Bioassay Bahan Alam

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 Gambaran Umum

Antimikroba Senyawa Membasmi

MIKROBA Merugikan makhluk hidup

Klasifikasi :

1. Berdasarkan struktur kimia

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 2. Berdasarkan aksi
utamanya

a. Bakteroostatik

b. Bakterisida

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 3. Berdasarkan
spektrum aktivitasnya

a. Spektrum luas

b. Spektrum sempit

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 4. Berdasarkan tempat
kerjanya

1. Dinding sel

2. Membran sel

3. Asam Nukleat

4. Ribosom
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In vitro Antimicrobial Assay and Phytochemical
Analysis of Ethanolic Extracts of Voacanga
africana Seeds

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 Biji Voakanga (Voacanga needs)

Ekstraksi

Ethanol

Hot extraction Cold extraction

(Soxhlet) (percolation)

Extract Ethanol
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 Antimicrobial Assay

Medium Na Medium SDA

- E. coli,
- Pseudomonas aeruginosa
- Serratia marcescen
- Staphylococcus aureus
- Candida albicans
- Aspergillus flavus,
Bakteri Uji Jamur - A. niger,
- Alternaria solani
- Rhizopus stolonifer

Kontrol :
- gentamisin
- clotrimazole
Ekstrak Et-OH
Voacanga africana needs Inkubasi 1 x 24 jam Inkubasi 15 hari

Konsentrasi :
25 g/ml, 50 g/ml,
100g/ml, 150g/ml,
200g/ml, 250g/ml, Suhu 370C
dan 500g/ml

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 Setelah Inkubasi

Ukur Zona
Hambatannya

Bakteri Jamur

+ + + + + + + + +
Escherichia Staphylococcus Pseudomonas Serretia Candida Rhizopus Aspergilus Alternaria
Albicans A.niger
coli aureus aeruginosa, marcescen stolonifer flavus solani

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Table 1: Phytochemical Analysis of the Seeds Extracts of Voacanga africana

Ket :
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(+) = Positif, (-) = Negatif
Table 2: Sensitivity Test for The Bacterial and Fungal species on the
seeds extracts of Voacanga africana

Key:
HEE ------Hot Ethanol Extract CEE ------Cold Ethanol Extract
EH ------ Empty Hole GH ------ Gentamicin Hole
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CH -------Clotrimazole Hole
Table 3: Minimum Inhibitory Concentration (MIC) of The Seeds Extracts
of Voacanga africana

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 Duru, Christopher M. and Nkechi, E.Onyedineke. 2010. In vitro
Antimicrobial Assay and Phytochemical Analysis of Ethanolic Extracts of
Voacanga africana Seeds. Journal of American Science

Ganiswara, 2007. Farmakologi dan Terapi Edisi V, Fakultas Kedokteran

Universitas Indonesia, Jakarta

Mycek, 2001. Farmakologi Ulasan Bergambar, Widya Media, Jakarta

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