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Chapter 7
Photosynthesis
Heterotrophs (consumers/
decomposers)
-obtains organic food by eating other organisms or their
by-products
Oxidation: partial or
complete loss of electrons
-exergonic (release
energy)
Reduction: partial or
complete gain of
electrons -endergonic
(absorb energy)
A. Photosynthetic Reaction
1. In 1930 C. B. van Niel showed that O2 given off by
photosynthesis comes from water and not from CO2.
2. The net equation reads:
light
6H2O + 6CO2 C6H12O6 + 6O2
Carbon
Water + dioxide Glucose + oxygen
in the chloroplast
How
are
they
connected?
Heterotrophs
making energy & organic molecules from ingesting organic molecules
Organic molecules
(carbs!) built by
photosynthesis provide
both the building blocks
and energy for cells.
Figure 7.1b
- Stoma: opening in
the leaf to exchange
gas
aka: stomata
Figure 7.1c
Chlorophylls
and other
pigments
involved in
absorption of
solar energy
reside within
thylakoid
membranes
of
chloroplasts
4.
The
chloroplast
§ Stomata:
opening
in
leaf
for
gas
exchange
§ Pigment:
chlorophyll
§ Mesophyll:
Plant
cell
photosynthe>c
layer:
Sites
of
photosynthesis
(Double
membrane)
granum
(stack)
Thylakoids
(pancake)
contains:
• chlorophyll
molecules
• electron
transport
chain
• ATP
synthase
-‐
Loca>on
of
light
reac>on
Stroma-‐fluid-‐filled
interior
(syrup)
-‐
Loca>on
of
dark
reac>on
Quick Check - FIVE OR FEWER
1. Chloroplast
2. Thylakoid
Stroma: liquid in
chloroplast
4. Organic Molecules involved
Stoma: pore in
leaves
7.2 Plants as Solar Energy Converters
1) As different
wavelengths are
passed through, some
are absorbed.
2) Graph of percent of
light absorbed at each
wavelength is
absorption spectrum
.
Absorption spectrum
3) Photosynthesis produces
oxygen; production of oxygen is
used to measure the rate of
photosynthesis.
4) Oxygen production and,
therefore, photosynthetic activity
is measured for plants under
each specific wavelength;
plotted on a graph, this action spectrum
produces an action spectrum.
5) Since the action spectrum
resembles absorption spectrum,
this indicates that chlorophylls
contribute to photosynthesis.
Checkpoint:
http://www.glencoe.com/sites/common_assets/science/virtual_labs/
LS12/LS12.html
1. What is the relationship between the absorption spectrum and the
action spectrum?
in the chloroplast
Inside a Chloroplast
H 2O CO2
Light
NADP+
ADP + P
Light- Calvin
Calvin Cycle
dependent
Chloroplast
Hall
O2 Sugars
C6H12O6
1. Light reactions cannot take place unless light is present.
They are the energy-capturing reactions.
b. Chlorophyll within thylakoid membranes absorbs solar
energy and energizes electrons.
c. Energized electrons move down the electron transport
system; energy is captured and used for ATP production.
d. Energized electrons are also taken up by NADP+, becoming
NADPH.
2. Calvin Cycle
Reactions
a. These reactions take
place in the stroma; can
occur in either the light
or the dark.
b. These are synthesis
reactions that use
NADPH and ATP to
reduce CO2.
-- and make a
carbohydrate, sugar
What you should know by now..
1. The equation for photosynthesis. Write it!
Inside
thyloakoid
Thylakoid
membrane
Stroma
Copyright Pearson Prentice Hall
Photosystems:
Light
harves>ng
units
of
the
thylakoid
membrane
Photosystem II
Photosystem II
Electron
High-energy
carriers
Copyright Pearson Prentice Hall
electron
Light-Dependent Reactions
2. Enzymes on the thylakoid membrane break water
molecules into:
Photosystem II
2H2O
Electron
High-energy
carriers
Copyright Pearson Prentice Hall
electron
Light-Dependent Reactions
-hydrogen ions
-oxygen atoms
-energized electrons
Photosystem II
+ O2
2H2O
Electron
High-energy
carriers
Copyright Pearson Prentice Hall
electron
Light-Dependent Reactions
Photosystem II
+ O2
2H2O
High-energy
Copyright Pearson Prentice Hall
electron
Light-Dependent Reactions
Photosystem II
+ O2
2H2O
High-energy
Copyright Pearson Prentice Hall
electron
Light-Dependent Reactions
Photosystem II
+ O2
2H2O
Pc
Pq
Cytochrome
High-energy complex
Copyright Pearson Prentice Hall
electron
Light-Dependent Reactions
space.
Photosystem II
+ O2
2H2O
Pc
Pq
Cytochrome complex:
catalyzing the transfer of
Cytochrome electrons from
complex
Copyright Pearson Prentice Hall plastoquinol to
plastocyanin
Light-Dependent Reactions
Photosystem II
+ O2
2H2O
Pc
Pq Cytochrome
complex
Photosystem I
Copyright Pearson Prentice Hall
Light-Dependent Reactions
+ O2
2H2O
Cytochrome
complex
Photosystem I
Copyright Pearson Prentice Hall
Light-Dependent Reactions
+ O2
2H2O
2 NADP+
2
2 NADPH
Copyright Pearson Prentice Hall
Light-Dependent Reactions
+ O2
2H2O
2 NADP+
2
2 NADPH
Copyright Pearson Prentice Hall
Light-Dependent Reactions
+ O2
2H2O
2 NADP+
2
2 NADPH
Copyright Pearson Prentice Hall
Light-Dependent Reactions
+ O2
2H2O
2 NADP+
2
2 NADPH
Copyright Pearson Prentice Hall
Light-Dependent Reactions
ATP synthase
+ O2
2H2O
2 NADP+
2
2 NADPH
Copyright Pearson Prentice Hall
Light-Dependent Reactions
+ O2
2H2O
2 NADP+
2
2 NADPH
Copyright Pearson Prentice Hall
Light-Dependent Reactions
ATP synthase
+ O2
2H2O
2 NADP+
2
2 NADPH
Copyright Pearson Prentice Hall
Light-Dependent Reactions
ATP synthase
+ O2
2H2O
ADP
2 NADP+
2
2 NADPH
Copyright Pearson Prentice Hall
Light-Dependent Reactions
+ O2
2H2O
ADP
2 NADP+
2
2 NADPH
Copyright Pearson Prentice Hall
Photosystems
of
photosynthesis
antenna
pigments
1. Light hits photosystem II and excites an electron, H20
2. The primary electron acceptor passes the electron down the ETC and
generates ATP
3. Light is required for PSI, but not water, it generates NADPH
Figure 7.5
Indicate which system (PS1 or PS2 or BOTH)
____1.
PS2 Splits water
____2.
PS1 Produces NADPH
____3.
Both Has an electron transport chain
Both
____4. Requires light
Both
____5. Utilizes a primary electron acceptor
Both
____6. Occurs in the thylakoid
____7. Requires the input of H20
PS2
Both
____8. The cyclic path
Both
____9. Uses chlorophyll
PS2
____10. Releases oxygen
____11. chlorophyll a
PS2
PS1
____12. chlorophyll b
ETC of Photosynthesis
chlorophyll a
Photosystem II
chlorophyll b
Photosystem I
ETC of Photosynthesis
sun sun
H+ H+ H+
O H+ H+ H+
H+ + + H+ to Calvin Cycle
H+ H H
split H2O
ATP
ETC of Photosynthesis
sun sun
H+ H+ H+
O H+ H+ H+
H+ + + H+ to Calvin Cycle
H+ H H
split H2O
ATP
Two Pathways of Light Reactions
1. Noncyclic
2. Cyclic
ATP
Cyclic
photophosphoryla>on
• If
PS
I
can’t
pass
electron
to
NADP…it
cycles
back
to
PS
II
&
makes
more
ATP,
but
no
NADPH
✗
– coordinates
light
reac>ons
to
Calvin
cycle
– Calvin
cycle
uses
more
ATP
than
NADPH
ATP
18 ATP +
12 NADPH → 1 C6H12O6
Photophosphoryla>on
cyclic
photophosphorylation
NADP
NONcyclic
photophosphorylation
ATP
Are you still confused? This is pretty
hard to visualize, but through the magic
of technology, we can watch these
processes as animations
McGraw Hill Animation:
http://highered.mheducation.com/sites/0072437316/
student_view0/chapter10/animations.html#
https://www.youtube.com/watch?v=g78utcLQrJ4
Figure 7.7
https://www.youtube.com/v/mYbMPwmwx88
Chemiosmosis is difficult to visualize.
- Stoma:
opening in
the leave to
exchange
gas
The
Calvin
Cycle
1950s | 1961
Whoops! Wrong Calvin…
The Calvin Cycle
Also called
Summary Statements:
Where does the cell get its energy to perform these reactions?
C C C C C
1C CO2
C C C C C 1. Carbon fixation
3. Regeneration
of RuBP C C C C C C C C C C C
RuBP 5C RuBisCo C C C C C C
ribulose bisphosphate
starch,
sucrose, 3 ATP ribulose
6C C C C C C C
cellulose bisphosphate
& more 3 ADP carboxylase
used
5C C C C
C C C
to make glyceraldehyde-3-P C C C
glucose PGA 3C C C C
G3P phosphoglycerate
C C C C C C C C C
C= C= C C C C 3C C C C
C C C
C C C 2. Reduction 6 ATP
H H H
| | | C C C 6 NADPH 6 ADP
|
H
C– C– C
|
H
|
H C C C 3C
6 NADP
Molecules of Calvin Cycle
Light
reac>ons
produced
ATP
produced
NADPH
consumed
H2O
produced
O2
as
byproduct
Calvin
cycle
consumed
CO2
produced
G3P
(sugar)
regenerated
ADP
regenerated
NADP
ADP NADP
Types of phosphorylation
Alternative Pathways
C3 PLANTS: use the normal Calvin Cycle exclusively to fix
carbon, the MOST Common Pathway
C4 plants and
CAM plants use
an alternate
pathway to FIX
carbon dioxide
from the air.
Reducing
photorespira>on
NADP
NONcyclic
photophosphorylation
ATP
First, finish the ETC coloring and questions
Then:
QOD:
Ch
7
“Test
Yourself”
• Answer
mul>ple
choice
ques>ons
“1-‐22
on
pg131-‐132
• Finish
for
HW
(check
answers
in
back
of
book)
Leave space for QOD: Ch 7 “Test Yourself” if needed
QOD: photosynthesis overview
copy and fill out this table as best you can.
Process Light? Location Reactant Product
(photosynt
hesis)
1. ETC
2. Calvin
Cycle
- Stoma:
opening in
the leave to
exchange
gas
Quick Practice
grana
thylakoid
stroma
O2
Summarize what is happening at 1, 2 ,and 3
C C
C
C C C C C
1C CO2
C C C C C 1. Carbon fixation
3. Regeneration
of RuBP C C C C C C C C C C C
RuBP 5C RuBisCo C C C C C C
ribulose bisphosphate
starch,
sucrose, 3 ATP ribulose
6C C C C C C C
cellulose bisphosphate
& more 3 ADP carboxylase
used
5C C C C
C C C
to make glyceraldehyde-3-P C C C
glucose PGA 3C C C C
G3P phosphoglycerate
C C C C C C C C C
C= C= C C C C 3C C C C
C C C
C C C 2. Reduction 6 ATP
H H H
| | | C C C 6 NADPH 6 ADP
|
H
C– C– C
|
H
|
H C C C 3C
6 NADP
What Factors the Affect Photosynthesis?
ADP
NADP
ATP
NADPH
O2 glucose
A = photosystem II AB = ATP
B = photosystem I AC = phospholipids
C = H20 AD = light (energy)
D = Electron Transport Chain
E = ATP Synthase
Photosynthesis Activities
Answer:
The light reactions of photosynthesis occur along the
thylakoid membrane. The Calvin cycle occurs in the
stroma, surrounding the thylakoids.
Standardized Test Prep
Chapter 6
Extended Response
Answer:
Part A The reactants for the light reactions of
photosynthesis are sunlight, water, NADP+, and
ADP. The products are oxygen, ATP, and NADPH.
The reactants for the Calvin cycle are ATP, NADPH,
CO2, and RuBP. The products are NADP+, ADP, and
organic compounds.
Part B ADP/ATP, NADP+/NADPH, and electrons are
recycled during photosynthesis. RuBP, which reacts
with CO2 in the Calvin cycle, is regenerated at each
turn of the cycle.
Review of ETC of Photosynthesis
sun
e
e
Photosystem II
P680
chlorophyll a
Inhale, baby!
ETC of Photosynthesis
chloroplast thylakoid
+H+ H+ H+
H+ H+H
H+H H H H
+ + + +
+H+ H+ H+
H+ H+H
H+H H H H
+ + + +
ATP
+H
e- H+
e-
e e
Photosystem II
P680
chlorophyll a
ETC of Photosynthesis
chloroplast thylakoid
H +H+ H+ H+
H H + + H+H+ H+
+ +
H H
+H+ H+ H+
H+ H+H
H+H H H H
+ + + +
ATP
3
1
2
e H+
e
4 ATP
H+
H+
to Calvin Cycle
H+ H+ H+
H H
+
H+ + H+
energy to build
carbohydrates
Photosystem II
P680 ADP + Pi
chlorophyll a ATP
H+
ETC of Photosynthesis
e
e
sun
e e
Photosystem I
P700
Photosystem II
chlorophyll b
P680
chlorophyll a
ETC of Photosynthesis
electron carrier
e
e
5
sun
Photosystem I
P700
Photosystem II
chlorophyll b
P680 $$ in the bank…
chlorophyll a reducing power!