Beruflich Dokumente
Kultur Dokumente
BEGIN WITH
GOOD PREPARATION
2
DIGESTION OVERVIEW
The process of sample digestion is the chemical reaction of the HF is typically used in the digestion of silicates, rare-earth metal
sample with reagents (typically strong acids) in an effort to remove alloys, geologic samples, and occasionally for ceramics and
the sample matrix and leave analyte ions suspended in solution. polymers that will not respond to more typical digestion acids.
The sample and reagents are heated in an appropriate container to
The advantage of HF is that it has a vapor pressure similar to water,
speed the reaction. In many cases, the energy threshold requirements
so does not cause excessive pressure during digestion. HF also does
for reaction cannot be met by the temperatures reached using open-
not typically form molecular species that cause interferences during
vessel digestion. Closed-vessel microwave-assisted digestion creates
ICP-MS analysis.
a pressurized, high-temperature environment by containing the gases
produced during sample heating. The result is faster, more complete However, HF has several disadvantages. HF is very dangerous in
digestion than possible at atmospheric pressure. contact with skin or when the vapors are inhaled. A complete
understanding of HF hazards is required prior to use. When HF is
During digestion, there is always some vessel-to-vessel temperature
used for digestion, it must be neutralized or complexed prior to use
variation, the result of microwave field flux variabilities, differing
in any instrument sample introduction system that contains
microwave absorption rates among the samples, and chemical
glassware (see “Complexation of HF” in the Supplemental section).
reactions during digestion of the sample. The PerkinElmer Titan MPS
Additionally, HF will form compounds with Group I and II metals
Microwave Sample Preparation System is designed to minimize
which can precipitate as a fine cloudy haze. These precipitates can
these effects, and any remaining variation in vessel temperatures
be brought back into solution by complexing with boric acid in
should not affect digestion success.
a secondary digestion.
The Titan MPS system accurately measures the temperature of each
For digesting samples with HF, the following should be considered:
vessel, ensuring that the temperature of any individual vessel never
exceeds the target temperature in the method. This feature allows 1. What are the analytes of interest and would they require
setting high digestion temperatures while ensuring that the vessel HF to be freed from their matrix?
and sample remain under the method limits. 2. Is the sample introduction system on the analytical instrument
In general, during method development it is beneficial to design HF compatible?
the method to use the smallest sample weight that will deliver the 3. D
oes the lab have the capacity to perform two digestions
detection limits needed during subsequent instrumental analysis. for every sample (one for the sample, a second shorter one
Sample amounts larger than necessary can negatively affect the for complexing the HF)?
method by developing excessive pressure and increasing the
4. Is the infrastructure needed for the safe handling and response
potential for unwanted temperature variability, as well as shortening
to an HF exposure present?
consumable lifetimes.
The typical process of using HF for digestion is as follows:
The process of sample digestion commonly results in the evolution
of gas from the sample. Samples with high carbon content and 1. There is rarely a need for more than 1-2 mL of HF (49%)
metal oxides will evolve the most gas, and for samples of this type, per sample. The balance of the reagents (around 8-10 mL) will
the sample weights should be kept very small until the performance commonly be nitric acid, hydrochloric acid (HCl) acid or a blend
and parameters of the digestion are well known. In addition, most of the two.
digestion reactions are exothermic in nature; this “self-heating” can
2. H
F can be added first or last to the sample in the vessel.
rapidly cause the target temperature to be overrun, resulting in the
pressure within the vessel to exceed the desired limit. Once the 3. T he normal procedure to re-dissolve the fluoride salts is to add
heating, gas evolution, and reaction profile of the sample has been 6 mL saturated boric acid solution for each mL of HF, close the
determined, sample weights can be gradually increased, if needed. vessel, and heat to 190 °C for 10 minutes.
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DIGESTION OVERVIEW
13. Phosphoric acid is useful in the digestion of many types of time at the peak temperature were inadequate to completely digest
geologic or ore samples and also benefits from the ability to the sample. If additional time and temperature are unable to digest
dramatically reduce the pressure during digestion. Like sulfuric the sample, then it is likely that the acids used for digestion must be
acid, phosphoric acid has many potential interferences and changed. Please see the Digestion Tips section for insight on how to
will affect the viscosity of the final sample solution. Phosphoric attack the residue and enable effective cleaning of the vessel.
acid has extended rinse-out times and can form a residue on
ICP-OES and ICP-MS torches, injectors, as well as ICP-MS Cautions
cones. Phosphoric acid is expensive in its purest form. Microwave ovens are not spark-proof devices. As such, caution must
be taken when preparing samples for microwave digestion that are
Vessel and System Maintenance fine powders or that may be prone to spontaneous reaction or
To maintain top performance, vessels should be cleaned once ignition. If these samples remain on the sides of the vessel, inside
per week following the “Vessel Cleaning” method in the the vessel, or float on top of the reagents as a mass, there is the
Supplemental section. possibility of direct microwave interaction and generation of a
At a minimum, once per month the vessels should be re- spark. This spark initiates ignition of the remaining sample which
conditioned following the “Vessel Re-Conditioning” method can damage the vessel and/or the Titan MPS systems itself. While
in the Supplemental section. the Titan MPS is designed to capture this energy and reduce
danger to personnel and the lab environment, preventing an event
Over time, the gases generated during digestion can permeate the of this nature is important.
internal surface layer of the digestion vessels or liners. This is true
of all PTFE materials and does not indicate adsorption or permeation When handling fine powder or reactive samples, it is important that
of analytes. This gaseous permeation will generally be noticed as a the sample be completely rinsed to the bottom of the vessel and
“yellowing” of the vessel/liner or the acquisition of a slightly orange either well mixed with the reagents or completely submerged by the
tint to the vessel. As these gases accumulate, they reduce the reagents. Placing the sample into the vessel first and then using the
microwave transparency of the vessel itself and can cause the vessel reagents to wash down the walls of the vessel during addition is a
to be directly heated by the microwave energy applied. This can common solution. Optionally, a small amount of DI H2O can be used
to rinse the sample from the vessel walls and, normally, will not
result in uneven heating of samples during digestion and potentially
affect the digestion. Additionally, PerkinElmer offers PTFE weigh
cause damage to the vessel itself.
boats that, after weighing the sample, can be placed into the
Vessels and liners can be de-gassed by placing in a lab oven and vessel and remain there during digestion.
baking overnight at 200-220 °C. Optionally, the frequent
When trying to break up floating sample and swirling the vessel is
use of the “Vessel Re-Conditioning” method will help slow the
not effective, stirring of the sample into the reagents can be done
accumulation of gases and may even prevent the need for
using a long PTFE stir stick which is then rinsed off with a small
de-gassing of the vessels or liners.
amount of DI H2O.
Vessel Cleaning Between Digestions When heating samples in the microwave, any microwave energy not
During normal digestions, vessels or liners do not require cleaning absorbed by the samples or reagent will either be reflected back
between samples as the sample has been completely digested; to the magnetrons, resulting in less efficient operation, or will be
simply rinsing with DI H2O is enough to prep the vessels for the absorbed by a part of the microwave system not intended to be
next digestion. This remains true as long as the concentrations heated. For this reason, each published microwave method indicates
of elements in the sample remain similar and all digestions result in that the maximum power of the first step should be reduced when
total sample digestion. If analyzing samples where the concentration using fewer than the maximum number of vessels.
of the elements of interest is expected to differ by more than two
Additionally, very fast temperature ramps should be avoided as
orders of magnitude, it is advisable to run the “Vessel Cleaning”
a great deal of excess microwave energy will be generated in an
method in the Supplemental section.
effort to rapidly heat the sample. In all cases, a more moderate
If the previous digestion was not successful and material remains in temperature ramp is more efficient and will generate superior
the vessel, cleaning will be required to prepare the vessel for the digestion results.
next sample. There are typically two reasons for material to remain
after digestion. The first is that the peak temperature and length of
5
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A LOOK INSIDE
To ensure safe, accurate and reproducible digestions, the Titan MPS employs sophisticated Direct
Pressure Control™ (DPC)™ and Direct Temperature Control™ (DTC)™ technologies. With these
technologies, the Titan MPS is able to deliver outstanding reaction and digestion control.
Polarized
Light
Glass Ring
Optical
Pressure
Internal Detection
Pressure
IR Filter Technology
IR Temperature
Detection
6
TABLE OF CONTENTS
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TABLE OF CONTENTS
8
TABLE OF CONTENTS
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TABLE OF CONTENTS
10
TABLE OF CONTENTS
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TABLE OF CONTENTS
12
TABLE OF CONTENTS
SUPPLEMENTAL
• Complexation of HF........................................................................................................................................................... 210
• New Vessel Conditioning................................................................................................................................................... 211
• Vessel Cleaning.................................................................................................................................................................. 212
• Vessel Re-Conditioning...................................................................................................................................................... 213
13
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TABLE OF CONTENTS
14
ENVIRONMENTAL DIGESTION METHODS
Asphaltite
REAGENTS REAGENTS
0.100 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 35 5 5 90 1 160 60 5 10 80
2 200 35 3 35 100 2 220 80 3 30 100
3 50 35 1 20 0 3 50 80 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and
closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary
digestion (located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power at
the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
15
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ENVIRONMENTAL DIGESTION METHODS
Bark
REAGENTS REAGENTS
0.500 g 1.000 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 3 90 1 160 60 5 3 80
2 190 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to
the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power at
the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
16
ENVIRONMENTAL DIGESTION METHODS
Bauxite
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 2 90 1 160 60 5 2 80
2 210 30 3 30 100 2 230 60 3 20 100
3 50 30 1 20 0 3 50 60 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to
the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of
the number of vessels digested.
17
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ENVIRONMENTAL DIGESTION METHODS
Calcites
REAGENTS REAGENTS
0.100 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 210 35 3 40 100 2 230 80 5 30 100
3 50 35 1 20 0 3 50 80 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power at
the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
18
ENVIRONMENTAL DIGESTION METHODS
Cement
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 200 35 3 30 100 2 220 80 5 20 100
3 50 35 1 15 0 3 50 80 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested
19
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ENVIRONMENTAL DIGESTION METHODS
REAGENTS REAGENTS
0.050 g 0.150 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 3 80
2 200 35 3 25 100 2 220 80 3 20 100
3 50 35 1 15 0 3 50 80 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power at
the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
20
ENVIRONMENTAL DIGESTION METHODS
Dolomites
REAGENTS REAGENTS
0.100 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 200 35 3 40 100 2 230 80 5 30 100
3 50 35 1 15 0 3 50 80 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power at
the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
21
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ENVIRONMENTAL DIGESTION METHODS
Feldspar
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 200 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
22
ENVIRONMENTAL DIGESTION METHODS
Fluorite
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 200 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
23
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ENVIRONMENTAL DIGESTION METHODS
Garnet
This digestion is not recommended for the PerkinElmer Titan MPS System
Standard 75 mL Digestion Vessel High-Pressure 100 mL Digestion Vessel
REAGENTS REAGENTS
- HNO3 (70%) 10 mL
- HF (49%) 2 mL
- 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 - - - - - 1 170 60 5 5 80
2 - - - - - 2 230 60 3 45 100
3 - - - - - 3 50 60 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power at
the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the number
of vessels digested.
24
ENVIRONMENTAL DIGESTION METHODS
REAGENTS REAGENTS
0.500 g 1.000 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 3 90 1 160 60 5 3 80
2 180 30 3 25 100 2 200 60 3 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
25
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ENVIRONMENTAL DIGESTION METHODS
Iron Ores
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 3 90 1 170 60 5 3 80
2 220 30 5 35 100 2 240 60 5 20 100
3 50 30 1 20 0 3 50 60 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power at
the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the number
of vessels digested.
26
ENVIRONMENTAL DIGESTION METHODS
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 200 35 3 35 100 2 220 80 3 20 100
3 50 35 1 15 0 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
27
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ENVIRONMENTAL DIGESTION METHODS
Ironstone
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 3 90 1 170 60 5 3 80
2 200 35 3 35 100 2 220 80 3 20 100
3 50 35 1 15 0 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
28
ENVIRONMENTAL DIGESTION METHODS
Kaolinite
REAGENTS REAGENTS
0.100 g 0.200 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 3 90 1 170 60 5 3 80
2 220 30 5 35 100 2 240 60 5 20 100
3 50 30 1 15 0 3 50 60 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
29
www.perkinelmer.com/titanmps
ENVIRONMENTAL DIGESTION METHODS
Leather
REAGENTS REAGENTS
0.100 g 0.200 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 15 100 2 200 60 3 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to
the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
30
ENVIRONMENTAL DIGESTION METHODS
Limestone
REAGENTS REAGENTS
0.050 g 0.150 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 3 80
2 200 35 3 30 100 2 220 80 5 20 100
3 50 35 1 15 0 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
31
www.perkinelmer.com/titanmps
ENVIRONMENTAL DIGESTION METHODS
Phosphate Rocks
REAGENTS REAGENTS
0.200 g 0.400 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 3 90 1 170 60 5 3 80
2 200 30 3 35 100 2 220 60 3 20 100
3 50 30 1 15 0 3 50 60 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
32
ENVIRONMENTAL DIGESTION METHODS
Platinum Ores
REAGENTS REAGENTS
0.050 g 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 3 90 1 170 60 5 3 80
2 210 30 3 45 100 2 230 60 5 30 100
3 50 30 1 20 0 3 50 60 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
33
www.perkinelmer.com/titanmps
ENVIRONMENTAL DIGESTION METHODS
Refinery Ash
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 200 35 3 35 100 2 230 80 5 20 100
3 50 35 1 15 0 3 50 80 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
34
ENVIRONMENTAL DIGESTION METHODS
This digestion is not recommended for the PerkinElmer Titan MPS System
Standard 75 mL Digestion Vessel High-Pressure 100 mL Digestion Vessel
REAGENTS REAGENTS
- H2SO4 (98%) 7 mL
- HNO3 (70%) 5 mL
HF (49%) 1 mL
- 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 - - - - - 1 160 60 5 10 80
2 - - - - - 2 190 80 5 10 100
3 - - - - - 3 240 80 5 30 100
4 - - - - - 4 50 80 1 45 0
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
35
www.perkinelmer.com/titanmps
ENVIRONMENTAL DIGESTION METHODS
Sediment
REAGENTS REAGENTS
0.500 g 1.000 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
36
ENVIRONMENTAL DIGESTION METHODS
REAGENTS REAGENTS
0.500 g 1.000 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
37
www.perkinelmer.com/titanmps
ENVIRONMENTAL DIGESTION METHODS
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
38
ENVIRONMENTAL DIGESTION METHODS
Shredded Paper
REAGENTS REAGENTS
0.500 g 1.000 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 150 30 5 3 90 1 160 60 5 3 80
2 180 30 2 5 100 2 180 60 2 5 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
39
www.perkinelmer.com/titanmps
ENVIRONMENTAL DIGESTION METHODS
Slag
REAGENTS REAGENTS
0.100 g 0.200 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 200 35 3 40 100 2 220 80 5 30 100
3 50 35 1 15 0 3 50 80 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
40
ENVIRONMENTAL DIGESTION METHODS
Soil
REAGENTS REAGENTS
0.500 g 1.000 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 180 35 2 30 100 2 210 80 5 15 100
3 50 35 1 15 0 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
41
www.perkinelmer.com/titanmps
ENVIRONMENTAL DIGESTION METHODS
REAGENTS REAGENTS
1.000 g 1.000 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
42
ENVIRONMENTAL DIGESTION METHODS
Tar
REAGENTS REAGENTS
0.150 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 8 10 90 1 170 60 8 10 80
2 200 30 3 30 100 2 220 60 3 20 100
3 50 30 1 10 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
43
www.perkinelmer.com/titanmps
ENVIRONMENTAL DIGESTION METHODS
REAGENTS REAGENTS
45 mL 45 mL
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
44
ENVIRONMENTAL DIGESTION METHODS
REAGENTS REAGENTS
0.250 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
45
www.perkinelmer.com/titanmps
ENVIRONMENTAL DIGESTION METHODS
REAGENTS REAGENTS
0.250 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
46
ENVIRONMENTAL DIGESTION METHODS
REAGENTS REAGENTS
20 mL 20 mL
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 200 30 3 10 100 2 200 60 3 10 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
47
www.perkinelmer.com/titanmps
ENVIRONMENTAL DIGESTION METHODS
REAGENTS REAGENTS
20 mL 20 mL
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 145 30 5 5 90 1 145 60 5 5 80
2 180 30 3 10 100 2 180 60 3 10 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power at
the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
48
ENVIRONMENTAL DIGESTION METHODS
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 10 90 1 170 60 5 10 80
2 200 30 5 25 100 2 220 60 5 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample
to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing
the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to
the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
49
www.perkinelmer.com/titanmps
TABLE OF CONTENTS
50
TABLE OF CONTENTS
51
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FOOD AND BEVERAGE DIGESTION METHODS
Algae (Paste)
REAGENTS REAGENTS
0.500 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 25 100 2 210 60 5 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
52
FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.500 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 25 100 2 210 60 5 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
53
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FOOD AND BEVERAGE DIGESTION METHODS
Agar
REAGENTS REAGENTS
0.250 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
54
FOOD AND BEVERAGE DIGESTION METHODS
Animal Feed
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
2 200 30 5 30 100 2 220 60 5 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
55
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FOOD AND BEVERAGE DIGESTION METHODS
Beer
REAGENTS REAGENTS
2 mL 4 mL
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
56
FOOD AND BEVERAGE DIGESTION METHODS
Bran
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 5 25 100 2 200 60 5 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
57
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FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
58
FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.500 g 1.00 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 5 20 100 2 200 60 5 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
59
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FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.500 g 1.00 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
60
FOOD AND BEVERAGE DIGESTION METHODS
Citrus
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 25 100 2 210 60 3 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
61
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FOOD AND BEVERAGE DIGESTION METHODS
Cocoa / Cacao
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
62
FOOD AND BEVERAGE DIGESTION METHODS
Coconut Oil
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 150 30 5 5 90 1 150 60 5 5 80
2 190 30 5 20 100 2 210 60 5 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
63
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FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
64
FOOD AND BEVERAGE DIGESTION METHODS
Coffee (Beverage)
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 5 20 100 2 210 60 5 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
65
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FOOD AND BEVERAGE DIGESTION METHODS
Corn Gluten
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 150 30 5 5 90 1 150 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
66
FOOD AND BEVERAGE DIGESTION METHODS
Corn Meal
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
67
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FOOD AND BEVERAGE DIGESTION METHODS
Corn Oil
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 150 30 5 5 90 1 150 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of
the number of vessels digested.
68
FOOD AND BEVERAGE DIGESTION METHODS
Corn Syrup
REAGENTS REAGENTS
0.200 g 0.400 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 150 30 5 10 90 1 150 60 5 10 80
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
69
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FOOD AND BEVERAGE DIGESTION METHODS
Crab Paste
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 150 30 5 10 90 1 150 60 5 10 80
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of
the number of vessels digested.
70
FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
2 mL 4 mL
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
71
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FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
1 mL 2 mL
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 150 30 5 5 90 1 150 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of
the number of vessels digested.
72
FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.500 mL 1 mL
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 150 30 5 5 90 1 150 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
73
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FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.250 mL 0.750 mL
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 150 30 5 5 90 1 150 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
74
FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 150 30 5 10 90 1 150 60 5 10 80
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
75
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FOOD AND BEVERAGE DIGESTION METHODS
Fishmeal
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of
the number of vessels digested.
76
FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.300 g 0.800 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
77
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FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.200 g 0.400 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
78
FOOD AND BEVERAGE DIGESTION METHODS
Fruit (Dried)
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
79
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FOOD AND BEVERAGE DIGESTION METHODS
Fruit (Fresh)
REAGENTS REAGENTS
0.500 g 1.00 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
80
FOOD AND BEVERAGE DIGESTION METHODS
Fruit Juice
REAGENTS REAGENTS
2 mL 4 mL
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
81
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FOOD AND BEVERAGE DIGESTION METHODS
Herbs
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
82
FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
83
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FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
84
FOOD AND BEVERAGE DIGESTION METHODS
Meat
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
85
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FOOD AND BEVERAGE DIGESTION METHODS
Milk (Fresh)
REAGENTS REAGENTS
3.00 g 5.00 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
86
FOOD AND BEVERAGE DIGESTION METHODS
Milk (Powder)
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
87
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FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.400 g 0.800 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary
digestion (located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
88
FOOD AND BEVERAGE DIGESTION METHODS
Olive Cake
REAGENTS REAGENTS
0.400 g 0.800 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 80 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary
digestion (located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
89
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FOOD AND BEVERAGE DIGESTION METHODS
Olive Leaves
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
90
FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 10 90 1 170 60 5 10 80
3 50 30 1 15 0 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
91
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FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.400 g 0.800 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
92
FOOD AND BEVERAGE DIGESTION METHODS
Rice
REAGENTS REAGENTS
0.500 g 1.000 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
93
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FOOD AND BEVERAGE DIGESTION METHODS
Soy Lechithin
REAGENTS REAGENTS
0.250 g 0.700 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
94
FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
95
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FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
96
FOOD AND BEVERAGE DIGESTION METHODS
Sugar Beet
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
97
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FOOD AND BEVERAGE DIGESTION METHODS
Sugar Cane
REAGENTS REAGENTS
0.200 g 0.400 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 10 90 1 170 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
98
FOOD AND BEVERAGE DIGESTION METHODS
Tea
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
99
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FOOD AND BEVERAGE DIGESTION METHODS
Tomato Leaves
REAGENTS REAGENTS
0.500 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
100
FOOD AND BEVERAGE DIGESTION METHODS
Vegetable Oil
REAGENTS REAGENTS
0.300 g 0.700 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 15 90 1 160 60 5 10 80
2 190 30 3 20 100 2 210 60 3 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
101
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FOOD AND BEVERAGE DIGESTION METHODS
Wax (Edible)
REAGENTS REAGENTS
0.400 g 0.800 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
102
FOOD AND BEVERAGE DIGESTION METHODS
REAGENTS REAGENTS
0.800 g 1.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
103
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FOOD AND BEVERAGE DIGESTION METHODS
Whey Powder
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the
sample to the bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place
and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm
to the touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
104
FOOD AND BEVERAGE DIGESTION METHODS
Xanthum Gum
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
105
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TABLE OF CONTENTS
106
FORENSIC DIGESTION METHODS
Animal Fat
REAGENTS REAGENTS
0.200 g 0.400 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
2 190 30 3 20 100 2 210 60 5 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
107
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FORENSIC DIGESTION METHODS
Blood
REAGENTS REAGENTS
2.00 g 2.00 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 15 100 2 210 60 3 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
108
FORENSIC DIGESTION METHODS
Bone
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
109
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FORENSIC DIGESTION METHODS
Hair
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 20 100 2 210 60 3 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
110
FORENSIC DIGESTION METHODS
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
2 190 30 3 20 100 2 210 60 3 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
111
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FORENSIC DIGESTION METHODS
REAGENTS REAGENTS
0.600 g 1.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
2 190 30 3 20 100 2 210 60 3 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
112
TABLE OF CONTENTS
113
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TABLE OF CONTENTS
114
INDUSTRIAL DIGESTION METHODS
Activated Carbon
This digestion is not recommended for the PerkinElmer Titan MPS System
Standard 75 mL Digestion Vessel High-Pressure 100 mL Digestion Vessel
REAGENTS REAGENTS
- HNO3 (70%) 8 mL
H2SO4 (95%) 4 mL
-
- 0.050 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 - - - - - 1 170 60 5 20 80
2 - - - - - 2 220 80 7 40 100
3 - - - - - 3 50 80 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
115
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INDUSTRIAL DIGESTION METHODS
Alloy Scrap
REAGENTS REAGENTS
0.050 g 0.200 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 10 90 1 170 60 5 10 80
3 50 30 1 15 0 3 50 60 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
116
INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.050 g 0.200 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 15 90 1 170 60 5 20 80
3 50 35 1 15 0 3 50 80 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
117
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INDUSTRIAL DIGESTION METHODS
Aluminum
REAGENTS REAGENTS
0.150 g 0.400 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
118
INDUSTRIAL DIGESTION METHODS
Bakelite
REAGENTS REAGENTS
0.500 g 1.000 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 15 90 1 160 60 5 15 80
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
119
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INDUSTRIAL DIGESTION METHODS
Boron Carbide
REAGENTS REAGENTS
0.050 g 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 10 90 1 170 60 5 10 80
3 50 30 1 15 0 3 50 60 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
120
INDUSTRIAL DIGESTION METHODS
Brass
REAGENTS REAGENTS
0.100 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
121
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INDUSTRIAL DIGESTION METHODS
Bronze
REAGENTS REAGENTS
0.100 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
122
INDUSTRIAL DIGESTION METHODS
Calcined Coke
REAGENTS REAGENTS
0.050 g 0.200 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 10 90 1 170 60 5 10 80
3 50 30 1 15 0 3 50 80 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
123
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INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.050 g 0.150 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 10 90 1 170 60 5 5 80
3 50 35 1 15 0 3 50 80 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
124
INDUSTRIAL DIGESTION METHODS
Cigar / Cigarettes
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
125
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INDUSTRIAL DIGESTION METHODS
Coal
REAGENTS REAGENTS
0.050 g 0.200 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 170 60 5 10 80
3 50 35 1 15 0 3 50 80 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
126
INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
127
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INDUSTRIAL DIGESTION METHODS
Cobalt Tungstate
This digestion is not recommended for the PerkinElmer Titan MPS System
Standard 75 mL Digestion Vessel High-Pressure 100 mL Digestion Vessel
REAGENTS REAGENTS
- HNO3 (70%) 10 mL
- HF (49%) 5 mL
- 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 - - - - - 1 180 60 5 10 80
2 - - - - - 2 230 80 5 60 100
3 - - - - - 3 50 80 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
128
INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
129
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INDUSTRIAL DIGESTION METHODS
Crude Oil
REAGENTS REAGENTS
0.100 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 150 30 5 10 90 1 150 60 5 10 80
3 50 35 1 15 0 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
130
INDUSTRIAL DIGESTION METHODS
Diesel
This digestion is not recommended for the PerkinElmer Titan MPS System
Standard75 mL Digestion Vessel High-Pressure 100 mL Digestion Vessel
REAGENTS REAGENTS
- HNO3 (70%) 10 mL
- 0.050 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 - - - - - 1 150 60 5 15 80
2 - - - - - 2 200 80 7 15 100
3 - - - - - 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
131
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INDUSTRIAL DIGESTION METHODS
Electronic Chips
REAGENTS REAGENTS
0.250 g 0.600 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
2 190 35 3 35 100 2 210 80 5 25 100
3 50 35 1 15 0 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
132
INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.150 g 0.400 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
2 190 35 3 20 100 2 210 80 3 15 100
3 50 35 1 15 0 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
133
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INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.150 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
2 180 35 3 15 100 2 190 60 3 15 100
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
134
INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.400 g 0.800 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 200 30 3 40 100 2 220 60 3 35 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
135
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INDUSTRIAL DIGESTION METHODS
Inks
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 200 30 3 30 100 2 220 60 3 25 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
136
INDUSTRIAL DIGESTION METHODS
Indium-Tin Oxides
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 10 90 1 170 60 5 10 80
2 190 30 3 25 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
137
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INDUSTRIAL DIGESTION METHODS
Ionomer
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 190 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
138
INDUSTRIAL DIGESTION METHODS
Lanthanum Boride
REAGENTS REAGENTS
0.050 g 0.200 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 210 30 3 25 100 2 230 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
139
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INDUSTRIAL DIGESTION METHODS
This digestion is not recommended for the PerkinElmer Titan MPS System
Standard 75 mL Digestion Vessel High-Pressure 100 mL Digestion Vessel
REAGENTS REAGENTS
- HNO3 (70%) 10 mL
- HF (49%) 5 mL
- 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 - - - - - 1 170 60 5 5 80
2 - - - - - 2 230 60 5 45 100
3 - - - - - 3 50 60 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
140
INDUSTRIAL DIGESTION METHODS
Lithium Titanates
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 40 100 2 210 60 3 35 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
141
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INDUSTRIAL DIGESTION METHODS
Magnesium Oxide
REAGENTS REAGENTS
0.400 g 0.800 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 45 100 2 210 60 3 30 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
142
INDUSTRIAL DIGESTION METHODS
Melamine
This digestion is not recommended for the PerkinElmer Titan MPS System
Standard 75 mL Digestion Vessel High-Pressure 100 mL Digestion Vessel
REAGENTS REAGENTS
- HNO3 (70%) 5 mL
H3PO4 (86%) 5 mL
-
H2O2 (30%) 1 mL
-
- 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 - - - - - 1 170 60 5 10 80
2 - - - - - 2 230 80 5 45 100
3 - - - - - 3 50 80 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
143
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INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 190 30 3 45 100 2 210 60 3 35 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
144
INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 190 30 3 45 100 2 210 60 3 35 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
145
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INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.050 g 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
3 50 30 1 15 0 3 50 60 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
146
INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 210 30 3 45 100 2 230 60 3 35 100
3 50 30 1 15 0 3 50 60 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
147
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INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 200 30 3 50 100 2 220 60 3 40 100
3 50 30 1 15 0 3 50 60 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
148
INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 190 30 3 45 100 2 210 60 3 35 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
149
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INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.050 g 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 190 30 3 45 100 2 210 60 3 35 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
150
INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.050 g 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 10 10 90 1 170 60 10 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
151
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INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.050 g 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 10 10 90 1 170 60 10 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
152
INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
153
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INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.100 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
3 50 30 1 15 0 3 50 80 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
154
INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.050 g 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
3 50 30 1 15 0 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
155
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INDUSTRIAL DIGESTION METHODS
Molybdenum
REAGENTS REAGENTS
0.500 g 1.000 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
156
INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 15 90 1 160 60 5 15 80
3 50 30 1 15 0 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
157
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INDUSTRIAL DIGESTION METHODS
Niobium Disulfide
REAGENTS REAGENTS
0.050 g 0.200 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
158
INDUSTRIAL DIGESTION METHODS
Nylon
REAGENTS REAGENTS
0.150 g 0.400 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
159
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INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.300 g 0.750 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
160
INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.100 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
2 200 35 5 50 100 2 220 60 5 40 100
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
161
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INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.100 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
162
INDUSTRIAL DIGESTION METHODS
Polycarbonate
REAGENTS REAGENTS
0.100 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 190 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
163
www.perkinelmer.com/titanmps
INDUSTRIAL DIGESTION METHODS
Polyester
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
164
INDUSTRIAL DIGESTION METHODS
Polyetheretherketone (PEEK)
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
165
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INDUSTRIAL DIGESTION METHODS
Polyethylene Terephthalate
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
166
INDUSTRIAL DIGESTION METHODS
Polypropylene
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 45 100 2 210 60 3 35 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
167
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INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.200 g 0.400 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 15 90 1 170 60 5 15 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
168
INDUSTRIAL DIGESTION METHODS
Refractory Material
This digestion is not recommended for the PerkinElmer Titan MPS System
Standard 75 mL Digestion Vessel High-Pressure 100 mL Digestion Vessel
REAGENTS REAGENTS
- H3PO4 (85%) 5 mL
- H2SO4 (95%) 5 mL
- HF (49%) 2 mL
- HNO3 (70%) 1 mL
- 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 - - - - - 1 170 60 5 10 80
2 - - - - - 2 240 80 5 35 100
3 - - - - - 3 50 80 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
169
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INDUSTRIAL DIGESTION METHODS
Rhodium
REAGENTS REAGENTS
0.050 g 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 15 90 1 170 60 5 15 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
170
INDUSTRIAL DIGESTION METHODS
Selenium Powder
REAGENTS REAGENTS
0.150 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 200 30 3 30 100 2 220 60 3 25 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
171
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INDUSTRIAL DIGESTION METHODS
Silicon
REAGENTS REAGENTS
0.300 g 0.600 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 200 30 3 45 100 2 220 60 3 35 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
172
INDUSTRIAL DIGESTION METHODS
This digestion is not recommended for the PerkinElmer Titan MPS System
Standard 75 mL Digestion Vessel High-Pressure 100 mL Digestion Vessel
REAGENTS REAGENTS
- H3PO4 (85%) 6 mL
- HCI (35-37%) 4 mL
- HNO3 (70%) 2 mL
- HF (49%) 1 mL
- 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 - - - - - 1 170 60 5 10 80
2 - - - - - 2 240 80 5 35 100
3 - - - - - 3 50 80 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
173
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INDUSTRIAL DIGESTION METHODS
Silicon Oil
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
2 200 35 3 25 100 2 220 60 3 20 100
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
174
INDUSTRIAL DIGESTION METHODS
Slag (Furnace)
REAGENTS REAGENTS
0.100 g 0.200 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 190 35 3 45 100 2 210 80 3 35 100
3 50 35 1 15 0 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
175
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INDUSTRIAL DIGESTION METHODS
Solder
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
176
INDUSTRIAL DIGESTION METHODS
Sulfur (Elemental)
REAGENTS REAGENTS
0.150 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 30 1 30 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
177
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INDUSTRIAL DIGESTION METHODS
REAGENTS REAGENTS
0.200 g 0.400 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
178
INDUSTRIAL DIGESTION METHODS
Titanium Carbide
REAGENTS REAGENTS
0.050 g 0.200 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 10 90 1 160 60 5 10 80
3 50 35 1 15 0 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
179
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INDUSTRIAL DIGESTION METHODS
Titanium Diboride
This digestion is not recommended for the PerkinElmer Titan MPS System
Standard 75 mL Digestion Vessel High-Pressure 100 mL Digestion Vessel
REAGENTS REAGENTS
- HNO3 (70%) 10 mL
- HCI (35-37%) 4 mL
- HF (49%) 4 mL
- 0.100 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 - - - - - 1 170 60 5 5 80
2 - - - - - 2 220 80 3 40 100
3 - - - - - 3 50 80 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
180
INDUSTRIAL DIGESTION METHODS
Titanium Disulfide
This digestion is not recommended for the PerkinElmer Titan MPS System
Standard 75 mL Digestion Vessel High-Pressure 100 mL Digestion Vessel
REAGENTS REAGENTS
- HNO3 (70%) 5 mL
- HF (49%) 5 mL
- 0.200 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 - - - - - 1 170 60 5 5 80
2 - - - - - 2 230 80 3 30 100
3 - - - - - 3 50 80 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
181
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INDUSTRIAL DIGESTION METHODS
Tungsten
REAGENTS REAGENTS
0.100 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
3 50 30 1 25 0 3 50 80 1 45 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
182
INDUSTRIAL DIGESTION METHODS
Tungsten Carbide
REAGENTS REAGENTS
0.100 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 200 35 3 45 100 2 210 60 3 35 100
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
183
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INDUSTRIAL DIGESTION METHODS
Titanium Oxide
REAGENTS REAGENTS
HF (49%) 7 mL HF (49%) 10 mL
HNO3 (70%) 3 mL HNO3 (70%) 4 mL
0.150 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 35 3 30 100 2 210 60 3 20 100
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
184
INDUSTRIAL DIGESTION METHODS
Zeolites
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
3 50 35 1 15 0 3 50 80 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
185
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INDUSTRIAL DIGESTION METHODS
Zinc Oxide
REAGENTS REAGENTS
0.400 g 0.800 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 210 30 3 40 100 2 230 60 3 30 100
3 50 30 1 20 0 3 50 60 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
186
TABLE OF CONTENTS
187
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PERSONAL CARE PRODUCT DIGESTION METHODS
Body Lotion
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
188
PERSONAL CARE PRODUCT DIGESTION METHODS
Face Cream
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
189
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PERSONAL CARE PRODUCT DIGESTION METHODS
Lipstick
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
190
PERSONAL CARE PRODUCT DIGESTION METHODS
Shampoo
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
191
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PERSONAL CARE PRODUCT DIGESTION METHODS
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
192
PERSONAL CARE PRODUCT DIGESTION METHODS
Sunscreen
REAGENTS REAGENTS
0.150 g 0.350 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 35 5 25 100 2 220 70 5 15 100
3 50 35 1 15 0 3 50 70 1 40 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
193
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PERSONAL CARE PRODUCT DIGESTION METHODS
Talcum Powder
REAGENTS REAGENTS
0.100 g 0.300 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 200 35 5 35 100 2 210 70 5 25 100
3 50 35 1 15 0 3 50 70 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
194
PERSONAL CARE PRODUCT DIGESTION METHODS
Toothpaste
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
When using HF, if the sample is to be analyzed in an instrument containing glassware, the “Complexation of HF” secondary digestion
(located in the Supplemental section) must be performed.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
195
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TABLE OF CONTENTS
196
PHARMACEUTICAL AND NUTRACEUTICAL DIGESTION METHODS
REAGENTS REAGENTS
0.200 g 0.400 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 35 3 25 100 2 200 60 3 25 100
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
197
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PHARMACEUTICAL AND NUTRACEUTICAL DIGESTION METHODS
REAGENTS REAGENTS
0.200 g 0.400 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 35 3 25 100 2 200 60 3 25 100
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
198
PHARMACEUTICAL AND NUTRACEUTICAL DIGESTION METHODS
Cough Syrup
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 150 30 5 10 90 1 150 60 5 10 80
2 190 35 3 20 100 2 200 60 3 20 100
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
199
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PHARMACEUTICAL AND NUTRACEUTICAL DIGESTION METHODS
Ginseng
REAGENTS REAGENTS
0.500 g 1.000 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 190 30 3 20 100 2 200 60 3 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
200
PHARMACEUTICAL AND NUTRACEUTICAL DIGESTION METHODS
REAGENTS REAGENTS
0.200 g 0.400 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 140 30 5 3 90 1 140 60 5 3 80
2 190 30 3 15 100 2 190 60 3 15 100
3 50 30 1 15 0 3 50 60 1 25 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
If the sample is not totally dissolved when digestion is complete, add 2 mL of HCl (35-37%) to the reagents.
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
201
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PHARMACEUTICAL AND NUTRACEUTICAL DIGESTION METHODS
REAGENTS REAGENTS
0.400 g 0.800 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 190 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
If silicate material remains and complete digestion is desired, add 2 mL of HF (49%) to the reagents.
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
202
PHARMACEUTICAL AND NUTRACEUTICAL DIGESTION METHODS
REAGENTS REAGENTS
0.200 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 200 35 3 30 100 2 220 60 3 20 100
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
203
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PHARMACEUTICAL AND NUTRACEUTICAL DIGESTION METHODS
REAGENTS REAGENTS
0.100 g 0.250 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 190 30 2 10 100 2 190 60 2 10 100
3 210 30 2 15 100 3 210 60 2 15 100
4 50 30 1 20 0 4 50 60 1 30 0
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
204
PHARMACEUTICAL AND NUTRACEUTICAL DIGESTION METHODS
REAGENTS REAGENTS
0.400 g 0.800 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 200 30 3 45 100 2 220 60 3 30 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
205
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PHARMACEUTICAL AND NUTRACEUTICAL DIGESTION METHODS
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 190 30 3 30 100 2 210 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
If silicate material remains and complete digestion is desired, add 2 mL of HF (49%) to the reagents.
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
206
PHARMACEUTICAL AND NUTRACEUTICAL DIGESTION METHODS
REAGENTS REAGENTS
0.250 g 0.500 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 5 90 1 160 60 5 5 80
2 190 35 3 25 100 2 200 60 3 25 100
3 50 35 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
207
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PHARMACEUTICAL AND NUTRACEUTICAL DIGESTION METHODS
Wax
REAGENTS REAGENTS
0.500 g 1.000 g
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 170 30 5 5 90 1 170 60 5 5 80
2 200 30 3 30 100 2 220 60 3 20 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Accurately weigh the sample and transfer into the digestion vessel. Slowly add the reagents to the digestion vessel, rinsing the sample to the
bottom of the vessel. Gently swirl the mixture and wait approximately 10 minutes before putting the seal in place and closing the vessel.
To avoid foaming and sample loss when opening the vessels after digestion, wait until the vessels have cooled enough to be warm to the
touch and then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40% regardless of the
number of vessels digested.
208
TABLE OF CONTENTS
SUPPLEMENTAL
• Complexation of HF........................................................................................................................................................... 210
• New Vessel Conditioning................................................................................................................................................... 211
• Vessel Cleaning.................................................................................................................................................................. 212
• Vessel Re-Conditioning...................................................................................................................................................... 213
209
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SUPPLEMENTAL
Complexation of HF
REAGENTS REAGENTS
- -
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 1 90 1 160 60 5 1 80
2 190 30 1 15 100 2 190 60 1 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Add approximately 1 gram of boric acid to the digestion vessel for every 1 mL of HF used during digestion.
To avoid foaming when opening the vessels after complexation, wait until the vessels have cooled enough to be warm to the touch and
then vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40%, regardless of
the number of vessels digested.
210
SUPPLEMENTAL
REAGENTS REAGENTS
- -
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 2 90 1 160 60 5 2 80
2 200 30 2 10 100 2 210 60 2 10 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
Wait until the vessels have cooled enough to be warm to the touch and then vent the residual pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40%, regardless of
the number of vessels digested.
211
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SUPPLEMENTAL
Vessel Cleaning
REAGENTS REAGENTS
- -
TEMPERATURE PROGRAM
TEMPERATURE PROGRAM
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
1 160 30 5 2 90 1 160 60 5 2 80
2 200 30 2 15 100 2 210 60 2 15 100
3 50 30 1 15 0 3 50 60 1 30 0
4 - - - - - 4 - - - - -
5 - - - - - 5 - - - - -
PROCEDURE
To avoid foaming when opening the vessels after cleaning, wait until the vessels have cooled enough to be warm to the touch and then
vent the residual digestion pressure very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
The addition of H2O2 will increase the cleaning potential of the solution but can be left out, if desired.
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40%, regardless of
the number of vessels digested.
212
SUPPLEMENTAL
Vessel Re-Conditioning
REAGENTS REAGENTS
- -
Step Target Pressure Ramp Hold Power Step Target Pressure Ramp Hold Power
Temp [˚C] Limit [bar] Time [min] Time [min] [%] Temp [˚C] Limit [bar] Time [min] Time [min] [%]
PROCEDURE
Add the 25 mL of a 1% nitric acid solution and 3 mL of H2O2 to the digestion vessel.
After heating is complete, wait until the vessels have cooled enough to be warm to the touch and then vent the residual pressure
very slowly.
Always work in a fume hood wearing hand, eye and body protection since a large amount of gas can be produced during the
digestion process.
NOTES
This application is designed for the maximum number of vessels (sixteen 75 mL vessels or eight 100 mL vessels). Decrease the power
at the first step by 5% per vessel when using fewer than the maximum number of vessels. Minimum power is 40%, regardless of
the number of vessels digested. the number of vessels digested.
213
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PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or
(+1) 203-925-4602
www.perkinelmer.com
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