The Science of Legume: Black Beans In the Prevalence of Multiple

Cancers' Risk

Kyle J. Norton

People who turn to alternative medicine for treatment of disease in avoidance

of adverse effects induced by conventional medicine should be patient. In
compared to herbal medicine, food therapy takes significant longer in times to
ease symptoms, depending to stages of the treatment which directly address to
the cause of disease.

Epidemiological studies strongly suggested that intake of black bean may

associate to reduced risk and treatment of various type of cancers(1)(2).

Cancer is a class of chronic condition characterized by the irregular cell growth

in the body organs. Sometime, the cancer cells can spread to other tissues or
organs depending to stages of cancer..

Black bean, a small roughly ovoid legume with glossy black shells, genus
Phaseolus, belongings to the family Fabaceae can be bought in most grocery
stores all around the year in dried and canned forms. It is believed that black
bean was first domesticated growth in South America.

1. Liver Cancer
Liver cancer is a condition of the abnormal growth of the cells in the liver's
tissue. According to statistics approximated 40,710 adults (29,200 men and
11,510 women) in the United States will be diagnosed with primary liver
cancer this year.

According to the Stocking Hall, Cornell University, phytochemicals isolated

from black bean such as triterpenoids, flavonoids, and other compounds
exhibited antiproliferative activities against the HepG2 cells(3), the human liver
cancer cell line.

The efficacy of black bean in ameliorated growth of liver cancer may be

attributed to the presence of some potent antioxidants in expression of
apoptosis.

Consumption of bean not only played a prominent role of gene expression(4)

involved the regulation of gene Hspa8 in reduced risk of liver disease
developed, but also addressed the important issue of attenuated hepatic stress or
toxicity in rat model.

Increased bean consumption in rats exhibits changes indicative of hepatic

damage, Dr. Daniell EL, the lead author said, "Dry bean (Phaseolus vulgaris L.)
consumption is associated with reduced risk for a number of chronic diseases",
such as liver cancer.

Dietary bean concentrations.is found to associate to the cholesterol 7α

hydoxylase and UDP-glucuronosyltransferase genes(4) increased with
connection to the development of liver cancer, in a dose-dependent manner.

2. Breast Cancer
Breast cancer in most cases, starts in the tissues of the breast either from the
inner lining of milk ducts (Ductal carcinoma) or the lobules (Lobular
carcinoma) that supply the ducts with milk. but in rare instance, breast cancer
may arise from other areas of the breast.

In the study of water-soluble condensed tannins isolated from black beans

against Hs578T breast cancer cells, the preparation at 0.24-24 microM in dose-
dependent induced cancer cell death by apoptosis but did not affect the growth
of normal cells(5), as shown by observation assay.

The efficacy of water-soluble black bean condensed tannins at 24 microM in

suppressed the breast caner cells(5) may be attributed to the phytochemical in
reduced coagulation of remained blood in stimulated cancer cell migration and
regulated the growth of breast tumor in formation of new blood vessels.

According to the National Taiwan University, fermentation product of soybean,

black bean, and green bean mixture (BN999) showed a significant effect in
inhibited the growth of human breast cancer AU565 cells in increased
expression of multiple apoptotic regulators in induced apoptosis(6).

The fermentation product also exerted an impressive activity in induced

apoptosis in various human cancer cells but not in normal human cells, Dr,
Chia JS, the lead author insisted.

Dr. Dong M, the lead researcher at the Cornell University, in the finding of
major phytochemicals of black bean which may have a powerful effect in
treatment of breast cancer suggested, that out of newly found flavonoids, only 6
(5, 7, 8, 9, 11, 19, 20) of them showed potent antioxidant and antiproliferative
activities.against MCF-7 human breast cancer cells(7).

3. Osteocarcinoma
Osteocarcinoma is a condition of bone cancer caused by irregular cell growth in
bone, affecting mostly adolescents and young adults..

According to the The Chinese University of Hong Kong, hemagglutinin

isolated from an edible legume, namely black bean, showed an anti
proliferative activity(8) against tumor cell lines MG-63(8) and HepG2(8) but
not the normal cell line.

Malignant osteocarcinoma cell treated with the phytochemical also exerted a

significant effect in shrinking of tumor cells as well as inducing apoptosis.

The study also stated that hemagglutinin also induced cell death through
interfering the first stage of tumor cell division in expression of protein, RNA,
and DNA and inducing mitochondrial membrane depolarization(8) in
controlling plasma membrane of a muscle or nerve cell

Furthermore, the study also suggested that the efficacy of hemagglutinin in

causing cell death may be attributed to the function of protein in increased
regulation of pro inflammatory expressions(8)
.
Dr. Dan X, the lead author said, " a novel hemagglutinin from an edible legume
(showed) a growth-inhibitory effect on osteocarcinoma and liver cancer cells"
and " The hemagglutinating activity of this hemagglutinin was demonstrated to
be ion dependent and stable over a wide range of temperature and pH values".

4. Prostate Cancer
According to the National Taiwan University,fermentation products BN999,
included black bean processed an anti prostate cancer effect through expression
of prostate adenocarcinoma PC-3 cell line.

BN999, the product mixture induced cancer cell death by activating the protein
gene involved pro cancer cell apoptosis via cell mediatory activities(9),
including a network of membranous anatomical structure and double
membrane-bound organelle in energy conversion.

Most importantly, BN999 target prostate and other cancer cell without affecting
the normal human cells and inducing obvious side effects(9).
In the investigation of water-soluble black bean condensed tannins isolated
from black beans showed a significant activity in induced cancer cell death by
apoptosis without affecting the growth of normal cells, in dose dependent
manner(10).

Additionally, the study also addressed that the efficacy of water-soluble in

induced prostate cancer cell death may be attributed to direct effect in decrease
cancer cell energy molecule and formation of cell structure.

Dr. Bawadi HA(10), the lead author indicated, after 24 h of injection, water-
soluble black bean condensed tannins showed a strong reduced coagulation of
blood in stimulated cancer cell migration and regulated mechanisms involved
growth of prostate cancer in formation of new blood vessels.

5. Colon Cancer
Colon cancer is a medical condition of the abnormal proliferation of cells in
large intestine.

In a study of rats injected with azoxymethane (AOM)-induced colon cancer or

saline and one week later administered an AIN, black bean (BB) and soy flour
(SF)--based diet, researchers found that mice fed with black bean showed a
significant expression in attenuated gene expression in coloncarcinogenesis by
modulating cellular kinetics and reducing inflammation(11).

Others experiment to test the prevalence of high bean intake and low incidence
of colon cancer in many Latin American countries in 53-wk-old weanling male
F344 rats randomly assigned by weight to the following groups: control (11
rats), casein diet (21 rats), and bean diet (21 rats), with animals fed the casein
and bean diets treated with the carcinogen azoxymethane (AOM) once weekly
for 2 wk, the results returned showed a significantly fewer colon
adenocarcinomas (P < 0.05) in rat fed with bean diet in compared control and
rats fed the casein diet(12).

Rats fed with bean diet also expressed a lesser in tumor multiplication observed
by tumors per tumor-bearing rat than in casein-fed rats.

Dt. Hughes JS, the lead researcher said, "dry beans contain anticarcinogenic
compounds capable of inhibiting AOM-induced colon cancer in rats. However,
the specific anticarcinogenic components within dry beans have not been
identified".
But suggested that the efficacy of bean in reduced colon cancer risk may be
results of the dietary fiber, phytochemicals or other components within dry
beans(12),...

Promisingly, the results of rats fed a modified AIN-93G diet (control) or diets
containing 75% black beans or 75% navy beans for 4 wk, and then colon
cancer initiated by administration of two injections of azoxymethane 1 wk
apart, also postulated a lower incidence of colon cancer in rats fed the black
bean (9%) in compared navy bean (14%) diets than in rats fed the control diet
(36%) at 31 weeks(13).

Rats fed the black bean also significantly lower of total tumor multiplicity in
compared to control(13).

The findings suggested that black bean with abundant bioactive chemical
compounds such as genistein, flavonols and saponins.may be considered as
functional food and integrated form of injection for reduced risk and treatment
of various types of malignant tumors. However, raw black beans containing
phytohenagglutinin can be toxic to liver in high amount(14).

Sources
(1) Common Beans and Their Non-Digestible Fraction: Cancer Inhibitory
Activity—An Overview by Rocio Campos-Vega,1,* B Dave Oomah,2,†
Guadalupe Loarca-Piña,1,† and Haydé Azeneth Vergara-Castañeda3(PMC)
(2) Fermentation product of soybean, black bean, and green bean mixture
induces apoptosis in a wide variety of cancer cells by Chia JS1, Du JL, Wu MS,
Hsu WB, Chiang CP, Sun A, Lu JJ, Wang WB.(PubMed)
(3) Phytochemicals of black bean seed coats: isolation, structure elucidation,
and their antiproliferative and antioxidative activities by Dong M1, He X, Liu
RH.(PubMed)
(4) Dietary dry bean effects on hepatic expression of stress and toxicity-related
genes in rats by Daniell EL1, Ryan EP, Brick MA, Thompson HJ.(PubMed)
(5) Inhibition of Caco-2 colon, MCF-7 and Hs578T breast, and DU 145
prostatic cancer cell proliferation by water-soluble black bean condensed
tannins by Bawadi HA1, Bansode RR, Trappey A 2nd, Truax RE, Losso JN.
(PubMed)
(6) Fermentation product of soybean, black bean, and green bean mixture
induces apoptosis in a wide variety of cancer cells by Chia JS1, Du JL, Wu MS,
Hsu WB, Chiang CP, Sun A, Lu JJ, Wang WB.(PubMed)
(7) Phytochemicals of black bean seed coats: isolation, structure elucidation,
and their antiproliferative and antioxidative activities by Dong M1, He X, Liu
RH.(PubMed)
(8) Purification and Characterization of a Novel Hemagglutinin with Inhibitory
Activity toward Osteocarcinoma Cells from Northeast China Black Beans by
Dan X1, Wong JH1, Fang EF2, Chan FC3, Ng TB1(PubMed).
(9) Fermentation product of soybean, black bean, and green bean mixture
induces apoptosis in a wide variety of cancer cells by Chia JS1, Du JL, Wu MS,
Hsu WB, Chiang CP, Sun A, Lu JJ, Wang WB.(PubMed)
(10) Dietary patterns and prostate cancer risk in the National Health and
Nutrition Examination Survey Epidemiological Follow-up Study cohort by
Tseng M1, Breslow RA, DeVellis RF, Ziegler RG.(PubMed)
(11) Microarray Analyses of Genes Differentially Expressed by Diet (Black
Beans and Soy Flour) during Azoxymethane-Induced Colon Carcinogenesis in
Rats by Rondini EA1, Bennink MR.(PubMed)
(12) Dry beans inhibit azoxymethane-induced colon carcinogenesis in F344
rats by Hughes JS1, Ganthavorn C, Wilson-Sanders S.(PubMed)
(13) Consumption of black beans and navy beans (Phaseolus vulgaris) reduced
azoxymethane-induced colon cancer in rats by Hangen L1, Bennink MR.
(PubMed)
(14) Spleen and thymus histology and proliferative response of splenic cells in
rats fed raw and cooked Phaseolus vulgaris beans by Toro F1, Benshimol AL,
González Elorriaga M, Soyano A.(PubMed)