflammatory, antioxidant, antispasmodic, cardiovascular, hypolipidemic,
immunosuppressive, cytoprotective, analgesia, antibacterial, mutagenic, cytotoxic,
hepatoprotective, neuroleptic, insecticidal and larvicidal. This review is done to reveal the
scope and application of Acoruscalamus in the pharmaceutical field.
20
ISP-21
RECENT TRENDS OF PLANT CELL ELICITATION IN PRODUCTION OF

SECONDARY METABOLITES
A. Noorul Asif*, A.G.HariHaran, S. Mohammed Halith and M. Nagarajan
Department of Pharmaceutics, K. M. College of Pharmacy, Madurai
Abstract
The various alternative medicines like sidha, Ayurveda, unani and Chinese medicines, the plants
have been identified for its medicinal values and used as the mode of treatment for different
ailments. The plant still serves as a major source for the discovery of new products. Today
several distinct chemicals derived from plants and its semi synthetic form serve as important
drugs in pharmaceutical industry. The secondary metabolites from plants like alkaloids,
glycosides, flavonoids, volatile oils, tannins, resins etc, are of pharmaceutically significant.
Currently, most of these secondary metabolites are isolated from wild or cultivated plants
because their chemical synthesis is either extremely difficult or economically infeasible. Now a
day’s efforts had been made to produce secondary metabolites by plant cell cultures as an
attractive alternative. The yield of secondary metabolites can be increased by elicitation process
through in vitro technique by the influence of various physical or chemical factors. Over few
decades many strategies like media manipulation, phyto-hormone regulation, precursor feeding,
plant cell immobilization, biotransformation and bioconversion, hairy root cultures and
genetically modified cells etc. have been tried to increase the yield of secondary metabolites.
Elicitation of plant cell culture system may be promising as it showed favorable results in
fermentation of antibiotics and many other fermented products. The focus of the present review
is the application of tissue culture technology for the production of some important plant
pharmaceuticals.
Keywords: Biotic elicitors, Abiotic elicitors, Elicitation, Plant cell cultures, Secondary
metabolites.
21
ISP-22
FORMULATION AND CHARACTERIZATION OF HERBAL LIPSTICK

CONTAINING NATURAL COLORANTS
L.S. Sathyapriya, V.Sathya Praba*, A.G.Hariharan, S. Mohamed Halith and M.Nagarajan
Department of Pharmaceutics, K.M. College of Pharmacy, Madurai, Tamil Nadu.
Abstract
Lipstick is a cosmetic product protects the lips and contains pigments, oils, waxes, and
emollients that apply colour and texture. The herbs used in cosmetic preparation have varieties of
properties like antioxidant, anti-inflammatory, antiseptic and antibacterial etc. These herbal
products claim to have no side effects, commonly seen with products containing synthetic
agents. The literature in Ayurveda, especially Charak Samhita, stated numerous
medicinal plants in Varnya kashaya. The herbs like Chandan, Haldi, Khas, Nagkheshara,
Manjistha, and Yastimadhu are used to obtain glowing complexion and Arusa, Amala,
Bavchi, Guduchi, Chakmard are mentioned as Kustaharan Herbal cosmetics have growing
demand in the world market and are an invaluable gift of nature. There are a wide range of
herbal cosmetics products to satisfy your beauty regime, adding herbal in cosmetic is very safe
for skin. Betanin, or Beetroot Red, is a red glycosidic dye obtained from beets. As a food
additive, its E number is E162 but the Betanin degrades when subjected to light, heat, and
oxygen. The objective of the present work is to extract the colored pigments from Beta vulgaris
taproot, optimize the formula for the preparation of lipstick and evaluate the prepared
formulations. Out of the six formulations prepared, consistency was uniform in two formulations
and all the formulation does not have any skin irritancy. The formulation F4 had good stability.
22
ISP-23
EFFECT OF FIBRINOLYTIC ACTIVITY ON HIBISCUS ROSA SINENSIS
A.Aruna, S.MohamedHalith, M.Nagarajan, S.Soniya*
Department of pharmaceutics, K.M College of pharmacy, Madurai.
Abstract
The main function of the fibrinolytic system is to dissolve fibrin clots in circulation. This system
is composed of inactive precursor plasminogen which can be converted into plasmin by the
proteolytic enzymes like tissue-type plasminogen activator (tPA). Fibrinolytic properties can be
found in a variety of medicine plants. One of these medicine plants isHibiscus rosasinensis,
which was used for its fibrinolytic effects in patients. This study considers finding the
fibrinolytic effect of isolation of various fractions of aqueous extract ofHibiscus rosasinensis.The
RBC cell count wasexamined under microscope by haemocytometer. The result showed that the
aqueous extract ofHibiscus rosasinensisdisplayed significant fibrinolytic activity.
Keywords: Fibrinolytic activity, Hibiscus rosasinensis, aqueous extract.
23
ISP-24
PREPARATION AND STANDARDISATION OF HERBAL JUICE (FICUS CARICA

LINN)
Thulasimani P*, Mohamed Shiek Arabath S.A, Seshu Kumar Koduri L, Saujania D, Moorthy P
K.M.College of Pharmacy, Madurai - Tamilnadu
Abstract
Fig fruit is from Ficus carica tree belongs to the family Moraceae. The Fruit when fresh possess
Protein 1.3 % , Minerals 0.6 %,Carbohydrates 17.1 % , Calcium 0.06% , Phosphorus 0.03 % ,
Iron 1.2 Mg , Carotene 2701.U. , Nicotinic acid 0.6 Mg , Riboflavin 50 Mg , Ascorbic acid 2 Mg
Per 100 grams . The sugar content in fresh fruit 13-20 % and dry fruit has 12 to 62 %. Mainly
Fig preparations are used as a Laxative. In china Figs are used for Dysentery and Enteritis.The
aim of the study is to Prepare and standardise a herbal juice (Ficus carica Linn). Dry fruits of
Ficus carica were collected from departmental store in Hyderabad and prepare juice from that.
The juice is subjected to evaluate Viscosity, Weight per ml, PH determination and Phyto
chemical investigation. The result of phytochemical investigation shows that the fruit contains
carbohydrates, proteins and flavonoids. The formulation is pleasant in appearance, taste and
odour. Hence we believe this formulation would attract the consumer and increase the patient
compliance.
Key Words: Figs, Nutraceuticals, Evaluation, Juice
24
ISP-25
EFFETCT OF VITEXIN ON HYPOXIC STRESS IN SOLID TUMORS: AN INSIGHT
Sandhya Chipurupalli1*, Elango Kannan1
Department of Pharmacology, JSS College of Pharmacy, Ooty, Tamil nadu, India – 643001.
Abstract
Cancer is a growing public health concern in both developed and developing countries. Several
plant-derived anti-cancer agents including taxol, vinblastine, vincristine, the campothecin
derivatives, topotecan, irinotecan and etoposide are in clinical use all over the world. Other
promising anti-cancer agents include flavopiridol, roscovitine, combretastatin A-4, betulinic acid
and silvestrol. From this one can well imagine the predominance of polyphenols, flavonoids and
their synthetic analogs in the treatment of various cancers. Flavonoids comprise many
polyphenolic secondary metabolites with broad-spectrum pharmacological activities including
their potential role as anti-cancer agents. Vitexin, an apigenin flavone glucoside, is known to
exhibit broad anti-oxidative, anti-inflammatory, analgesic, and antitumor activity in many cancer
models. Solid tumors are often challenged by hypoxia, lack of glucose and nutrients, due to poor
vascularization upon rapid expansion of the tumor mass. This results in development of severe
stress in the endoplasmic reticulum (ER) because of the accumulation of misfolded or unfolded
proteins which are formed in the ER due to lack of oxygen required for the formation of
disulphide bond during protein folding or isomerization. The condition is termed “ER stress”,
which ultimately activates Unfolded Protein Response (UPR). Thus, hypoxic tumor
microenvironment has a direct impact on protein modification in the ER. Hypoxia-inducible
factor 1 (HIF-1) was identified as the most important mediator in hypoxia. Hypoxia is also
known to cause tumor resistance to chemotherapy as well as radiation therapy. Thus the current
study was focused to investigate the effects and underlying mechanisms of vitexin in the
treatment of tumor hypoxia.
Keywords: Vitexin, Endoplasmic reticulum stress, hypoxia, hypoxia-inducible factor 1,

normoxia, solid tumors, unfolded protein response.
25
ISP-26
PRELIMINARY PHYTOCHEMICAL SCREENING AND HPTLC FINGERPRINT

ANALYSIS OF ISOLATED FREE AMINO ACIDS FROM LEAVES OF Alternanthera
sessilis
Mrs.S.Swarnakumari*, Mr.M.Karthickraja, Dr.V.E.Ida Christi, Dr.S.Mohan
Karpagam College Of Pharmacy, Coimbatore -32
Abstract
The analysis and quality control of herbal medicines are moving a step ahead towards an
integrative and comprehensive direction, in order to tackle the complex nature of herbal
medicines. High-performance thin layer chromatography (HPTLC) is one of the sophisticated
instrumental techniques for qualitative and quantitative analysis of the herbs and herbal drugs.
Free amino acids have been isolated from the leaves of Alternanthera sessilis and qualitatively
analyzed using High performance thin layer chromatography. Preliminary phytochemical
screening was done. Free amino acids were isolated from leaves and HPTLC studies were
carried out for isolated amino acids. CAMAG make HPTLC system equipped with Linomet 5
applicator, TLC scanner 3 and WIN CATS- 1.4.3 software were used. Preliminary
phytochemical screening of the extract indicate the presence of alkaloids, carbohydrates, sterols,
protein and free amino acid, tannin and flavonoids. Since rich in protein, various free amino
acids have been isolated in aggregates and chromatographic techniques have been used to
identify the isolated amino acids. HPTLC fingerprinting analysis of isolated amino acid reveals 7
peaks with Rf values 0.34, 0.42, 0.54, 0.60, 0.72, 0.89 and 1.01 and qualitatively compared with
the standard amino acid. The present study indicate that the plant is rich in amino acid which can
be isolated and in future can be analyzed quantitatively.
Keywords: leaves of Alternanthera sessilis, phytochemical, Amino acids, HPTLC.
26
ISP-27
PREDICTION OF MILD COGNITIVE IMPAIRMENT USING HIGH-DENSITY

LIPOPROTEIN AND MINI-MENTAL STATE EXAMINATION.
Menge D.M.1*, Nair N.1, Vijayakumar A.P.R.1

1
Deparment of Pharmacy Practice, JSS College of Pharmacy, Ooty-643001, JSS University,
Mysore, INDIA
Abstract
Mild cognitive impairment (MCI) is an intermediate stage between normal cognitive decline due
to ageing and a more serious decline of dementia. It has become useful both in clinical and
research entities. MCI cases over time could progress to Alzheimer’s disease and other dementia
types, become stable or even recover. MCI could be amnestic, single non-memory domain or
involve multiple domains.
A group of 356 patients were recruited into the study based on inclusion and exclusion criteria.
Patients with Alzheimer’s disease, Parkinson’s disease, and Dementia were excluded from the
study. HDL levels of the subjects were then measured. Mini-Mental State Examination (MMSE)
was administered. MMSE was used determine the general cognition and memory. The total sum
of the scores obtained from the Mini-Mental State Examination was correlated to the
corresponding levels of HDL to determine the relationship. By this method, it will be possible to
predict early onset of cognitive impairment, which could prove to be useful to physicians in
planning better on how to manage MCI in the individuals. It will also help the patients along
with their carers to better understand MCI and how to manage it accordingly.
Keywords: Mild cognitive impairment, Alzheimer’s disease, Dementia, High-density

lipoprotein.
27
ISP-28
EVALUATION OF PHYSICOCHEMICAL AND BINDING CHARACTERISTICS OF
NOVEL NATURAL MUCILAGE IN THE FORMULATION OF TABLET
Prince.R ,Ramya Devi.A*,Pradeesh Mohan.S, Gayathri.R, Mohan.S
Abstract
Tablets due to their portability are the most widely prescribed dosage form. One major class
of excipients used in the tablet formulation is pharmaceutical binders. A tablet binder is used in
oral dosage form to hold the active and inactive pharmaceutical ingredient and hence improves
bonding properties on granulation. Binders ensure that tablets and granules can be formed with
required mechanical strength and give volume to low active dose tablet. The present study deals
with isolation of mucilage from the leaves of Hibiscus sabdariffa, its safety and its application
as binding agent in Paracetamol tablet as model formulation. Extraction and isolation of
mucilage was carried out and organoleptic properties were evaluated. It shows positive test for
carbohydrate ,mucilage in molisch’s and ruthenium test respectively and the iodine test gave
negative result ,thus polysaccharides is confirmed. The flow properties of granules prepared with
mucilage has good compressibility as that of the granules formulated with starch and PVP.Post
compression parameters formulated with mucilage had better hardness and friability. The
formulation exhibited a better and most consistent release as compare to standard formulations
using starch and PVP as binders. Based on the above consideration, the study has revealed a
good potential of Hibiscus sabdariffa mucilage as binder in tablet formulations.
28
ISP-29
PHYSICOCHEMICAL CHARACTERISATION AND BINDING PROPERTY OF

POLYSACCHARIDE OF SOLANUM BETACEUM CAV IN TABLET FORMULATION
Abinaya.M* ,Aravind.M, Prasanth.R.K, Benedict Jose.C, Mohan.S
Abstract
Binders are pharmaceutical excipients that are commonly employed in tablet formulation to exert
cohesion on the powder mix there by improves the binding property of the granules. Binders
improve the formation of granules of desired hardness and size. An ideal binder should have
good binding properties, as determined by compressibility under pressure, high plasticity, low
elasticity and small particle size. The study deals with the extraction of polysaccharide from the
fruit of the solanum betaceum cav and to study the characteristics of the polysaccharide as a
binding agent in the formulation of tablets. The mucilage was precipitated and polysaccharide
was isolated. Organoleptic properties were evaluated and its shows positive test for carbohydrate
,mucilage in molisch’s and ruthenium test respectively and the iodine test gave negative result
,thus polysaccharides is confirmed. On the basis of this study polysaccharide isolated from the
fruit of solanum betaceum cav shows excellent binding property with no interaction in it
comparisons with existing polymers.
29
ISP-30
CORRELATION BETWEEN COGNITIVE IMPAIRMENT AND THE QUALITY OF
LIFE IN PATIENTS WITH TYPE 2 DIABETES MELLITUS
V.Sreedevi1*, P.R.Anand Vijaya Kumar2.
Department of Pharmacy Practice, JSS College of Pharmacy, Ooty.
Abstract
The current study was designed to estimate the prevalence and factors influencing cognitive
impairment in type 2 diabetes mellitus (T2DM) patients and to find out the correlation between
cognitive functions and quality of life. A cross sectional study was performed on 542 T2DM
patients, aged 25-85 years in Coimbatore, a second largest city in TamilNadu, located in
southern India . Cognitive function score and QOL were measured using Mini-Mental State
Examination (MMSE) and SF12 Version 2 respectively. Statistical analysis was performed using
in IBM SPSS (version 21). The prevalence of cognitive impairment was found to be 20.48%
among 542 T2DM patients. Compared with the studies from northern India the prevalence of
cognitive impairment in type 2 diabetes is found to be less in southern India. This difference may
be due to variations in their lifestyles and dietary intake. Socio-demographic factors including
age (p=0.001), gender (p=0.001), education (p=0.001), physical activity (p=0.001), and duration
from onset of type 2 diabetes mellitus (p=0.029) was significantly associated with cognitive
impairment. A significant positive correlation was found between cognitive functions and quality
of life (p<0.001) (r = 0.446). The present study confirms a significant positive correlation
between cognitive functions and quality of life and the moderate prevalence of cognitive
impairment among type 2 diabetes mellitus patients in southern India. As a concomitant factor
with type 2 diabetes, cognitive impairment might exacerbate the clinical picture.
30
ISP-31
MEDICINAL PURPOSES, ORIGIN AND EFFECTIVENESS OF INDIAN

SYSTEM OF MEDICINE ALOE VERA
Yukesh P,M.Swarnakumari,V.E.Ida Christi, S.Mohan
Karpagam College of Pharmacy, Coimbatore-641032
Abstract
Indian system of medicine is the well-known global traditional systems of medicine are
of great relevance to the health care of the people. It is calculated that majority of world
population are still relying on various traditional systems of medicine is separates into Ayurveda,
Siddha, Unani, Homeopathy and Naturopathy respectively. Interest in them and utilization of the
herbal products produced based on them is increasing in developed countries. In this abstract, an
attempt has been made to provide short general information about different systems and large
explanation about the Aloe vera. Aloe vera is a plant species of genus ‘Aloe’ under family
Xanthorrhoeaceae. It grows wild in tropical climates around the world and is cultivated for
agricultural and medicinal uses There is little scientific evidence of the effectiveness or safety of
Aloe vera extracts for either cosmetic or medicinal purposes. Under research , it has having
more beneficial effects of human health care. It cause positive effectiveness among the female
menstrual problems. Aloe vera leaves contain phytochemicals under study for possible
bioactivity, such as acetylated mannans, polymannans, anthraquinone C-glycosides, anthrones,
other anthraquinones, such as emodin and various lectins. Aloe vera found in different colours
and types in various types of land . It is found in many consumer products including beverages,
skin lotion, cosmetics, or ointments for minor burns and sunburns.
31
ISP-32
PREVALENCE OF DEPRESSION AND ASSOCIATED FACTORS IN PATIENTS

WITH ACUTE CORONARY SYNDROME
Treesa P. Varghese1 and P. R Anand Vijaya Kumar2
1
Research Scholar, Department of Pharmacy Practice, JSS College of Pharmacy, Ooty
2
Professor, Department of Pharmacy Practice, JSS College of Pharmacy, Ooty
Abstract
Depression is one of the major psychological symptoms among patients with Acute Coronary
Syndrome (ACS) but may remain under diagnosed and undertreated. This study aimed to assess
the frequency of depressive symptoms among patients with ACS and different factors associated
with it.
A prospective observational study was carried out for a period of one year. 400 patients with
ACS were selected for the study. Patient’s socio demographic factors, duration since diagnosis,
co morbidities associated with Acute Coronary Syndrome and intervention at the time of
diagnosis were collected from the patient medical records and interview methods. Hamilton
Depression Rating scale (HAM –D) questionnaire was administered to assess the depressive
symptom among patients with ACS.
Of the 400 ACS patients 65.5% were male and 34.5% were females. Mean age of the male and
female were 63.67±10.62 and 66.71±8.98 respectively. A large prevalence of ACS was found in
the age group 61-70years. Our result indicates that, the factors like type of living and
intervention at the time of diagnosis were significantly associated with gender. ACS more
commonly associated with male patients whereas the depressive symptoms more predominant in
women. The study revealed that, Depressive symptoms significantly correlated with age, gender,
duration since diagnosis and the number of co morbidities associated with ACS
It can be concluded that, large proportion of ACS patients were under depressive symptoms.
Hence, it is advisable for the proper screening of depression in this population for earlier
intervention especially patients who already presented with multiple risk factors.
32
ISP-33
CURRENT CONCPENTS IN NUTRACETICALS AND USES OF FUNCTIONAL

FOODS
Parameshvar.R*,Dr.S.Mohan
Karpagam College of Pharmacy, Coimbatore - 641 032, Tamil Nadu, India.
Abstract
Nutraceuticals and functional foods are important for health promotion and disease risk
reduction. The term "Nutraceutical" was coined from NUTRITIVE + PHARMACEUTICAL in
1989. Nutraceutical has advantage over the medicine because they avoid side effect, have
naturally dietary supplement, etc. The most rapidly growing segments of the industry were
dietary supplements (19.5 percent per year) and natural/herbal products (11.6 percent per year).
Global nutraceutical market is estimated as USD 117 billion. FDA regulated dietary supplements
as foods to ensure that they were safe. In 2006, the Indian government passed Food Safety and
Standard Act to regulate the nutraceutical industry. Herbal nutraceutical is used as a powerful
instrument in maintaining health and to act against nutritionally induced acute and chronic
diseases, thereby promoting optimal health, longevity, and quality of life. They possess
numerous therapeutic benefits like anti-obesity, immune enhancement, natural antioxidant,
cardiovascular effects, anti-diabetic, anti-inflammatory effects, etc. Major constituents of
nutraceuticals are nutrients, herbals and dietary supplements which help to maintain health,
function against various diseases and ensure better quality of life. Functional foods contains
many potential benefits like Carrot(beta-carotene) for neutralize free radicals, Tomato
(Lycopene) for reduce the risk of prostate cancer, Salmon(DHA) for reduce risk of
cardiovascular disease. Thus, this review gives a idea about the current status in the field of
nutraceuticals and uses of functional foods.
33
ISP-34
CLINICAL PHARMACOGNOSY- CATHARANTHUS ROSEUS
Hemapriya Nachiyar.K, S.Mohan
Karpagam College of Pharmacy, Coimbatore-32
Abstract
Clinical pharmacognosy – integration between pharmacognosy and clinical pharmacy.Clinical

pharmacy can provide information such as the efficacy, adverse action, or interactions of crude
drugs or natural products as well as chemical drugs.The term pharmacognosy was first
introduced between 1811-1815,and formerly referred to “materia medica" Plants have been
appropriate source of medicines for past era.The essential features of pharmacognosy have
important application on a wide range of drugs . The crude form of a drug contains many active
constituents and separation of them without special technique or procedures is not so easy .In the
developing world , herbs used for medicinal purposes are “crude drugs". The isolation and
characterization of pharmacologically active compounds from medicinal plants persist today.
Clinical pharmacognosy is a link between botanicals and clinical research knowledge providing
clinical and pharmaceutical researchers, physicians and other healthcare professionals with the
key information they need to assist the progress of herbal and traditional medicines.
Catharanthus roseus, commonly known as the Madagascar periwinkle belongs to the family
Apocynaceae .Grown as ornamental and medicinal plant , a source of the drugs vincristine and
vinblastine . Vinblastine and vincristine are biosynthesized from the coupling of the alkaloids
catharanthus and vindoline . Rosinidin is an anthocyanidin pigment found in the catharanthus
roseus. Vinblastine and vincristine , chemotherapy medications used to treat several types of
cancers. Semi-synthetic chemotherapeutic agent VINORELBINE used in the treatment of Non-
small-cell lung cancer. Herbal medicines in ayurveda for the treatment of cancer are GUGGUL
(COMMIPHORA WIGHTII),CARDAMOM(ELETTARIA CARDAMOMIUM) etc.,
34
ISP-35
ASSESSMENT OF QUALITY BY DESIGN (QBD) TO DEVELOP AND VALIDATE RP

HPLC-PDA METHOD FOR THE SIMULTANEOUS ESTIMATION OF
PHYTOSTEROLS IN MANASAMITRA VATAKAM AN AYURVEDIC HERBO
MINERAL FORMULATION
Srikalyani V1, R. Mohan Kumar1, R. Pandiyan1, R.C. Satish Kumar2, K. Ilango1, 3*
1
Division of Analytical Chemistry. 2
2
Clinical Trial and Research Unit (Metabolic Ward), Interdisciplinary Institute of Indian System
of Medicine (IIISM), SRM Institute of Science and Technology, Kattankulathur- 603 203,
Kancheepuram (Dt), Tamil Nadu.
3
Department of Pharmaceutical Chemistry, SRM College of Pharmacy, SRM Institute of
Science and Technology, Kattankulathur- 603 203, Kancheepuram (Dt), Tamil Nadu.
Abstract
A new method was developed to approach multiresponse optimization coupled with response
surface methodology for simultaneously optimizing a large number of experimental factors. The
present study involves developing a simple, efficient, precise and robust RP HPLC-PDA method
for the quantification and validation of Stigmasterol (STG) and β-Sitosterol (STS) in
Manasamitra Vatakam. LC Chromatogram was optimized by using Phenomenex RP C 18 column
(250mm x 4.6mm; 5µm); mobile phase was pumped at isocratic mode with a flow rate of 1.0
mL/min using acetonitrile and methanol (5: 95 % v/v) at the detection max of 208 nm with the
retention time of 16.3 and 18.1 min for STG and STS respectively. The amount of STG and STS
was quantified and found to be 51.0 and 56.3 µg/mg respectively. The validation parameters for
the developed method were done as per ICH Q2B guidelines. The method was found to be linear
in the range of 80-130 µg/mL with r2 0.9945 and 0.9960 for STG and STS respectively. LOD &
LOQ were 0.0507, 0.1537 µg/mL and 0.0594, 0.1800 µg/mL of STG and STS respectively. The
system precision and the method precision were found to be 0.94%, 0.40 % and 1.51%, 1.1% (≤
2%) for STG and STS. Recovery studies in the range of 80, 100 and 120% were performed and
found in the range of 95-105% indicates the accuracy of the method. For the evaluation of the
analytical robustness, fractional factorial design method was employed to validate the efficiency
of the method. The developed method is the first report for the simultaneous estimation of STG
and STS in Manasamitra Vatakam.
35
ISP-36
QUANTIFICATION AND VALIDATION OF QUERCETIN BY RP-HPLC IN THE

LEAF EXTRACT OF HUBERANTHA SENJIANA - AN ENDEMIC TREE OF GINGEE
HILLS, TAMIL NADU, INDIA.
R. Pandiyan1, R. MohanKumar1, V. SriKalyani1, K. Ilango1, 2*

1*
Division of Phytochemistry and Pharmacognosy, Interdisciplinary Institute of Indian System of
Medicine (IIISM), SRM Institute of Science and Technology, Kattankulathur- 603 203,
Kancheepuram (Dist), Tamil Nadu, India.
2
Department of Pharmaceutical Chemistry, SRM College of Pharmacy, SRM Institute of Science
and Technology, Kattankulathur- 603 203, Kancheepuram (Dist), Tamil Nadu, India.
Abstract
Quercetin, a polyphenolic flavanol, has been identified and quantified from the foliage of
tropical evergreen forest tree, Huberantha senjiana (Annonaceae) - an endemic tree of Pakamalai
Reserve forest Ginjee hills, Villupuram District, Tamil Nadu, India. This is the first
phytochemical report on the presence of flavonoid in this new species. Preliminary
phytochemical tests revealed the presence of flavonoids in the ethyl acetate extract of the leaves.
A reversed-phase high performance liquid chromatographic (RP-HPLC) method was developed
and validated for the quantification of quercetin. The chromatographic separation was
accomplished on a Phenomenex, RP C18 column with an isocratic mobile phase consisting of
[(H2O (0.1% Formic acid, A); (MeOH: ACN, (40:15v/v), B), 40:60(v/v)] and a flow rate of 0.8
mL/min. The detection was recorded by a PDA detector at 254 nm. The retention time of
quercetin was found to be 7.58 min.
The proposed method was validated according to the ICH Q2B guidelines. Linearity was
achieved, over the concentration range of 5.0-17.5μg/ml. The correlation coefficient of the
calibration showed good linearity (R2 = 0.996). High sensitivity was demonstrated with a
detection limit of 2.28µg/mL (LOD) and 6.92 µg/mL (LOQ). The robustness was evaluated by
deliberate changes in the composition of mobile phase, flow rate, and wavelength and the limits
were found to be NMT 4%. Huberantha senjiana was found to contain 0.00513 % w/w of the
foliar concentration of quercetin. This is the first published report on the identification of
quercetin in H.senjiana species. Further investigations exploring the phytochemical profile will
result in new drug leads.
36
ISP-37
EFFECTS OF STANDARDIZED POLYHERBAL FORMULATION ON DEPRESSION

AND SLEEP QUALITY AMONG GERIATRIC PATIENTS: A POLYSOMNOGRAPHIC
INTERPRETATION
I.Jyothi1*, R. Mohan Kumar2, N. Kasthuri Bai2 , R.C. Satish Kumar1, K. Ilango2
1*
Clinical Trial and Research Unit, Interdisciplinary Institute of Indian System of Medicine
(IIISM), SRM Institute of Science and Technology, Kattankulathur-603 203, Kancheepurnam
(DT), Tamil Nadu, India
2
Division of Phytochemistry and Pharmacognosy,Interdisciplinary Institute of Indian System of
Kancheepuram (DT), Tamil Nadu, India
Abstract
Sleep has received increasing attention within the context of geriatric research based on a
growing body of evidence that links poor sleep with many adverse health outcomes, especially
decline in cognition, in older adults. Age-related decrease in the amplitude of melatonin
secretion contributes to the increased sleep disruption in older adults. Therefore, identifying a
cognitive enhancing drug that can be used without adverse effects in geriatric population has
captured the interest of researchers in neuroscience. The present study was designed to evaluate
an U.S. Patented (Patent No. US9675657 B2) polyherbal formulation [Bacopa monnieri (whole
plant), Hippophae rhamnoides (leaves and fruits) and Dioscorea bulbifera (bulbils)] on memory
performance, depression and sleep quality among mild to moderately depressed geriatric
population. Role on melatonin regulation was also studied. Polysomnographic sleep parameters
in the placebo treatment were compared with the treatment of poly herbal formulation. Sleep
efficiency was significantly increased in the treatment group with herbal formulation
(78.59±5.50%) When compared to placebo group (65.41±6.12%) Similarly, wake time also
decreased significantly compared to placebo treatment, but Stage 1 (N1), Stage 3 (N3) and REM
sleep were significantly increased. Phyto constituents like bacosides and quercetin were
quantified in polyherbal formulations by RPHPLC-PDA method and were found to be 4.565%
w/w and 3.759% w/w of extract in Polyherbal formulation respectively. In baseline there was a
lower level of melatonin at night and higher morning melatonin levels at morning in both
placebo and treatment groups. There was no significant alteration of biochemical,
hematological, hepatic or renal parameters. Most patients tolerated test drug well without any
major side effects.
37
ISP-38
ISOLATION, CHARACTERIZATION AND MOLECULAR DOCKING STUDIES OF PYRIMIDINE

ANALOGUE FROM DESMODIM TRIFLORUM LINN.
Vedpal*, S.P.Dhanabal, P.Dhamodaran
Department of Pharmacognosy , JSS College of Pharmacy, Udhagamandalam- 643001
Jagadguru Sri Shivarathreeshwara University, Myssuru, India,
Abstract
The present study focused on Extraction, Isolation and Characterization of Pyrimidine Analogue from
Desmodium species belongs to Fabaceae Family and In Silico molecular studies to confirm the receptor
binding for (TNF-α, IL-13 and IL-4) inhibition activity.
Based on literature survey like Google, Pubmed and scientific traditional books related to ethano-
Pharmcological evidence the plant were selected for this study and collected from Aminabad- Lucknow and
identified from National Botanical Research Institute Lucknow. UP. India. After that plants were shade dried
and powdered and proceed for Hydro-alcoholic maceration (50:50) and percentage yield of extract was
calculated based on dried basis. The 20 gm of extract further proceed for fractionation and isolation using
Column Chromatography and the isolated compounds were placed for phytochemical screening and identified
by GC-MS and Infrared spectroscopy.
The percentage yield of extract was found to be 4.3% and the same extract and fractions were subjected to
preliminary phytochemical screening revealed that the presence of Alkaloids and Flavonoids. The total number
of five fractions was isolated (F1 to F5) (F5 Chloroform fraction). Thin layer chromatography was performed
using standard methods (Harborne, 1998). Small quantities of samples (2 mg/ml) were taken and dissolved in
their respective solvents and Rf value was calculated (0.51). The same compounds were subjected for GC-MS
and Infra Red based on interpretation the compounds were identified as pyrimidine analogue (Pyrimidin2one,
4[Nmethylureido] 1[4methylaminocarbonyloxymethyl, Molecular formula C13H19N5O5 Molecular weight
325.325). In-silico molecular docking was performed showed good G-Score against different Asthma targets
(TNF-α, IL-13 and IL-4).
Keywords: Desmodium Triflorum Linn, Fabaceae, GC-MS, Infra Red and G-Score
38
ISP-39
SIMULTANEOUS ESTIMATION AND VALIDATION OF PAMABROM AND

PARACETAMOL BY HPLC METHOD IN EXTENDED RELEASE TABLETS
Madhan S*, Nowfal Habeeb A, Manikandan K, Lakshmi K S.,
Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM University,
Kattankulathur – 603 203, Tamilnadu.
Abstract
A sensitive reversed phase high-performance liquid chromatographic (HPLC) determination of

Pamabrom and Paracetamolin Extended Release tablets has been developed. Inthe HPLC
methodPamabrom and Paracetamolwas separated using PhenomenexC18 column and
Acetonitrile: 0.2% w/v of Trichloro acetic acid (4:96 ; v/v) the mobile phase system; the speed of
the mobile phase flowing was 1.0ml/min and thedetection was actualized at 289 nm. The
chromatographic method was linear over the concentration range of 50 –300 µg/ml for
Pamabrom and Paracetamol. The correlation coefficient of Pamabrom and Paracetamolwas
found to be 0.9990 and 0.9998 respectively. The limit of detection and limit of quantitation were
2.94µg/ml and 18.91µg/ml for Pamabrom and 9.30µg/ml and 28.19µg/ml for
Paracetamolrespectively. The mean percentage recovery was found to be 101.06 % and 100.39%
for Pamabrom and Paracetamolne respectively. The method was found to be accurate and precise
and hence can be applied for routine analysis of pharmaceutical dosage forms.
Key words: High Performance Liquid Chromatography (HPLC); ICH Guidelines;Pamabrom:

Paracetamol; Validation; stability studies.
39
ISP-40
FORMULATION AND EVALUATION OF TOPICAL GEL LOADED WITH

ANTIBACTERIAL AGENT
Dr. M.S.Umashankar, A. Nowfal Habeeb*, S. Madhan, Vishnu R
Department of Pharmaceutics,
SRM College of Pharmacy Kattankulathur, Kancheepuram – 603 203
Abstract
Cefixime is an antibiotic drug used for the treatment of local and systemic infections. The topical
gel contains polymers such as HPMC, Carbopol-940 and solvents like propylene glycol, ethanol,
TEA, oleic acid, Tween-80 and glycerine. The oral use of Cefixime is not recommended as it has
many side effects. The present study was designed to formulate and evaluate different formulae
of topical gel containing Cefixime for treatment of bacterial infections. The antibiotic gel was
formulated by using different polymers with different concentration as Carbopol 940,
Hydroxypropyl methylcellulose E4M, Methyl cellulose. Two different formulation were
prepared and characterized physically in terms of color, syneresis, spreadability, pH, drug
content and rheological properties. In-vitro drug release in phosphate buffer pH 7.4 and
permeation study through cellulose membrane using self-designed diffusion apparatus were
performed. The results of in vitro drug release studies showed that the release of the drug from
the selected best two formulations F2 and F3 were 32.2% and 29.3% respectively after 2 hrs. The
release data analysis using kinetic model revealed zero order drug release. The drug permeation
study showed a linearity for the formulation F2. Also F2 showed the highest antibacterial
activity. The rheological behavior of the prepared formulae showed shear-thinning flow
indicating structural breakdown of the existing intermolecular interactions between polymeric
chains. Moreover, the stability study revealed no significant difference between before and after
storage for selected formula.
Keywords: Cefixime, antibacterial activity, gel, topical formulation.
40
ISP-41
IN -VITRO BIOLOGICAL SCREENING OF THE ANTICHOLINESTERASE AND

BUTYRYLCHOLINESTERASE INHIBITORY ACTIVITIES OF SOME
TRADITIONALLY USED MEDICINAL PLANTS OF SOUTH INDIA FOR THE
MANAGEMENT OF ALZHEIMER’S LIKE CONDITION
Swati som1, Dr. Justin Antony2, Dr. S. Ponnusankar1

1
Department of Pharmacy Practice, JSS College of Pharmacy, Ootacamund, Tamilnadu, India
Department of pharmacy 2Pharmacology, JSS College of Pharmacy, Ootacamund, Tamilnadu,
India
Abstract
Clinical treatment and management of Alzheimer’s disease (AD) is the challenging task due to
lack of effective therapy availability and offer symptomatic benefits, providing temporary
cognitive improvement and deferred decline with little or no evidence of slowing disease
progression. Currently available drugs are directly or indirectly derived from herbal resources
such as Galantamine, Rivastigmine etc. indicating natural products can be invaluable for drug
development. In siddha system of medicine, numerous plants have been used to treat various
neurological disorders such as AD.
The aim of the proposed research is to assess and validate the cognitive enhancement activity of
selected plants by screening for both acetylcholinesterase (AchE) and butyrylcholinesterase
(BuchE) inhibition by using In-Vitro Ellman's method.
Present studies confirmed that out of all the tested extracts Hemidesmus indicus R.Br (HI)
showed considerable IC50 values for AchE (28.40 ± 0.92 μg/mL) and BuchE (43.47 ± 0.64
μg/mL) inhibition which indicates that HI extract has considerable specificity toward AchE and
BuchE compared with all the tested extracts followed by Vernonia anthelmintica (VA) Willd
extract with IC50 values for AchE (36.44 ± 0.89 μg/mL) and BuchE (74.62 ± 0.84 μg/mL)
inhibition. Furthermore, novel lead compounds from HI and VA extracts will be isolated and
chemically characterize and possible mechanism for cognitive enhancement activity of
fractionated indigenous plants extracts as well as isolated novel compound will be elucidated by
performing neurochemical and neurotransmitters estimation followed by gene expressions,
immunohistochemistry studies by using molecular techniques like ELISA, RT-PCR, western
blot analysis in specific regions of brain.
Keywords: Acetyl cholinesterase, Alzheimer's disease, butyrylcholinesterase,

Neurotransmission, Neuroprotection
41
ISP-42
A FIRST REPORT ON PHYTOCHEMICAL COMPARISON AND SIMULTANEOUS

ESTIMATION OF LUPEOL, STIGMASTEROL AND Β- SITOSTEROL IN ADHATODA
BEDDOMEI CLARKE AND ADHATODA VASICA NEES LEAF EXTRACTS BY RP-
HPLC-PDA METHOD
S. Nandhini1, R. Mohan Kumar1, N. Kasthuri bai1, K. Ilango1, 2*
1Division of Pharmacognosy and Phytochemistry, Interdisciplinary Institute of Indian System of

Kancheepuram (DT), Tamil Nadu, India
Abstract
Adhatoda vasica Nees is a well-known medicinal plant, used in Ayurveda and an official drug in
Indian Pharmacopoeia. Adhatoda beddomei Clarke known as Chittatalodakam in Tamil and
rarely found in Western Ghats of Kerala and Tamil Nadu. Traditionally, both the species of
Adhatoda have been used for various diseases especially in asthma, skin diseases, bronchitis,
tuberculosis and leprosy etc., A. beddomei is a new plant species, and not yet explored
scientifically. Hence the present study has been focused to develop a RP-HPLC method for the
simultaneous estimation of lupeol, stigmasterol and β-sitosterol in A. beddomei and A. vasica.
Chromatographic separation was achieved by HPLC using Phenomenex RP, C18
(250mmX4.6mm, 5μ) column maintained at an ambient temperature. The optimum mobile phase
consisted of methanol and water (0.1% formic acid) in the ratio of 98:2 with a flow rate of 1.2
ml/min and detected at 208nm. The linearity was investigated in the range of 12.5-200μg/ml with
six different concentrations and the calibration curves for lupeol, stigmasterol and β-sitosterol
were linear, with correlation coefficients (r2=0.9997, 0.9994 and 0.9978, respectively). A.
beddomei and A. vasica contain varying amounts of alkaloid, phenolic and flavonoid
compounds, as well as varying concentrations of triterpenoid, lupeol and sterols stigmasterol and
β-sitosterol. The highest percentage of lupeol, β-sitosterol (0.9817%w/w, 3.1258%w/w) and
stigmasterol (1.2695%w/w) were found in the hexane extract of A. vasica and A. beddomei
respectively. The developed HPLC method is the first report on the simultaneous estimation of
lupeol, stigmasterol and β-sitosterol and concluded that, hexane is the suitable solvent for
isolating lupeol, stigmasterol and β-sitosterol in the leaf extract of A. beddomei and A. vasica.
42
ISP-43
THE DOCUMENTATION OF IRULA MEDICINAL PRACTICE AGAINST SNAKE

BITE
Gnanavel R*
Plant Molecular Biology Lab, Department of Botany, Government Arts College,
Udhagamandalam- 643 002. The Nilgiris, Tamil Nadu, India
Abstract
Irulas are a group of primitive tribal population; they were distributed in all the districts
of Tamil Nadu except Ramanathapuram. They practice herbal based treatment for various
ailments including snake bite. But nowadays this traditional practice is eroding duo to the
urbanization and the advent modern medicine. Hence crucial action is needed to protect these
traditional knowledge practices from erosion. Here we made an attempt to elucidate the herbal
antidote against to snake bite.
Keywords: Irulas, Herbal antidote, Snake bite, Tamil Nadu, Tribe.
43
ISP-44
INVITRO-ANTIOXIDANT ACTIVITY & ANTI-INFLAMMATORY ACTIVITY OF

AERIAL PARTS OF ACALYPHA WILKESIANA USING 70% HYDROALCOHOLIC
EXTRACT
E.Vinisha*
Abstract
New drugs/synthetic drugs are often unaffordable to the poor therefore there is a need for
improving & formulation of our surrounding useful medicinal plants into dosage forms for
treatment of diseases. ACALYPHA WILKESIANA different parts is used for medicinal purpose
for the treatment of hypertension, breast tumors, inflammation, post partum pains, cold,
swellings. The 70% hydroalcoholic extract was prepared by using maceration process. The plant
material (aerial parts) was air dried & reduced to coarse powder. The powdered material was
subjected to maceration with petroleum ether for two days for the removal of chlorophyll
content. The dried powdered material was macerated with 70% alcohol for seven days with
frequent shaking. The filtered macerate is distilled & the residue was kept in dessicator till for
further use. The percentage yield of the extract was 11gms. The 70% hydroalcoholic extract of
A.wilkesiana showed the presence of 28µg of flavonoid content by aluminium chloride
calorimetric method. The antioxidant activity of 95.2% of inhibition was observed at 600µg/ml
by DPPH method & the anti-inflammatory activity of 441.4% of protection was observed at
600µg/ml by HRBC membrane stabilization method.
Key Words: Acalypha wilkesiana, inflammation, DPPH method, HRBC membrane stabilization
method.
44
ISP-45
FORMULATION AND CHARACTERIZATION OF GIRARDINIA HETEROPHYLLA
LOADED SOLID LIPID NANOPARTICLES FOR THE TREATMENT OF DIABETES
MELLITUS
*T.L.P chandana, s.karishma
Abstract
Solid lipid nanoparticles (SLNs) are at the forefront of the rapidly developing field of
nanotechnology with several potential applications in drug delivery and research. Girardinia
heterophylla is selected especially for its Anti-diabetic, Anti-microbial, Anti-oxidant, Anti-
inflammatory activities. The objective of present work is to formulate and characterize
Girardinia heterophylla loaded SLNs for diabetes mellitus. Girardinia loaded SLNs were
prepared by high pressure homogenization followed by ultrasonic technique. The particle size
and polydispersity index of optimized SLNs formulation were measured by dynamic light
scattering technique and found to be <200 nm, <0.2 respectively. The entrapment efficiency was
found to be 80%. The shape and surface topography of optimized SLNs was observed by
transmission electron microscopy and found spherical in shape. The in vitro release studies of
SLNs were performed by using dialysis bag method and found to be 75% of drug release. SLNs
provide advantages such as optimum particle size, surface functionality, high drug loading,
potential entrapment of drug and improved drug release profile. Girardinia heterophylla loaded
SLNs could improve the therapeutic efficacy by targeting to specific site and could be a
promising career for Anti-diabetic treatment
Key Words: Girardinia heterophylla, SLNs, Diabetes, In vitro drug release.
45
ISP-46
FORMULATION AND CHARACTERIZATION OF GIRARDINIA HETEROPHYLLA

LOADED SOLID LIPID NANOPARTICLES FOR THE TREATMENT OF DIABETES
MELLITUS
*T.L.P chandana, s.karishma
Abstract
Solid lipid nanoparticles (SLNs) are at the forefront of the rapidly developing field of
nanotechnology with several potential applications in drug delivery and research. Girardinia
heterophylla is selected especially for its Anti-diabetic, Anti-microbial, Anti-oxidant, Anti-
inflammatory activities. The objective of present work is to formulate and characterize
Girardinia heterophylla loaded SLNs for diabetes mellitus. Girardinia loaded SLNs were
prepared by high pressure homogenization followed by ultrasonic technique. The particle size
and polydispersity index of optimized SLNs formulation were measured by dynamic light
scattering technique and found to be <200 nm, <0.2 respectively. The entrapment efficiency was
found to be 80%. The shape and surface topography of optimized SLNs was observed by
transmission electron microscopy and found spherical in shape. The in vitro release studies of
SLNs were performed by using dialysis bag method and found to be 75% of drug release. SLNs
provide advantages such as optimum particle size, surface functionality, high drug loading,
potential entrapment of drug and improved drug release profile. Girardinia heterophylla loaded
SLNs could improve the therapeutic efficacy by targeting to specific site and could be a
promising career for Anti-diabetic treatment
Key Words: Girardinia heterophylla, SLNs, Diabetes, In vitro drug release.
46
ISP-47
The Novel Coumarin analogues as Human Estrogen Receptor Inhibitors
A. Jameera Begam1, S. Jubie1, S.P. Dhanabal2

1
Department of Pharmaceutical chemistry, JSS College of Pharmacy, Ooty.
2
Department of Pharmacognosy & Phytopharmacy, JSS College Of Pharmacy, Ooty.
Tamil Nadu, India.
Abstract
As estrogens are well known to play crucial roles in breast cancer development, much effort has
been devoted to block estrogen formation. Although the current existing drugs used for the
treatment of breast cancer function as an anti-estrogen in the breast, they also function as an
estrogen receptor agonist in the uterus and elevates a woman’s risk of getting uterine cancer.
This would be due to the lack of selective binding and partial agonistic effect of the existing
drugs towards the estrogen receptors. Coumarin and its analogues have shown remarkable
activity against breast cancer cell lines through inhibition against estrogen receptors. The
extension of side chain length of the coumarin nucleus at 3rd and 7th position has shown to
increase the receptor binding affinity. It has been proved that poly unsaturated fatty acids have
beneficial effects in chemotherapy. Keeping in mind the estrogenic receptor inhibitory properties
of coumarin nucleus and anti-breast cancer activity of fatty acids, it is proposed to design and
develop novel pharmacophores containing coumarin and fatty acid scaffold. In-silico docking
studies have been carried out in to the crystal structure of human estrogen receptor [PDB id
2IOG].Totally eighteen compounds which showed good docking results have been taken for
further synthesis. Among them, one of the compound 3-methyl-2-oxo-2h-chromen-7-yl stearate
has been synthesized and characterized by spectral analysis. The compound has been screened
for its anti-breast cancer activity against MCF-7 cell lines by MTT method and the results are
compared with standard tamoxifen. The synthesized compound has shown good CTC50 values
than tamoxifen.
47
ISP-48
PHYTOSOMES - A NOVEL DRUG DELIVERY SYSTEMS FOR

PHYTOCONSTITUENTS
Mohamed Sheik Tharik A1*, Nagaswamy Venkadesh2 and Meyyanathan S.N1
1
Department of pharmaceutical analysis, JSS College of Pharmacy, Ooty.
2
Department of pharmaceutics, JSS College of Pharmacy, Ooty
Abstract
Over the past two decades, considerable efforts have been made on the development of novel
drug delivery systems (NDDS) for plant active and extracts. Phytoconstituents, despite having
excellent bioactivity in vitro, exhibit less or no action in vivo, owing to their poor lipid solubility,
high polarity and improper molecular size which cannot be absorbed by passive diffusion. Lipid
solubility and molecular size are the major limiting factors for a phytoconstituents to cross the
biological membranes and to enter systemic circulation following oral or topical administration.
Some of the phytoconstituents are being destroyed in gastric environment or oral administration.
The term ‘phyto’ or ‘plantero’ refers to plant while ‘some’ means cell like, often also known as
Herbosomes. Phytosomes is a novel approach to deliver phytoconstituents that overcomes the
limitation of traditional drug delivery systems. A covalent complex of phytoconstituents with
phosphatidylcholine (a principle phospholipid of biological membrane) makes them significantly
stable and bioavailable. Phytosomes technology has been effectively used to enhance the better
pharmacokinetic and pharmacodynamics profile of popular extracts including milk thistle,
Ginkgo biloba, grape seed, green tea, hawthorn and ginseng etc.
48
ISP-49
EVALUATION OF SYNERGISTIC ANTHELMINTIC ACTIVITY OF POLYHERBAL

FORMULATION ON PHERITIMA POSTHUMA
Abirami AV *, Rajamanickam V, Sivakumar V, Venakateshan N

Department of Pharmaceutical Chemistry,
Arulmigu Kalasalingam College of Pharmacy, Krishnankoil.
Abstract
Prevalence of worm infestation is high in underdeveloped and developing countries due to poor
sanitation and lack of health education. Our study was aimed to find the individual and
synergestic effect of Ocimum sanctum (Family: Lamiaceae), Coriandrum sativum (Family:
Apiaceae), Pongamia pinnata (Family: Fabaceae), Epipremnumn aureum (Family: Araceae) and
Curcuma longa (Family: Lamiaceae) extract because they have been used traditionally for the
treatment of worm infestation. Further exploring the phytoconstituents present in the herbs of
the polyherbal formulation will aid us in the bioactivity present.Various concentration of
ethanolic extracts of various herbs and the polyherbal formulation were subjected for assessment
of anthelmintic activity in Pheritima posthuma. Time of paralysis and time of death were used as
an evaluation parameter. Piperazine citrate (10 mg/ml) was used as a standard drug.
Phytochemical test revealed the presence of alkaloids, flavanoids, glycosides, carbohydrates,
phenolic compounds and tannins. Concentration dependent anthelmintic effect was observed
with the extract. When 100 mg/ml concentration of ethanolic extracts of the polyherbal
formulation was added it showed paralysis of test worm (earthworm) at 18 minutes and death at
39 minutes. The polyherbal formulation has shown substantial anthelmintic activity using in
vitro model on earthworms which may be attributed to the polyphenols present in the extract.
49
ISP-50
CENTRAL NERVOUS SYSTEM DEPRESSANT ACTION OF PERGULARIA DAEMIA

ON RATS
Priyadharshini.S*, Rajamanickam.V, Sivakumar.V, Venakateshan.N, Jeya ananthi.J

Department of Pharmaceutical Chemistry,
Arulmigu Kalasalingam college of pharmacy, Krishnankoil.
Abstract
Objective: To explore the pharmacological effects on the central nervous system (CNS) of
Chloroform and ethanol extracts of Pergularia daemia. Methodology: Pergularia daemia was
found to be used traditionally for the treatment of central nervous system related disorders. To
evaluate the folkloric activity the plant was extracted using various solvents such as chloroform
and ethanol. The extracts were evaluated for locomotor activity tests indicative of unlikely to
involve a direct action on γ-aminobutyric acid type A (GABA-A) receptors using actophotometer
and also for depressant study using forced swim pool tests. The % inhibition of the locomotor
activity was found using the cutting off the beam of light falling on the photocell using rats. The
CNS depressant activity in forced swim pool test was evaluating by measuring the period of
immobility (passive floating without struggling, making only those movements which were
necessary to keep its head above the surface of water). Results: From the evaluation it was found
that the ethanol extract (200 mg/ml) had better CNS depressant activity when compared to
chloroform extract. Mechanisms that possibly underlie this activity include activation of the
inhibitory GABAergic system. Conclusion: The pharmacological properties of the ethanol
extract of Pergularia daemia studied here opens a promising new avenue of research in the field.
50
ISP-51
DESIGN, SYNTHESIS, CHARACTERIZATION OF NOVEL CARBOLINES AS

ANTINEOPLASTIC AGENT
Arivukkarasi.V1*, M.J.N.Chandrasekar1, Raman Rajesh kumar

1
Department of Pharmaceutical chemistry, JSS College of Pharmacy, Ooty.
2
Department of pharmaceutical biotechnology, JSS College of Pharmacy, Ooty. Tamil
Nadu, India.
Abstract
As cancer causes the second largest mortality rate in the world and affects people of all ages,
including fetus, with the risk increasing with age. The knowledge about the cell cycle mechanism
and pathway in cancer pathogenesis is expanding rapidly with the recent developments of novel
strategies and targets like topoisomerase and Telomerase. A broad spectrum of biological
activity has been reported for several carbolines but effective drugs have not been found yet
among the subclass of carbolines. Various heterocycles like carbozoles, β-carbolines, α-
carbolines and γ-carbolines have been reported with linearly or angularly fused 2,5-
pyrrolidinedione and its derivatives as potential and specific ligand for protein involved in cancer
pathogenesis but δ-carbolines have never been fused with 2,5-pyrrolidinedione or 2-pyrrolidone
due to the lack of expedient methods for their synthesis. Therefore, there is an urgent need for a
novel synthetic strategy for the construction of novel δ-carbolines fused with 2, 5-
pyrrolidinedione. Keeping in mind the receptor inhibitory properties of carboline nucleus and
cancer activity of 2-pyrrolidone, it is proposed to design and develop novel pharmacophores
containing carbolines and 2-pyrrolidone scaffold. In-silico docking studies have been carried out
in to the crystal structure of telomerase. Totally ten compounds which showed good docking
results have been taken for further synthesis. Among them, one of the compound eight has been
synthesized and characterized by spectral analysis. The compound has been screened for its
cancer activity against Hep2, A549, and HCT cell lines by MTT method and the results are
compared with normal Vero cell lines. The synthesized compound has shown good CTC50
values than Vero cell lines.
51
ISP-52
PREPARATION, EVALUATION, WOUND HEALING AND ANTI-MICROBIAL

ACTIVITIES OF HERBAL OINTMENT FROM ETHANOLIC EXTRACT OF
AZADIRACHTA INDICA AND CURCUMA LONGA
V.Veeralakshmi*, 1S.R. Senthilkumar, 1N.Venkateshan, 1V.Rajamanickam, 1R. Ramprasad,

Department of Pharmaceutics, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar,
Krishnankoil -626126, Tamil Nadu, India. Email: sindhukani3007@gmail.com
Abstract
The objective of the study was to formulate and evaluate the wound healing and antimicrobial
activities of herbal ointment from the local medicinal plants. The present work is to formulate
and evaluate the ointment of Neem (Azadirachta indica) and Turmeric (Curcuma longa). The
ethanolic extracts were prepared by using maceration method. The ointment base was prepared
and formulation of ointment was done by incorporating the extract in the base by levigation
method. There are prepared two types of ointment. One ointment contains extract of neem leaf
and turmeric (NLT 5% & 10%) and another one contain extract of neem seed and turmeric (NST
5% & 10%). After completion of formulation it was evaluated for its physicochemical
parameters like colour, odour, pH, spreadability, extrudability, consistency, diffusion study,
solubility, washability. Also the formulation was evaluated for its stability at various temperature
conditions which shows no change in the irritancy, spreadability and diffusion study. The anti-
microbial activity and minimum inhibitory concentration of herbal ointments were investigated
against Pseudomonas aeruginosa, Bacillus subtilis and Escherichia coli. The wound healing
activity was carried out by using wister albino rats. The ointment contains neem seed and
turmeric extract showed maximum antibacterial activity against Pseudomonas aeruginosa,
Bacillus subtilis and Escherichia coli compared to ointment containing neem leaf and turmeric
extract .The observation and results obtained in wound healing study indicated that the ointment
of NST significantly stimulated wound contraction.
52
ISP-53
HEPATOPROTECTIVE ACTIVITY OF LEAVES EXTRACT OF CADABA FRUTICOSA
N.Venkateshan1, P.Thirupathy Kumaresan2, P.Vijay Nagendiran2, M.Subramaniyam2*
1
Department of Pharmaceutical Chemistry
2
Department of Pharmacology
Arulmigu Kalasalingam College of Pharmacy
Anand Nagar, Krishnan Koil
Tamilnadu.
Abstract
The study of hepatoprotective activity of petroleum ether and chloroform extract of leaves of
Cadaba fruticosa at doses of 200 and 400 mg /kg against paracetamol induced hepatotoxicity.
Hepatotoxicity was induced in male albino rats by oral administration of paracetamol (6000
mg/kg for eight days). The toxic effect of paracetamol was controlled significantly by restoration
of the levels of serum billirubin, protein and enzymes as compared to the normal and the
standard drug (silymarin treated groups). The extract of Cadaba fruticosa possesses significant
hepatoprotecive activity.
Keywords
Paracetamol, Cadaba fruticosa, Petrolium ether and chloroform extract.
53
ISP-54
BIOACTIVE FOOD COMPONENT POLYPHENOL AND THEIR HEALTH BENEFITS
Sangari.R*, Jeya Ananthi. J, Venkateshan. N Rajamanickam.V
Arulmigu Kalasalingam College of Pharmacy,
Anand Nagar, Krishnan Koil.626126
Abstract
Nutraceuticals are bioactive, natural chemical compounds which promotes health, preventing
disease & medicinal properties. Functional foods are closely associated with nutraceuticals food
based formulation also contain nutraceuticals. Important bioactive food components are
polyphenols, anthocyanin, carotenoids, flavonoids, limonoids, omega-3 & 6-Fatty acids, and
phytosterols.
They have specific pharmacological effects as Anti-inflammatory, Anti-allergic, Antioxidants,
Hepatoprotective, Neuroprotective & Antidiabetic properties. Polyphenol is a potential
functional ingredient. The major dietary polyphenols are cereals, legumes, oilseeds, fruits,
vegetables & beverage. Every 100 g of fresh apple, grape and pear contains 200-300 mg of
polyphenols. A cup of coffee or tea, a glass of red wine has 100 mg of polyphenols.
Total dietary intake of polyphenols may be 1 gm / day. They are 10 times higher than vitamin –C
& 100 times higher than vitamin-E & carotin. About 8000 structural varieties of polyphenols
(one or more hydroxyl derivatives of benzene) are available. The entire mechanism of action of
polyphenols were not known; but few polyphenols are absorbed as phenolic acids & aldehydes
by the micro flora of the intestine. Some phenols present in dietary phytochemicals (curcumin,
resveratrol, capsaicin, vitamins, beta carotene, curcuminoids & catechins) actively suppress the
inflammatory process, proliferation, apoptosis, redox balance and protective against cancer,
neurodegenerative disorders & cardiovascular disease. The unique property of polyphenol
(genistein, curcuminoids & catechins) is suppressing activity of Akt thus by inhibiting the cancer
cell growth. Most of the dietary polyphenols possess substantial skin photo protective effect. In
recent years there is much awareness about functional foods & nutraceuticals strengthened with
natural polyphenols having health benefits like Anti – carcinogenic, Anti –diabetic, Anti – viral
properties.
54
ISP-55
COMMUNITY PHARMACISTS CENTERED RURAL MOBILE ROLE IN
DIABETIC RETINOPATHY
C. Muthumari*, Nilsha Anil, N.Venkatesan, R. Ramprasad

Arulmigu Kalasalingam College of Pharmacy, Anand nagar, Krishanan koil
Abstract
Diabetic Retinopathy (DR) is considered as one of the leading global causes of blindness. Long-
term poor glucose control leads to vascular incompetency, tissue swelling, new vessels
formation, and if untreated, to irreversible severe visual loss. This study is fully based on survey
modes by asking questionnaire to the community pharmacists about their attitude, activities and
roles in disease management which mainly focuses on Diabetic Retinopathy cases Diabetic
Retinopathy are the most common diseases in all over world which causes co morbid
complications and it leads to increase in mortality rate. Community pharmacy is very much close
to the public and they easily mingle with patients when compare to other health care
professionals. The diabetes Retinopathy patients need chronic medication therapy because of
which they routinely visit their community pharmacies. The community pharmacists are able to
counsel the patients with diabetes Retinopathy. This article emphasis that the assessment shows
the community pharmacist plays an important role in disease management, patient counselling,
creating awareness, reducing the risk of poly pharmacy, self-monitoring on blood glucose and
blood pressure in patients with diabetes Retinopathy.
Key words
Community pharmacy; Patient counselling; Dispensing error; Diabetes; Retinopathy;
55
ISP-56
ANTILIPID PEROXIDATION EFFECT OF
ALANGIUM SALVIFOLIUM SUB. SP. HEXAPETALUM.
Madhumitha K*, Rajamanickam V, Sivakumar V, Venkateshan N

Department of Pharmaceutical chemistry
Arulmigu kalasalingam college of pharmacy

Anand nagar, Krishnankoil-626126
Abstract
Alangium salvifolium sub. Sp. Hexapetalum (Family: Cornaceae) is a commonly used plant for
the treatment of inflammations, rheumatism, haemorrhoid, worm infestations and skin infections.
The root-bark is also used in traditional medicine skin problems and as an antidote for snake bite.
The petroleum ether and methanol extracts of dried roots were subjected to phytochemical
analysis and it revealed the presence of steroids, flavanoids and saponins. The present study was
attempted to explore the in vitro anti-oxidant activity of this plant using DPPH, Super oxide,
nitric oxide free radical scavenging assay and anti-lipid peroxidation methods using goat liver
homogenate. The extracts significantly in a concentration dependent manner produced anti-
oxidant property in all the 4 methods. When compared with ascorbic acid the methanol extract
showed significant anti-oxidant property and free radical scavenging activity. Further
investigation of the extracts may be useful for the invention of lead molecules for ailments
produced by the pathogenesis of free radicals.
Keywords:
Alangium salvifolium, malonaldehyde Anti-oxidant, anti-lipid peroxidation and free radical
scavenging activity.
56
ISP-57
EFFECT OF VITAMINS ON BLOOD GLUCOSE LEVEL IN TYPE 2 DIABETES
PATIENTS
Raja K.*, Sam Johnson Udaya Chander J., Venkatanarayanan R.

RVS College of Pharmaceutical Sciences, Sulur, Coimbatore
Abstract
Vitamin B12 has been used as a treatment for peripheral neuropathy in diabetes and vitamin B6
acts as cofactor for hundreds of enzymes and plays a role in glucose, lipid and amino acid
metabolism and neurotransmitter synthesis. Hence this study is aimed to assess in beneficial of
vitamin B12 on type 2 diabetic patients. To determine effect of vitamins on (metformin induced)
type 2 diabetes patients. To investigate the effectiveness of a daily multinutrient supplements in
diabetes patients. To evaluate the effect of vitamin B12 on metformin induced diabetes. To
evaluate the effect of vitamin C on blood glucose of type 2 diabetes patients. To measure the
prevalence of type 2 diabetes induced by Metformin. Patient underwent treatment with 500mg
vitamin B12 and 40 received 1000mg vitamin C. There were no significant difference in systolic
BP, BMI , FBS, between two groups before treatment, but diastolic BP (p<0.05) were
significantly different. After 6 weeks treatment with nutritional supplement consumption of 1000
mg vitamins resulted in significant changes in the serum level of FBS, but, supplementation of
500 mg vitamin B12 did not result in any changes in this parameters. The study conclude that
1000µg of B12 injection that would provide more than the normal need every patient on long
term Metformin therapy, the study demonstrates that administration of daily multi nutrient
(B6,B9) B12 supplement for 3 months to significant improvements in the well-being of patient
with type 2 diabetes. The study strongly recommends the supplementation with 1000mg/day of
Vitamin C in addition to the normal diet and treatment schedule may help in improving plasma
glucose and lipid profile in patients with T2DM.
57
ISP-58
DESIGN, FABRICATION AND CHARACTERIZATION OF THROMBOLYTIC
ACTIVITY OF BAUHINIA RACEMOSA EXTRACT LOADED NANOEMULSION
Preeyanga K. Akelesh T. Sruthi Ramesh Timane, Sam Johnson Udaya Chander J.,
Venkatanarayanan R.
Abstract
Background: Thrombosis is the formation of an unwanted clot within a blood vessel or heart. It
is the most common abnormality of hemostasis, but it can turn to larger complication causing
disturbance in the normal functioning of many organs. Aim: To evaluate the blood related
properties and thrombolytic activity of Bauhinia racemosa, and to convert it into a nanoemulsion
formulation. Material and Methods: The leaves of the plant were collected from Sulur region,
Coimbatore, India. They were then shade dried and extracted with ethanol. The extract was then
studied for thrombolytic activity and finally designed into a nanoemulsion using Tween 80,
ethanol, cinnamon oil, distilled water in varying ratios of 2:1, 3:1, 4:1. The prepared
nanoemulsion was then evaluated for release study, based on the release the optimized
formulation was selected and further studies like particle size distribution, zeta potential, particle
morphology, stability study were carried out. Results: The thrombolytic activity was carried out
by in-vitro method and gave appreciable results. The extract showed activity close to that of the
standard. The other evaluation parameters of nanoemulsion like particle size, zeta potential,
particle morphology study was done only for the optimized batch. The particle size and zeta
potential reports were good. The stability study shows satisfactory results. Conclusion:
Therefore this study was concluded that the Bauhinia racemosa extract loaded nanoemulsion
drug delivery is effective for the treatment of thrombosis.
58
ISP-59
THE EFFECTIVENESS OF NATURAL PRODUCTS IN INHIBITING P-
GLYCOPROTEIN THEREBY REDUCING MULTIDRUG RESISTANCE TO CANCER
CHEMOTHERAPY –
A SYSTEMATIC REVIEW
Arun Kumar K., Ashwik S., Purushothaman V., J. Sam Johnson Udaya Chander,
Venkatanarayanan R.
Abstract:
Cancer is a leading cause of death worldwide, accounting for 7.6 million deaths (around 13% of
all deaths) in 2008. Carcinogenesis is generally recognized as a multistep process in which a
normal cell acquires specific phenotypic characteristics including self-sufficiency in growth
signals, evasion of apoptosis, limitless replicative potential, sustained angiogenesis and the
ability to undergo tissue invasion and metastasis. Hence, the principle aim of the study is to
review some of the potential herbal drugs which can act as potential P-glycoprotein inhibitor.
There are a lot of studies to say that some natural compounds are effective in inhibiting P-
glycoprotein. Chung S.Y. et al. (2005) proved through his study that some flavonoids such as
biochanin A and silymarin may reverse MDR by inhibiting the P-gp function. Yoshida et al.
(2006) from his study concluded that natural products containing terpenoids may inhibit P-gp-
mediated transport and interact with P-gp substrates in the intestinal absorption process. There
are lot other studies other than these two studying the usefulness of herbs in inhibiting P-
glycoprotein. Hence this review concludes that herbal drugs may be a potent alternative to
formulate a p-glycoprotein inhibitor in the future thereby reducing multidrug resistance in
chemotherapy.
59
ISP-60
FORMULATION AND CLINICAL EVALUATION OF HEPATOPROTECTIVE
POLYHERBAL FORMULATION-A PHASE II CLINICAL TRIAL
Nazzneen MV., C.S. Kandasamy, R. Venkatanarayanan

Abstract
Background: Diseases are destroyers of health, well-being, and life. So, Arogya is the main
constituent for excelling in life. Jaundice is one disease which has manifested itself as a curse for
many years. Even today, there is no good allopathic medicine for treatment and complete cure of
jaundice. This study aims to make a formulation for the cure of jaundice by combining various
herbals to cure jaundice. Methods: Based on the ethnomedical literature reviews, the crude drugs
such as cardamom, ajowan, cinnamon, coriander, cumin, clove, ginger, and nutmeg were
collected and subjected to various pharmacognostical, phytochemical, and analytical studies to
assess it quality. After confirming the quality, the hepatoprotective polyherbal formulation
(RVSPHF123) was formulated and their efficacy was analysed using various analytical and
pharmacological studies. To assess the safety of RVSPHF123, clinical trials were conducted
using this formulation at Ayushkaram Ayurveda Hospital, Coimbatore after obtaining Human
Ethical Clearance approval by IEC, Institute of Applied Dermatology, Kasaragod. Results: The
present study has been carried out in 30 patients. All the patients were given RVSPHF123 at a
dose of 60 ml three times a day one hour after food. RVSPHF123 significantly improved the
liver profile of the patients with reduced SGPT, SGOT, SALP, Total Bilirubin, Total Cholesterol
and significant improvements in Total Protein, Albumin profiles in liver damage patients with a
P value of 0.001, which is also statistically significant. Conclusion: RVSPHF123 has proved its
efficacy in the management of jaundice.
60
ISP-61
AN UPDATED REVIEW ON BAUHINIA RACEMOSA LAM. AND ITS MEDICINAL
PROPERTIES
Shafna KK., Pavithra R., Akelesh T., Chitra Jose., Sam Johnson Udaya Chander J., Kandasamy
CS., Venkatanarayanan R.
Abstract
The aim of the present study is to extensively review the phytochemical and pharmacological
activities of Bauhinia racemosa, belonging to family fabaceae. Phytochemical screening of the
dried leaf extract showed the presence of proteins, carbohydrates, flavonoids, alkaloids, amino
acids, glycosides, triterpenoids, and tannins. The ethanolic extract of Bauhinia racemosa
exhibited various pharmacological activity including Antimicrobial, Antioxidant, Anti HIV-1,
Antifilarial, Antihelmintic. Antihistaminic, Analgesic, Antiulcer, Anticancer, antipyretic, anti-
inflammatory, hepatoprotective, and Astrigent properties.
Keywords Bauhinia racemosa, fabaceae, Phytochemical.
61
ISP-62
JNC 7 VERSUS JNC 8 – UNDERSTANDING THE EVIDENCES
Ammu M., Pradeepa M., Sharaniya M., Sam Johnson Udaya Chander J., Venkatanarayanan R.
Abstract
Hypertension is growing as a global burden now. It is essential to provide a standard and
harmonized guideline for hypertension treatment. There is strong evidence to support treating
hypertensive persons aged 60 years or older to a BP goal of less than 150/90 mmHg, however,
there is insufficient evidence in hypertensive persons younger than 60 years for a systolic goal,
or in those younger than 30 years for a diastolic goal, so the panel of Eighth Joint National
Committee (JNC 8) recommends a BP of less than 140/90 mmHg for those groups based on
expert opinion. The same thresholds and goals are recommended for hypertensive adults with
diabetes or nondiabetic chronic kidney disease (CKD) as for the general hypertensive population
younger than 60 years. There is moderate evidence to support initiating drug treatment with an
angiotensin-converting enzyme inhibitor, angiotensin receptor blocker, calcium channel blocker,
or Thiazide-type diuretic in the nonblack hypertensive population, including those with diabetes.
In the black hypertensive population, including those with diabetes, a calcium channel blocker or
Thiazide-type diuretic is recommended as initial therapy. There is moderate evidence to support
initial or add-on antihypertensive therapy with an angiotensin-converting enzyme inhibitor or
angiotensin receptor blocker in persons with CKD to improve kidney outcomes. This educational
material debates the recommendations and limitations of JNC 8 Guidelines in the management of
hypertension.
Keywords JNC 8 Guidelines, Chronic Kidney Disease, Diabetes mellitus, diuretic, angiotensin-
converting enzyme inhibitor, angiotensin receptor blocker.
62
ISP-63
SCIENTIFIC EVIDENCE FOR TRADITIONAL CLAIM OF ANTI-LITHIATIC
POTENTIAL OF DIFFERENT MUSA SPECIES
E.Ram Kumar*, S.S.Rajendran, Santhi, N, Sivapriya.V, Mohammed Iqbal, Venkatanarayanan R.
Department of Pharmaceutical Chemistry, RVS College of Pharmaceutical Sciences,

Sulur, Coimbatore, Tamil nadu 641402, India.
Abstract
Objective: Kidney stone is one of the most prevalent diseases worldwide and calcium oxalate
has been shown to be the main component of the majority of stones formed in the urinary system
of the patients. The aim of the present research the comparative study on in-vitro anti-lithiatic
potential of different Musa (Banana) species of stems by turbiditory method. Methods: A freshly
collected different musa species (M1, M2, M3, M4). A total of 200 g stems from each musa
species was extracted with 500 ml water at room temperature. The extracts were then filtered
through filter. Microscopic study was done for the comparative evaluation of crystal density and
size in each treatment in turbidity method. Results: In all he treatments concentration dependent
initial steep rising in turbidity (nucleation) followed by decrease turbidity (aggregation) was
seen. Maximum inhibition 60.19% in stone nucleus formation (turbidity) was seen by the
aqueous extract of the M4(Aqueous extract of Poovan stem) stem after180 seconds of chemical
reaction started as compared to the control significantly different from other extract. M3
(Aqueous extract of Rasthali stem) got second position among plant leaves extracts in
bioactivity test which inhibited 47.96% of stone nucleus (turbidity) formation, followed by
M1(Aqueous extract of Sevvalai stem) extract which inhibited 22.13 %in turbidity and are
significantly different from each other. Least inhibition in nucleation was seen by M2 (Aqueous
extract of kunnan stem) extract which accounted only 13.98 % inhibition. The control drug
Cystone inhibited 81.21% in stone nucleation. Conclusion: The exiting fact came out of the
study is that the aqueous extract of different musa species stem potential compared to standard
drug cystone in dissolving the artificial stone crystals.
63
ISP-64
EXTRACTION AND ANTIBACTERIAL SCREENING OF MIXTURE OF ACALYPHA
INDICA LINN AND TRIDAX PROCUMBENS LINN
*Arulraj, P, Bavatharini, A, Manikandan, V,
Sam Johnson Udaya Chander, J, Kandasamy C S, Venkatanarayanan, R.
Abstract
Aim: In the present investigation antibacterial of ethanolic extract of mixture of the Acalypha
indica and Tridax procumbens were studied. Process: The antibacterial activity was assessed by
cup and plate method and filter paper disc method by using MullerHinton agar medium. The
zone of inhibition was compared with each other concentrates of mixture of Acalypha indica and
Tridax procumbens. The present investigation says that, as the concentration increases the zone
of inhibition also increases. The antibacterial activity of the mixtures of Acalypha indica and
Tridax procumbens, alcoholic extract was assessed by using cup and plate method. Results: The
2mg/ml of the mixture produce 3.5mm in Staphylococcus aureus and E.Coli. The 4mg/ml of the
mixture produce 4mm in Staphylococcus aureus and 4.5mm of E.Coli. 8mg/ml of the mixture
produce 9.5mm of Staphylococcus aureus and 10.5mm of E.Coli. Hence the above finding says
that 8mg/ml mixture of Acalypha indica and Tridax procumbens plant extract shows good
activity. The antibacterial activity of the mixtures of Acalypha indica and Tridax procumbens,
alcoholic extract was assessed by using filter paper disc method. The 2mg/ml of the mixture
produce 3.5mm in Staphylococcus aureus and E.Coli. The 4mg/ml of the mixture produce 4mm
in Staphylococcus aureus and 4.5mm of E.Coli. 8mg/ml of the mixture produce 10.5mm of
Staphylococcus aureus and 9.5mm of E.Coli. Hence the above findings says that 8mg/ml mixture
of Acalypha indica and Tridax procumbens plant extract shows good activity. Conclusion: The
findings confirm that the traditional therapeutic claims for this plant, in near future surely be able
to replace the conventional anti-bacterial agents to which there is increased incidence of drug
interactions and the study suggests that this plant is promising for development of phytomedicine
for antibacterial properties.
Keywords:
Acalypha indica, Tridax procumbens, antibacterial, ethanolic extract
64
ISP-65
ANTIBACTERIAL OF ETHANOLIC EXTRACT OF MIMOSA PUDICA LEAVES
Rubasri.R*, Dhanalakshmi, Arulraj, P., Bavatharini, A., Manikandan, V.,

Sam Johnson Udaya Chander, J., Kandasamy C S., Venkatanarayanan, R.
Department of Pharmaceutics including Pharmaceutical Biotechnology,

R.V.S College of Pharmaceutical Sciences,
Sulur, Coimbatore – 641402
Abstract
Aim: In the present investigation is antibacterial of ethanolic extract of the Mimosa pudica was
studied. The antibacterial activity was assessed by agar well diffusion method by using
MullerHinton agar medium. The zone of inhibition was compared with the standard antibiotic
Gentamycin. Method: 8mg/ml concentrated Mimosa pudica plant extract shows near activity to
the standard drug Gentamycin. The findings confirm that the traditional therapeutic claims for
this plant, in near future surely be able to replace the conventional anti-bacterial agents to which
there is increased incidence of drug interactions and the study suggests that this plant is
promising for development of phytomedicine for antibacterial properties. Results: Mimosa
pudica alcoholic extract is shows the anti-bacterial activity, and for the dilution of 2, 4, 6,
8mg/ml the zone of inhibition was found to be the length of 3.5, 4, 6.5, 10.5 mm respectively in
Streptococcus (Fig.4). For the dilution 2, 4, 6, 8mg/ml the zone of inhibition was found to be the
length of 3.5, 4.5, 7, 9.5 millimeter respectively in E.Coli (Fig.5). For 2, 4, 6, 8 mg/ml dilutions
the zone of inhibition is found to be 4, 4.5, 7.5, 10, 14.5 mm respectively in Klebsilla.
Conclusion: The findings confirm that the traditional therapeutic claims for this plant, near
future surely be able to replace the conventional anti-bacterial agents to which there is increased
incidence of drug interactions and the study suggests that this plant is promising for development
of phytomedicine for antibacterial properties.
65
ISP-66
IN VIVO WOUND HEALING PROPERTY OF TRADITIONAL MEDICINAL PLANT
Lavanya. N *, Jagdeesh S., Kandasamy C., Akshal Devakirubhai.J., Ashwini.M.,

Venkatanarayanan R.
Abstract
Objective: To pre-screen the ex- vivo wound healing activity of flavonoid rich fraction of Ethyl
acetate extract of the leaves of Trichosanthes cucumerina Linn. Family Cucurbitaceae using
porcine skin wound healing model (PSWHM) along with phytochemical, EDS, HPTLC analysis.
The aim of this present study is to provide pharmacological validation to the traditional claim for
wound healing activity of Trichosanthes cucumerina leaves.
Keywords:
Trichosanthes cucumerina, Cucurbitaceae, Epidermal migration, ex-vivo wound healing, Trace

element, Energy Dispersive X-ray Spectrometer, HPTLC
66
ISP-67
CARDIO PROTECTIVE EFFECT OF THE LEAVES OF TRICHOSANTHES

CUCUMERINA L. ON DAPHNIA MAGNA
Priyadhrshini.M *, Deepika.V , Khokila.D *, Jagdeesh.S., Kandasamy. C. S., Akshal

Devakirubhai.J., Ashwini.M., Venkatanarayanan, R.

Abstract
Objective: To pre-screen the in vivo cardioprotective activity of the leaves of Trichosanthes
cucumerina.L Family Cucurbitaceae using the model organism Daphnia magna along with
preliminary phytochemical study and acute toxicity assessment. Method: To evaluate the
protective effect of the ethyl acetate extract of the leaves of T.cucumerina (TCEAE) in vivo on
the lactose induced arrhythmic heart of the cladocerans D.magna (Water flea) a novel model
system for studying effects of agonists and toxins on cell signalling and ion channels in situ.
Initially acute toxicity assessment, total phenolic content by UV spectral methods and Apigenin
content by HPTLC, trace elements by X-ray fluorescence were determined. Results: Normal
mean heartbeat of the D.magna at 20±20 C was found to be 190.47±0.54 beats/min (n=50).
Arrhythmia was induced by lactose (200mM) in the bathing medium. The ethyl acetate extract of
the leaves of T.cucumerina (20, 40, 60, 80µg/ml) prevented the lactose induced arrhythmia in
dose dependent manner. Previous assessment of toxicity showed LC50 6.31mg/L. Total
phenolic, apigenin content of TCEAE was 376.5mg/g, 0.77% respectively. High percentage of
Calcium (5.84%), Potassium (5.91%), Magnesium (0.5%), Phosphorous (1.04%), Silica (0.35%)
was found in T.cucumerina leaves. Conclusion: Trichosanthes cucumerina L. (Pudal leaf) has
long been recognized and economically is of appreciable importance as a source of edible
aggregate fruit. This study indicates that the TCEAE possesses potential cardiac protective
activity on the lactose induced arrhythmia of the Daphnia heart without any toxicity and
mortality. It is assumed that this may be due to polyphenolic content, apigenin, trace elements
like calcium, potassium, magnesium, phosphorous, silica. Further investigation requires
confirming this activity.
Key words
Trichosanthes cucumerina, Cucurbitaceae, Cardioprotective, Daphnia magna, Apigenin.
67
ISP-68
GLP-1 MEDIATED ANTI-AZ ACTION CURCUMA LONGA CONSTITUENTS – AN

INSILICO, INVITRO AND INVIVO ANALYSIS
Nehru Sai Suresh Chalichem*1, Duraiswamy Basavan1, Damodaran.P1, Srikanth Jupudi2

1 Dept. of Pharmacognosy & Phytopharmacy, JSS College of Pharmacy, Ootacamund
2 Dept. of Pharmaceutical Chemistry, JSS College of Pharmacy, Ootacamund
, Tamilnadu – 643001
Abstract
Dipeptidyl peptidase IV (DPP-4) inhibitors are a new class of oral anti-hyperglycemic agents for
the treatment of type 2 diabetes. The glucose lowering effect of DPP-4 inhibitors is mediated by
suppressing the degradation of the incretin hormone glucagon-like peptide-1. After the discovery
of neuroprotective action of GLP-1, it has been targeted for neurodegenerative disorders.
In the present study, the In-Silico DPP-4 inhibitory activity was analyzed for the
phytoconstituents reported in Curcuma longa plant. The binding affinity of the phytochemicals
from the above mentioned plants were calculated by performing the docking experiment using
Glide. The molecule (CA) that stands next to curcumin was subjected for invitro analysis and
further Insilico analysis like free energy calculations and dynamic studies. As the molecule has
shown considerable inhibitory activity it was subjected for invivo analysis.
Therefore, in the present study, the neuroprotective role of CA for ameliorating the
streptozotocin (STZ) induced AD have been tested in rat model. Experimentally, CA at a dose of
200 mg/kg, was administered orally to STZ induced AD Wistar rats and cognitive evaluation
tests were performed using radial arm maze and hole-board apparatus. Following 30 days of
treatment with the compound, attenuation of AD pathology, as evidenced by decreasing amyloid
beta 42, total tau, phosphorylated tau and neuro-inflammation with an increase in glucagon-like
peptide-1 (GLP-1) levels was observed.
68
ISP-69
EFFECT OF HERBAL EXTRACTS ON PARAOXONASE ENZYME: IN VITRO
SCREENING AND IN SILICO DOCKING APPROACH
B.Vanitha* and Dr. B. Duraiswamy

Department of Pharmacognosy and Phytopharmacy, JSS College of Pharmacy, Ooty
Abstract
Paraoxonase 1 (PON1) is an enzyme that is mainly synthesised in the liver and protects LDL
from oxidation, thereby exhibiting antiatherogenic properties. Medicinal plants play a major role
in controlling the lipid levels as anti-atherosclerotic agents. The ethno botanical reports of the
herbs Bambusa arundinacae (Graminae), Cajanus cajan (Fabaceae) and Diospyros kaki
(Ebenaceae) have been traditionally used for many medicinal purposes. These plants are reported
to be beneficial in wide range of biological and therapeutic uses including anti-oxidant, anti-
inflammatory, anti-hyperlipidemic etc. The present study was carried out to evaluate in vitro
paraoxonase activity of the selected medicinal plants and insilico docking studies of the isolated
compounds. The cytotoxicity evaluation study of ethanolic extract and the isolated compounds
indicates that they are nontoxic. The cell secreted paraoxonase activity revealed that the
ethanolic extract of the Diospyros kaki, Cajanas cajan and the isolated compound increased the
biologically active PON1 from the hepatocytes. From the in silico data it is clear that betulinic
acid demonstrates better results than that of cetyl palmitate. Thus it is deduced that the in silico
docking results of betulinic acid and cetyl palmitate are in concurrence with the in vitro cell-
secreted PON1 paraoxonase activity of the molecules.
69
ISP-70
EVALUATION OF ANTIMICROBIAL ACTIVITY OF DELONIX ELATA

Deepak Kumar*
Department of Pharmacognosy, Ranchi Collage of Pharmacy, Ranchi, Jharkhand., India.
Abstract
To maintain human health for the long period of time medicinal plant has played very
precious role. The aim to study antimicrobial activity of Delonix elata. This is important
significant activity because synthetic products have side effects as well as microbial resistance.
Researcher found interest in developing such new and natural antimicrobial agents which
combat microbial resistance. The ethanolic and Aqueous extract of Delonix elata were evaluated
antimicrobial activity against microbes. Agar well diffusion and dish diffusion method were
performed. The 400µg/ml ethanol extract showed minimum inhibition effect as compared
aqueous extract against bacteria as well as fungal pathogens. It is a natural weapon to control
microbes.
Keywords
Antimicrobial activity, Delonix elata, Medicinal plant.
70
ISP-71
FORMULATION AND EVALUATION OF GUAR GUM COMPRESSION COATED
TABLETS OF MEBEVERINE HYDROCHLORIDE FOR COLON DELIVERY
S.Karishma*
Abstract
The present study was to formulate mebeverine HCl colon targeted compression coated tablets
using guar gum to improve the therapeutic efficacy by increasing drug levels in colon. Direct
compression method was used to prepare mebeverine HCl core tablets, and they were
compression coated with guar gum. All the precompression parameters of Mebeverine HCl
tablets were evaluated and found all parameters within the limits. The post compression
parameters of mebeverine HCl compressed coated tablets were evaluated. The weight variation
of the tablets was in the range from 369.05 ± 0.71mg to 348.86 ± 1.17mg. The hardness of the
tablets was found in the range from 6.01 ± 0.67 kg/cm2 to 5.20 ± 0.48 kg/cm2. The core tablets
were compressed coated by using different concentration of guargum.The drug release from the
guargum compressed coated formulations showed less than 6% of drug release at the end of 2hrs
in simulated gastric fluid (0.1N HCl) and the drug release was gradually increased after 3hrs and
it was found to be 65.156 % at the end of 12hrs in simulated colonic fluid (pH-6.8). The drug
release from these tablets showed zero-order profile, and the drug release mechanism was
supercase-II transport. Thus development of guar gum compression coated tablet was a good
approach for colon targeting of Mebeverine HCl.
Keywords
Mebeverine HCl, Guar gum, Colon delivery, Tablet
71
ISP-72
EFFECTIVENESS OF ANTIBIOTICS IN INCREASING THE FUNCTIONAL
CAPACITY AND REDUCING THE ECONOMIC BURDEN IN FEMALE URINARY
TRACT INFECTION PATIENTS
Deepika.V*, Achu.M.Varghese., Anusree.B.J., Sam Johnson Udaya Chander J, Kandasamy CS,

Arulraj P, Venkatanarayanan R.
RVS College of Pharmaceutical Sciences, Sulur, Coimbatore 641402.
Abstract
Background: Urinary tract infection is a common medical problem for women throughout their
lifetime. Recurrence after urinary tract infection (rUTI) is common in adult women. The majority
of recurrences are believed to be reinfection from extra urinary sources such as rectum or vagina.
The treatment of recurrent urinary tract infection also inflicts an economic burden on the
patient’s life. Hence, we must use an effective antibiotic that prevents the recurrence of UTI and
improve the functional capacity and quality of life in women. This study aims to study the
effectiveness of different antibiotics in increasing the functional capacity and reducing the
economic burden in UTI women. Methodology: This is a prospective, observational study,
conducted in a total of 50 patients at General Medicine Department of a tertiary care hospital in
Coimbatore for a period of six months. The patient demographic details were collected from the
medical records or direct patient interview. Urine analysis, urine culture and antibiotic sensitivity
reports were also collected and entered in the data collection form. The functional capacity and
quality of life scores before and after antibiotic administration were calculated using the
Leicester Impact scale. Results: The study shows that the urinary tract infection mainly affects
women between the ages 55 and 65 yrs. Escherichia coli is the principal microorganism that
causes urinary tract infection the most and the major risk factor is menopause (24%) and the least
risk factor is urosepsis (10%). Mostly prescribed antibiotic is combination of Cefoperazone and
Sulbactam and its recurrence rate is 4%. Conclusion: From the study, it is concluded that
amikacin is the most cost effective medication with no recurrence rate and with a mean number
of days of hospital stay of four days.
Keywords
Urinary tract infection, asymptomatic bacteriuria, Antibiotics and functional capacity.
72
ISP-73
ANTI-ALZEIMER’S ACTIVITY OF ETHANOLIC LEAF EXTRACT OF PERSEA
AMERICANA ON THE SCOPOLAMINE-INDUCED RAT MODELS
Kokhila.D*.,Indhu.P., Sam Johnson Udaya Chander J, Senthil Kumar, Pradeepsintha TR,

Manavalan R, Venkatanarayanan
Abstract
Objective: The aim is to determine the presence of total polyphenolic content in leaf extract of
Persea americana plant to evaluate the effect on Alzheimer’s disease by using the Wistar albino
rat. Materials and Methods: A fresh leaves of Persea americana were dried under shadow and
aqueous extract was obtained using soxhlet apparatus. The animals were divided into five groups
of six rats each. The Alzheimer’s disease was induced by using scopolamine (1mg/kg, i.p).
Elevated plus maze model, Radial arm maze model and Hebb’s Williams maze model were
employed to test learning and memory in rats. Two doses (200 mg/kg and 400 mg/kg p.o) of
ethanolic extract of Persea americana were administered for eight successive days in separate
group of animals and donepezil was used as a standard drug (5mg/kg, p.o). Result: The dose of
200 mg/kg and 400 mg/kg p.o of ethanolic extract of Persea americana leaves showed
significantly improved learning and memory of rats in dose-dependent manner compared with
standard drug donepezil (5mg/kg,p.o). Conclusion: The study implied the presence of
polyphenols in Persea americana leaves which is responsible for anti-Alzheimer’s activity
(proved by in-vivo models). The polyphenols are known to be the potential source of
Acetylecholinesterase inhibitor.
Keywords
Alzheimer’s, Persea americana, Polyphenols, Learning and Memory.
73
ISP-74
NUTRITIVE ASSESSMENT ON AN EDIBLE FRUIT OF RUDRAKSHA; A REVIEW
Ragunathan M.*
*Institute of Pharmacy, JJT University, Jhunjhunu, Rajasthan, India
ragunathranilmonica@gmail.com 9865656279 Mobile
Abstract
Elaeocarpus oblongus (Bikki) wildly grown tree Western-Ghats, the ethnic peoples of the
Nilgris district of Tamilnadu state, India are used the fruits for curative purpose. Fruits are
edible; they were extremely cheapest resource for the control of rheumatism and body ache. Fruit
containing seed is a kind of Rudraksha according Hindu mythology. Native people of Nilgiris
district knew about the medicinal and nutritional values of the fruit. Objective: The current
study indented to estimate the fat and water soluble vitamins, phenolics and flavanoids contents
in the fruit pulp of Elaeocarpus oblongus (Elaeocarpaceae) by using HPLC methods. Qian H,
Sheng M. (1998), suggested HPLC methods for analysing the nutritive values of natural
products. Results: The results shown the vitamins A, D3 and E and K identified. Of these, A
(Retinol Acetate) 1.817 mg/ gm, occurred in higher concentration followed by Vitamin K. 0.748
mg/ gm, Alpha -Tocopherol (E) 0.033 mg/ gm, Cholecalciferol, Vitamin (D3) 0.008 mg/ gm was
found comparatively small level. B complex vitamins detected were niacin, pyridoxine,
thiamine, and riboflavin. Amongst these, the highest quantity of water-soluble vitamins in the
fruit pulp were Thiamine 0.334, followed by Riboflavin 0.012, Pyridoxine 0.007, and Niacin
0.002 mgs/gms respectively. Among the Phenolic acids, Gallic acid content was found more in
amount 0.288 mg/ gm, followed by Ferulic-acid 0.009 mg/ gm, Caffeic acid 0.001 mg/ gm,
Quercetin the plant flavanoid present in fruit pulp was 0.012 mg/gm, while all other flavonoids
Rutin was 0.005 mg/ gm, are also in detectable amount. Conclusion: All these essential nutrients
have played an important role in the day to day life of every human. The current study will be
planned to be carried out elaborate investigations of phytochemicals and performing Anti-
nociceptive activity as well.
74
ISP-75
EFFECT OF SALINITY, pH, LIGHT INTENSITY ON GROWTH AND LIPID
PRODUCTION OF MICROALGAE
Vivek Gahanan*
Dept. of Pharmacognosy, JSS College of Pharmacy, Mysuru
Abstract
Microalgae are microscopic unicellular organisms capable to convert solar energy to chemical
energy via photosynthesis. They contain numerous bioactive compounds that can be harnessed
for commercial use. The potential of microalgal photosynthesis for the production of valuable
compounds or for energetic use is widely recognized due to their more efficient utilization of
sunlight energy as compared with higher plants. Microalgae can be used to produce a wide range
of metabolites such as proteins, lipids, carbohydrates, carotenoids or vitamins for health, food
and feed additives, cosmetics and for energy production. Nowadays, there are numerous
commercial applications of microalgae such as microalgae can be used to enhance the nutritional
value of food and animal feed owing to their chemical composition; they play a crucial role in
aquaculture and they can be incorporated into cosmetics. Moreover, they are cultivated as a
source of highly valuable molecules. For example, polyunsaturated fatty acid oils are added to
infant formulas and nutritional supplements and pigments are important as natural dyes. The
important growth parameters like pH, light colour, light intensity and photoperiod were studied
for better production of biomass. The effect of salinity on cell growth was also studied and
compared with normal Fogg’s medium grown cells. The main biomolecules like carbohydrate,
protein, lipid and chlorophyll content were also estimated with the help of standard biochemical
methods in salt supplemented and without salt Fogg’s medium. The cellular lipid content was
increased by growing the cells under different salt concentrations.
75
ISP-76
ELUCIDATION OF MOLECULAR MECHANISMS LEADING TO COLORECTAL
CARCINOMA CELLS DEATH BY TRICAPROIN ISOLATED FROM SIMAROUBA
GLAUCA VIA APOPTOSIS
Asha Jose*1, MVNL Chaitanya2, A. Selvaraj3, Kannan Elango4$, SubbaRao V.
Madhunapantula5$
Abstract
Simarouba glauca, also known as Laxmitaru, is known to contain compounds such as alkaloids
and triterpenes that inhibit the growth of cancers. Recent studies have ascribed the anti-cancer
activity even for lipids. Tricaproin (TCN) is one such lipid exhibiting anti-tumor activity.
However to date no study reported the isolation and characterization of tricaproin. Therefore, a
method was developed to isolate TCN from Simarouba glauca and the isolated molecule
characterized for its structure using NMR, FT-IR and GC-MS. The pure molecule was used to;
first, test its potency for inhibiting the growth of CRC cell lines. Next, the molecular
mechanisms leading CRC cells death was determined by measuring the levels of apoptosis,
reactive oxygen species and HDAC activity. The data demonstrated that the developed
procedure yielded a TCN molecule with >90% purity. In vitro, TCN induced ROS generation
and apoptosis and, inhibited histone deacetylases (HDACs) to retard the proliferation of CRC
cells. In silico, TCN showed better binding to HDACs indicating that TCN could be a potent
inhibitor of oncogenic HDACs. Currently further studies are underway to elucidate the
additional molecular mechanism responsible for TCN induced cancer cell growth inhibition.
76
ISP-77
LIPID MEDIATED BRAIN TARGETED DRUG DELIVERY SYSTEM
CONTAINING ANTI PARKINSON’S DRUG
Sivakumar Kannan*and Jawahar Natrajan

Department of Pharmaceutics, JSS College of pharmacy, Ooty, India-643001
Abstract
The project establishes that dopamine receptor agonists, bromocriptine can be produced as solid-
lipid nanoparticles using glyceryltrimyristate to treat parkinson’s more effectively. This drug
belongs to the category of BCS class II drugs which are basically classified as low solubility and
high permeability drugs. The primary plan of the study was to develop the drug loaded as solid-
lipid nanoparticles by Microemulsion technique. Characterization of the SLN was done by
determining the zeta potential, particle size and polydispersity index, where the optimized
formulation was found to possess a zeta potential of -1.10 mV and particle size and PDI of 68.83
nm and 1.000, respectively. Surface morphology of the SLN was investigated by Scanning
Electron Microscopy (TEM), where the particles were found to be spherical in shape and have a
particle size under 100 nm. Encapsulation efficiency of the optimized SLN was found to be 85%.
From the in vitrorelease (Diffusion) study which was conducted, it was found that the optimized
SLN showed sustained release of drug in comparison with pure drug. The best fitted model gives
the highest R2 value and least slope value. Thus, korsemayer-peppas model fits best for the
dissolution data of the optimized batch as it showed the highest value for R2. (0.968).
Cytotoxicity Studies (Cell-line study) of nanoparticles and placebo were haemocompatible and
did not produce any toxic effects. In-vivo study (Brain bioavailibilty in animals) Brain
distribution studies indicate that there was more than 3-fold increase SLNS formulation
concentration in Brain and neuroprotectivity in case of Glyceryltrimyristate-SLN compared to
Bromocriptine suspension.
Keywords
Bromocriptine, Glyceryltrimyristate, Solid-Lipid Nanoparticles, Microemulsion
77
ISP-78
CLINICAL TRIAL OF HERBAL MEDICINE
Anjana B*
Pharmaceutical Regulatory Affairs, Dept. of Pharmaceutics, JSS College of Pharmacy,
Jagadguru Sri Shivarathreeshwara University, S S Nagar, Mysuru-570015
Abstract
Herbal medicines are finished labelled medicinal product that contain an active ingredient, aerial,
or underground parts of the plant or other plant material or combinations. Herbal derived
remedies need a powerful and deep assessment of their pharmacological qualities and safety that
actually can be realized by new biologic technologies like pharmacogenomics, metabolomics.
Because of the large and growing use of natural derived substances in all over the world, it is not
wise to rely also on the tradition or supposed millenarian beliefs; explanatory and pragmatic
studies are useful and should be considered complementary in the acquisition of reliable data
both for health caregiver and patients.
In 1993, the guidelines for the safety and efficacy of herbal medicines developed by an expert
committee directed that the procedures laid down by the office of the Drug Controller General of
India for allopathic drugs should be followed for all traditional and herbal products to enter into
clinical trials for any therapeutic condition.
Considering the widespread use and popularity of herbal products the need for adequate evidence
on the effectiveness and safety of herbals is mandatory. Clinical evaluation of herbal medicines
is an ongoing process and should include adequate knowledge and understanding of values and
limitations of clinical research, particularly randomized controlled clinical trials.
78
ISP-79
HALLENGES IN THE REGULATION OF TRADITIONAL MEDICINE – A REVIEW

OF GLOBAL SCENARIO
Raveena Nair*
Pharmaceutical Regulatory Affairs, Dept. of Pharmaceutics, JSS College of Pharmacy,
Jagadguru Sri Shivarathreeshwara University, S S Nagar, Mysuru-570015
Abstract
Traditional medical knowledge and practices are widely prevalent around the world since
centuries. With rapidly increasing use of traditional medicine and complementary/alternative
medicine (TM/CAM) throughout the world its safety, efficacy and quality became major
challenge and has made their regulation an urgent need. The effort to regulate TM is a priority of
all governments, which got greater impetus since WHO Declaration of Alma-Ata in 1978.
Though there are various guidelines and international consensus, countries face difficulty in the
development and implementation of the regulation due to its diversity and complexity. The
growth of the pharmaceutical industry and the unceasing development of new and more effective
synthetic and biological medicinal products has not diminished the importance of medicinal
plants in many societies. On the contrary, population growth in the developing world and
increasing interest in the industrialized nations have greatly expanded the demand for medicinal
plants themselves and the products derived from them. Regulations in countries for the
assessment of the quality, safety and efficacy of medicinal plants, and the work of WHO in
supporting the preparation of model guidelines in this field, have been helpful in strengthening
recognition of their role in health care. It is hoped that assessment of these traditional remedies
could become the basis for a future classification of herbal medicines, as well as for evaluative
studies on their efficacy and safety, and their potential use in national health care systems in
different parts of the world.
79
ISP-80
NOVEL 9-SUBSTITUTED β-CARBOLINE ANALOGUES: A SELECTIVE POLO LIKE

KINASE-1 INHIBITORS IN CANCER BIOLOGY
Gomathi Priya Jeyapal*1, Chandrasekar MJN1, Rajendiran Krishnasamy2.

1
Dept. of Pharmaceutical Chemistry, 2Dept. of Pharmacognosy and Photochemistry
(Off campus-JSS University, Mysore)
JSS College of Pharmacy, Ootacamund-643001.
Abstract
The development of potent and selective kinase inhibitors is a challenging task in modern drug
discovery of anticancer drugs. The innate promiscuity of kinase inhibitors largely results from
ATP-mimetic binding to the kinase hinge region. We present a novel class of 9-substituted-β-
carbolines whose kinase inhibitory activity does not rely on canonical ATP-mimetic hinge
interactions. A library of 200 carboline analogues was designed and their in-silico docking
studies were performed on various targets. The docking results showed an good binding affinity
towards the PLK-1 kinase. All the designed compounds exhibited good binding interactions with
the active site residues of different targets. Therefore, we suggest that this scaffold may serve as
a valuable template for the design and development of specific kinase inhibitors of cancer
pathology.
Keywords
Carbolines, Cancer, Docking, Kinase inhibitor.
80
ISP-81
PROMOTION OF CERVICAL CANCER CELL DEATH BY SUPPLEMENTATION OF

ANTIOXIDANT VIA OXIDATIVE STRESS INDUCED DOWN REGULATION OF
BCL2 GENE EXPRESSIONS
Raman Rajeshkumar1* and Dhanabal S.P2

1
Department of Pharmaceutical Biotechnology, Jagadguru Sri Shivarathreeshwara University,
Mysore, JSS College of Pharmacy, Ooty
2
Department of Pharmacognosy and Phytopharmacy, Jagadguru Sri Shivarathreeshwara
University, Mysore, JSS College of Pharmacy, Ooty
Abstract
Consumers are more concern towards the healthy diet to improve physical and mental well-being
status. In this context, plant bioactive compounds exhibiting functional activity could be played a
remarkable role to prevent chronic health disease, addition to the normal nutritive function.
Biologically moderate levels of ROS may function as signals to promote cell proliferation and
survival, whereas severe increase of ROS can induce cell death. This strategy was implemented
for cervical cancer cell death. In our study we created oxidative stress inside the cervical cancer
cell using two bioactive compounds (RD1 and RD2) isolated from Ethyl acetate and 50%
methanolic extracts of Symplocos cochinchinensis (lour.) Moore. Both compounds was found to
be an antioxidant in higher concentration and prooxidant in lower concentration. We carried out
induction of apoptosis and apoptotic DNA fragmentation, changes in mitochondrial membrane
potential, and profiles of gene and protein expression in response to treatment of cervical cancer
cell (HeLa). RD2 exhibited potential apoptotic effects on HeLa cells, and induced the apoptosis
through the events of up regulation of caspase 3, down regulation of bcl-2 genes followed by
cytochrome c release. By through this study we demonstrated efficacy of the RD2 as bioactive
compound in activating apoptotic pathway in HeLa cell line via oxidative stress induced down
regulation of BCl2 gene expressions. These findings provide a scope for future studies on RD2
as functional food for the treatment of cervical cancers.
Key Words: Functional food, antioxidant, prooxidant, Symplocos cochinchinensis.
81

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International Seminar & Exhibition on Phytopharmaceuticals Emerging Challenges and Opportunities, JSSCP, Ooty

Jagadguru Sri Shivarathreeshwara University

International Seminar & Exhibition on

TIFAC CORE in Herbal Drugs

JSS COLLEGE OF PHARMACY

ANTHELMINTIC AND ANTI OXIDANT ACTIVITY OF KARAMARDA FRUITS

PREPARATION AND EVALUATION OF GINGEROL PHYTOSOME LOADED

Singh Rudra Pratap*, Gangadharappa H. V., Mruthunjaya K.

JSS College of Pharmacy, Jagadguru Sri Shivarathreeswara University, Mysuru-570015, India.

ANTI-PYRETIC ACTIVITY OF AEWA HERBAL FORMULATION IN BREWER’S

HYPOGLYCEMIC EFFECT OF TRADITIONAL MEDICINE FORMULATION

(SNG-01) ON ALLOXAN INDUCED DIABETIC MICE

GC-MS ANALYSIS OF AQUEOUS EXTRACT AND ANNONA MURICATA MEDIATED

STABILITY OF PHYTOCHEMICALS AS SOURCES OF FREE RADICLE

Veena.B*, Anusuya devi.R and K.Thenmozhi

Department of Botany, Kongunadu Arts and Science College, Coimbatore-641029

PROXIMATE COMPOSITION, NUTRITIVE CONTENT AND PHYTOCHEMICAL

Anusuya devi.R *, Veena.B and K.Thenmozhi

Ph.D., Research scholar, Department of Botany,

Kongunadu Arts and Science College,

Key Words: Edible, Malasars, Irulars, nutritional, potential, phytochemicals.

NETWORK PHARMACOLOGY BASED APPROACH TO UNDERSTAND THE

R Logesh 1*, M. Suresh Kumar 1, S.P. Dhanabal 1 and S Rajan 2.

1. Department of Pharmacognosy and Phytopharmacy, J.S.S, College of Pharmacy, Ooty-643001,

Keywords: Lagenaria siceraria; Obesity; Lipase assay; drug-likeness prediction; systems

MODULATION OF SUPER OXIDE DISMUTASE ACTIVITY IN ARTHRITIC RATS

Sangeetha M1*, Chamundeeswari D1, Saravanababu C2

Rheumatoid arthritis (RA) is a chronic, progressive, systemic inflammatory disorder affecting

Key words: Ethanolic Leaf extract (ETLE), SOD-Super oxide dismutase

HEPATOPROTECTIVE EFFECT OF Mussaenda frondosa Linn. IN ALBINO RATS

Shanthi S*, Latha S, Chamundeeswari.D

Department of Pharmacognosy, Faculty of Pharmacy, Sri Ramachandra University, Porur,

Key words: Mussaenda, Extract, Heaptoprotective, Jaundice.

EFFECT ON ANTICANCER ACTIVITY OF EXTRACT OF NAREGAMI ALATA

WIGHT AND ARN AGAINST DAL AND EAC CELL LINES

P.Akila*, A.Abirami, Mohamed Halith and M.Nagarajan

Department of Pharmaceutics, K.M. College of Pharmacy, Madurai.

Key words: Naregamia alata, phytochemical screening, anti-cancer.

PHARMACOGNOSTICAL AND PRELIMINARY PHYTOCHEMICAL SCREENING

P.Divya*,M. Suganya, K. Murugeswari, V.Balu, M.Packia Lakshmi

K.M.college of Pharmacy, Madurai

IN- VITRO WOUND HEALING ACTIVITY OF DIPTERACANTHUS PATULUS LEAF

Esther.M*, Ganesh.H, Dr.Livingston Raja N.R.

KM College Of Pharmacy, Madurai.

Key words: Dipteracanthus patulus, chorioallantoic membrane model.

FORMULATION AND EVALUATION OF LORNOXICAM BY USING NATURAL

J.Priyadharshini , M.ISMATH FARHA*, S. Mohamed Halith, M.Nagarajan

Department of Pharmaceutics, K.M. College of Pharmacy, Madurai, Tamil Nadu.

G. Mukesh*, A.Senthamil Selvi, M. Sundara Pandian, M.Nagarajan

RECENT TRENDS OF PLANT CELL ELICITATION IN PRODUCTION OF

A. Noorul Asif*, A.G.HariHaran, S. Mohammed Halith and M. Nagarajan

Department of Pharmaceutics, K. M. College of Pharmacy, Madurai

FORMULATION AND CHARACTERIZATION OF HERBAL LIPSTICK

L.S. Sathyapriya, V.Sathya Praba*, A.G.Hariharan, S. Mohamed Halith and M.Nagarajan

Department of Pharmaceutics, K.M. College of Pharmacy, Madurai, Tamil Nadu.

EFFECT OF FIBRINOLYTIC ACTIVITY ON HIBISCUS ROSA SINENSIS

A.Aruna, S.MohamedHalith, M.Nagarajan, S.Soniya*

Department of pharmaceutics, K.M College of pharmacy, Madurai.

Keywords: Fibrinolytic activity, Hibiscus rosasinensis, aqueous extract.

PREPARATION AND STANDARDISATION OF HERBAL JUICE (FICUS CARICA

Key Words: Figs, Nutraceuticals, Evaluation, Juice

EFFETCT OF VITEXIN ON HYPOXIC STRESS IN SOLID TUMORS: AN INSIGHT

Sandhya Chipurupalli1*, Elango Kannan1

Keywords: Vitexin, Endoplasmic reticulum stress, hypoxia, hypoxia-inducible factor 1,