Human ORFeome Gateway entry vector

pENTR223-BRCA1, complete sequence

LOCUS LT742980 4887 bp DNA circular SYN 15-FEB-
2017
DEFINITION Human ORFeome Gateway entry vector pENTR223-BRCA1, complete
sequence.
ACCESSION LT742980
VERSION LT742980.1
FEATURES Location/Qualifiers
source 1..4887
/organism="Human ORFeome Gateway entry vector"
/mol_type="other DNA"
/db_xref="taxon:1958742"
/note="vector pENTR223-BRCA1;
BCCM/LMBP Plasmid collection (Ghent University,Belgium)
accession number LMBP ORF81111-F08."
regulatory complement(88..115)
/regulatory_class="terminator"
/note="T2 terminator"
regulatory 247..290
/regulatory_class="terminator"
/note="T1 terminator"
regulatory 390..487
/regulatory_class="attenuator"
/note="Gateway recombination site attL1"
CDS 488..>2586
/note="unnamed protein product; hBRCA1;
incomplete termination codon for C-terminal fusion"
/codon_start=1
/transl_table=11
/protein_id="SJX44422.1"

/translation="MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFC

KFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYA

NSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLG

TVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISL

DSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGEAASGCESETSVS

EDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSS

ALEDLRNPEQSTSEKVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERS

SPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLP

RQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQ

SPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFAR

KHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKM
LNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTGCPPNCGCA
ARCLDRGQWLPCNWADV"
regulatory complement(2587..2684)
/regulatory_class="attenuator"
/note="Gateway recombination site attL2"
regulatory complement(2705..2721)
/regulatory_class="promoter"
/note="T7 promoter"
CDS 2954..3964
/note="unnamed protein product;
streptomycin/spectinomycin
resistance (aadA)"
/codon_start=1
/transl_table=11
/protein_id="SJX44423.1"

/translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA

RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL

AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE

VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL

VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA

PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
GK"
rep_origin 4427..4829
/note="pMB1 ori"

Homo sapiens breast and ovarian cancer

sususceptibility protein 1 (BRCA1) gene,
exons 10 through 23 and partial cds
LOCUS KT152890 3523 bp DNA linear PRI 21-NOV-
2015
DEFINITION Homo sapiens breast and ovarian cancer sususceptibility protein 1
(BRCA1) gene, exons 10 through 23 and partial cds.
ACCESSION KT152890
VERSION KT152890.1
FEATURES Location/Qualifiers
source 1..3523
/organism="Homo sapiens"
/mol_type="genomic DNA"
/isolation_source="patient with breast cancer"
/db_xref="taxon:9606"
/sex="female"
/cell_type="peripheral blood"
/country="Turkey"
 gene <1..>3523
/gene="BRCA1"
mRNA join(<1..727,828..916,1017..1188,1289..1415,1516..1706,
1807..2117,2218..2305,2406..2483,2584..2624,2725..2808,
2909..2963,3064..3137,3238..3298,3399..>3523)
/gene="BRCA1"
/product="breast and ovarian cancer sususceptibility
protein 1"
CDS join(<1..727,828..916,1017..1188,1289..1415,1516..1706,
1807..2117,2218..2305,2406..2483,2584..2624,2725..2808,
2909..2963,3064..3137,3238..3298,3399..3523)
/gene="BRCA1"
/note="K1183R; BRCA1"
/codon_start=1
/product="breast and ovarian cancer sususceptibility
protein 1"
/protein_id="ALO20345.1"

/translation="FSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAE

NDIKESSAVFSKSVQRGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPC

FQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKASQEH

HLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELV

SDDEERGTGLEENNQEEQSMDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDT

MQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTS

QKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSG

SLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSD

DPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEES

VSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVM

KTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQ

GPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVH

PIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY
"
exon <1..727
/gene="BRCA1"
/number=10
variation 179
/gene="BRCA1"
/note="results in arginine to lysine substitution"
/replace="a"
gap 728..827
/estimated_length=unknown
exon 828..916
/gene="BRCA1"
/number=11
gap 917..1016
 /estimated_length=unknown
exon 1017..1188
/gene="BRCA1"
/number=12
gap 1189..1288
/estimated_length=unknown
exon 1289..1415
/gene="BRCA1"
/number=13
gap 1416..1515
/estimated_length=unknown
exon 1516..1706
/gene="BRCA1"
/number=14
gap 1707..1806
/estimated_length=unknown
exon 1807..2117
/gene="BRCA1"
/number=15
gap 2118..2217
/estimated_length=unknown
exon 2218..2305
/gene="BRCA1"
/number=16
gap 2306..2405
/estimated_length=unknown
exon 2406..2483
/gene="BRCA1"
/number=17
gap 2484..2583
/estimated_length=unknown
exon 2584..2624
/gene="BRCA1"
/number=18
gap 2625..2724
/estimated_length=unknown
exon 2725..2808
/gene="BRCA1"
/number=19
gap 2809..2908
/estimated_length=unknown
exon 2909..2963
/gene="BRCA1"
/number=20
gap 2964..3063
/estimated_length=unknown
exon 3064..3137
/gene="BRCA1"
/number=21
gap 3138..3237
/estimated_length=unknown
exon 3238..3298
/gene="BRCA1"
/number=22
gap 3299..3398
/estimated_length=unknown
exon 3399..>3523
 /gene="BRCA1"
/number=23

Synthetic construct Homo sapiens clone

CCSBHm_00023739 BRCA1 (BRCA1)
mRNA, encodes complete protein
LOCUS KR711445 2229 bp DNA linear SYN 01-JUN-
2015
DEFINITION Synthetic construct Homo sapiens clone CCSBHm_00023739 BRCA1
(BRCA1) mRNA, encodes complete protein.
ACCESSION KR711445
VERSION KR711445.1
FEATURES Location/Qualifiers
source 1..2229
/organism="synthetic construct"
/mol_type="other DNA"
/db_xref="taxon:32630"
/clone="CCSBHm_00023739"
/focus
/note="vector:pDONR223; derived from parent clone
GenBank
accession: KJ890779"
source 66..2162
/organism="Homo sapiens"
/mol_type="other DNA"
/db_xref="taxon:9606"
gene 66..>2162
/gene="BRCA1"
/db_xref="GeneID:672"
CDS 66..>2162
/gene="BRCA1"
/note="full-length ORF without stop codon"
/codon_start=1
/transl_table=11
/product="BRCA1"
/protein_id="AKI71827.1"
/db_xref="GeneID:672"

/translation="MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFC

KFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYA

NSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLG

TVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISL

DSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGEAASGCESETSVS

EDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSS
ALEDLRNPEQSTSEKVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERS

SPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLP

RQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQ

SPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFAR

KHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKM

LNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFSNMPTGCPPNCGCA
ARCLDRGQWLPCNWADV"
misc_difference 2070
/gene="BRCA1"
/note="compared to parent clone; results in T to S
substitution"
/replace="a"