®SAIGLOBAL LICENCE for ‘method for the enumeration of coagulase-positive Parker agar mediun 180 6888-1:1999 Microbiology of ood and animal feeding stuffs - Horizont Staphylococe’ (Staphylococcus aureus and other species) - Part 1: Technigue using Baird Licensee: 0 vilian Disint Date: Friday, 11 October 2013 12:40 PM Licence Agreement ns is an agreement between the end user ofthe Product Licensee") and SAl Giobal Limited, 286 Sussex Street, Sycney NSW 2000 AUSTRALIA, ABN 67 050 611 642. ). 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Licensed to Dr ian Disin on 11 Octoer Get perissin ioe INTERNATIONAL Iso STANDARD 6888-1 First edition 1998-02-15 SS Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 1: Technique using Baird-Parker agar medium Microbiologie des aliments — Méthode horizontale pour le dénombrement ges staphylocoques & coagulase positive (Staphylococcus aureus et autres espéces) — Pantie 1: Technique utilisant le milieu gélosé de Baird: Parker 10 Cini hideprohibited. (10419601) ‘onl. Copying, copy/pasing. slorage & distibution or use on network jon www Sa‘globel comficen Get permission to eapy trom or network ths publ Purenased By : Dr ian Dist, Lconsed to Dr sitian Dist on 11 Octet 2018.4 user personal ISO 6888-1:1999(E) Contents 1 Scope... 2 Normative references 3 Terms and definitions 4 Principle.. 6 Apparatus and glassware 7 Sampling. 8 Preparation of test sample. 9 Procedure. 10 Expression of results. 11 Precision.. 12 Test report... Bibliography . © 180 1900 ars reserved, Unless otherwise specfied no part of his pubicaton may be reproduce or uized in any form or by any means, elecrnic ‘or mechanical, inciuding shetocopying and microfim, without permission in uniing Fom he saben Irtematonal Organization fo Standarcizaton Case postae 65 CH-1211 Geneve 20 « Swizerand Internet iee@iso.ch Printed in Switzerandstorage & distibuiion or use on network profited (10419801), ‘ww ssllobalcomlicensing is publcat Personal Kcense ony. Copying, copy: ck29r 2013. 1 user ‘Gat permissioy to copy tom or network j : i Purchased By : Or 1so 1SO 6888-1:1999(E) Foreword 'SO (the International Organization for Standardization) is a worldwide federation of national standards bodies (|SO member bodies). The work of preparing Intemational Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. Intemational organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Eleotrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft international Standards adopted by the technical committees are circulated to the member bodies for voting, Publication as an International Standard requites approval by atleast 75 % of the member bodies casting a vote. {ntornational Standard ISO 6888-1 was prepared by Technical Committee ISO/TC 34, Agricultural food products, ‘Subcommittee SC 9, Microbiology. This first edition of ISO 6888-1, together with ISO 6888-2, cancels and replaces ISO 6886:1983, which has been technically revised, 'SO 6888 consists of the following parts, under the general tite Microbiology of food and animal feeding stutts — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 1: Technique using Baird-Parker agar medium — Part 2: Technique using rabbit plasma florinogen agar mediumion or use on network prohibited. (10818601). 2788 only. Copying, copyipating, storage & 3 Disint on 11 Octover 2013, 1 user personal lan Disint. Licensed to Dr 3 ‘Get permissca to copy tom or nem ISO 6888-1:1999(E) elso 0 Introduction 0.1 Because of the large variety of food and feed products, this horizontal method may not be appropriate in every detail for certain products. In this case, different methods, which are specific to these products. may be used absolutely necessary for justified technical reasons. Nevertheless, every attempt should be made to apply this horizontal method as far as possible. When this part of ISO 6888 is nex! reviewed, account will be taken ot all information then available regarding the extent to which this horizontal method has been followed and the reasons for deviations from this method in the case of particular products, ‘The harmonization of test methods cannot be Immediate and, for certain group of products, International Standards andlor national standards may already exist that do not comply with this horizontal method. It is hoped that when Such standards are reviewed they will be changed to comply with this part of ISO 6888 so that eventually the only remaining departures trom this horizontal method will be those necessary for well-established technical reasons, 0.2 1SO 6888 describes two horizontal methods (part 1 and part 2) for the enumeration of coagulase-positive Staphylococci among which enterotoxinogenic strains are encountered. I! is mainly concerned with Staphylococcus aureus, but also with S. intermedius and certain strains of S. hyicus. In the general case, use part 1 of ISO 6688. However, itis preferable to use the procedure described in part 2 (soe reference [1)) only for foodstuffs (such as cheeses made trom raw milk and certain raw meat products) Ikaly to be ‘contaminated by: — staphylococci forming atypical colonies on a Baird-Parker agar medium: — background flora which can obscure the colonies being sought 0.3 For the purposes ofthis part of ISO 6888, the confirmation of staphylococci is based on a positive coagulase feaction, but itis feconized that some strains of Staphylococcus aureus give weakiy positive coagulase reactions, {These later strains may be confused with other bacteria but they may be distinguished trom such other bacteria by {he use of additional tests not included in this part of ISO 6888, such as the sensitivity to lysostaphin, the production (of haemolysin, of thermostable nuclease and of acid from mannitol (see reference (2)ull oF use on network prohited. (10418891), 2013. 1 user personal Keense ony. Copying. eopy/pas ‘copy fom or network this publication ww a global. co Octo: (Get permission o n Disint on Purchased By INTERNATIONAL STANDARD iso Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 1: Technique using Baird-Parker agar medium 1 Scope This part of ISO 6888 specifies a horizontal method for the enumeration of coagulase-positive staphylococci in products intended for human consumption or feeding of animals, by counting of colonies obtained ona solid ‘medium (Baird-Parker medium) after aerobic incubation at 35 °C or 37 °C. 2 Normative references The following normative documents contain provisions which, through reference in this text, constitute provisions of this part of ISO 6888. For dated references, subsequent amendments to, or revisions of, any of these publications do not apply. However, parties to agreements based on this part of ISO 6888 are encouraged to investigate the Possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain registers of currently valid International Standards. 'SO 6887-1, Microbiology of food and animal feeding stufts — Rules for the preparation of the test sample, o inital suspension and of decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the intial suspension and of decimal dilutions. 180 7218, Microbiology of food and animal feeding stufls — General rules for microbiological examination, 3 Terms and definitions For the purposes of this part of ISO 6888, the following terms and definitions apply. 34 coagulase-positive staphylococci bacteria which form typical andlor atypical colonies on the surtace of a selective culture medium and which show a Positive coagulase reaction when the test is performed following the method specitied in this part of ISO 6888 32 enumeration of the coagulase-positive staphylococci determination of the number of coagulase-postive staphylococci found per milltre or per gram of sample when the testis carried out according to the method specified in this part of ISO 6888buon or use on network protec (10419801). Copying. copyipasting, storage & 1M or network his publication www saiglbal comlicensing on 11 Ociovor 2013.1 user personal license o ermissio to copy Purchased By: Dr Jian Disint Loonsed to Dr Jian Disk ISO 6888-1:1999(E) elso 4 Principle 4.1, Ingculaton of the surface of a soli selective culture medium, using duplicate plates, with a species quantity ‘of the test sample if the product is liquid, or with a specified quantity of the initial ‘suspension in the case of other products, Inoculation, under the same conditions, using decimal dilutions of the test sample or of the initial suspension, with {wo plates per dilution 4.2. Aerobic incubation ofthe plates at 95 °C or 87 °C) and examination after both 24 h and 48 h. 4.3. Calculation of the number of coagulase-positive staphylococci per miliitre, or per gram, of sample from the umber of typical and/or atypical colonies obtained on pates at dilution levels chosen so as to give a significant result, and confirmed by a positive coagulase test result 5 Diluent and culture media 5.1 General For curtent laboratory practice, see ISO 7218. 5.2 Diluent ‘See ISO 6887-1 and the specific standard dealing with the product to be examined. 5.3 Baird-Parker agar medium?) NOTE Commercially available media may be used. In such eases, the manufactures insttucions should be followed carefully 5.3.1 Base medium 5.3.1.1 Composition Pancreatic digest of casein 1000 Yeast extract 1.09 Meat extract 509 Sodium pyruvate 1009 L-Giycine 12.09 Lithium chloride 5.09 Agar 12.g10 22g”) Water, toa final volume of 1.000 mi 1) Depending on the gel strength of he agar. 5.3.1.2 Preparation Dissolve the components or the dehydrated complete base in the water by boiling. necessary, adjust the pH so that after sterlization tis 7,240.2 at 25 °C. 1) The temperature is agreed between the interested parties and is indicated in the test report. 2) _ The agar medium is that of Baid-Parker (see referonee (3) with the adciton of sulamezathine (see reerence (4 if the presence of Proteus is suspected,network pronbited. (1041801). 5 i é 5 z 3 Disint. Licensed to OJ Purchased By: Or (Get permission o copy iso 1S 6888-1:1999(E) Transfer the medium in quantities of 100 ml o flasks or bottles (6.5) of appropriate capacity. Sterlize the medium for 18 min at 121 °C. 5.32 Solutions 5.32.1 Potassium tellurite solution 5.3.2.1.1 Composition [Potassium teturte MK,TeO) sg —«d Water 100 ml 4) it is recommended 10 ensure belorehand that the Potassium fallunie avaiable is suitable for this test (see 5321.2) Dissolve the potassium tellurite completely in the water with minimal heating, ‘The solid should be readily soluble, Ifa white insoluble material is present in the water, discard the powder. Sterilize by filtration using 0,22 um pore size membranes. The solution may be stored atthe maximum for one month at +3 °C 42" Discard the solution if a white precipitate forms. 5.3.22 Egg yolk emulsion (concentration approximately 20 % or according to the manufacturers instructions) NOTE acommercial preparation is avaliable, it should be used Use fresh hen eggs with intact shells. Clean the eggs with a brush using a liquid detergent. Rinse them under ‘unning water, then disinfect the shells either by immersing them in ethanol (70 % volume fraction) for 30 s and allowing them to dry in the air, or by spraying them with alcohol followed by flame sterilization Proceeding under aseptic conditions, break each egg and separate the yolk from Its white by repeated transter of the yolk from one half of the shell to the other. Place the yolks in a sterile flask (6.5) and add four times their volume of sterile water. Mix thoroughly. Heat the mixture in the water bath (6,4) set at 47 °C for 2 h and leave for 18h to 24 at +3 °C +2 C to allow a precipitate to form. Aseptically collect the supernatant liquid into a fresh sterile flask for use. ‘The emulsion may be stored at +3 “C+ 2 °C for a maximum of 72h, 5.3.2.3 Sulfamezathine (sulfamethazine, sultadimidine) solution NOTE Thisis to be used only if Proteus species are suspected in the test sample. 5.3.2.3.1 Composition Suitamezathine 029 ‘Sodium hydroxide solution, (NaOH) = 0,1 moti 10 mi Water 90 mion oF use on network prohibited. (10412691) ne only. Copying, copy/pasting, storage & ctor 2013. | user personal a i 8 i 5 waved By : Drv Pur ission fo copy rom or network this publication www saigbalcomiisersing Got perms ISO 6888-1:1999(E) elso 53.232 Proparation Dissolve the sulfamezathine in te sodium hydroxide solution. Ditte to 100 mi withthe water. Stolze by firation using 0,22 um pore size membranes. ‘The solution may be stored atthe maximum for one month at +8 C+ 2 °C. 5.3.3 Complete medium 53.3.1 Composition Base medium (6.3.1) 400 mi Potassium tellurte solution (5.3.2.1) 10m Egg-yelk emulsion (6.3.2.2) 5,0 mi Sulfamezathine solution (5.3.2.3) (itnecessary) 2,5 ml 5.3.3.2 Preparation Melt the base medium, then coo! it to approximately 47 °C by means of the water bath (6.4) ‘Add, under aseptic concitons, the two other solutions (6.3.2.1 and 5.3.2.2) and if necessary (it Proteus species are Suspected in the test sample) the sulfamezathine solution (6.3.2.3), each solution being previously warmed in a water bath at 47 °C, mixing well after each addition, 5.3.4 Preparation of agar plates, Place the appropriate quantity of the complete medium (5.3.3) into sterle Petri dishes in order to obtain an agar thickness of about 4 mm, and allow to solidly. ‘The plates may be stored, prior to drying, for up to 24 hat +3 «C+ 2°C. NOTE The manufactures instructions shoul be followed concerning the storage period for industally prepared plates. Before uso, dry the pistes, preferably will the lids off and the agar surtace downwards, in an oven set at @ temperature between 25 ‘C and 50 °C, until the droplets have disappeared from the surface of the medium, 5.4 Brain-heart infusion broth 5.4.1 Composition Enzymatic digest of animal tissues 1008 Dehydrated calf brain infusion 1259 Dehydrated beet hear infusion 5.09 Glucose 209 Sodium chloride 509 Disodium hydrogenphosphate, aniydrous (NagHPO,) 259 Water 1.000 mi0419601), 1298 & dstbuton or use on network pri ted. perconal conse only. Copying, copypasting, se (0n11 Octeber 2013. 1 user Got permission to copy irom ov he Jn Dis LUcansed o Or Iso 1SO 6888-1:1999(E) 5.4.2 Preparation Dissolve the components or the dehydrated complete medium in the water, heating if necessary, Adjust the pH so that after sterilization itis 7,4 + 0,2 at 25 °C, ‘Transfer the cuiture medium in quantities of 5 mito 10 ml to tubes or bottles (6.5) of appropriate capacity Steriize the medium for 15 min at 121 °C, 5.5 Rabbit plasma Use commercially avaiiable dehydrated rabbit plasma and rehydrate it according to the manufacturers instructions. |i dehydrated rabbit plasma is not available, dilute one volume of fresh sterile rabbit plasma with three volumes of sterile water. ‘Add EDTA (ethylenediaminetetraacetic acid) solution to give 0,1 % EDTA in the rehydrated or diluted plasma, if Potassium citrate or sodium citrate has been used as the plasma anticoagulant 9), Unless stated by the manufacturer, the rehydrated or diluted plasma shall be used immediately. Before use, test each batch of plasma with coagulase-positive strains of staphylococci and strains of coagulase- negative staphylococci 6 Apparatus and glassware NOTE Disposable apparatus is an acceptable alternative to reusable glassware iit has suitable specifications: Usual microbiological laboratory equipment (see ISO 7218) and, in particular, the following, 6.1 Apparatus for dry sterilization (oven) and wet sterilization (autoclave) See ISO 7218, 6.2. Incubator, for maintaining the inoculated media, plates and tubes within the temperature range 35 °C +1 °C or 37 C£1°C. 6.3 Drying cabinet or incubator, capable of being maintained at between 25 °C + 1 *G and 50 ‘C+ 1°C, 6.4 Water bath, or similiar apparatus, capable of being maintained at 47 °C +2 °C. 6.5 Test tubes, flasks or bottles with screw caps, of appropriate capacity, for sterilization and storage of culture ‘media and incubation of liquid media; in particular, sterile haemolysis tubes, or round-bottom bottles of approximate dimensions 10 mm x 75 mm, 66 Petri dishes, storile, made of glass or plastic. 6.7. Straight wire (see ISO 7218) and Pasteur pipette. 68 Total-delivery graduated pipettes, of nominal capacities 1 mi, 2 ml and 10 ml, graduated in 0,1 ml, 0,4 mi ‘and 0,5 mi divisions, respectively. Oxalated or heparinized plasma does not require EDTA (see relerence (5)Proibited. (10419801), pasting, storage & dletibution or use on network personal conse nly. Copying, copy network, n Dist. Licensed te Dr Jian Dsint on 11 Ociber 2013. + user Purchased By : Oral publication wera sagobal.comicensing Gt perissn to copy om ISO 6888-1:1999(E) e1so 6.9 Spreaders, sterile, made of glass or plastic. 6.10 pH-meter, capable of being read to the nearest 0,01 pH unit at 25 C, enabling measurements to be made which are accurate to + 0,1 pH unit 7 Sampling ‘Sampling is not part of the method specified in this part of ISO 8888. If there is no specific International Standard dealing with sampling of the product concerned, it is recommended that the parties concerned come to an agreement on this subject. "is important that the laboratory receive a sample which is truly representative and has not been damaged or ‘changed during transport or storage. 8 Preparation of test sample Prepare the test sample in accordance with the specific International Standaré appropriate to the product Concerned. If there is no specific International Standard available, it Is recommended that the parties concerned ‘come to an agreement on this subject. 9 Procedure 9.1. Test portion, initial suspension and dilutions ‘See ISO 6887-1 and the specific standard appropriate to the product concemed, 9.2 Inoculation 9.2.1 Transter, by means of a sterile pipette (6.8), 0,1 ml of the test sample if liquid, or 0,1 mi of the initial Suspension (10 dilution) in the case of other produets, to each of two agar plates (5.3.4). Repeat the procedure for ‘the 10°? dilution and for further decimal dilutions it necessary, 9.2.2 I, for certain products, it is desirable to count low numbers of coagulase-positive staphylococci, the limits of detection can be raised by a factor of 10 by inoculating 1,0 ml of tha test sample if liquid. oF 1,0 ml of the initial Suspension for other products, either on the surface of one large agar plate (140 mm) or on the surface of three small agar plates (90 mm). In both cases, prepare duplicates by using two large platas or six small ones. 9.2.3 Carefully spread the inoculum as quickly as possible over the surface of the agar plate, trying not to touch the sides of the dish, using the spreader (6.2). Allow the plates to cry with their lids on for about 15 min at laboratory temperature. 9.3 Incubation lnvert the plates prepared according 10 9.2.3 and incubate them for 24h+2h then re-incubate for a further 24m * 2 hin the incubator (6.2) at 35 °C or 37 *C4) 9.4 Selection of plates and interpretation 8.4.1 After incubation for 24 h 2h, mark on the bottom of the plates the positions of any typical colonies present (see note 1), 4) The temperature is agreed between the interested parties and is indicated inthe test reportproniited. (10418601) ly. Copying, copy'pasting, slorage & stibution or use on network on wi. seigiobalconvicensing 37 Disint. Licensed to Dr lan Disin on 11 Octsber 2013.1 user personal conse on Purchased By : Dr Ji ‘Get parmissin to copy from or network his pul Iso 1SO 6888-1:1999(E) Ro-incubate all plates at 35 °C or 37 °C) fora further 24 h = 2h, and mark any new typical colonies. Also mark any atypical colonies present (see note 1), ‘Take for enumeration only those plates (see note 2) that contain at the maximum 300 colonies with 150 typical andior atypical colonies at two successive dilutions. One of the plates shail contain at least 15 colonies. Select for confirmation (9.5) a given number A (in general 5 typical colonies if there are only typical colonies, or 5 atypical Colonies if there are only atypical colonies, or 5 typical colonies and 5 atypical colonies i both types are present, from each plate). It there are less than 15 typical andlor atypical colonies present on plates inoculated with undiluted liquid product or the lowest dilution of other products, itis possible to make an estimated count as described in 9.4.3 and 10.2. NOTE 1 Typical colonies are biack or grey, shining and convex (1 mm ta 1,5 mm in diameter after incubation for 24 h and 1,S mm to 2.5 mm in Giameter aftor incubation for 48h) and surrounded by a clear zone. After incubation for at least 24°h an ‘opalescent ring, immediately in contact withthe colonies, may appear in this clear zone. ‘Atypical colonies may present one ofthe following morphologies: ) shining black colonies with or without a narrow white edge; the clear zone is absent or barely visible and the opalescent "ing is absent or hardly visible; 1b) grey colonies tree of clear zones. ‘Atypical colonies are formed mainly by strains of coagulase-positve staphylococ! contaminating, for example daiy products Shrimps and giblets, They are loss often formed by strains of coagulase-posktve staphylococci contaminating otter products, NOTE 2 Bacteria belonging to genera other than staphylococci may give colonies with an appearance similar to Staphylococci. Microscopic examination of Gram stain, before coniimation, will enable the distinelion of other genera trom staphylococci 9.4.2 Ifa 1,0 ml inoculum was spread over three plates (see 9.2.2), treat these plates as one in all subsequent ‘counting and confirmation procedures. 9.43 To make an estimated count of lower numbers of coaguiase-positive staphylococci, retain all plates that contain any typical and atypical colonies. Select all such colonies for confirmation within the limits set out above. 9.5 Confirmation (coagulase test) From the surface of each selected colony (9.4), remove an inoculum with a sterile wire (6.7) and transfer it to a tube ‘bottle of brain-heart infusion broth (5.4). Incubate at 35 °C or 37 °C) for 24h =2h, Aseptically ad 0,1 mi of each culture to 0,3 ml of the rabbit plasma (6.6) (unless other amounts are specified by the manufacturer) in sterile haemolysis tubes or bottles (specified in 6.5), and incubate at 25 °C or 37 "C3 By tilting the tube, examine for clotting of the plasma after 4 n to 6 h of incubation and, it the test is negative, re-examine at 24 h of incubation, or examine at the incubation times specified by the manufacturer. Consider the coagulase test to be positive if the volume of clot occupies more than half of the original volume of the liquid ‘As @ negative control, for each batch of plasma, add 0,1 mi of sterile brain-heart infusion broth (6.4) to the recommended quantity of rabbit plasma (6.5) and incubate without inoculation. For the test to be valid, the control plasma shall show no signs of clotting. 5) _ The temperature is agreed between the interested parties and is indicated in the test report#€r personal Keense ony. Copying, copy/pasin, storage & cstbution or use on network prohibited. (10413601), Get permission copy trom of network this pubication www saiglobal comicensine 7 Dsint on 11 October 2013.1 wrehased By : Or Jilin Dist. Lieaneedto Dr. ISO 6888-1:1999(E) iso 10 Expression of results 10.1 General case 10.1.1 Calculation of the number « of coagulase-positive staphylococel identified for each plate selected Calculate, for each of the plates, the number « of identified coagulase-posttive staphylococci, according to the equation: Ye Be = Exe + TE Xe where 4,_ is the number of typical colonies submitted to the coagulase test (9.5); ‘Ape is the number of atypical colonies submitted to the coagulase test (9.5): ‘bes the number of typical colonies which have been shown to be coagulase-positve. ‘nq 8 the numberof atypical colonies which have been shown to be coagulase-posiive. q_'8 the total number of typical colonies seen on the plate (8.4); yo i the total numberof atypical colonies seen on the plate (9.4) Round otto a whole number (see ISO 7218) 10.1.2 Calculation of the number \V of identified coagulase-positive staphylococci present in the test Portion For those dishes containing at the maximum 300 colonies, with 150 typical and/or atypical colonies at two Consecutive dilutions, calculate the number of coagulase-positive staphylococci for each dish as specified in 10.1.1 and calculate, as a weighted mean from the two successive cilutions, the number N of identified coagulase-positive staphylococci present in the test sample, using the following equation ve be Von + OF ng) where Les the sumot the eoagulase-postve stphylococal colores identified on al the dishes selected V-Isthe volume of inoculum on each ash, in mites; ‘ny Is the numberof dishes selected athe first dilution; ‘tg is the number of dishes selected at he second dition: 4 ste elution rate corresponing tothe fst dilution selected (the intial suspension isa dition). Found off the calelated results to two significant figures (see ISO 7218). Report the resut as the numberof coagulase-positve staphylacocc per mille (quid products) or per gram (other Products), expressed asa number betwoen 1,0 and 98 inclusive multiplied by 10" where «i tho appropriate power 10.1.3 Example AA count of a product after inoculation with 0,1 mi of praduct gave the following resultssonal Keense onl. Copying, copyipasing, slorage& asirbution or use en network rahe. (10419601), fn 11 Ocober 2013. 1 user pe Get permission 1o copy tom or network this publication wanw eagiooa con : } Jn Dis Purchased By : Dr J ISO 6888-1:1999(E) els0 "the inooulation has been performed with 1 mi of sample, report the result as follows: less than 1 coagulase-positive staphylococcus per mililtre (liquid products); — less than 1/«d coagulase-positive staphylococcus per gram (other products). 11 Precision See ISO 7218, 12 Test report The test report shall specity — allinformation necessary tor the complete identification of the sample; — the sampling method used, if known; — the test method used, with reference to this part of ISO 6888; — the incubation temperature used; — all operating detalis not specified in this part of ISO 6888, or regarded as optional, tagether with details of any Incidents which may have influenced the test results; — the results obtainea,prohibited. (10419801), iy. Copying, copy/pasting. sorage & ditbution or use on network 4 t ; 5 11 Ociobor 2013. 1 user personal icense on [permission Io op rom or network this publ Gel ‘Purchased By : Dr Jian Disint. Licenised to Dr iin Disa. e180 ISO 6888-1:1999(E) uy (2) 8 [4] 6) Bibliography 'SO 6888-2, Microbiology of food and animal feeding stulls — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 2: Techmique using rabbit plasma fibrinogen agar medium. KLoos W.E. Systematics and the natural history of staphylococci. In: Staphylococci, J. Appl. Bacteriol. Symp, ‘Suppl, 68, 1990, pp. 25 s -37 s; and Bergey's Manual of Determinative Bacteriology, 9!" edn, 1994. BAIRO-PAAKER, A.C. J. Appl. Bacteril., 25 (1), 1962, pp. 12-19. Suh, B.A. and BAIRD-PARKER, A.C. J. Appl, Bacteriol, 27 (1), 1964, pp. 78-82 BAIRO-PARKER, A.C. The Staphylococci, (ed. Cohen, J.0,), Wiley-Interscience, New York, London, 1972, p.11,2iso ISO 6888-1:1999(E) (0864801) povayosd yomou uo asm 0 uounaNSpp ¢ ‘Bursu0>q 00 ego/ies an Loneojqad sh ebents doo 01 assed a5) 1980 1 "£102 89009 44 uO wg U ICS 07.100.30 I 20.01 PEELED "SIC UBHHR 40: Aa BosEYaINg rie based on 11 pages