Clinical Chemistry Analyzer

Daily Guide
Ref: RAB275CEN
Daily Guide

Daily Guide
Ref: RAB275CEN
Contents

1. Revisions..................................................................................................1

2. Software Icons Description..............................................................2

3. Safety Precautions...............................................................................3
3.1. Warning and Precautions..............................................................................3
3.2. Environment................................................................................................3
3.3. Labels and Connections...............................................................................4
3.4. Power Requirements....................................................................................6
3.5. Electronic and Moving Parts..........................................................................6
3.6. Biological Risk.............................................................................................7

4. Workflow..................................................................................................8
4.1. Sample Materials.........................................................................................8
4.2. Start of Day.................................................................................................8
4.3. Reagent Cassette Management...................................................................10
4.4. Calibration and Control...............................................................................12
4.5. Patient Samples.........................................................................................17
4.6. End of Day................................................................................................22
4.7. To Stop in Emergency During Analysis.........................................................26

5. Flags........................................................................................................27
5.1. Error Flags.................................................................................................27
5.2. Error Code for ISE......................................................................................29

Daily Guide i
Ref: RAB275CEN
Daily Guide ii
Ref: RAB275CEN
Revisions

1. Revisions

YOUR LOCAL REPRESENTATIVE:

Index Reference Software Version Date
A RAB275AEN 2550494104 September 2009
B RAB275BEN 1.06 (2550497106) January 2010
C RAB275CEN 1.07 (2550xxx107) February 2011

This document applies to the latest software version listed and higher versions.
When a subsequent software version changes the information in this document, a new
electronic issue (CD-ROM and/or online help) is released and supplied by
HORIBA Medical.
To update a paper document, please contact your local HORIBA Medical representative.

■ This instrument is intended for professional use only.

■ The user manual is supplied on the CD-ROM and must be consulted prior
to operating the instrument.
■ Please refer to the user manual for potential hazards and risks, technical
specifications and performance data.
■ This document is only a guide for daily usual operations and cannot
replace the instrument user manual.

If a problem occurs during the instrument operation, refer to the user manual
instructions for recommendations. If the problem persists, please contact your
local HORIBA Medical representative.

Documentation CD-ROM instructions

To view or to print this user manual, place the CD-ROM into any computer CD-ROM
drive, and follow the instructions.

Daily Guide 1
Ref: RAB275CEN
Software Icons Description

2. Software Icons Description

Start: to run patient's sample, calibration or control. Shutdown: to open Shutdown window.

Pause: to stop sampling. Back: to go to previous screen.

STAT: to run an emergency sample. Print screen: to print data / to send data to the host.

Inventory: to register reagents. Alarm: to open Alarm menu.

MSDS: to open the reagent online help. Help: to open the Pentra C200 online help.

Audible Alarm: to stop the sound alarm in progress. Keyboard: to display Virtual Keyboard screen.

Instrument status

Daily Guide 2
Ref: RAB275CEN
Safety Precautions
Warning and Precautions

3. Safety Precautions

3.2. Environment
3.1. Warning and Precautions

The operation of the Pentra C200 should be restricted to indoor location use only!
Instrument is operational at an altitude of maximum 2000 m (6562 ft).
Work safety reliability and general characteristics are guaranteed by HORIBA Medical
under the following conditions: The instrument is designed for safety from voltages surges according to INSTALLATION
CATEGORY II and POLLUTION DEGREE 2 (IEC 61010-1).
■ User manual must be entirely read, and personnel trained by a HORIBA Medical
representative before attempting to operate the instrument. Please contact your local representative for information regarding operation locations,
■ The user always operates with full knowledge and appreciation of instrument when it does not comply with the recommended specifications.
warnings and alarms.
■ Always refer to labelling and HORIBA Medical instructions in order to avoid to
compromise system integrity.
This instrument must be operated as instructed in the user manual. Any other use might
compromise system integrity and might be hazardous for the operator.
This instrument complies with Standards and Directives named in the Declaration of
Conformity. The latest version of the Declaration of Conformity for this instrument is
available online at www.horiba.com.

■ The reagents and accessories stipulated by HORIBA Medical have been

validated in accordance with the European Directive for in vitro medical
devices (98/79/EC).
■ The use of any other reagents and accessories may place at risk the
performance of the instrument, engaging the user's responsibility. In this
case, HORIBA Medical takes no responsibility for the device nor for the
results rendered.
■ Disposable gloves, eyes protection and lab coat must be worn by the
operator.
■ Local or national regulations must be applied in all the operations.
■ Mobile phones should not be used in proximity of the instrument.
■ All peripheral devices should comply with relevant standards.

Daily Guide 3
Ref: RAB275CEN
Safety Precautions
Labels and Connections

3.3. Labels and Connections 3.3.2. Power Supply Connection

The Power switch and Power supply connection should always be accessible!
When positioning the system for operational use, leave the required amount of
space for easy accessibility to these items.

3.3.1. Serial Number Label

The main power switch is located on the right-hand side of the instrument.

The serial label is located at the back side of the instrument.

To replace fuses, refer to Maintenance and Troubleshooting > Troubleshooting >

Operation Problems > Power Problems chapter of the user manual.

3.3.3. Fluidic System Connections

A color code is used for connections, tubing and tank identification. Blue color is used
for distilled water whereas red is used for waste.

Daily Guide 4
Ref: RAB275CEN
Safety Precautions
Labels and Connections

3.3.4. Peripherals Connections

1 = Screen brightness adjustment

1 = Liquid level sensor connector 2 = Cable lock hook (PC and USB key)
2 = Water supply for sample pump unit, reagent pump unit and wash pump unit (PFA tube) 3 = USB connections for external units
3 = Water supply for probe and mixer wash towers (silicon tube) (keyboard, printer, mouse ...)

4 = ISE concentrated waste (silicon tube) 4 = Host connection

5 = Waste water (silicon tube)

■ PFA tube for water supply (2) needs to be tightened by hand without any
tool.
■ Water tank needs to be kept at the instrument level and silicon tube (3) is
cut to be as short as possible between connector and tank.
■ Waste tank needs to be located below the instrument and loops must be
avoided between connector and tank. Cut the silicon waste water tube to
avoid loops. Whenever it is possible, leave USB port n°4 available for technical operations.
If it is not possible, use the USB port n°4 for mouse (option) or keyboard (option).

ISE concentrated waste can be separated from other non concentrated waste
for special disposal of contaminated liquid.
3.3.5. Supplied USB Key

The USB key is preloaded with the relevant data pertinent to the system.
Dispose of waste according to your local/national guidelines for biohazard
waste disposal.

Daily Guide 5
Ref: RAB275CEN
Safety Precautions
Power Requirements

3.3.6. Warnings, Cautions and Biological Risks Labels Labels Warning Location

Warning, biological hazard Dust pod

Labels Warning Location

Risk of electric shock Power supply inlet

Covers of probe unit, mixer

Do not touch moving parts
unit, cuvette loading unit 3.4. Power Requirements

The tank contains hazardous

Waste tank
material

■ Power supply: from 100 - 120 V to 200 - 240 V (+/- 10%), 50 Hz to 60 Hz

Contains hazardous materials Fluidic system connection, ■ Maximum power consumption: 600 VA
(serum, plasma or urine) reaction tray cover
■ Maximum heat output: 1877 BTU/h (1980 kJ/h)

Risk of injury.
Turn the power OFF before
Halogen lamp cover, pump Fuses characteristics:
unit panel, ISE module panel
opening this panel.
Type Quantity Characteristics
Warning, hot surface.
Ceramic tube fuse 2 6.3 A / 250 V
Risk of personal injury due to hot
surfaces within the instrument. Detector unit
Wait for the lamp to cool down
before proceeding.
General warning, caution, risk of
danger.
Risk of personal injury during the
reagent and sample container 3.5. Electronic and Moving Parts
Sample and reagent
unit cover operation (hands/
container unit, cuvette
fingers). Ensure that the unit
loading unit, reaction unit
cover closure is done cautiously.
Risk of personal injury during
cuvette loading unit operation The following parts must not be handled or checked by the user:
(hands/ fingers). ■ Electrical Power supply
■ Electronic circuit boards

Daily Guide 6
Ref: RAB275CEN
Safety Precautions
Biological Risk

Troubleshooting > Maintenance > Other Procedures > To Decontaminate the Instrument
Operator injury may occur from an electric shock. Electronic components can chapter of the user manual to perform the instrument cleaning and decontamination
shock and injure the user. Do not dismantle the instrument nor remove any procedure.
components (covers, doors, panels and so on) unless otherwise instructed
within this document.
Danger of explosion if battery is not replaced correctly! When replacing the
battery, always use the same and/or equivalent type recommended by the
manufacturer. Dispose of used batteries according to the manufacturer's
specific instructions.

Moving parts: It is strictly forbidden to disable sensors as it may cause

operator injuries. Protection covers must not be opened during instrument
operations. Opening the doors and covers during instrument operations
causes the instrument emergency stop.

3.6. Biological Risk

Consider all specimens, reagents, calibrators, controls, etc. that

contain human specimen extracts as potentially infectious! Use
established, good laboratory working practices when handling
specimens. Wear protective gear, gloves, lab coats, safety glasses
and/or face shields, and follow other biosafety practices as specified
in OSHA Blood borne Pathogens Rule (29 CFR part 1910. 1030) or
equivalent biosafety procedures.

All accessible surfaces of the instrument can be potentially contaminated by

human specimens. Disposable gloves and lab coat must be worn by the
operator. Local and national regulations must be applied in all the operations.

The manufacturer uses disinfectant product for instrument decontamination and highly
recommends it to decontaminate your instrument. See Maintenance and

Daily Guide 7
Ref: RAB275CEN
Workflow
Sample Materials

4. Workflow

4.2. Start of Day

At the beginning of a day of work, the instrument wakes from sleeping mode.
4.1. Sample Materials When the instrument is in sleeping mode, the green LED on the right of the screen blinks
and the screen is black. During automatic wake up the instrument performs initialization.
When the preparation process is finished the status changes from "START UP" to
"READY" and the pop-up disappears.
Samples such as serum, plasma, urine and homogeneous liquid are to be used on the
Pentra C200.

Ensure that a sufficient sample volume without air bubble or foam is placed on 4.2.1. To Log on the Instrument
the instrument whatever the sample type (calibrator, control, patient sample).

Access: Main menu > Shutdown

Refer to each reagent notice to determine the sample volume required (see Performance
on Pentra C200 > Sample Volume paragraph) and take into account the following dead When the instrument wakes from sleeping mode the last user is still logged in. To
volumes: change user, the logout procedure must be followed.
■ primary tubes: 1 mL, 1. Press the Shutdown button in the generic toolbar at the top of the main screen.
■ sample cups: 100 µL.
For any further information or special care regarding samples such as pretreatment or
sample stability, please refer to the corresponding reagent notice.

A pop-up with several choices (Sleep, Power Off, Logout, Restart, Cancel) is
displayed.

2. Press Logout.
A pop-up is displayed to confirm system logout.

Daily Guide 8
Ref: RAB275CEN
Workflow
Start of Day

3. Press OK to confirm system logout or Cancel to cancel the ordered logout. 7. Press Login.
A pop-up is displayed for user confirmation.

8. Press OK.
9. Press the Back button in the generic toolbar to enter the main screen.

4.2.2. Checks Before Measurements

Before starting measurements, several checks need to be performed to avoid shortage

or overflow:
1. Check that enough cuvettes are available on the instrument before starting
measurements.
Refer to the worflow chapter of the user manual (see Worflow > Start of Day > To
Check Cuvettes chapter).
2. Check the number of acceptable used cuvettes in the dust pod and empty if necessary.
1 = User name dropdown list Refer to the worflow chapter of the user manual (see Worflow > Start of Day > To
Check Dust Pod chapter).
2 = Password field 3. Check that a USB key is connected to the instrument (option).
3 = Login button Refer to the worflow chapter of the user manual (see Worflow > Start of Day > To
Check USB Key chapter).
4 = Logout button 4. Switch on the printer (option).
Refer to the worflow chapter of the user manual (see Worflow > Start of Day > To
Switch On the Printer chapter).
4. Wait until the Login button is active and press the arrow from the users dropdown
list to display users.
5. Select the user.
6. Type your password in the password field.

Daily Guide 9
Ref: RAB275CEN
Workflow
Reagent Cassette Management

4.3. Reagent Cassette Management 3. Check reagents for the tests you intend to run on the instrument.
Total displays the total number of tests that can be performed on the instrument with
the reagents registered on board.

If an ISE module is installed (option), keep cassette position 15 for a 30/10

open cassette (No 801, Name DIL1) containing distilled water (R1) and
4.3.1. To Check Reagent Availability Standard 1 solution (R2). Change distilled water and Standard 1 solution
every week. Clean the cassette, dry it and refill it every week.

Access: Inventory > Inventory (tab)

The Inventory tab allows you to check reagent availability and volumes. Volumes are 4. Check the Inner (R2) tab.
deduced from the last use of reagent cassettes. The number of tests available is in
accordance with the method configured with the reagent. For new cassettes, theoretical
values are displayed and actualized after the first pipetting.

1. Open the Outer (R1) tab. 4.3.2. To Load Cassettes

It displays information concerning reagents located on the outer position on the
reagent tray.

■ Red background is displayed when the validity or the stability is out of

date. If several cassettes of the same reagent are installed on the reagent tray, do
■ Orange background is displayed when reagent volume is low (less than not forget to run a new calibration when a new cassette is used.
10%).
■ Green background is displayed when reagents are available for analysis.

1. Open the main cover.

2. Check that reagents needed for your tests are registered on one of the 15 positions 2. Remove the Sample and Reagent Container Unit (SRCU) cover.
on the tray.
3. Remove the reagent and sample trays from the SRCU.
If cassettes needed for tests are not registered, you can load and register 4. Detach the sample tray by pulling it up.
new cassettes by following the cassettes loading procedure and the 5. Load the cassettes in any available position of the reagent tray.
cassettes registration procedure (see Workflow > Reagent Cassette
Management > To Load a Cassette chapter and Workflow > Reagent
Cassette Management > Cassette Registration > To Register a Cassette
chapter).

Daily Guide 10
Ref: RAB275CEN
Workflow
Reagent Cassette Management

4.3.3. Cassette Registration

Ideally, cassettes should be placed in positions which balance the tray.

4.3.3.1. Cassette Management Overview

6. Put back the reagent and sample trays in the SRCU by inserting the two guide pins Access: Inventory > Reagent Tray (tab)
in the two bigger holes of the reagent tray.
7. Remove the caps from the cassettes.
8. Press on the center of the trays to make sure the trays are correctly placed.
9. If necessary, remove bubbles with a pasteur pipette.
10. Put back the SRCU cover in correct position.
11. Check that the probe has no obstacle on its way.
12. Close the main cover.

Do not forget to register the cassettes after their loading (see Workflow >
Reagent Cassette Management > Cassette Registration > To Register a
Cassette chapter).

1 = Reagent tray representation

2 = Cassette information area

To get information about a cassette, press the concerned circle on the tray
representation and information about the cassette compartment is displayed in the
cassette information area.

Daily Guide 11
Ref: RAB275CEN
Workflow
Calibration and Control

4.3.3.2. To Register a Cassette 2. Check the identification of the selected cassette on the cassette identification area.
3. Press Delete.
Access: Inventory > Reagent Tray (tab)
4. Press Save to validate the deletion.
A pop-up is displayed and allows you to confirm or to cancel the deletion.
■ To proceed to cassette registration, reagents must already be registered on the
instrument ( Main menu > Services > System configuration > Reagent (tab) ),

All the cassettes registered on the tray can be deleted by pressing Delete
■ Reagents provided by HORIBA Medical are already registered on the All button.
instrument,
■ Only reagents for open channel must be registered by the user,
■ Only level 2 users (L2) are allowed to register new reagents.

■ Open cassettes must be registered on the instrument (see User Manual: Workflow >
Reagent Cassette Management > To Register an Open Cassette chapter for open
cassette registration),
■ Reagent cassettes must be loaded on the instrument.
4.4. Calibration and Control
1. Press Barcode Scan to register new cassettes.
The instrument checks the cassettes available on the instrument.

2. Press Save to save cassette registration. Before running any sample, the user needs to check daily calibration and
control validity for the concerned tests.
If the instrument encounters a barcode scan problem for a cassette, follow the
procedure described in the troubleshooting chapter of the user manual. See
Maintenance and Troubleshooting > Troubleshooting > Undetected Cassette
chapter. 4.4.1. To Calibrate ISE

4.4.1.1. To Order an ISE Calibration

4.3.4. To Delete a Cassette
Access: Main menu > Order > Selection (tab)

Access: Inventory > Reagent Tray (tab)

1. Open the main cover.

1. Select the cassette position by presssing the corresponding circle on the tray 2. Remove the Sample and Reagent Container Unit (SRCU) cover.
representation. 3. Place Standard 2 solution (2 mL) for ISE calibration in a tube.

Daily Guide 12
Ref: RAB275CEN
Workflow
Calibration and Control

4. Load the tube at the first position on the sample tray. 4.4.2. Check of Calibration and Control Status
5. Close the SRCU cover.
6. Close the main cover.
7. In Main menu > Order > Selection (tab) , register ISE calibration: 4.4.2.1. Test Color Code
a. Select position 1.
b. Select ISE Calibration from the Type dropdown list. Access: Main menu > Order > Selection (tab)
c. Press Save.
All tests are displayed on the Selection screen. According to the sample category
selected, they are associated with a color code corresponding to the calibration and
8. In Main menu > Services > Customer Services > Sequence (tab) , press ISE control status.
Calibration button.

■ ISE calibration validity must be checked after each calibration.

■ This calibration is stable for eight hours.

4.4.1.2. To Check ISE Calibration Validity

Access: Main menu > ISE Calibration

1. Press ISE Calibration button from the main screen.

2. Check the Error Code field.

■ If error code is 0000, the calibration is valid and the samples can be run. Remove
the Standard 2 sample tube (position 1) and discard it according to your local/
national guidelines for biohazard waste disposal. Delete the worklist.
■ If error code is different than 0000, an error occured during the calibration. Check
the Standard 2 sample tube position in the sample tray and rerun an ISE
calibration. If the error persists, refer to the troubleshooting chapter of the user
manual (see Maintenance and Troubleshooting > Troubleshooting).

Daily Guide 13
Ref: RAB275CEN
Workflow
Calibration and Control

1 = Worklist 2. Press the first position in the worklist to select position 1.

2 = Sample details and orders 3. Check the tests available on the instrument and their status which is associated with
a color code.
3 = Button to expand or collapse the list

■ If several cassettes of the same reagent are installed on the reagent

tray, do not forget to run a new calibration when a new cassette is used.
■ Always run controls for a test after each new calibration and before
If a cassette used for a test is not on board, the test is not displayed.
samples.

Color Status Action

Register the reagent and the cassette
No test The test is not displayed. used for the concerned test.
Turns to
Calibration is not valid or User needs to run a new calibration.
Red background:
required. Turns to when selected
4.4.3. To Order Calibration and Control
User needs to run a new control.
Yellow background: Control error or required.
Turns to when selected
Select the Mask tab (in Main menu >
The test is masked and will Order ) and unselect the mask for the
Grey Background:
not be run. concerned test. 4.4.3.1. To Order Calibration
Turns to
The test is available for Access: Main menu > Order > Selection (tab)
No background: measurements. Calibration Turns to when selected
and controls are valid.
If using different calibrators, follow the procedure given below as example for each
calibrator registration. Modify Sample field, MS field and No. field according to your needs.
4.4.2.2. To Check Calibration and Control Validity
1. Select an empty position in the worklist.
Access: Main menu > Order > Selection (tab) 2. Select Multistandard (example) from the Type dropdown list.
3. Select the sample category (Common, Serum, Plasma or Urine) from the Sample
1. If the worklist is not empty: dropdown list: Common (example).
a. Press Delete All.
4. Select MCAL (example) from the MS dropdown list.
b. Press Save to validate the deletion.
5. Select Full for full calibration or the calibration point number for one point calibration
A pop-up is displayed and allows you to confirm or to cancel the deletion.
from the No. dropdown list.
6. Select the tests to calibrate.

Daily Guide 14
Ref: RAB275CEN
Workflow
Calibration and Control

7. Press Save. 2. Press the >> button below the worklist.

An automatic SID (950000XX) is assigned to the calibrators. Instrument automatically 3. Load calibrators and controls according to the worklist.
assigns the appropriate positions for multipoint calibrations in accordance with the a. Remove the Sample and Reagent Container Unit (SRCU) cover.
registered calibration for each test.
b. Load sample cups with adaptor on the sample tray.

■ Ensure that a sufficient sample volume without air bubble or foam is

If using a calibrator with a serial dilution registered, select Serial Dilution from placed on the instrument (refer to Workflow > Sample Materials
the Type dropdown list. chapter).
■ Caps must be removed from the sample cups as they may hit the
Sample and Reagent Container Unit (SRCU) cover.

4.4.3.2. To Order Control

4. Close the SRCU cover.

Access: Main menu > Order > Selection (tab)

5. Check that the probe has no obstacle on its way.
6. Close the main cover.
1. Select an empty position in the worklist.
2. Select Control from the Type dropdown list. 4.4.3.4. To Start Measurement
3. Select the appropriate control from the Control dropdown list.
4. Select the tests. Before you start measurements, the following conditions must be fulfilled:
5. Press Save. ■ Enough new cuvettes must be loaded on the instrument.
An automatic SID (970000XX) is assigned to the controls. ■ Dust pod must allow to store cuvettes used during measurements.
■ Reagents, diluents and wash solutions are on board and enough for ordered
measurements.
■ Check that the water tank is full, if not fill it up with distilled water.
4.4.3.3. To Load Calibrator and Control ■ Check that the water tubing end stands in the bottom of the water tank.
■ Check that the waste tank is empty, if not empty it.
■ The cover of the Sample and Reagent Container Unit (SRCU) must be closed.
Access: Main menu > Order > Selection (tab)
■ The instrument main cover must be closed.
■ The STAT unit must be closed.

1. Press Start button for more than two seconds.

Place calibration and control solutions in sample cups.

1. Open the main cover.

Daily Guide 15
Ref: RAB275CEN
Workflow
Calibration and Control

The instrument displays the start time and the estimated finish time for
If not enough cuvettes on board, the message "Shortage of cuvette or measurements in the main screen.
disposal space for all orders. Continue the measurement?" is displayed.

The following pop-up message may be displayed: "Conduct DTR automatic 4.4.4. To Check Calibration and Control Results
measurement?".

2. Press OK. Access: Main menu > Run

The autogain measurement of the spectrophotometer is only performed for
the first measurement of the day.
A pop-up is displayed: "Preparing for measurement start".
During measurements and when run is complete, the Run screen displays calibration
and control results.
1. Check for calibrations validity in the Calibration tab.
■ If an error is displayed for a calibration, the calibration and the controls
associated must be rerun.
■ If no error is displayed, check for the controls validity.

■ If a cassette has been removed or if a new reagent lot number has

2. Check for controls validity in the Control tab.
been loaded since the last barcode scan, the following pop-up
message is displayed: "The reagents placed on the SRCU do not match ■ If an error is displayed for a control, the control must be rerun.
with the registered information. Start measurement?". ■ If no error is displayed, samples can be run for the test.
■ If a cassette has been removed, the pop-up message displays
"Cassette removed" and indicates the cassette position and the
reagent name. Press Cancel, put back the cassette on the reagent
tray and press Start button for more than two seconds. To rerun calibrations and controls, open the Selection tab ( Main menu >
■ If a new reagent lot number has been loaded, the pop-up message Order > Selection (tab) ). Select the position of the tube in the worklist and
displays "Lot number different for" and indicates the cassette unselect the tests that do not need to be rerun. Delete from the list calibrators
position and the reagent name. Press Cancel, delete the worklist, and controls that are valid.
run a new calibration and controls for the test before ordering the
worklist again.
■ If not enough reagent to perform the ordered tests, the instrument
displays the following pop-up message: "Reagent inventory may be If after rerunning calibrations and/or controls the error persists, please refer to
insufficient to process all test ordered, continue measurement?". the troubleshooting procedure of the user manual. See Maintenance and
Troubleshooting > Troubleshooting chapter.

The instrument starts measurement and the status "ANALYSING" is displayed.

Daily Guide 16
Ref: RAB275CEN
Workflow
Patient Samples

4.5. Patient Samples 4.5.1.1. To Register Samples by Position

Access: Main menu > Order > Selection (tab)

If necessary, register patient data in the Patient screen before sample registration ( Main
menu > Order > Patient (tab) ).

1. Select an available position in the worklist ( Main menu > Order > Selection (tab) ).
4.5.1. To Order Patient Samples
2. Select Normal in the Type dropdown list.
3. Select the cup type (Normal or Biocup) from the Cup dropdown list.
Access: Main menu > Order > Selection (tab) When a sample is registered, the instrument automatically gives a SID corresponding
to the sample position on the tray.
1. Open the main cover.
2. Open the Sample and Reagent Container Unit (SRCU) cover. 4. If using a SID, type the SID in the SID field (up to 12 digits).

3. If necessary remove all the tubes from the sample tray.

4. If necessary delete all the tubes from the worklist in the Selection screen: After saving the sample registration, the instrument automatically selects
a. Press Delete All. the next position on the tray and the SID is automatically incremented.
b. Press Save to validate the deletion.
A pop-up is displayed and allows you to confirm or to cancel the deletion.

5. If using a PID, select the corresponding patient in the PID field.

5. Register samples in the worklist according to your sample management method.
6. Select the sample category (Common, Serum, Urine, Plasma).
■ Sample position registration,
■ Sample registration with barcode, 7. If necessary select patient range.
■ Sample registration with host connection (LIS). 8. Choose tests to run for the selected sample by checking the corresponding tests.
9. Press Save to save sample registration.
10. Repeat the procedure for each sample to register.
15 positions are available on the sample tray. A 16th position is available in
the STAT unit. 11. If all the samples are registered in the worklist, press >> to check the worklist and
the corresponding sample position and ID.
12. Load the samples on the sample tray according to the worklist.

Daily Guide 17
Ref: RAB275CEN
Workflow
Patient Samples

■ Ensure that a sufficient sample volume without air bubble or foam is
placed on the instrument (refer to Workflow > Sample Materials chapter).
■ Caps must be removed from the sample cups as they may hit the
Sample and Reagent Container Unit (SRCU) cover.
■ Samples registered by this method stay in memory from run to run as long
as they are not run.
■ When you start the run, the instrument scans the tubes for barcode
identification and assigns the corresponding positions on the sample tray
to each tube.

4.5.1.2. To Register Samples with Barcode

New registration cannot be performed during analysis.
Access: Main menu > Order > Selection (tab)

If necessary, register patient data in the Patient screen before sample registration ( Main
menu > Order > Patient (tab) ).
4.5.1.3. To Register Sample with Host Connection (L.I.S)
1. Select the empty position in the Start Pos field (top of the dropdown list).
2. Select Normal in the Type dropdown list.
Access: Main menu > Order > Selection (tab)
3. Select the cup type Normal from the Cup dropdown list.
4. Type the barcode in the SID field. The host communication mode is set in the Host Connection screen ( Main menu >
5. If using a PID, select the corresponding patient in the PID field. Services > System Configuration > Host Connection (tab) ). Three communication
options are available:
6. Select the sample category (Common, Serum, Urine, Plasma).
■ Off Line
7. Select patient range.
■ On Line Batch
8. Choose tests to run for the selected sample by checking the corresponding tests. ■ On Line Query
9. Save sample registration.
The sample registered appears in the worklist below the STAT position 16 (E) at the 1. If the host communication mode is set to On Line Batch:
bottom of the list. a. If starting a day of work, press Acquire Orders from the Selection screen. The
next orders during the day are automatically acquired by the system.
The worklist registered on the laboratory information system is loaded or updated
10. Repeat the procedure for each sample to register.
on the instrument.
11. Load the samples on the sample tray.
b. Load the samples on the sample tray.
■ Ensure that a sufficient sample volume without air bubble or foam is
placed on the instrument (refer to Workflow > Sample Materials chapter). 2. If the host communication mode is set to On Line Query, load the samples on the
■ Caps must be removed from the sample cups as they may hit the sample tray.
Sample and Reagent Container Unit (SRCU) cover.

Daily Guide 18
Ref: RAB275CEN
Workflow
Patient Samples
When a sample tube is detected on board, the instrument automatically checks for
orders registered on the laboratory information system and loads orders concerning
the tube on the instrument.
■ Ensure that a sufficient sample volume without air bubble or foam is
placed on the instrument (refer to Workflow > Sample Materials chapter).
■ Caps must be removed from the sample cups as they may hit the Sample
and Reagent Container Unit (SRCU) cover.
4.5.1.4. To Start Sample Measurement
Before you start measurements, the following conditions must be fulfilled:
■ Enough new cuvettes must be loaded on the instrument.
■ Dust pod must allow to store cuvettes used during measurements.
■ Reagents, diluents and wash solutions are on board and enough for ordered
measurements.
■ Check that the water tank is full, if not fill it up with distilled water.
■ Check that the water tubing end stands in the bottom of the water tank.
■ Check that the waste tank is empty, if not empty it.
■ The cover of the Sample and Reagent Container Unit (SRCU) must be closed.
■ The instrument main cover must be closed.
■ The STAT unit must be closed.
Press Start button for more than two seconds.
If not enough cuvettes on board, the message "Shortage of cuvette or
disposal space for all orders. Continue the measurement?" is displayed.
Daily Guide
Ref: RAB275CEN
A pop-up is displayed: "Preparing for measurement start".
■ If a cassette has been removed or if a new reagent lot number has been
loaded since the last barcode scan, the following pop-up message is
displayed: "The reagents placed on the SRCU do not match with the
registered information. Start measurement?".
■ If a cassette has been removed, the pop-up message displays
"Cassette removed" and indicates the cassette position and the
reagent name. Press Cancel, put back the cassette on the reagent tray
and press Start button for more than two seconds.
■ If a new reagent lot number has been loaded, the pop-up message
displays "Lot number different for" and indicates the cassette position
and the reagent name. Press Cancel, delete the worklist, run a new
calibration and controls for the test before ordering the worklist again.
■ If not enough reagent to perform the ordered tests, the instrument displays
the following pop-up message: "Reagent inventory may be insufficient to
process all test ordered, continue measurement?".
The instrument starts measurement and the status "ANALYSING" is displayed.
The instrument displays the start time and the estimated finish time for measurements in
the main screen.
4.5.2. To Run Emergency Samples
Access: Main menu > Order > Selection (tab)
STAT position on the left-hand side of the instrument is dedicated to emergency samples.
19
Workflow
Patient Samples

For the STAT position, sample height within the tube must be higher than 12.6 As soon as the STAT button is available, you are able to run a new
mm. emergency sample.

1. Press the STAT button from the generic toolbar.

13. To run a new emergency sample, proceed as follows:

This action enables you to open the STAT unit. Open the STAT unit when the status
changes from "ANALYSING" to "WAITING".
2. Open the STAT unit by pushing slightly on the STAT cover on the left-hand side of
the instrument.
Before replacing the emergency sample, check the STAT sample status
from the main screen.
■ If this one is available (green), you can replace it immediately and
without condition.
■ If this one is still in progress (orange), you can replace it now but if an
automatic rerun is needed, it will not be performed!

3. Insert the emergency sample (sample tube or sample cup with adaptor) and push the
STAT unit back into the instrument.
4. Select position 16 from the Start Pos dropdown list and Normal from the Type a. Press the STAT button from the generic toolbar.
dropdown list in the Selection screen.
5. Select the cup type (Normal or Biocup) from the Cup dropdown list.
6. If using a SID, type the SID in the SID field (up to 12 digits).
7. If using a patient ID, select the corresponding patient in the PID field.
This action enables you to open the STAT unit.
8. Select the sample category (Common, Serum, Urine, Plasma).
9. If necessary, select the patient range. b. Select the Replace check box in the Selection screen.
10. Choose tests to run for the selected sample by checking the corresponding tests. c. Repeat steps from 2 to 12 to replace and to run the emergency sample.
11. Press Save to save emergency sample registration.
12. Press Start for more than two seconds to restart measurements.

No barcode reader is available for emergency sample. You must enter

information manually in the worklist.

The emergency sample requested is run in priority before any remaining samples
from the worklist.

Daily Guide 20
Ref: RAB275CEN
Workflow
Patient Samples

4.5.3. To Check Results 4.5.3.2. To Check or To Print Results from the Results Screen

Access: Main menu > Results

4.5.3.1. To Display Current Results

Access: Main menu > Run > Patient (tab)

Results are displayed in the Run screen as soon as the measurement is done.

1. Check the results from the run in the Patient tab from the Run screen.

Only on this screen, the instrument adds to the SID of each sample a prefix
corresponding to the sample type and the cup type (N for normal cups, n
for biocups, E for emergency samples and R for replicates).

2. If results are flagged, please refer to Workflow > Patient Sample > To Check Results
> To Manage Flagged Results chapter.

■ Results from the run are automatically transferred to the Results screen.
■ When a new run is started, results from the previous run are deleted from
1 = SID/PID dropdown list
the Run screen.
2 = SID/PID fields

3 = Date management area

4 = Output selection area

5 = Search button

6 = Results information area

1. Select SID or PID from the SID/PID dropdown list.

2. Type the SID or PID corresponding to the desired patient results.

Daily Guide 21
Ref: RAB275CEN
Workflow
End of Day

4.5.3.3. To Manage Flagged Results

In some cases, the SID is changed by the instrument:
■ For normal samples, the SID is not changed by the instrument. Type
the SID you registered for the sample.
■ For emergency samples, type the prefix 99000 before the registered SID.
■ For replicates, type the prefix 9400 before the registered SID.
■ Use "*" to select all the SID or PID.
3. Select the period or the date to display in the Date management area.
4. Select Monitor from the Result Output dropdown list.
5. Press Search to display the results on the screen.
Access: Main menu > Order > Selection (tab)
Samples which display an error flag are still registered in the worklist.
1. Check sample tubes.
2. If necessary, rerun the test:
a. Check that the samples to be rerun are in the worklist.
b. Select the tests to rerun for each sample.
c. If necessary delete samples which do not need to be rerun.
d. Press Start more than two seconds to start measurements.

If more than 2000 items are available for the specified search, a pop-up is
displayed: "Too many results meet search condition. Narrow the condition
and try again." Press OK and narrow the search specifications.

4.6. End of Day

6. Check results in the results information area.
7. If results need to be printed:
a. Select Condensed report from the Result Output dropdown list.
After a day of work, the user needs to remove the samples, to delete the worklist and to
b. Press Search more than one second to print the results report. A printer in ready perform the instrument cleaning with the daily maintenance procedures.
mode needs to be connected to the Pentra C200 (option). If a printer (option) is connected to the instrument, it can be switched off.

To print standard report, first select Normal from the Sample Type
dropdown list, then select Standard report from the Result Output 4.6.1. To Empty the Worklist
dropdown list.

Access: Main menu > Order > Selection (tab)

8. If results are flagged, please refer to Workflow > Patient Sample > To Check Results After a day of work, before performing the instrument cleaning, the worklist needs to be
> To Manage Flagged Results chapter. deleted and the samples removed from the Sample and Reagent Container Unit (SRCU).

Daily Guide 22
Ref: RAB275CEN
Workflow
End of Day

1. Check the worklist. 1. Open the main cover.

If samples are still registered in the worklist, check that they do not need to be run or 2. Open the Sample and Reagent Container Unit (SRCU) cover.
rerun.
3. Pour 2 mL of Etching solution from an Etching cassette (ABX Pentra Etching CP
2. If samples remain in the worklist and no test needs to be ordered for them: A11A01769) into a tube.
a. Press Delete All. 4. Place the Etching tube at position 14 in the sample tray.
b. Press Save to validate the deletion. 5. Close the SRCU cover.
A pop-up is displayed and allows you to confirm or to cancel the deletion. 6. Close the main cover.

3. Open the main cover and the SRCU cover.

4.6.2.2. To Perform the ISE Cleaning
4. Remove the tubes from the sample tray.

Access: Main menu > Order > Selection (tab)

At the end of the week, you must perform the weekly procedure described in
the Maintenance and Troubleshooting > Maintenance > Weekly Procedures
chapter of the user manual.
4.6.2. To Clean ISE with Etching (Option)
Follow this procedure every day if running more than 20 samples per day on
the ISE unit. If running less samples, ISE cleaning needs to be performed once
a week, at the end of the week.
1. Select position 14 from the Selection screen.
2. Select ISE Cleaning2 from the Type dropdown list.
3. Press Save to save the ISE cleaning registration.
4. Open the Sequence screen ( Main menu > Services > Customer Services >
Sequence (tab) ).
5. Press ISE Cleaning2.
6. Wait until the instrument is in "READY" status and remove the Etching tube from the
sample tray.
7. Delete the worklist:
a. Open the Selection screen ( Main menu > Order > Selection (tab) ).
b. Press Delete All.
c. Press Save to validate the deletion.
A pop-up is displayed and allows you to confirm or to cancel the deletion.

4.6.2.1. To Load Etching Solution on the Sample Tray

Always perform an ISE activation after ISE cleaning.

■ Disposable gloves must be worn by the operator.
■ Tools and consumables used during this procedure: Etching solution (ABX Pentra
Etching CP A11A01769).

Daily Guide 23
Ref: RAB275CEN
Workflow
End of Day

4.6.2.3. To Perform an ISE Activation

■ During the instrument operation, do not remove the waste liquid tube
under any condition.
Access: Main menu > Order > Selection (tab)
■ Waste must be handled according to your local/national regulations.

1. Open the main cover.

2. Open the Sample and Reagent Container Unit (SRCU) cover.
3. Place a serum or a plasma sample in an available position in the sample tray. 4. If the instrument is equipped with an ISE module (option), check ISE solutions before
a prolonged stand-by period within four days (over the week-end).
4. Select the sample tray position with the serum or the plasma tube. a. In Services > Customer Services > WorkHour (tab) check the remaining
5. Select the ISE Activation from the Type dropdown list. volume for Standard 1 and Reference solutions.
6. Press Save to register the ISE activation. b. If the volume is lower than 28 mL for Standard 1 solution or 10 mL for Reference
solution, replace the bottle with a new one.
7. Close the SRCU cover.
8. Close the main cover.
9. Open the Sequence screen ( Services > Customer Services > Sequence (tab) ).
10. Press ISE Activation. If the instrument is not used for more than four days, perform an extended
Wait until the end of the ISE activation: instrument status changes from shutdown with ISE. Please refer to your user manual, see Maintenance and
"ANALYSING" to "READY". Troubleshooting > Maintenance > Other Procedures > To Perform an
Extended Shutdown with ISE chapter.
11. Delete the worklist:
a. Open the Selection screen ( Main menu > Order > Selection (tab) ).
b. Press Delete All.
c. Press Save to validate the deletion.
A pop-up is displayed and allows you to confirm or to cancel the deletion.
4.6.4. To Clean Sample and Reagent Container Unit

4.6.3. To Check System Solutions
1. Check that the water tank is full, if not fill it up with distilled water.
2. Check that the water tubing end stands in the bottom of the water tank.
3. Check that the waste tank is empty, if not empty it.
According to your geographical location, the SRCU may have condensation and may
need to be wiped after every day of work.
To follow the SRCU cleaning procedure, please refer to the maintenance chapter of the
user manual. See Maintenance and Troubleshooting > Maintenance > Daily Procedures >
To Clean Sample and Reagent Container Unit chapter.

Daily Guide 24
Ref: RAB275CEN
Workflow
End of Day

4.6.5. To Clean the Probe with Deproteinizer

Access: Main menu > Inventory > Reagent Tray (tab)

A pop-up with several choices (Sleep, Power Off, Logout, Restart, Cancel) is
■ Disposable gloves must be worn by the operator. displayed.
■ Tools and consumables used during this procedure: Deproteinization solution (ABX
Pentra Deproteinizer CP A11A01754). 2. Press Sleep.
A pop-up is displayed to allow the probe wash configuration.
Every two weeks, perform tubing and tank decontamination before probe
cleaning. 3. Configure the Presoak Probe Wash area:
a. Press ON.
1. Check that a valid Deproteinizer cassette (ABX Pentra Deproteinizer CP b. Press S for special wash (W: water wash, D: DET.W wash).
A11A01754) is on board.
c. Press OK to start the probe wash.
2. If no Deproteinizer cassette is on board:
a. Load a Deproteinizer cassette on the reagent tray.
b. Open the Inventory screen. Instrument displays a pop-up to warn the user that the preparation of sleep operation is
processing and performs probe wash with the deproteinizer solution.
c. Perform a barcode scan.
The instrument switches to sleeping mode when the cleaning is finished. In sleeping
mode the green LED is blinking and the screen is black.
3. Enter in sleeping mode to start the probe cleaning with deproteinizer (see Workflow >
End of Day > Sleeping Mode > To Enter Sleeping Mode chapter).
The probe cleaning starts by entering in sleeping mode. It consists of a 10-minute
presoak of the probe plus a 10-minute presoak of the mixer paddle in the 4.6.6.2. To Perform a Manual Wake Up
deproteinizer solution dispensed in a cuvette.

1. Press the touch screen to wake the instrument up.

The main screen is displayed and the instrument status is "ANALYSING".

4.6.6. Sleeping Mode 2. Press the Shutdown button in the generic toolbar at the top of the screen.

4.6.6.1. To Enter Sleeping Mode

Access: Main screen
3. Press Sleep Cancel from the pop-up.
1. Press the Shutdown button in the generic toolbar at the top of the main screen.

Daily Guide 25
Ref: RAB275CEN
Workflow
To Stop in Emergency During Analysis

4. Press OK to confirm wake up from the pop-up: "Conduct the cancel of sleep ■ A pop-up with several choices (Power Off, Restart, Cancel) is displayed.
operation?".
The instrument performs an initialization.
2. Press Cancel.
The door is unlocked and can be opened by the user.

3. If you need to change or to add sample or reagent.

4.7. To Stop in Emergency During Analysis
1. Press two seconds on the emergency stop button.
a. Open the main cover.
b. Open the Sample and Reagent Container Unit (SRCU) cover.
c. If adding reagent or changing reagent position, perform a barcode scan from the
Inventory screen.
d. If adding sample or changing sample position, delete and register a new worklist
( Main menu > Order > Selection (tab)).

4. Close the SRCU cover.

5. Close the main cover.
6. Press Start.
A prime is automatically performed at the beginning.

1 = Green LED

2 = Red LED

3 = Emergency stop button

■ The analysis in process is stopped.

■ The status turns to "STOPPED".
■ The alarm icon turns to red and the alarm message "Detected stop request" is
displayed.

Daily Guide 26
Ref: RAB275CEN
Flags
Error Flags

5. Flags

Error flag Description Error Cause

When R1 reagent aspirating, the PT liquid level is
R1S R1 Reagent shortage not detected or a hit of the bottom is detected in
SRCU.
When R2 reagent aspirating, the PT liquid level is
5.1. Error Flags R2S R2 Reagent shortage not detected or a hit of the bottom is detected in
SRCU.
When wash solution aspirating, the PT liquid level
R1WS R1 Wash solution shortage is not detected or a hit of the bottom in DET.W. (At
R1 timing).
When the instrument meets problems during measurements, the concerned tests are
When wash solution aspirating, the PT liquid level
flagged with an error flag. The table below displays error flags with their description and
R2WS R2 Wash solution shortage is not detected or a hit of the bottom in DET.W. (At
causes. R2 timing).
When dilution aspirating, the PT liquid level is not
DS Dilution shortage
Error rank 1 detected or a hit of the bottom is detected in SRCU.
When wash solution aspirating, the RPT liquid level
Error flag Description Error Cause WS Wash solution shortage is not detected or a hit of the bottom is detected in
Not received ISE SRCU.
IE1 There is no response from ISE for request command.
measurement request The absorbance (in time-course) could not be
ABSE Absorbance error
Not received ISE The measurement result from ISE could not be measured.
IE2
measurement result data received. R1B No R1 reagent bottle R1 reagent bottle has not been registered.
The measurement result from ISE could not be The remaining volume of R1 reagent has reached
IE3 Omission of ISE measurement R1S Volume Zero in R1 reagent
printed out due to the busy state in electrodes. zero.
R2B No R2 reagent bottle R2 reagent bottle has not been registered.
Error rank 2 The remaining volume of R2 reagent has reached
R2S Volume Zero in R2 reagent
zero.
Error flag Description Error Cause R1 wash solution bottle for method-to-method
R1WB No R1 wash solution bottle
The liquid level of PT is not detected or a hit of the wash has not been registered.
SS Sample shortage
bottom is detected in SRCU. Volume Zero in R1 wash The remaining volume of R1 wash solution for
R1WS
Non detection of the liquid The liquid level of PT is not detected or a hit of the solution method-to-method wash has reached zero.
SI1
level of sample discharge bottom has been detected in IRU. R2wash solution bottle for method-to-method
R2WB No R2 wash solution bottle
wash has not been registered.

Daily Guide 27
Ref: RAB275CEN
Flags
Error Flags

Error flag Description Error Cause Error flag Description Error Cause
Volume Zero in R2 wash The remaining volume of R2 wash solution for IRU temperature extremely
R2WS TE2 IRU temperature > 37+2(°C).
solution method-to-method wash has reached zero. high
DB No dilution bottle The dilution bottle has not been registered. RCU temperature extremely
TE3 RCU temperature > 15°C.
The remaining volume of the dilution bottle has high
DS Volume Zero in dilution bottle
reached zero. RCU temperature extremely
TE4 RCU temperature < 2°C.
Wash solution bottle for PT wash has not been low
WB No wash solution bottle
registered.

WS
Volume Zero in wash solution The remaining volume of the wash solution bottle Error rank 4
bottle for PT wash has reached zero.
The reagent lot number used is not as previous lot Error flag Description Error Cause
LOT Lot inconsistency
recorded. EXP The reagent is no longer valid. An expired reagent was used in the measurement.
PT wash has been failed. Check the alarm to Invalid reagent stability was used with the
SPW Failure of PT wash (SPT) STB Reagent stability expired
determine the reason for its fail. measurement.
Method-to-method washing has failed prior to a The reason is that the measurement was taken by
R1W Failure of PT wash (RPT1) R1 operation.. Check the alarm to determine the CTO Calibration expired a method of which calibration has not been
reason for its fail. implemented within a setting term.
Method-to-method washing has failed, prior to a The reason is that the measurement was taken by
R2W Failure of PT wash (RPT2) R2 operation.. Check the alarm to determine the CXP Control expired a method of which QC measurement has not been
reason for its fail. implemented within a setting term.
DWPB No DET.W bottle for PT DET.W bottle is not placed beside PT unit. Q Control error Executed the measurement with control error.
Volume Zero in DET.W The remaining volume of DET.W for PT has been An error of concentrations of No calibration curve, or ISE calibration ever has
DWPS CA?
solution for PT zero. conversions. been executed after the startup.
DWMB No DET.W bottle for MIX DET.W bottle is not placed beside MIX unit.
The remaining count of MIX washing by DET.W Error rank 5
EUT Effective count Zero of DET.W
has been zero.
Cuvettes in the cuvette rack are insufficient for Error flag Description Error Cause
CS Cuvette shortage
measurement.
An error out of the range of The value is not between Calibrator’s minimum
DF Full cuvette in DP DP full up with used cuvettes. OVR
calibrator. and maximum.
EST Anomalous measurement Error sampling stop. Check the alarm for the reason.

Error rank 6
Error rank 3
Error flag Description Error Cause
Error flag Description Error Cause LIN Linearity Limit Error Linearity Limit Error.
TE1 IRU temperature extremely low IRU temperature < 37-2(°C). PRO Prozone Limit Error Prozone Limit Error.

Daily Guide 28
Ref: RAB275CEN
Flags
Error Code for ISE

Error flag Description Error Cause 5.2. Error Code for ISE
The flag "AB1" is displayed when all measuring
points exceed the absorbance limit except the first
AB1 Absorbance Limit1 Error
one. No result is displayed (*****) and a rerun with a
post-dilution is performed.
When an error occurs during ISE calibration, an error is displayed in the Result of ISE
The flag "AB2" is displayed when more than 1 Calibration screen. The table below displays the error codes with their description.
point and less than 5 points are within the
AB2 Absorbance Limit2 Error absorbance limit. The first result is then calculated The error code for ISE module is a four-digit code:
with those points and a rerun with post-dilution is
performed. ■ 0XXX: electrode errors
■ 1XXX: ISE module errors

Error rank 7
Electrode errors
Error flag Description Error Cause ■ The first digit is 0 (zero) for electrode errors.
Used reagent lot number in the specimen or ■ The second digit is the error code for Na electrode.
Using the different lot number ■ The third digit is the error code for K electrode.
CLT control measurement has been different from the
of R1 or R2
reagent in the calibration measurement. ■ The fourth digit is the error code for Cl electrode.
The measurement was taken by sample type of
each method that does not match the sample type. Error code Description
STM Sample type Inconsistent The concentration value was converted with a 1 2-point calibration: response alert.
calibration curve that does not match the sample
type. 2 2-point calibration: stability alert.
3 2-point calibration: reference potential alert.

Error rank 8 4 2-point calibration: sensitivity alert.

5 Normal concentration sample: calibration potential alert.
Error flag Description Error Cause 6 Normal concentration sample: sample potential response alert.
DUP Duplicate Limit Error Duplicate Limit Error. 7 Normal concentration sample: sample potential response alert +
calibration potential alert.

Error rank 9 8 High/Low concentration sample: no alert.

9 High/Low concentration sample: reference potential alert.
Error flag Description Error Cause A High/Low concentration sample: sample potential response alert.
SEN Sensitivity Limit Error Sensitivity Limit Error. B High/Low concentration sample: sample potential response alert +
The calibration has failed due to insufficient data calibration potential alert.
CAL Failure of calibration
points etc.
Example: 0600 is the electrode error "Normal concentration sample: sample potential
Error rank priority is ordered from 1 to 9: 1 > 2 > 3 > 4 > 5 > 6 > 7 > 8 > 9. response alert." on the Na electrode.

Daily Guide 29
Ref: RAB275CEN
Flags
Error Code for ISE

ISE module errors

Error code Alarm Description

1001 2075 The pumping speed adjustment is beyond the limit.
1021, 1022 2076 Cannot detect Standard I at LB1 sensor.
1023, 1024 2077 Incorrect supplying quantity of Standard I (LB1).
1025 2085 Incorrect supplying quantity of Standard I (LB2).
1026 2084 Cannot detect Standard I at LB2 sensor.
1031, 1032 2078 Cannot detect Standard II at LB1 sensor.
1033, 1034 2079 Incorrect supplying quantity of Standard II (LB1).
1035 2087 Incorrect supplying quantity of Standard II (LB2).
1036 2086 Cannot detect Standard II at LB2 sensor.
1041, 1042 2080 Cannot detect the sample at LB1 sensor.
1043, 1044 2081 Incorrect supplying quantity of the sample.(LB1)
1045 2089 Incorrect supplying quantity of the sample.(LB2)
1046 2088 Cannot detect the sample at LB2 sensor.
1091 2082 Detects abnormal condition at LB sensor check.
1101 2083 Hardware abnormal.
1996 1984 ISE is working.
1997 1978 ISE module is under unmeasurable state.
1998 1975 ISE communication failure.
1999 1980 - 1983 ISE Unit errors.

Daily Guide 30
Ref: RAB275CEN