 Opsi Bahan :

1. Madu
2. Teh hijau herbal
3. Obat diet pake sibutramin HCl
 Metode : FTIR

1. MADU
Jurnal “adulteran madu”
Application of FTIR-HATR spectroscopy and multivariate analysis to the
quantification of adulterants in Mexican honeys
Bahan :

 Madu diambil dari 4 bagian negara yang berbeda :

19 from Chiapas, 19 from Oaxaca,20 from Estado de México, and 20 from Morelos

 Bahan pemanis yang digunakan sebagai adulteran :

high fructose corn syrup (HFCS55) dari CPI Ingredients (México); inverted
cane sugar (high inversion) dari Inverazúcar S.A., México and corn syrup dari
Productos de Maíz S. A. de C.V. (México).

 Jarak pecampuran sampel madu untuk menutupi keduanya : 2-27 %

 For the adulterated honey set with HFCS 55, 30 samples
in the range between 2% and 60% (w/w) were used as the calibration
set,
 15 samples prepared in the same concentration region
but with different concentrations of the calibration set were used
as the validation set.
 For the adulterated honey with inverted sugar
it was only required the preparation of 25 samples in the range between
2% and 50% (w/w) for the calibration set. For this system, 13
additional samples were used as the validation set. Finally for the
adulterated honey with corn syrup, it was required the preparation
of 49 samples in the range 2–50% (w/w) for the calibration set,
using 25 additional samples for the validation set. The range of
concentrations used for the former adulterants were similar to the ones used by
Kelly, Petisco, and Downey (2006).

FTIR
Perkin Elmer 1600 FTIR Spectrometer (Massachusetts, USA) mengukur
Menggambarkan suatu senyawa yang memiliki beberapa hidrogen normal
(protium) diganti dengan deuterium isotop berat. Detektor trigliserin sulfat.
The sampling station was
equipped with an overhead attenuated total reflection accessory
(ATR), comprising of transfer optics within the chamber through
which infrared radiation was directed to a detachable zinc selenide
crystal mounted into a shallow through for sample containment.
The crystal geometry was a 45 parallelogram with
mirrored
angled faces, with nominal ten internal reflections. Sixty four scans
were coadded at a nominal resolution of 4 cm1 in the spectral region
of 4000–650 cm1 .Single beam spectra of the samples were
collected and rationed against a background of water and presented
in absorbance units. Samples were applied to the ATR crystal
to give total crystal coverage; the crystal was cleaned between
samples with water and dried with lens cleaning tissue. The
cleaned crystal was carefully examined visually to ensure that no
residue from the previous sampling was retained on the crystal
surface and checked with the background spectrum. Spectra were
collected and used for multivariate analysis.