Expression Ready

TFP Retrovirus Controls

A lleleustrious
monomeric
mTFP-1
teal
is a
fluorescent
protein that can be easily detected
CAG promoter, human Ubiquitin C
promoter and PGK promoter) are
available for expression optimization.
unparalleled high titer and are ready
for incubation with target cells,
allowing for expression of the teal
using standard GFP filter sets. mTFP- Viral Particles are packaged with fluorescent protein mTFP-1.
1 is the brightest FP in the blue-cyan a proprietary process to achieve (See Product List below).
spectrum. Unlike many commonly
used fluorescent proteins, mTFP-
1 is a true monomer and is most Box 1 | Product List
likely to not interfere with function or
localization of its fusion partner. pCHAC-mTFP-1 ABP-SC-VIREMT5 5 vials, 10^7TU in 0.1ml per vial
Allele’s TFP Retrovirus Controls are pCHAC-mTFP-1 ABP-SC-VIREMT1 1 vial, 10^7TU in 0.1ml per vial
based on Phoenix2 system, one of the
pCHAC-CMV-mTFP-1 ABP-SC-VIRCMVT 5 vials, 10^7TU in 0.1ml per vial
most time-tested retrovirus systems
in use in over 3,000 laboratories pCHAC-SV40-mTFP-1 ABP-SC-VIRSVFT 5 vials, 10^7TU in 0.1ml per vial
worldwide for gene delivery. Currently, pCHAC-RSV-mTFP-1 ABP-SC-VIRRSVT 5 vials, 10^7TU in 0.1ml per vial
five viral promoters (MMLV LTR, pCHAC-HSV-TK-mTFP-1 ABP-SC-VIRHSVT 5 vials, 10^7TU in 0.1ml per vial
CMV promoter, SV40 promoter, RSV
pCHAC-EF1a-mTFP-1 ABP-SC-VIREFAT 5 vials, 10^7TU in 0.1ml per vial
promoter and HSV-TK promoter)
and five eukaryotic promoters (EF1a pCHAC-HbAPR-mTFP-1 ABP-SC-VIRHBAT 5 vials, 10^7TU in 0.1ml per vial
promoter, human beta-actin promoter, pCHAC-CAG-mTFP-1 ABP-SC-VIRCAGT 5 vials, 10^7TU in 0.1ml per vial
pCHAC-hUbC-mTFP-1 ABP-SC-VIRUBCT 5 vials, 10^7TU in 0.1ml per vial
pCHAC-PGK-mTFP-1 ABP-SC-VIRPGKT 5 vials, 10^7TU in 0.1ml per vial

Protocols Related Products

Storage: -80°C. Avoid repeated freeze thaw cycles ♦Phoenix2 pCHAC Retroviral
Vectors
Steps: - For Gene Expression
1. Plate target cells to 40-60% confluence in a 24-well plate before - For shRNA Expression
infection. - For miRNA Expression
2. Place mTFP-1-virus stock (virus titer: 1x108 IU/ml) in a 37°C water bath - mWasabiGFP Control
and before totally thaw transfer onto ice immediately. - LacZ Control
3. Make a serial dilution of 3-4 different multiplicity of infection
(MOI = infectious units/ cell number) to test the target cells. Example 1x104 ♦Gryphon Retroviral Packaging
TE671 cells in each well of a 24-well plate: Cell Lines
10MOI 20MOI 30MOI 60MOI - Ampho Cell
- Eco Cell
Culture Medium (ul): 198 197 195 189
Polybrene (2mg/ml): 1 1 1 1 ♦Gryphon Packaging Cell
Selection Medium
Virus (ul): 1 2 4 10
Total volume (ul): 200 200 200 200 ♦Phoenix2 Hexamethrine Bromide
4. Tilt the plate gently every 30 min. After 3hrs, add 1 ml fresh medium. for viral infection enhancement
5. After 48-72 hrs, evaluate mTFP-1 expression by using fluorescence
microscope or flow cytometry.
6. Choose the most efficient MOI with the least toxicity for iPS cell
derivation.

S afety Issues: Retroviral vectors should be handled using

NIH BSL-2 safety guidelines. For more information, please
see Biosafety in Microbiological and Biomedical Laboratories
(4th edition) which is available on the Web sites of the National
Institutes of Health at http://bmbl.od.nih.gov.
F or Research Use Only. Not for Diagnostic or
Therapeutic Use.
Purchase does not include or carry any right to resell or
transfer this product either as a stand-alone product or as
a component of another product. Any use of this product
other than the permitted use without the express written
authorization of Allele Biotech is strictly prohibited

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Viral Promoters

mTFP-1

mTFP-1

mTFP-1

mTFP-1

mTFP-1

Eukaryotic Promoters

mTFP-1

mTFP-1

mTFP-1

mTFP-1

mTFP-1

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