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Summary. Water vapor and CO, exchange characteristics tor had higher rates of photosynthesis, respiration, higher
were studied in Phoradendron villosum, a hemiparasitic mis- water deficits, and lower osmotic potentials than its host
tletoe and its host, Quercus lobata. The hemiparasite had Acacia grasbyi. Rates of transpiration were slightly higher
stomatal conductances equal to or higher than the host in the host.
but a much lower capacity to fix carbon. Respiration was Here I describe the carbon dioxide exchange and tran-
high in the mistletoe relative to maximum photosynthesis spiration of a hemiparasite Phoradendron uillosum Nutt.,
because of the high leaf specific weight (weight per unit the oak mistletoe, in relation to that of its host, Quercus
area) in this species. The temperature optima for photosyn- lobata N6e., the California valley oak. This work shows
thesis were similar in both species although photosynthesis that the hemiparasitic P. villosum has a significantly lower
in Phoradendron declined more steeply below 20 degrees capacity to fix carbon than its host despite a relatively high
C. Consequences of the high conductance and low rate of rate of transpiration. These results are considered in the
photosynthesis in the mistletoe include a low water-use effi- context of the host-parasite relationship and plant water-
ciency and a relatively high internal concentration of CO,. use efficiency.
Introduction Methods
Mistletoes are hemiparasites. As such, they rely upon their Phoradendron villosum occurs on Quercus lobata and other
hosts for a source of water and minerals but produce, to species of oak throughout California (Munz 1959). Work
at least a certain extent, their own supply of assimilate described here was carried out at the Jasper Ridge Biologi-
(Kuijt 1969). Despite this unusual partitioning of plant cal Preserve of Stanford University. Jasper Ridge is located
water and carbon economy, there have been few recent in the foothills of the Santa Cruz mountains 12 km west
studies of the water relations or photosynthetic capacities of Palo Alto, California, U.S.A. The mediterranean-type
of mistletoes or other hemiparasitic species. Early work cliinate consists of hot, dry summers and cool, wet winters.
(Kamerling 1914) indicated that the European mistletoe CO, and water vapor exchange measurements of host
Viscum album had an unusually high rate of transpiration and hemiparasite were carried out in the field in July and
although later work (Hartel 1937) showed that this rate August of 1982 with a portable steady-state gas exchange
was quite variable. Glatzel (1983) has recently reviewed system described by Field et al. (1982). This system consists
much of the early work dealing with mistletoe water rela- of a temperature-controlled cuvette, infrared gas analyzer
tions and has shown that stomata of the deciduous mistle- and water-filtered light source. Humidity is added to the
toe Loranthus europaeus close at a more negative water cuvette by leaf transpiration and controlled by varying the
potential than those of its host Quercus robur. flow rate of dry air containing an ambient (- 320 ~111)con-
Our knowledge of the photosynthetic capacities of hemi- centration of CO,. Ambient levels of CO, are maintained
parasitic plants also remains limited. Freeland (1943) dem- by adding a stream of 1% CO, in nitrogen to the cuvette
onstrated net CO, uptake by an American mistletoe while to replace carbon dioxide assimilated by the leaf. Replicate
Seledzhana and Galan-Fabian (1961) showed with I4CO2 meaiurements were made on several leaves of each species
feeding experiments that Viscum also fixes carbon. They for each experimental manipulation of light, temperature
noted that the mistletoe translocates little photosynthate or vapor concentration gradient. Typical response curves
to its host, suggesting that the Viscum infection is not mu- are shown in the figures.
tualistic. Hull and Leonard (1964) used the same technique Transpiration (E), net photosynthesis ( A ) , stomatal con-
to show that photosynthetic capacity in several species of ductance (g)and other gas exchange parameters were calcu-
Phoradendron was greater than in several species of the lated as described in Field et al. (1982).
, ,
dwarf mistletoe Arceuthobium. Phoradendron apparently Stomata1 pore lengths and densities were measured from
did not utilize host photosynthate wtule Arceuthobium did. acetate negatives (clear nail polish) of silicone impressions
In a more comprehensive study, Hellmuth (1971) (Xantopren blue, Unitek Corporation, Monrovia, Ca.) of
showed that the Australian stem hemiparasite Amyema nes- fresh leaf material.
Quercus
B
Phoradendron Quercus
Fig
dro
tive
July 28
Auercus
July 27
Phoradendron
1982
- 0
10 15 X) 25 30 35 40 U
Leaf temperature (OC)
0 4 8 12 16 20 24 28 32
Fig. 3. Normalized net photosynthesis as a function of temperature
in Phoradendron and Quercus. PAPF = 1,000 pmol m-' s- ', Vapor concentration gradient
A W = 5 mmol H 2 0 mol- air ' (mmol H20 / mol air)
Fig. 4A-D. Net photosynthesis, transpiration, stornatal conduc-
tance to water vapor and ratio of intercellular CO, to external
Despite the large differences in net CO, uptake between CO, as functions of leaf-air vapor concentration gradient. PAPF=
host and hemiparasite, stomatal conductances in both spe- 1,000 pmol m-' s - l , leaf temperature=30 degrees C
cies are similar (Fig. 2 B). Stomata are larger but less numer-
ous in Phoradendron than Quercus (Table 2). Stomatal con-
ductances are discussed in more detail below with reference while the net rate of CO, uptake in the mistletoe is only
to the water use efficiency of both species. one-half that of the oak. Because of the high conductance
and low photosynthetic rate, even at a AW of greater
than 30 mmol H,O/mol air, the internal concentration of
Temperature
CO, in Phoradendron is over 90% of that existing outside
The responses of net photosynthesis in Phoradendron and of the leaf. This contrasts sharply with the internal concen-
Quercus to temperature are qualitatively similar (Fig. 3). trations of CO, in Quercus (Fig. 4 D).
The optimum range of temperatures for maximum photo- The very high rate of transpiration combined with the
synthesis is apparently somewhat broader in the oak than low rate of net photosynthesis in Phoradendron means that
in the mistletoe, however. Greater than 90% of the maxi- E/A, the instantaneous water-use efficiency, of this species
mum rate of photosynthesis is observed in the oak with is very low. As Figs. 5A and B show, compared to its host,
leaf temperatures ranging from 15 degrees C (the lowest Phoradendron is indeed a profligate user of water. At a
leaf temperature measured) to approximately 32 degrees C. light intensity of 1,000 pmol/m2s and depending on the va-
The temperature response of the mistletoe was similar al- por concentration gradient, Phoradendron transpires from
though it appeared to drop off steeply below about 20 de- 3 to 5 times as much water as its host at the same AW
grees C. Wagener (1957) has noted the sensitivity of Phora- per equivalent unit of photosynthesis. Figure 5 b shows in-
dendron to low temperatures. stantaneous water use efficiency (EIA) for the two species
+
as ligth varies but at a constant A Wof 8.5 1 mmol H,O/mol
air. There is a large difference in water use efficiency (mols
Vapor concentration gradient
water transpired per mol carbon assimilated) even at the
In contrast to Quercus, net CO, uptake in Phoradendron low light intensities which Phoradendron often experiences.
declines only slightly with an increasing leaf-air vapor con- Cowan and Farquhar (1977) have suggested that the
centration gradient (Fig. 4A). This is not because Phoraden- slope of the relationship between E and A (dE/dA) provides
dron stomata fail to respond to an increasing AW, but be- another test of the efficiency of water use in a plant. In their
cause of the high conductance of this species relative to model, given a certain amount of water to be transpired
its photosynthetic capacity (Fig. 4C). At a light intensity throughout a day, carbon gain is maximized (water-use effi-
of 1,000 ~mol/m2s,the stomatal conductance of Phoraden- ciency is optimized) if dE/dA is constant. dE/dA in Phora-
dron is approximately 2 and one-half times that of its host dendron and Quercus was evaluated by the method of Far-