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DETERMINATION OF DISSOLVED OXYGEN IN A WATER SAMPLE BY

WINKLER’S METHOD

Expt. No. Date:

Aim:

To determine the amount of dissolved oxygen in the given water sample

Principle:

+ +
In alkaline medium, dissolved oxygen oxides to . In acidic medium, it oxides
Iodide ion to free Iodine. The amount of iodine released which can be titrated with a
standard solution of is equivalent to the dissolved oxygen.

+ −
+ + → (Brown precipitate)

+ − +
+ + → + +

− − −
+ → +

Apparatus Required:

Stoppered bottle, burette, pipette

Reagents Required:

Conc. ,� ,� , , Starch

Procedure:

Standardisation of thiosulphate

Pipette out of 20 ml of std. � . Add 5 ml of Conc. and 10 ml of 10% KI. Titrate


this against until the solution becomes straw yellow in colour. Add 3 drops of
starch solution and continue the titration. Appearance of green colour is the end point.
Repeat the titrations for concordant value.
Table: Estimation of Dissolved Oxygen

S. No. Vol. of water Burette reading Vol. of Indicator


sample (ml) Initial Final (ml)

Starch

Calculation:

Volume of Oxygen Sample (� )

Normality of the Oxygen sample ( )

Volume of solution (� )

Normality of solution ( )

As we know that, � =�

Therefore, =� /�

Amount of dissolved oxygen present in the given sample = × × �/

= ……..………………… �/
Determination of DO

Take the water sample in a stopped bottle. Add of solution followed


by of alkaline KI solution. Stopper and mix well. Allow it to stand. After 2 minutes,
again shake well and allow the precipitate to settle. Add conc. , drop by drop until
the precipitate is completely dissolved. From this solution, take and titrate against
standardized solution and add 3 drops of freshly prepared starch solution and
continue the titration till the disappearance of blue colour. Repeat the titrations for
concordant value.

Result

The amount of dissolved oxygen prese t i the gi e sa ple of ate =………………….pp

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