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Purpose:
The purpose of this is lab is to Understand what a bacterial plasmid is and how it is
used in biotechnology, and . Understand how genes are used to make protein in a cell.
Hypothesis:
If E. Coli are to accept the pGlo then they should resist the antibiotic and glow under the
UV light.
Procedure:
All procedure is referred to the pGlo lab under BABEC.
Observation:
● I noticed that adding the materials with the pipet is really difficult because risk of
contamination.
● During the lab the room that we stayed in was really warm and smelly.
● I noticed that we were adding very small amounts of materials by using the
micropipette.
● Does not include Antibiotic ● Does not include antibiotic resistant gene
resistant Gene ● LB and AMP present
● LB only
Analysis:
● DNA+, LB ● DNA-, LB
○ It should show growth. ○ It should show growth.
○ It should not glow because ○ It should not glow because
there is no pGlo in the there is no pGlo in the
bacteria. bacteria.
Conclusion:
Base on this lab I found out that if you add the pGlo into a bacteria it will glow under a
UV light. This lab was designed to help the student to understand what a bacterial
plasmid is, and how is it used in biotechnology. In addition to understand how genes
are used to make protein in a cell. I found out that pGlo can make a bacteria glow
because clearly based on the experiment when I added the pGlo to the E.Coli the end
result was the bacteria to glow under the UV light.