Hematology

Specimen:
 2 cc venous blood in EDTA tube (preferable esp if > 1 test requisted)
 capillary tube filled from Finger prick (Hold heparinized cap tube near calibration mark & touch the other
end into drop of blood. Allow blood to flow up to calibration mark.Never squeeze fingertip tightly.
 When blood reach calibration mark, remove the tube from the finger, holding it level so that blood will not
flow out of the outer end. Note: If the tube accidentally filled beyond calibration mark, remove excess
blood by gently dabbing the wet tip with absorbent paper.

pt waiting time:

Test Technic
Hb/PCV I. microHct method
1. fill microhct tube (~3/4 tube length)
2. plug empty end with clay (~ 1 cm long)
3. spin at 3000 rpm/5 min
4. read using Hct scale by placing bottom of blood column at zero & top at 100% (ie; interface bn tube
sealer & packed cells column is exactly at "O" mark of Hct scale. Read the mark on the printed scale adjacent
to interface bn plasma & packed cells column)
Hct= top of packed cell column (i.e., % of length of packed cell column/whole length of sample)
II. Sahli method
* fill Sahli tube é 0.1N Hcl to lowest mark 0.02 gram (mark 20)
* add 20 l (20 mm2) blood (sahli pipete to 0.02 mark, wipe pipete). mix é rod.
* allow tube to stand 10 min
* dilute é DW drop by drop, wirh continous mixing by rod until color match comparator.
WBC * prepare tubes contain 0.38 ml WBC diluent
1. add 20 l (0.02 ml) blood+ 0.38 ml diluent. Mix well (avoid bubbles)
2. put coverslip on counting chamber
3. fill (but not overfill) counting chamber.
4.keep chamber in wet Petri dish/2 min (cells settle)
5. wipe back of chamber, count under 10x or 4x/  illumination (count cells toching Rt line but not left line,
top line but not bottom line
WBC/mm3 = WBC counted in 4 W sq x 50 (or cells counted in 1 W sq x 200)
ESR I. conventional:
2 cc blood (in EDTA) + 0.5 cc NS mix well fill ESR tube exactly to mark “0”  put upright in ESR rack
read upper level of RBC (drak red level) after 1 hr
II. micro ESR
1. fill completely, standard heparinized microHct tube.
2. close one end of tube with clay
3. fix the tube vertically by sticking plaster
4. read ESR (fall in RBC after 1 hr to nearest mm.
Diff 1. make blood film (as usual) dry quickly (fan or shaking)
WBC 2. stain:
i. cover dry blood film é leishman stain/1 min
ii. add same amount of DW, mix by gentle blowing
iii. leave 12 min
iv. wash with DW (few seconds)
v. wipe back of slide, allow to dry.
3. counting
smear a drop of oil on stained blood film, count 100 cell (use tallying or table é 100 sq (10 rows x 10
columns) N= netrophil, B= basophil, E = eosinophil, L= lymphocytes, M= monocytes
4. report results as % of each WBC type