Expression Ready

YFP Retrovirus Controls

L anYFP is a yellow fluorescent

protein that can be easily detected
using standard GFP filter sets. The
promoter, human beta-actin promoter,
CAG promoter, human Ubiquitin C
promoter and PGK promoter) are
a proprietary process to achieve
unparalleled high titer and are ready
for incubation with target cells,
lancelet fluorescent proteins are a available for expression optimization. allowing for expression of the Yellow
newly discovered, promising group of Viral Particles are packaged with fluorescent protein LanYFP.
fluorescent proteins introduced to the (See Product List below).
market exclusively from Allele Biotech.
These proteins were discovered in
Box 1 | Product List
Lancelets (also called amphioxus), a
species of small fish that are usually pCHAC-LanYFP ABP-SC-VIRELY5 5 vials, 10^7TU in 0.1ml per vial
found buried in sand in shallow
parts of temperate or tropical seas. pCHAC-LanYFP ABP-SC-VIRELY1 1 vial, 10^7TU in 0.1ml per vial
Preliminary tests show that LanYFP is pCHAC-CMV-LanYFP ABP-SC-VIRCMVY 5 vials, 10^7TU in 0.1ml per vial
the brightest FP on the market. pCHAC-SV40-LanYFP ABP-SC-VIRSVFY 5 vials, 10^7TU in 0.1ml per vial
Allele’s YFP Retrovirus Controls pCHAC-RSV-LanYFP ABP-SC-VIRRSVY 5 vials, 10^7TU in 0.1ml per vial
are based on Phoenix2 system, pCHAC-HSV-TK-LanYFP ABP-SC-VIRHSVY 5 vials, 10^7TU in 0.1ml per vial
one of the most time-tested
retrovirus systems in use in over pCHAC-EF1a-LanYFP ABP-SC-VIREFAY 5 vials, 10^7TU in 0.1ml per vial
3,000 laboratories worldwide for pCHAC-HbAPR-LanYFP ABP-SC-VIRHBAY 5 vials, 10^7TU in 0.1ml per vial
gene delivery. Currently, five viral pCHAC-CAG-LanYFP ABP-SC-VIRCAGY 5 vials, 10^7TU in 0.1ml per vial
promoters (MMLV LTR, CMV
pCHAC-hUbC-LanYFP ABP-SC-VIRUBCY 5 vials, 10^7TU in 0.1ml per vial
promoter, SV40 promoter, RSV
promoter and HSV-TK promoter) and pCHAC-PGK-LanYFP ABP-SC-VIRPGKY 5 vials, 10^7TU in 0.1ml per vial
five eukaryotic promoters (EF1a

Protocols Related Products

Storage: -80°C. Avoid repeated freeze thaw cycles ♦Phoenix2 pCHAC Retroviral
Vectors
Steps: - For Gene Expression
1. Plate target cells to 40-60% confluence in a 24-well plate before - For shRNA Expression
infection. - For miRNA Expression
2. Place LanYFP-virus stock (virus titer: 1x108 IU/ml) in a 37°C water bath - mWasabiGFP Control
and before totally thaw transfer onto ice immediately. - LacZ Control
3. Make a serial dilution of 3-4 different multiplicity of infection
(MOI = infectious units/ cell number) to test the target cells. Example 1x104 ♦Gryphon Retroviral Packaging
TE671 cells in each well of a 24-well plate: Cell Lines
10MOI 20MOI 30MOI 60MOI - Ampho Cell
- Eco Cell
Culture Medium (ul): 198 197 195 189
Polybrene (2mg/ml): 1 1 1 1 ♦Gryphon Packaging Cell
Selection Medium
Virus (ul): 1 2 4 10
Total volume (ul): 200 200 200 200 ♦Phoenix2 Hexamethrine Bromide
4. Tilt the plate gently every 30 min. After 3hrs, add 1 ml fresh medium. for viral infection enhancement
5. After 48-72 hrs, evaluate LanYFP expression by using fluorescence
microscope or flow cytometry.
6. Choose the most efficient MOI with the least toxicity for iPS cell
derivation.

S afety Issues: Retroviral vectors should be handled using

NIH BSL-2 safety guidelines. For more information, please
see Biosafety in Microbiological and Biomedical Laboratories
(4th edition) which is available on the Web sites of the National
Institutes of Health at http://bmbl.od.nih.gov.
F or Research Use Only. Not for Diagnostic or
Therapeutic Use.
Purchase does not include or carry any right to resell or
transfer this product either as a stand-alone product or as
a component of another product. Any use of this product
other than the permitted use without the express written
authorization of Allele Biotech is strictly prohibited

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Viral Promoters

LanYFP

LanYFP

LanYFP

LanYFP

LanYFP

Eukaryotic Promoters

LanYFP

LanYFP

LanYFP

LanYFP

LanYFP

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