Biochemistry 1 March 7 Exam, 2018 Name:

Score: Attendance Forever Number:

YOU MUST fill in the answer on each question line______. You must write clearly capital A, B, C, or D in the
respective line_______.

 Mol weight of amino acid, peptide mol weight calculation ( in class slides)
 pI, pH
 Edman degradations ( in class slides and others)

If the polypeptide is small ( 50 AAs or less) you can determine the sequence using repeated cycles of
Edman Degradation • Here, Phenylisothiocyanate (PITC) reacts with the N-terminal amino group under
alkaline conditions to form a phenylthiocarbamoyl (PTC) adduct. • The peptide bond adjacent to this
labeled terminus is cleaved under acidic conditions (Thifluoroacetic acid) – This treatment cleaves only
the peptide bond of interest! • This derivative is then extracted in organic solvent and identified
 Protein denaturation ( in class slides and others)
 Fibrous Protein (slides summary in table and others)
 Globular protein (slides and others)
 Oxygen binding curve, T , R state terms, transition ( in class slides and others)
 Actin monomer, F-actin, binding, myosin structure, binding ( in class slides and others )
 Muscle contraction; ATPase; each protein function ( in class slides and others)
 Regulation of oxygen binding to hemoglobin by BPG, CO2, CO, and more; Bohr effect. ( in class slides and
text book)
 Passage questions: MCAT style
 peptide bonds
 amino acids character and their effect on , their frequency to appear on 2D , 3D structure;
 calculate pH ( chapter 2) ( in class slides and others)
 Kd, Ka, relation of Kd, Ka, calculation of theta ( in class slides and others), calculation of ligand binding %
 Antibodies structure and application in biochemistry research methods; IP, protein seperation methods.
( in class slides and others)
 P50, relation to Kd, Ka, calaulation of ligand binding, percentage of ( in class slides and others)

1
 n
Hill coefficient, h , positive, negative cooperativity, Hill plot ( in class slides and others)
 Extra questions: (slides and higher level knowledge), antibody
 SDS page gel
 Antibody structure, function, generation