BT-503 Bioinformatics - I

Module 1:

 Different sub areas (Genomics, Proteomics etc) of bioinformatics- a general view

about application relating biological research and application to biotechnology.

 NCBI different modules:

 ORF,

 GenBank,

 Blast,

 OMIM,

 Taxonomy browser,

 PubMed.

Module 2:

 Vi editor,

 Shell Scripts,

 Perl program,

 How to operate EMBOSS.

Module 3:

 Sequence analysis:

 Introduction to sequence analysis,

 local and global alignment,

 pair wise and multiple alignment,

 sequence alignment algorithm:

 Dot matrix, Needleman and Wunsch algorithm,

 Smith-Waterman,

 Substitution Matrix

 (introduction: PAM, BLOSUM),

 BLAST,

 FASTA algorithms.

Module 4:

 Motif identification-
 Pfam,Prosite,
 Prediction of ORF,
 Promoter.
 A brief introduction of gene prediction,
 Bio-perl .