1. Explain distribution coefficient and its role in chromatography.
2. Describe the concept of theoretical plates in chromatography. 3. What are the components of a basic column chromatographic system? 4. Differentiate between following terms: Normal vs reverse phase chromatography; isocratic vs gradient elution. 5. Elucidate the principle of HPLC. Why degassing of mobile phase is required for HPLC? 6. Which factors are responsible for peak broadening in chromatography?