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gallus IMMUNOTECH INC .

producer of egg-derived antibodies


PRODUCT SPECIFICATIONS
Product: Aff. Pur. Chicken anti-human osteopontin-c (the shortest splice variant of
osteopontin)
Product No.: AhOPNc
Lot Number:
Physical State: liquid
Antibody Concentration:
Volume:
Storage Diluent: Phosphate buffered saline, pH 7.2
Preservative: 0.05% Sodium Azide
Procedure: A chicken was immunized with a peptide representing the splice junction of
osteopontin-c. After multiple immunizations, eggs were collected and IgY was purified from the
egg yolk. The antibody was affinity purified on an osteopontin-c peptide column.
Specifications: For Western blotting, recommended concentration 1 ug/ml, suitable for
immunohistochemistry.
vector

OPN-c
OPN-a
OPN-c
OPN-a
OPN-a
OPN-c

66kD

hOPN-c hOPN-c O-17


Left panel: supernatants from transfected MCF-7 breast
tumor cells probed with the anti-hOPNc antibody (a prior
Western blot of the same supernatants had demonstrated
the comparable expression levels of osteopontin-a and -c).
Right panel: 400 ng of GST-OPN was loaded per lane,
probed with anti h-OPNc, stripped, and reprobed with anti-
pan-osteopontin O17.

R.R.#3, Fergus, Ontario, CANADA N1M 2W4 Phone (Toll-free):(877) 4-GALLUS


www.chickenantibodies.com Fax: (519) 843-2928
E-mail: info@gallusimmunotech.com