Noviplex™ Duo Cards for “Lab-on-a-

Card” Plasma Collection and Sample

Preparation
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NoviplexTM Plasma Prep Cards are simple, powerful

microsampling tools used to collect volumetric samples of
plasma from non-volumetric applications of whole blood
in just minutes. An un-measured application from a finger-
stick is all that is required to collect a volumetric plasma
sample in only three minutes. Noviplex Cards can be used
as a single step, solid-phase method for sample
preparation and analyte extraction, eliminating the need
for time consuming techniques such as solid phase
extraction, centrifugation and evaporation.

Noviplex technology also provides exceptional

reproducibility independent of hematocrit, 99 percent
removal of red blood cells, and rapid sample preparation
for MS-based analysis while maintaining assay
reproducibility and selectivity.

Properties of the Noviplex Card

 99% removal of red blood cells (based on Hb levels in plasma).
 Plasma sampling volumes of 2.5 µL (7.6 µL DUO).
 Inter-card CV of ~1% (~3% DUO) in plasma collection volume at constant hematocrit
(HCT).
 Inter-card CV of 2% collection volume variation with 20% to 71% HCT
 Proteins are deposited as a monolayer upon drying.
 Metabolites are present in the protein monolayer.

Significant advantages of Noviplex Card technology

 Rapid sample preparation for MS-based analysis, while maintaining assay
reproducibility and selectivity.
 Volumetric plasma sample collection using a single drop of whole blood.
 Specimen volumes as low as 25 µL.
 Noviplex cards simplify sample preparation. Eliminates need for solvent extraction,
centrifugation and evaporation.
 Exceptional reproducibility independent of hematocrit.
The new Noviplex™ Duo Plasma Prep Card for preparing two plasma samples
from a single application of blood. Like the original Noviplex Card, the Duo
prepares a blood sample anytime and anywhere without the need for a power
source. The Noviplex Cards prepare a precise volume of plasma from a variable
amount of blood, and stabilize the plasma samples in minutes for shipment
worldwide without the need for dry ice. The technology is ideal for use with LC-
MS/MS analysis.

Noviplex Duo Cards expand the application of the original Noviplex Card by
enabling two samples to be generated from a single application of whole
blood. The new Duo Card collects about 8 microliters of plasma, which makes it
well suited to many LC/MS assays. The Duo Card provides the ability to perform
two different types of LC-MS assays with a single application of blood. For
instance, it is possible measure both calcium and vitamin D in a blood sample,
which provides better insight to bone health than just vitamin D alone.

Noviplex™ Plasma Prep

Cards
Standard Duo
Number of plasma samples
1 2
generated
Minimum volume required 25 µl 60 µl
Volume collected per plasma
2.5 µl 3.8 µl
sample
Total volume collected per card 2.5 µl 7.6 µl
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