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Group –V : Received 200mg/ kg body weight On the 7th day, 2 ml of 1% solution of carrageenan
of CEE. dissolved in saline was injected directly into the
Group –VI : Received 50mg /kg body weight pouch to induce an inflammatory response. The rats
of CFM. were orally pre-treated with either vehicle or
Group –VII : Received 100mg /kg body weight CFE/CFM/BFM or indomethacin 2h prior to the
of CFM. injection of carrageenan. The second dose of
Group –VIII : Received 200mg/ kg body weight of treatment was repeated after 24 h of the first
CFM. treatment. 48 h after carrageenan injection, the rats
Group –IX : Received 50mg/ kg body weight were anesthetized with ether and the pouch was
of BFM. carefully opened by a small incision. The volume of
Group – X : Received l00mg/kg body weight exudates was collected and measured. An aliquot of
of BFM. the exudate was used for differential cell count
Group –XI : Received 200mg/kg body weight (neutrophils and monocytes) using a manual cell
of BFM. counter after staining with Wright's stain. The results
were expressed as the total number of neutrophils and
In-vivo pharmacological studies monocytes.
Experimental Animals
Wistar rats of either sex weighing 100–160 g was STATISTICAL ANALYSIS
used in the study and fed with standard laboratory Results of antiinflammatory activity were expressed
pellet diet; Provimi limited (India), provided water ad as Mean ± SD. Results were analyzed using one way
libitum and were maintained at 23–25◦C, 35 to 60% ANOVA. Differences were considered as statistically
humidity, and 12 h light/dark cycle. The rats were significant at p < 0.05 are compared to control.
acclimatized to the laboratory conditions for a period
of 7 days prior to experiment. The experimental RESULTS:
protocol (1468/PO/a/11/CPCSEA, June 8th 2011) was The data is shown in Table 1 and Figure 2 and 3. The
duly approved by institutional animal ethics CEE, CFM and BFM have shown dose dependent
committee (IAEC). Before the experiment, food was reduction in exudate volume significant (p < 0.05) as
withdrawn overnight but adequate water was given to compared to control group. 10 mg/kg body weight of
the rats. indomethacin showed significant (p < 0.05) result.
The BFM showed good activity at low dose than
Carrageenan Induced Air-Pouch Model CEE and CFM as compared to standard group. The
The rats were divided into eleven groups (n = 6). Air- activity was represented as follows: Indomethacin >
pouch was produced according to the method BFM > CFM > CEE.
described by Salvemini et al, 1996, briefly, rats were
anesthetized and air cavities were produced by The results of the study recommend that the CEE,
subcutaneous injection of 20 ml of sterile air into the CFM and BFM in dissimilar doses significantly
intra scapular area of the back (that is, 0 day). An restrained carrageenan provoked exudate volume,
additional 10 ml of air was injected into the cavity monocyte and neutrophil count in rats and exhibited
every 3rd day (3rd and 6th day) to keep the space open. significant anti-inflammatory action.
Table.1: Effect of CEE, BFM and BFM on exudate volume, neutrophil and monocyte count in Carrageenan
induced air-pouch inflammation
Neutrophils (X Monocytes
Treatment Dose Exudate volume
10cells) (X 10cells)