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Lampiran 1. Bagan kerja lengkap

1 Lembar daun sambiloto

SD1 SD2 SD3 SD4 SD5 SD6 SD7 SD8 SD9

Kultur jamur

Senyawa murni
Keterangan :

Dilakukan oleh mahasiswa jurusan Biologi FMIPA UNSRI (Apridinata, 2010)

Pengerjaan di laboratorium Kimia Organik FMPA UNSRI
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Lampiran 2. Skema pemisahan dan pemurnian senyawa murni hasil isolasi

Ekstrak etilasetat (2,7 g)

-KK, eluen : n-heksan : etil asetat (10:0-0:10)

- KLT

F1 (vial 1-30) F2 (vial 31-49) F3 (vial 50-60 ) F4 (vial 61-75)

-KK, eluen : n-heksan : etil asetat (4:6)

dan (3:7)
- KLT

F3.1 (vial 1-7) F3.2 (vial 8-15 ) F3.3 (vial 16-21) F3.4 (vial 22-23)

-KK, eluen : n-heksan : etil asetat (2:8)

Senyawa murni (7 mg)

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Lampiran 3.Gambar kultivasi jamur endofitik dari tanaman sambiloto

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Lampiran 4. Spektrum H-NMR senyawa SD2 (CD3OD, 500 MHz)

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Lampiran 5. Spektrum HMQC senyawa hasil isolasi (CD3OD, 500 MHz)

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Lampiran 6. Hasil analisis probit dengan program SPSS 11,5

PROBIT SD2
Probit
* * * * * * * * * * * * P R O B I T A N A L Y S I S * * * * *
* * * * * * *

DATA Information

4 unweighted cases accepted.

0 cases rejected because of missing data.
0 cases are in the control group.
0 cases rejected because LOG-transform can't be done.
MODEL Information

ONLY Normal Sigmoid is requested.

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
- - - - - - -

* * * * * * * * * * * * P R O B I T A N A L Y S I S * * * * *
* * * * * * *

Parameter estimates converged after 7 iterations.

Optimal solution found.

Parameter Estimates (PROBIT model: (PROBIT(p)) = Intercept +

BX):

Regression Coeff. Standard Error Coeff./S.E.

DOSIS ,26343 ,05756 4,57694

Intercept Standard Error Intercept/S.E.

,40776 ,10719 3,80391

Pearson Goodness-of-Fit Chi Square = 1,302 DF = 2 P

= ,522

Since Goodness-of-Fit Chi square is NOT significant, no

heterogeneity
factor is used in the calculation of confidence limits.

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
- - - - - - -

* * * * * * * * * * * * P R O B I T A N A L Y S I S * * * * *
* * * * * * *

Observed and Expected Frequencie

Number of Observed Expected
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DOSIS Subjects Responses Responses Residual

Prob

,00 100,0 64,0 65,827 -1,817

,65827
-1,00 100,0 56,4 55,738 ,642
,55738
-2,00 100,0 49,5 45,260 4,280
,45260
-3,00 100,0 32,1 35,103 -3,013
,35103

* * * * * * * * * * * * P R O B I T A N A L Y S I S * * * * *
* * * * * * *

Confidence Limits for Effective DOSIS

95% Confidence Limits

Prob DOSIS Lower Upper

,01 4,179422E-11 8,721793E-18 ,00000

,02 4,528175E-10 5,579558E-16 ,00000
,03 2,053407E-09 7,796111E-15 ,00000
,04 6,402853E-09 5,662957E-14 ,00000
,05 ,00000 2,839447E-13 ,00000
,06 ,00000 1,119309E-12 ,00000
,07 ,00000 3,724329E-12 ,00000
,08 ,00000 1,092230E-11 ,00001
,09 ,00000 2,904520E-11 ,00001
,10 ,00000 7,142908E-11 ,00001
,15 ,00000 2,946331E-09 ,00006
,20 ,00002 ,00000 ,00020
,25 ,00008 ,00000 ,00059
,30 ,00029 ,00001 ,00157
,35 ,00098 ,00005 ,00398
,40 ,00309 ,00033 ,01021
,45 ,00944 ,00184 ,02813
,50 ,02832 ,00825 ,09242
,55 ,08494 ,02852 ,39376
,60 ,25932 ,08111 2,12990
,65 ,82190 ,21109 13,80198
,70 2,77192 ,54166 105,61511
,75 10,29274 1,44267 985,54458
,80 44,35831 4,19363 12136,42421
,85 243,49901 14,29902 230442,17850
,90 2074,84176 65,96628 9493657,65896
,91 3481,17600 95,28635 23342384,8749
,92 6107,68389 142,00768 62061318,8629
,93 11332,69468 220,09620 181972580,496
,94 22602,53727 358,84006 605377211,912
,95 49671,58857 626,24385 2385976812,90
,96 125272,65854 1203,82259 11961388292,5
,97 390620,19343 2685,93407 86870231973,8
,98 1771358,88562 7796,12584 1213583114147
,99 19191705,3802 41721,07051 7,761964E+13
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Probit Transformed Responses

,4

,2

-,0

-,2

-,4
Probit

-,6
-3,5 -3,0 -2,5 -2,0 -1,5 -1,0 -,5 0,0 ,5

Log of DOSIS
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