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Biotechniques CA1

1. Explain distribution coefficient and its role in chromatography.


2. Describe the concept of theoretical plates in chromatography.
3. What are the components of a basic column chromatographic system?
4. Differentiate between following terms: Normal vs reverse phase chromatography; isocratic vs
gradient elution.
5. Elucidate the principle of HPLC. Why degassing of mobile phase is required for HPLC?
6. Which factors are responsible for peak broadening in chromatography?

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