1.

Prevalence, antimicrobial susceptibility and associated risk factors

of shigella and salmonella species among diarrheic pediatric
populations attending at Gondar town health institution, Northwest
Ethiopia.
Objectives

2.1. General objectives

 To determine the prevalence, antimicrobial susceptibility and associated

risk factors of shigella and salmonella species among diarrheic pediatric
children attending at Gondar town health institutions.
2.2. specific objectives
 To determine the prevalence of shigella and salmonella species among
diarrheic pediatric children
 To determine the antimicrobial susceptibility patterns of shigella and
salmonella species among diarrheic pediatric children.
 To assess the associated factors for shigellosis and salmonellosis in
diarrheic pediatric populations attending at Gondar town health
institutions.
2.3. Methodology
Across-sectional study will be conducted in Gondar town health institutions among
diarrheic pediatric children. The mother or guardian will be given a clean plastic
stool container and oriented about sample collection after interviewed with
pretested questionnaires. Once collected, protozoa parasites will be identified
through direct microscopy using saline wet mount at each study sites and part of
the stool will be kept in Cary-Blair transport media, and transported using ice box
to microbiology department of Gondar university for further microbiological
investigations. Inoculation of the specimens on the standard culture media. The
primary isolates will be subculture on the available biochemical tests for further
identification. Antimicrobial susceptibility tests will be carried out by using
disc diffusion method using Mueller-Hinton agar. A standardized suspension
of the bacterial isolate will be prepared and turbidity of the inoculum will be
compared with 0.5 McFarland turbidity standard.