SEEING IS

KNOWING
OneSight
next-generation cfDNA analysis
OneSight is a novel analysis research platform that enables genetic labs to assess the aneuploidy status
of chromosomes and chromosome segments in NGS data from cell-free DNA samples. OneSight enables
geneticists to define classification rules to prioritize aberrant chromosomes for detailed visual inspection.
OneSight’s genome-wide visual representation provides unique insights in the underlying data.

Visualize and assess all chromosomes

Today’s commonly used cell-free chromosomal analysis pipelines typically assess the aneuploidy status of only a few selected
chromosomes. In reality, aneuploidies can occur in all chromosomes – and moreover, may affect only a part of the chromosome.
OneSight enables genome-wide visualization of copy number proiles in cell-free DNA, covering full chromosomes as well as
chromosome segments.

Figure 1: OneSight‘s visual plots provide unique insights in the data. Depending on the lab-deined classiication rules, chromosomes are automatically labeled
thereby prioritizing them for further investigation if needed.

Flexible and automated classification

Most chromosomal analysis pipelines are limited in the way they can classify chromosomes. OneSight allows geneticists to deine
lexible, user-friendly decision rules in which a wide set of different parameters and threshold values can be combined to enable the
automated classiication of chromosomes. The research platform also enables you to boost analytical strength through selection of
reference samples as a user-deined reference set.

Improved noise reduction

Many of today’s commonly used chromosomal analysis pipelines are impacted by confounding factors such as recurrent technical
variation, segmental chromosomal variation across individuals or genome-wide chromosomal instability (as is the case for many cancer
 Figure 2: With OneSight‘s intuitive rule-builder, lab’s can deine their own decision rule set to enable automated
classiication of chromosomes.

types). OneSight excludes regions that are likely to cause recurrent variation across experiments, thereby minimizing the impact of
recurrent variations on the chromosomal analysis of cell-free DNA, allowing geneticists to focus on relevant segmental chromosomal
variation and genome-wide chromosomal instability. In addition, OneSight offers a unique Quality Score (QS) that allows you to quickly
assess the quality of the sample.

A cost-effective turnkey solution compatible with the most common NGS platforms

With OneSight, NGS analysis of chromosomal aneuploidies in cell-free DNA no longer requires large upfront investments and
new infrastructure. It’s a cost-effective turnkey solution through the proven Software as a Service model, making it possible for labs
to easily perform cfDNA analysis research. OneSight is compatible with the most common high-capacity Next-Generation Sequencing
platforms and library prep kits. By combining standard NGS reagents and worklow with the OneSight platform, assessing the aneuploidy
status of chromosomes and chromosome segments in cell-free DNA is now possible with minimal impact on you IT infrastructure.

OneSight

Compatible with all Compatible with the most Upload raw Select analysis pipeline Visually inspect
NGS library prep kits common NGS platforms sequencing data and reference set chromosome plots

Figure 3: OneSight is a cost effective turnkey solution for cfDNA analysis research. The platform is compatible with the most common NGS platforms and
library prep kits, and makes cfDNA analysis research possible with minimal impact on your IT infrastructure.
 Insight through visualization
• Understand your sample using
OneSight’s unique visual representation
and set of statistical parameters
• Look at all chromosomes in a single
analysis
• Easily assess the quality of the sample
using OneSight’s unique Quality Score,
and minimize the impact of recurrent
technical variation using a lab-deined
reference set
• Assess the presence of biological
confounding factors (e.g. segmental
aberrations, genome-wide chromosomal
instability)

A cost-effective, scalable solution

• Leverage your available NGS capabilities
and resources with minimal impact on
your IT team
• Increase your eficiency using OneSight’s
automated classiication
• Work with a knowledgeable team that
understands your lab’s requirements
• Scalable from low to high volumes.
• Avoid large licensing and setup costs

A robust technology, supported by

an experienced team
• Validated internally and by early accesss
Figure 4: An extract from OneSight’s extensive report. Based on the decision rules set, the chromosomes users on a large number of samples and
selected for analysis are labeled, prioritizing them for further investigation if needed. supported by a body of literature on the
underlying technology.
• Developed by Cartagenia, now part of
Agilent Technologies, leveraging the
company’s unique expertise of software
solutions for genetics labs.

For Research Use Only. Not for use in diagnostic procedures.

LEARN MORE TODAY:

www.cartagenia.com

PR7000-0151
© Cartagenia 2016
Printed in USA, May 1, 2016
5991-6674EN