ขข้อมมูล DPPH *ททที่ 520 nm

ววันททที่ 27/9/56 solvent : DMSO

trolox solvent DPPH : methanol
conc. sample absorbance © Blank absorbance (D)
mcg/ml 1 2 3 average 1 2 3 average
3.905 1.0187 0.9221 0.936 0.95893333 0.0341 0.0359 0.0353 0.0351
7.81 0.938 0.9155 0.8898 0.91443333 0.0376 0.0357 0.0356 0.0363
15.63 0.8566 0.8584 0.835 0.85 0.0365 0.0383 0.0337 0.03616667
31.25 0.6952 0.659 0.6672 0.6738 0.0361 0.0358 0.035 0.03563333
62.5 0.3141 0.1418 0.1459 0.2006 0.0366 0.0367 0.0364 0.03656667
125 0.0657 0.0687 0.0705 0.0683 0.0349 0.0344 0.0352 0.03483333
250 0.0633 0.0676 0.06 0.06363333 0.034 0.0345 0.0321 0.03353333
500 0.0617 0.0611 0.0616 0.06146667 0.0347 0.0347 0.0351 0.03483333

positive control (A) negative control (B)

No. 1 2 3 average 1 2 3 average
1 0.8122 0.9528 0.9491 0.9047 0.0365 0.037 0.0378 0.0371

A-B C-D (A-B)-(C-D) %inhibition final conc. %inhibition

0.8676 0.92383333 -0.0562333333 -6.4814815 0.3905 -6.4814815
0.87813333 -0.0105333333 -1.2140771 0.781 -1.2140771
0.81383333 0.0537666667 6.19717228 1.563 6.19717228
0.63816667 0.2294333333 26.4445981 3.125 26.4445981
0.16403333 0.7035666667 81.0934378 6.25 81.0934378
0.03346667 0.8341333333 96.1426156 12.5 96.1426156
0.0301 0.8375 96.5306593 25 96.5306593
0.02663333 0.8409666667 96.930229 50 96.930229

120
f(x) = - 0.4149299095x^2 + 14.6336198685x - 11.5611228362
100 R² = 0.9570980147

80

60
IC50 4.88
40

20

0
0 5 10 15 20 25 30
-20

a b c -b b2 4ac- 2a b2-4ac /b2-4ac

-0.4149 14.634 -61.561 -14.634 214.153956 -102.16664 -0.8298 111.98732 10.5824062
 ขข้อมมูล DPPH *ททที่ 520 nm
ววันททที่ 30/10/56 solvent : DMSO
trolox solvent DPPH : methanol
conc. sample absorbance © Blank absorbance (D)
mcg/ml 1 2 3 average 1 2 3 average
3.905 0.9573 0.826 0.8433 0.87553333 0.036 0.0389 0.0363 0.03706667
7.81 0.8431 0.9144 0.8397 0.86573333 0.0364 0.0409 0.0376 0.0383
15.63 0.7964 0.8007 0.7607 0.78593333 0.0377 0.0409 0.0348 0.0378
31.25 0.6989 0.6832 0.6806 0.68756667 0.0392 0.0377 0.0386 0.0385
62.5 0.3724 0.3994 0.4695 0.41376667 0.0393 0.04 0.0356 0.0383
125 0.0959 0.0954 0.0876 0.09296667 0.037 0.0362 0.0385 0.03723333
250 0.0626 0.066 0.0655 0.0647 0.0404 0.0389 0.0356 0.0383
500 0.0639 0.063 0.0605 0.06246667 0.0369 0.0362 0.0362 0.03643333

positive control (A) negative control (B)

No. 1 2 3 average 1 2 3 average
1 0.8087 0.8777 0.876 0.85413333 0.0379 0.0363 0.037 0.03706667

A-B C-D (A-B)-(C-D) %inhibition final conc. %inhibition

0.81706667 0.83846667 -0.0214 -2.6191253 0.3905 -2.6191253
0.82743333 -0.0103666667 -1.2687663 0.781 -1.2687663
0.74813333 0.0689333333 8.43668407 1.563 8.43668407
0.64906667 0.168 20.5613577 3.125 20.5613577
0.37546667 0.4416 54.0469974 6.25 54.0469974
0.05573333 0.7613333333 93.1788512 12.5 93.1788512
0.0264 0.7906666667 96.7689295 25 96.7689295
0.02603333 0.7910333333 96.813805 50 96.8138055

120

100 f(x) = - 0.3113940891x^2 + 12.0649805718x - 9.9933078529

R² = 0.9976348365
80

60 IC50 5.86

40

20

0
0 5 10 15 20 25 30
-20
a b c -b b2 4ac- 2a b2-4ac /b2-4ac
-0.3114 12.065 -59.9933 -12.065 145.564225 -74.727654 -0.6228 70.8365705 8.41644643
 IC50
ครวังรั้ ทท1
ที่ ครวังรั้ ททที่ 2 Mean 0.00537
0.00488 0.00586 SD 0.000693
mcg/ml

-b+
4.88
-b-
30.39
mcg/ml

-b+
5.86
 -b-
32.89