Component to be Equipment/Meth Specifications of Equipments Chemicals Quantities of

analyzed od Chemicals

Fatty Acid Gas NaOH 98%

Composition Chromatograph
AOAC Methanol 99.9%
Official 2tin each 500g tin
Anhydrous NaS2O4
Method Cd
5L
3a- 63 Pyridine 99.9%
2.5L
Toluene

Chloroform 99.8%

Trahydrofuran 99.9%,

n-hexane

Hydrochloric acid

Heptadecanoate

MSTFA

Glycerin

Mono-olein

Di-olein
 Triolein

Ricinolein

1,2,4 Butanetriol 99%

Tricaprin 99%

FAME (100%) of:

 Oleate

 Palmitate

 Linoleate

 Stearate

 Linolinate

 Acetonitrile

 Acetone

 Commercial TAG
standard

Tocopherol analysis HPLC Hplc system consisting of a high pressure pump, a Alfa,beta,gama and sigma
(ISO 9936) sample injection device, column thermostat adjusted tocopherol and tocotrienol
to 25oC(optional), afluorescence detector with the standard(a blend of wheat germ
excitation waveleght set at 295nm and emission
and soya bean oil may be used
waveleght at 330nm, and a recording monitor
instead)
A UV detector may be used but it is not
 recommended
HPLC analytical column(two type are possible)
250mm x 4mm, packed with microparticulate diol
having a mean particle size of about 5micrometer
OR
250 x 4.6mm, packed with microparticulate silica
having mean diameter of about 5micometer
Uv spectrometer capable of absolute measurement
oof absorbance at precisely define wavelength,with
a 10mm path cell.
Rotary evaporator
Graduated cylinder, of 1000ml capacity
Volumetric pipettes, of 5ml, 10ml and 20ml
capacities
If tocotrienol std is not
available(palm oil may be used)
Tetrahydrofuran,filtered through
an hplc nylon filter (0.45microM)
n-heptane filtered through an
hplc nylon filter (0.45microM)
HPLC mobile phase(solvent that
has been approved to reach
chromatographic resolution of
peaks.(relative retention time of
tocopherol and tocotrienol)
Methanol

Vloumetric flasks, 50ml and 25ml capacities

Ultrasonic bath
Sterols(ISO 12228) GC Round bottomed flask KOH(0.5mol/l)
Reflux condenser
Internal standard solution, betulin,
Glass column, with PTFE stopper, sintered glass frit 1mg/ml solution in acetone
and reservoir for 100ml, length 25cm, internal
diameter 1.5cm Ethanol, of minimum purity
95%(v/v)
Rotary evaporator
Aluminium oxide, neutral, 0.063mm
Developing tank made of galss, with ground glass
to 0.2 mm
lid, with plate dimension 20 x20cm
Diethyl ether
Micro syringe or micropipette, to deliver
100microliter
Silica gel thin chromatography
Oven, maintained at 105oC plates of dimension 20 x 20cm
thickness of layer 0.25mm
Desiccator
 Developing solvent, hexane/diethyl
Reaction vials, of 0.3ml capacity, with screw caps ether 1/1 (v/v)
and PTFE-lined seals
Std soln for TLC, 1mg/ml
GC for capillary column with split injector, flame cholesterol and 5mg/ml betulin in
ionization detector and suitable recorder
acetone
Capillary column, made of fused silica or glasss,
Sprying agent, methanol
legth 25 m to 60 m, internal diameter 0.2mm to 0.25
mm.stationary phase SE-54; film thickness about
Silylating reagent
0.1micrometer.

Microsyringe for gas chromatography

Analytical balance
Standard Test Inductively- Spectrometer—Either a sequential or simultaneous Base Oil, U.S.P. white oil, or a
Method for Coupled Plasma spectrometer is suitable, if equipped with a quartz lubricating base oil that is free of
Determination of Atomic Emission ICP torch and r-f generator to form and sustain the analytes, with a viscosity of about
Additive Elements in ICP-OES plasma. 4 cSt at 100°C.
Lubricating Oils by
Inductively Coupled Analytical Balance, capable of weighing to 0.001 g Internal Standard, (Required)—An
Plasma Atomic or 0.0001 g, capacity of 150 g. oil-soluble internal standard
Emission element is required. The following
Spectrometry (This Peristaltic Pump, (Recommended)—A peristaltic internal standards were
test method covers pump is strongly recommended to provide a successfully used in the
the quantitative constant flow of solution. interlaboratory study on precision:
determination of Ag, Be, Cd, Co (most common),
barium, boron, The pumping speed must be in the range 0.5 to 3 La, Mn, Pb, Y.
calcium, mL/min.
copper, magnesium, Organometallic Standards—Multi-
phosphorus, sulfur The pump tubing must be able to withstand at least element standards, containing
and zinc in unused 6 h exposure to the dilution solvent. Fluoroelastomer known concentrations
lubricating oils and copolymer tubing is recommended. (approximately 0.1 mass %)
additive) of each element, can be prepared
Solvent Dispenser, (Optional)—A solvent dispenser from the individual metal
calibrated to deliver the required weight of diluent Concentrates.
can be advantageous. Ensure that solvent drip does
not affect accuracy. Dilution Solvent—Mixed xylenes,
o-xylene, and kerosene
Specimen Solution Containers, 30 to 120 mL, glass
or polyolefin vials or bottles, with screw caps.

Vortexer, (Optional)—Vortex the sample plus

diluents mixture until the sample is completely
dissolved.

Ultrasonic Homogenizer, Optional—A bath-type or

probe-type ultrasonic homogenizer can be used to
homogenizer the test specimen.