Grace’s Insect Medium,

Unsupplemented and serum-free; ideal for

transfection.

Description
G race’s Insect Medium was developed in 1962 and has Box 1 | Basic Info
been shown, with the addition of serum, to support
the growth of insect cells, including Spodoptera frugiperda. Cat. No. ABP-MED-10004
Allele’s Grace’s Insect. Culture Medium (Unsupplemented) Contents
is serum-free and ideal for transfection because it does
1 liter of 1X sterile liquid
not contain lactalbumin hydrolysate, or yeastolate which
can interfere with transfection. When supplemented with
L-glutamine, lactalbumin hydrolysate, and yeastolate Storage
Grace’s Insect Medium can be used for general growth and Store at +2°C to +8°C, dark. Stable for 2 years if
stored properly.
maintenance of insect cells.
Usage Details
♦ Contains 50 µg/ml gentamycin.

♦ Optimum growth temperature is 27 ± 1°C

Box 2 | Specifications
♦ Optimum pH is 6.1-6.2
Test Method Specification
♦ Most insect cell lines can be transferred directly into pH Potentiometric 6.1-6.2
Grace’s Insect Culture Medium without adaptation. Seeding
densities should be 5 x 105 - 1 x 106 viable cells per milliliter
of culture. Appearance Visual Clear Yellow
Inspection Solution
♦ Sterile serum should not be re-filtered before or after Sterility Membrane No Growth
being added to sterile medium because growth promoting filtration
capacity may be reduced upon re-filtration.

♦ Medium should be clear of particulates and flocculent

material. Do not use if liquid medium is cloudy or contains
precipitates. Other evidence of deterioration may include
color change or degradation of physical or performance
characteristics. Website: www.allelebiotech.com
Call: 1-800-991-RNAi/858-587-6645
(Pacific Time: 9:00AM~5:00PM)

F or Research Use Only. Not for Diagnostic or Email: oligo@allelebiotech.com

Therapeutic Use.
Purchase does not include or carry any right to resell or For Technical Support:
transfer this product either as a stand-alone product or as
Email:oligo@allelebiotech.com
a component of another product. Any use of this product
other than the permitted use without the express written
authorization of Allele Biotech is strictly prohibited

Related Products
 Sapphire™ Baculovirus DNA  Sapphire™ Insect Transfection Kit
 High level expression. The p10 promoter is  Ideal for easy and efficient transfection of DNAs
partially deactivated and the lytic p10 gene is deleted into insect cells.
so that transcription levels are higher due to reduced
interference and healthier insect cells.
 Serum Free Insect Culture Medium
 Sf9,Sf21, and T.ni Insect Cell Lines  Specifically developed for the Baculovirus
 Commonly used to isolate and propagate Expression Vector System (BEVS) technology.
recombinant baculoviral stocks and to produce
recombinant proteins.

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