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3D Microscopy for

Axioskop

Operating Manual
Copyright 3D Microscopy

Knowledge of this manual is required for the operation of the instrument. Would you therefore
please make yourself familiar with the contents of this manual and pay special attention to hints
concerning the safe operation of the instrument.
The specifications are subject to change.
 Unless expressly authorized, forwarding and duplication of this document, and the utilization and
communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of granting of patents or registration of a utility model.

Issued by: Carl Zeiss


Mikroskopie
D-07740 Jena
Telephone: (03641) 64-16 16
Telefax: (03641) 64-31 44
Internet: mikro @ zeiss.de
http://www.zeiss.de

Number of this manual: B 40-022-7 e


Date of issue: 07/98

2 B 40-022-7 e 07/98
3D Microscopy Contents

Contents

1 Notes on safety ................................................................................................... 4


1.1 General.............................................................................................................................. 4
1.2 Regulations concerning instrument safety and electromagnetic compatibility (EMC)........... 4
1.3 Notes on unpacking, transport and storage........................................................................ 5
1.4 Hints on use....................................................................................................................... 5
1.5 Notes on maintenance ....................................................................................................... 5
2 General ................................................................................................................. 6
2.1 Application ........................................................................................................................ 6
2.2 Function............................................................................................................................. 7
2.2.1 Eyepiece version................................................................................................................. 7
2.2.2 Monitor version.................................................................................................................. 8
2.2.3 Combination of eyepiece and monitor version ................................................................ 10
2.3 Maintenance and care ..................................................................................................... 11
2.3.1 General............................................................................................................................ 11
2.3.2 Cleaning of optical components....................................................................................... 11
2.3.3 Change of voltage and fuses............................................................................................ 11
2.3.4 Troubleshooting............................................................................................................... 12
3 Technical Data ................................................................................................... 13

Certification in accordance with DIN EN ISO 9001 and DIN EN 46001

EC Declaration of Conformity

Annex (optional)

3D microscopy for Axiotron 2


3D microscopy for Axiotech 100
3D microscopy for Axiotech 100 vario
3D microscopy for Axiovert 100/135/135 M
3D microscopy for Axiovert 25 CA
3D microscopy for Axioplan 2/Axiophot 2
3D microscopy for Axioskop
3D microscopy for Axioskop 2

B 40-022-7 e 07/98 3
Notes on safety 3D Microscopy

1 Notes on safety
1.1 General
Please make yourself familiar with the contents Caution!
of these operating instructions before Non-observance of the safety notes constitutes
attaching and starting up the components for a hazard for the user.
3D microscopy. Additional information is
available from our maintenance service or from
authorized agencies. Caution!
Non-observance of the safety notes constitutes
For the function of 3D microscopy in
a hazard for the instrument.
combination with the
 Axiotron 2
 Axiotech 100 Caution!
 Axiotech 100 vario Disconnect the instrument from the line before
 Axiovert 100/135/135 M opening it.
 Axiovert 25 CA ☞ Note!
 Axioplan 2/Axiophot 2 Notes which must be observed.
 Axioskop
 Axioskop 2
1.2 Regulations concerning
please see the operating instructions of these instrument safety and
microscopes.
electromagnetic
When using video components from KAPPA,
please comply with the supplied manual "Color compatibility (EMC)
TV camera for microscopy and 3D images".
The components for 3D microscopy have been
Special regard must be paid to the notes on designed and tested in compliance with EN
instrument safety included in this manual. 61010, Part 1 (VDE 0411). From the safety
To guarantee the safe operation and function viewpoint, they left the factory in a perfect
of the components for 3D microscopy, it is state.
necessary under any circumstances to take the They meet the requirements of the EC directive
precaution measures and observe the warnings 89/336/EC and the EMC legislation of
contained in the operating instructions. November 9th 1992 and the legal regulations
The following symbols are used for the for accident prevention.
warnings: The components for 3D microscopy meet the
EC requirements and are CE-labelled.

4 B 40-022-7 e 07/98
3D Microscopy Notes on unpacking, transport and storage

1.3 Notes on unpacking,  Cable connections may only be made if the


transport and storage various components are switched off.
 For the video components from KAPPA, it is
The components for 3D microscopy must be absolutely necessary to use only the
transported in their original packaging. It is connectors listed in the manual "Color TV
recommended to use the original packaging camera for microscopy and 3D images".
also for storage.
 Make sure that the cables are not bent or
The transport and storage temperatures must laid out in too narrow a radius.
comply with those listed under "Technical
Data".
 The optical components must be protected
from dirt and mechanical damage;
When unpacking the components which are special care must be taken to prevent the
equipped with polarizing foil (3D illumination polarizing foils of the 3D illumination
shutter, eyepieces with 3D shutter), make sure shutter from being touched.
to be particularly careful, since the foil can be  The components for 3D microscopy must be
easily scratched. protected from impact and humidity.

1.4 Hints on use 1.5 Notes on maintenance


 The components for 3D microscopy can  To enable 3D microscopy to function
only be used with halogen lamps up to perfectly, it is necessary to use only
max. 100 W. components and spares marketed or
admitted by us. When in doubt, please
contact our service staff.

 The power plug must be inserted in a


socket featuring a grounding (earth)
contact.  With the exception of the work described in
The grounding effect must not be reduced the "Care and maintenance" chapter, the
by an extension cable which does not have user must not perform any repairs or
a protective ground wire. changes himself. We would like to
emphasize that all other changes and
repairs must be performed by specially
authorized personnel.
 Prior to connection to the line, check  The manufacturer is not liable for damage
whether the voltage set on the selector at caused by unauthorized operation. This
the rear of the 3D-CONTROL DAS-3 unit forfeits all the claims against warranty.
complies with the line voltage; it may be
necessary to change from 230 V to 115 V.
The change to 115 is only required for the
3D-CONTROL DSA-3 unit.
For further details, see "Care and
Maintenance" on page VIII.

B 40-022-7 e 07/98 5
General
Application 3D Microscopy

2 General
2.1 Application
3D microscopy permits the stereoscopic viewing 3D microscopy is ideal for the following
of microscope specimens and objects applications in biomedicine:
 up to highest magnifications  micromanipulation
 using all objectives  patch-clamp technique
 eyepiece and monitor observation  in-vitro fertilization
 in reflected and transmitted light  examination of endocytosis and xenobiotics
 on upright and inverted microscopes  examination of phagocytosis
 in brightfield and phase contrast  injection of nucleotides
 in real color  examination of nerve cells
 in real time
 with extended depth of focus 3D microscopy is ideal for the following
 with improved resolution applications in materials microscopy:
 storage via recorder or image memory of the  defect analysis of semiconductor
PC components
 documentation via video prints or color  observation of surface topography in
photos microtechniques
 materials examinations in construction
engineering
The following microscopes can be upgraded
with the components for 3D microscopy:  rupture analysis

 Axiotron 2
 examination of the structure of microfibers

 Axiotech 100
 examination of the structure of crystalline
materials
 Axiotech 100 vario  examination of compound materials.
 Axiovert 100/135/135 M
 Axiovert 25 CA
 Axioplan 2/Axiophot 2
 Axioskop
 Axioskop 2

6 B 40-022-7 e 07/98
3D Microscopy Function  Eypiece version

2.2 Function
The dynamic technique on which 3D There are three different versions of 3D
microscopy is based can be used with any light microscopy:
microscope with an accessible aperture
diaphragm.
 eyepiece version
The technique can be used with upright or  monitor version
inverted reflected-light and transmitted-light  combination of eyepiece and monitor
microscopes. version
A patent has been applied for the technique.

2.2.1 Eyepiece version

An LCD shutter ("3D illumination shutter") is


positioned close to the aperture diaphragm and
is used to open or close one half of the beam
path.
With the eyepiece version, the LCD shutter is
controlled via the "3D-CONTROL DSA-3" unit.
The centroid of the illumination beam path is
switched in such a way that the object is
illuminated alternately from a right and a left
stereo angle.
The eyepiece shutters are controlled at the
same pulse rate, i.e. each eye only sees the
image on its own side.
right eye  right image
left eye  left image
A flicker-free display of the stereo image
composed of the right and left images is made
possible by an appropriately high pulse rate.
When the illuminator - eye allocation is
changed, a pseudostereo effect is created
1 Eyepiece with 3D-Shutter (height-changed image).
2 3D-CONTROL DSA-3
3 3D illuminator shutter
Resolution, contrast and depth can be
4 Objective optimized with relation to the object via the
5 Object plane aperture diaphragm.
Eyepiece version
in a reflected-light microscope

B 40-022-7 e 07/98 7
Function  Monitor version 3D Microscopy

2.2.2 Monitor version

An LCD shutter ("3D illumination shutter") is


positioned close to the aperture diaphragm and
is used to open or close half a side of the beam
path.
With the monitor version, the LCD shutter is
controlled via the "3MCU II" unit.
The centroid of the illumination beam path is
switched in such a way that the object is
illuminated alternately from a right and a left
stereo angle.
The camera and the monitor shutters are
controlled at the same pulse rate, i.e. each eye
only sees the image on its own side.
right eye  right image
left eye  left image
In the monitor version, allocation is made via
the display of the half images in two
perpendicular polarization directions which can
be allocated to the eyes by polarizing glasses.

1 Eyepiece with 3D-Shutter A flicker-free display of the stereo image


2 3D-CONTROL DSA-3 composed of the right and left images is made
3 Depolarizer possible by an appropriately high pulse rate.
4 3D illumination shutter
5 Condenser When the illuminator - eye allocation is
6 Object plane changed, a pseudostereo effect is created
7 Objective (height-changed images).
Eyepiece version
in a transmitted-light microscope Resolution, contrast and depth can be
optimized with relation to the object via the
aperture diaphragm.
The depolarizer is required for minimization of
disturbances by the polarization equipment and
facilitates work with birefringent objects

8 B 40-022-7 e 07/98
3D Microscopy Function  Monitor version

1 Monitor shutter 1 Monitor shutter


2 Glasses 2 Polarizing glasses
3 Control unit for monitor shutter 3 Camera
4 MCU II image memory 4 Objective
5 3D illumination shutter 5 Object plane
6 Objective 6 Condenser
7 Object plane 7 3D illumination shutter
8 Camera 8 MCU II image memory
9 Control unit for monitor shutter
Monitor version
in a reflected-light microscope Monitor version
in a transmitted-light microscope

B 40-022-7 e 07/98 9
Function  Combined version 3D Microscopy

2.2.3 Combination
of eyepiece and monitor version

An LCD shutter ("3D illumination shutter") is


positioned close to the aperture diaphragm and
is used to open or close one half of the beam
path.
With the combined version, the LCD shutter is
controlled via the "3D-CONTROL DSA-3" unit.
The centroid of the illumination beam path is
switched in such a way that the object is
illuminated alternately from a right and a left
stereo angle.
The eyepiece shutter and the camera and
monitor shutters are controlled at the same
pulse rate, i.e. each eye only sees the image
on its own side.
right eye  right image
left eye  left image
In the monitor version, allocation is made via
the display of the half images in two
perpendicular polarization directions which can
be allocated to the eyes by polarizing glasses.
1 Monitor shutter
2 Polarizing glasses A flicker-free display of the stereo image
3 Camera composed of the right and left images is made
4 Control unit for monitor shutter
5 MCU II image memory
possible by an appropriately high pulse rate.
6 3D-CONTROL DSA-3 When the illuminator - eye allocation is
7 3D illumination shutter
8 Depolarizer
changed, a pseudostereo effect is created
9 Object plane (height-changed image).
10 Eyepiece with 3D Shutter
Resolution, contrast and depth can be
Combined version optimized with relation to the object via the
in a transmitted-light microscope
aperture diaphragm.

10 B 40-022-7 e 07/98
3D Microscopy Maintenance and care

2.3 Maintenance and care Clean the polarizing foils as follows:

2.3.1 General
 Remove dust using a rubber blower
 Use a soft optics cleaning cloth
Never expose the components for 3D
microscopy to inadmissible climatic conditions
 Use a natural hair brush degreased in alcohol
and dried before.
(high humidity and temperature) for longer
periods of time. The glass plate of the window of the CCD
camera can be cleaned using the cleaning buds
The components must be protected from dust supplied.
and humidity and covered with the dust cover
after every use.

2.3.2 Cleaning of optical 2.3.3 Change of voltage and fuses


components
The 3D shutter in the eyepieces and in the 3D
illumination shutter are equipped with a
polarizing foil. Prior to connection to the line, check whether
the voltage set on the selector at the rear of the
3D-CONTROL DAS-3 unit complies with the line
voltage; it may be necessary to change from
230 V to 115 V.
The polarizing foils must not be cleaned with
solvents.
The foils are sensitive to scratching.

 Pull the power plug before changing the


voltage or the fuses
 Use a small screwdriver to turn back the
clamping piece of the fuse holder at the rear
of the 3D-CONTROL DAS-3 unit;
lift the fuse holder out of the fuse
department
 Remove the fuses
 Pull voltage selector out of its holder
 Turn voltage selector around 180° and insert
it back in its holder:
the new voltage is visible in the window
 Insert the fuses listed under "Technical Data"
1
2
Fuse compartment
Voltage selector  Insert fuse holder back in the fuse
3 Holding unit department until click-stop.
4 Window
Changing the voltage selector on the rear of the
3D-CONTROL DSA-3 unit

B 40-022-7 e 07/98 11
Troubleshooting 3D Microscopy

2.4 Troubleshooting
The monitor displays colored fringing or A pseudostereoscopic image is displayed,
several out-of-focus images: i.e. heights and depths in the image are
 Switch off MCU II;
exchanged:
switch it on again after 10 seconds  The toggle switch of the 3D CONTROL DAS-
3 is not set correctly;
No 3D image is obtained: set toggle switch to "STEREO"

 monitor shutter does not function;  For observation, exchange eyepieces for 3D
check switch for monitor shutter on the shutter
control unit; the green lamp lights up only if  For monitor observation,
the switches are set correctly set toggle switch of 3D CONTROL DAS-3 to
 parameters on the MCU II are not set "STEREO";
correctly; if required, change "Scope LCD" parameters
check the parameters, mainly Time, in the CONFIG menu of the MCU II
MEMORY IN, MEMORY OUT  For monitor observation,
 camera not positioned correctly; set the switch of the control unit for the
check whether the camera controls are monitor shutter to "stereo mode"
aligned perpendicular to the stand and
whether the monitor image follows the Note:
stage motion; If inverted microscopes are used in transmitted
if not, turn the camera light - e.g. the Axiovert 100/135/135 M -
 3D illumination shutter is out of alignment;
remember that observation is made from the
opposite side of the instrument if manipulators,
recenter aperture diaphragm,
needles and electrodes are used.
recenter 3D illumination shutter
 Knob of 3D-CONTROL DAS-3 control units
not set correctly;
check 3D setting of 3D-CONTROL DAS-3

The image flickers:


 pulse rate is not set correctly;
set 3D VIS on the 3D CONTROL DAS-3 for
observation, and 3D VIDEO for monitor
observation

12 B 40-022-7 e 07/98
3D Microscopy Technical Data

3 Technical Data

Parameters Description

Ambient conditions

Operation Storage Transport

Temperature + 10°C ... + 35°C 0°C ... + 35°C  20°C ... + 50°C

< 90% at 30°C < 90% to 30°C


Humidity max. 75% at 35°C condensation water not condensation water not
admissible admissible

Conditions of use Dust-free environment, absorption-free worktable

Safety

Protection degree I

Protection type IP 20

Excess voltage category II

Pollution degree 2

Radio interference suppression in accordance with EN 55011 (Class B)

Insensitivity to noise in accordance with EN 50082-1


The components for 3D microscopy meet the requirements of the EC directive
89/336/EEC and the EMC legislation of November 9, 1992

Power supply

3D-CONTROL DSA-3 230 VAC  10%, 50/60 Hz


switchable to 115 VAC  10%, 50/60 Hz

Power consumption of the overall


system
 30 VA; minimal 7 VA; maximal 200 VA (depending on configuration)

Fuses

Line input 115 VAC: T 0,2 A/250 V in accordance with IEC 127
3D-CONTROL DSA-3 230 VAC: T 0,1 A/250 V in accordance with IEC 127

For MCU II, monitor shutter, monitor and microscope, see the enclosed Technical Data sheets.

B 40-022-7 e 07/98 13
3D Microscopy
Axioskop

14 B 40-022-7 d 07/98
3D Microscopy
Axioskop Contents

3D Microscopy for Axioskop


Contents
1 Components for 3D Microscopy  Overview .............................................................. 16
2 Eyepiece version............................................................................................................ 18
2.1 Converting the microscope .............................................................................................. 18
2.2 3D-CONTROL DSA-3 control unit .................................................................................... 21
2.2.1 Connections..................................................................................................................... 21
2.2.2 Controls........................................................................................................................... 21
2.3 Adjustment ...................................................................................................................... 22
2.4 Check the contrast........................................................................................................... 23
2.5 Check whether image is upright ...................................................................................... 24
3 Monitor version............................................................................................................. 25
3.1 Conversion....................................................................................................................... 26
3.2 MCU II Control Unit ......................................................................................................... 28
3.2.1 Controls........................................................................................................................... 28
3.2.2 Basic settings for 3D microscopy ...................................................................................... 28
3.3 Adjustment ...................................................................................................................... 29
3.4 3D video recordings ......................................................................................................... 30
3.5 Printing of 3D video recordings ........................................................................................ 30
4 Eyepiece and monitor version .................................................................................... 31
4.1 Conversion and adjustment ............................................................................................. 31
4.2 Observation ..................................................................................................................... 32
4.2.1 Eyepiece observation........................................................................................................ 32
4.2.2 Monitor observation ........................................................................................................ 32

Illustrations
Fig. 1 Overview ......................................................................................................................... 16
Fig. 2 Attachment of 3D condenser adapter .............................................................................. 18
Fig. 3 Insertion of quartz depolarizer ......................................................................................... 19
Fig. 4 Insertion of phase stop 20................................................................................................ 19
Fig. 5 Connections for eyepiece version ..................................................................................... 20
Fig. 6 Changing the voltage selector on the rear of 3D-CONTROL DSA-3 .................................. 20
Fig. 7 3D-CONTROL DSA-3, front .............................................................................................. 21
Fig. 8 Insertion of 3D illumination shutter in the 3D condenser adapter ................................... 22
Fig. 9 Adjustment of 3D illumination shutter, pupil ................................................................... 22
Fig. 10 Labels to mark the eyepieces and plugs............................................................................ 24
Fig. 11 Wiring diagram for monitor version ................................................................................. 25
Fig. 12 Attachment of monitor shutter ........................................................................................ 26
Fig. 13 Rear of MCU II ................................................................................................................. 26
Fig. 14 Attachment of camera ..................................................................................................... 27
Fig. 15 MCU II controls, front ...................................................................................................... 28
Fig. 16 Wiring diagram for video prints....................................................................................... 30
Fig. 17 Wiring diagram for eyepiece and monitor version ............................................................ 31

B 40-022-7 e 07/98 15
3D Microscopy
Overview Axioskop

1 Components for 3D Microscopy  Overview

Fig. 1 Overview

16 B 40-022-7 e 07/98
3D Microscopy
Axioskop Overview

Axioskop

Eyepiece version Monitor version Eyepiece and monitor version

5 3D condenser adapter 3D condenser adapter 3D condenser adapter

4 3D illumination shutter 3D illumination shutter 3D illumination shutter

3 Eyepiece PL 10x/25 with 3D shutter Eyepiece PL 10x/25 with 3D shutter


and phase stop 20 and phase stop 20

2 Quartz depolarizer Quartz depolarizer

Centering telescope (optional) Centering telescope (optional) Centering telescope (optional)


Cat. No. 444830 Cat. No. 444830 Cat. No. 444830

1 3D-CONTROL DSA-3 3D-CONTROL DSA-3

7 TV adapter 60 C 2/3“ TV adapter 60 C 2/3“

8-13 3D video equipment with 3D video equipment with monitor


monitor shutter, including shutter, including camera, monitor
camera, monitor and polarizing and polarizing glasses
glasses

BNC cable

Key to Fig. 1
1 3D-CONTROL DSA-3
2 Quartz depolarizer
3 Eyepieces with 3D shutter and phase stop 20
4 3D illumination shutter
5 Condenser adapter
6 Condenser carrier
7 TV adapter
8 Camera
9 MCU II control unit
10 Control unit for monitor shutter
11 17“ monitor
12 Monitor shutter
13 Polarizing glasses

B 40-022-7 e 07/98 17
3D Microscopy
Eyepiece version Axioskop

2 Eyepiece version

2.1 Converting the microscope

 Set up the microscope in accordance with


the operating manual of the Axioskop
 Loosen condenser from the carrier and lift it:
the stage is in the uppermost position, the
condenser carrier in the lowest position
 Insert 3D condenser adapter in condenser
carrier and tighten the locking screw
Fig. 2

When inserting the adapter, make sure that
the recess for the 3D illumination shutter in
the 3D condenser adapter is not on the
same side as the stage drive, since they
obstruct each other.
The 3D condenser adapter can be screwed
to the carrier in two positions rotated
through 180°.
The orientation screw and the fixation screw in
the 3D condenser adapter may have to be
relocated.

 Insert condenser in 3D condenser adapter


and lock it in its position.
1 Microscope stage
2 Condenser
3 Stop screw
4 Fixation screw
5 Condenser carrier
6 Orientation pin (resilient)
7 Recess for 3D illumination shutter
8 Condenser stop
9 Condenser adapter
10 Orientation notch
Fig. 2 Attachment of 3D condenser adapter

18 B 40-022-7 e 07/98
3D Microscopy
Axioskop Eyepiece version

 Insert depolarizer:
Fig. 3
 Remove tube
 Insert depolarizer in the open aperture in
the stand and lock it in position
 Attach tube again

1 Quartz depolarizer
2 Locking screws
Fig. 3 Insertion of quartz depolarizer

 Insert phase stop 20 in eyepieces with 3D


shutter:
Fig. 4
 Remove lower part of eyepiece
 Insert phase stop
 Screw on lower eyepiece part again

1 Upper part of eyepiece


2 Phase stop 20
3 Lower part of eyepiece
Fig. 4 Insertion of phase stop 20

B 40-022-7 e 07/98 19
3D Microscopy
Eyepiece version Axioskop

 Connect components for 3D microscopy to


3D-CONTROL DSA-3:
Fig. 5
 Connect eyepieces with 3D shutter to the
EYEPIECE sockets
 Connect 3D illumination shutter to the
ILLUMINATION SHUTTER socket
 Insert eyepieces with 3D shutter in the
binocular tube

Connection to the line:

Prior to connection to the line, check whether


1 Eyepieces with 3D shutter
2 3D-CONTROL DSA-3 the voltage set on the rear of the
3 Line connection 3D-CONTROL DSA-3 unit complies with the line
4 3D illumination shutter voltage; it may be necessary to change from
Fig. 5 Connections for eyepiece version 230 V to 115 V
Fig. 6
 Pull the power plug before changing the
fuses or the voltage
 Use a small screwdriver to press out the
clamping piece of the fuse holder at the
rear of the 3D-CONTROL DSA-3 unit; lift
the fuse holder out of the fuse
compartment
 Remove the fuses
 Pull voltage selector out of its holder
 Turn voltage selector around 180° and
insert it back in its holder:
the new voltage is visible in the window
 Insert the fuses listed under Technical
1 Fuse compartment
Data
2 Voltage selector  Insert fuse holder back in the fuse
3 Holding unit compartment until click-stop
4 Window
Fig. 6 Changing the voltage selector on the rear of 3D-
CONTROL DSA-3

20 B 40-022-7 e 07/98
3D Microscopy
Axioskop Eyepiece version

2.2 3D-CONTROL DSA-3 2.2.2 Controls


control unit Fig. 7
STEP
If the rotary switch is in the "STEP" position,
this button can be used to change to statically
inclined illumination for the left and right beam
path.

STEREO/PSEUDO
Toggle switch which determines the phase
position between the shutters.
In the "STEREO" position, a stereoscopic
(upright) image is created, while the image in
the "PSEUDO" position is pseudostereoscopic
(vertically reversed).
Fig. 7 3D-CONTROL DSA-3, front
Rotary switch for the setting of different
modes:
STEP step mode
(inclined illumination from the
left or right)
2D brightness position if toggle
switch is in "STEREO“ position
3D VIS 3D image, eyepiece version
3D VIDEO 3D image, monitor version

E1, E2
Potentiometer for voltage adjustment of the
eyepiece shutter (factory-aligned);
E1 acts on the left EYEPIECE socket;
E2 acts on the right EYEPIECE socket
2.2.1 Connections I1, I2
Fig. 7 Potentiometer for voltage adjustment of the 3D
EYEPIECE illumination shutter (factory-aligned)
Connection sockets for eyepieces with 3D I1 acts on one side of the
shutter 3D illumination shutter,
I2 acts on the other side of the
ILLUMINATION SHUTTER 3D illumination shutter
Connection socket for 3D illumination shutter

TRIGGER
Trigger input socket for connection
of the MCU II

B 40-022-7 e 07/98 21
3D Microscopy
Eyepiece version Axioskop

2.3 Adjustment

 Use microscope slide as an object and focus


on it using the 10x objective;
after adjustment, check setting using the
objective of the highest magnification
 Insert 3D illumination shutter in the recess
provided in the 3D condenser adapter:
Fig. 8
 Move rotary switch of the DSA-3 to the
STEP position:
Fig. 7
 Remove eyepiece and replace it with
centering telescope (if available)
 View the pupil, focus on the aperture
diaphragm;
one pupil half will be bright, the other dark:
Fig. 9
 Move 3D illumination shutter to the side
until both pupil halves appear identical in
size;
1 Stop screw if required, reset the stop screw
2 Condenser adapter hold 3D illumination shutter in position with
3 Fixation screws depending on integration position the fixation screw
4 3D illumination shutter

Fig. 8 Insertion of 3D illumination shutter in the


3D condenser adapter

Fig. 9 Adjustment of 3D illumination shutter,


pupil

22 B 40-022-7 e 07/98
3D Microscopy
Axioskop Eyepiece version

2.4 Check the contrast


If colors or inhomogeneity are visible in the Recommended:
pupil, change the voltage settings for the 3D Eyepieces with 3D shutter outside the
illumination shutter and the 3D shutter of the microscope
eyepieces on the potentiometers of the 3D-
Control DAS-3 until optimum contrast and  Hold eyepieces directly in front of your eye
brightness are achieved. or reverse eyepieces and view them from
There are two ways of adjusting the voltage of the rear from some distance
the 3D illumination shutter:  View both eyepieces against a white
background in the brightness position and
3D illumination shutter in the microscope, reset E1 or E2 until both eyepieces display a
eyepieces with 3D shutter not in the colorless gray shade and equal brightness
microscope After conclusion of the voltage settings, the
eyepieces with 3D shutter are inserted again. It
 Use the centering telescope to view the pupil may happen that brightness differences then
 Set the rotary switch of the DSA-3 unit to occur again between right and left; in such a
2D: case
brightness position  slightly turn the eyepieces in the tube
 View the contrast; (  10°); make sure to set maximum and
if required, use screwdriver to reset I1 or I2 homogeneous brightness
until both halves of the pupil feature equal
brightness and are almost colorless.
3D illumination shutter outside the microscope
 View 3D illumination shutter against a white
background in the brightness position and
reset I1 or I2 until both halves display equal
brightness
There are two ways of adjusting the voltage of
the eyepiece shutter:
3D illumination shutter outside the microscope,
eyepieces with 3D shutter in the microscope

 Set the rotary switch of the 3D-CONTROL


DSA-3 unit to 2D:
brightness position
 View the contrast ;
if required, use screwdriver to reset E1 or E2
until both eyepieces feature equal brightness

B 40-022-7 e 07/98 23
3D Microscopy
Eyepiece version Axioskop

2.5 Check whether image is upright

 Set the rotary switch of the DSA-3 unit to


3D VIS
 Set the toggle switch of the DSA-3 unit to
STEREO
 View a familiar object;
if the image is not upright, exchange the
eyepieces

 Mark the eyepieces and the appropriate


plugs with the labels supplied:
Fig. 10

E1 and E2 for the left or right


connection plug
L and R for the left or right
eyepiece
Fig. 10 Labels to mark the eyepieces and plugs

To avoid the cables on the eyepieces with 3D


shutter to be damaged, attach them to the rear
and fix them to the stand using the supplied
cable clamps.

 Place the required object on the stage and


focus on it;
adjust aperture diaphragm until optimum 3D
contrast is achieved

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3D Microscopy
Axioskop Monitor version

3 Monitor version

The following components from KAPPA are  Control unit for monitor shutter with power
required for the monitor version: unit and connecting cable
 MCU II control unit  3D illumination shutter
 CF15/4 MC-S camera  Control and supply cable between
 CF15/4 MC connecting cable for MCU II
MCU II and 3D illumination shutter or
monitor shutter
 Line cable
 Polarizing glasses
 Monitor (100 Hz) with connecting cable and
attached monitor shutter

Fig. 11 Wiring diagram for monitor version

B 40-022-7 e 07/98 25
3D Microscopy
Monitor version Axioskop

3.1 Conversion  Set up and configure the microscope in


accordance with the operating instructions
of the Axioskop
 Attach the binocular phototube
Use Velcro tape to stick the bracket to the
monitor housing
 Insert monitor shutter in the bracket in such
a way that the connector sockets are
positioned on the bottom right

Before connecting the components for the


monitor version, make absolutely sure to note
the following:
1
2
Monitor
Bracket
 First read the enclosed manual from KAPPA
3 Monitor shutter entitled "Color TV Camera for Microscopy
and 3D images“;
Fig. 12 Attachment of monitor shutter
chapter 5, "Safety regulations" should be
observed in particular.
 Cable connections may only be made if the
various components are switched off.
 It is important to use only the connectors
listed in the operating instructions of the
camera system.
 Make sure that the cables are not bent or
laid out in too narrow a radius.

 Set up the video equipment with monitor


shutter and make the electrical connections:
Fig. 11/Fig. 13

1 Loop-through input for video printer/video recorder


2 Socket for camera cable 8 External triggering of the Freeze function
3 Socket for video printer/video recorder 9 Cold instrument socket/fuse holder
4 Output for monitor with Y/C input 10 INTERN: standard setting
5 Output for monitor with (F)BAS video input EXTERN: if video printer/video recorder
6 Socket for 3D-CONTROL DSA 3 trigger is connected
7 Socket for 3D illumination shutter 11 Output for monitor with RGB input
12 Connector for monitor shutter
Fig. 13 Rear of MCU II

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3D Microscopy
Axioskop Monitor version

 Switch phototube to camera/TV


(pushrod pulled out)
 Connect camera to stand via instrument-
specific video adapter or video zoom;
the following adapters are recommended:
 video zoom adapter 0.4x  2x
with C-mount
 TV adapter 1x for chip camera
with C-mount
Fig. 14

The type label on the back of the camera
must point to the rear;
if the image is reversed, turn the camera
around 180°;
rotation around 90° does not produce a
stereo effect.
1 Camera
Fig. 14
2 Adapter
3
4
Phototube
Pushrod  Insert 3D illumination shutter in the recess
provided in the 3D condenser adapter:
Fig. 14 Attachment of camera
Fig. 8

Do not touch the polarizing foil, since it can


be easily scratched.

 Switch components on in the following


order:
 microscope
 monitor
 MCU II control unit
Running-up time of the monitor shutter:
 3 minutes

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3D Microscopy
Monitor version Axioskop

3.2 MCU II Control Unit 3.2.2 Basic settings for 3D


microscopy

USER MENU User 1


RESET MENU Scope LC AB <BA>
(corresponds to stereo/
pseudostereo change)
White set Set Lock
R-B 50 %
Integration Mode: Auto
Time: 20 ms
Clock: Normal
Camera menu Enhance High
AGC On
AIT Off
1 Line switch Exposure Measure Integral
2 Menu change Level 50 %
3 Menu display (LCD)
4 Storage key for current image MCU control Gain: 80 %
5 Arrow keys for parameter control in the menu Offset: 50 %
Fig. 15 MCU II controls, front
Color: 50 %
Hue: 50 %
Red: 50 %
Blue: 50 %
Memory In Frame
Memory Out 100 Hz
Frame
3.2.1 Controls
Fig. 15

LCD-Display
Display of menu options
Control keys 
Arrow keys for options and settings within the
menus
Mode
Key for menu switching
Freeze
Key for storage of the current image;
the stored image is constantly made available at
the video ports

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3D Microscopy
Axioskop Monitor version

3.3 Adjustment
 Use microscope slide as an object and focus
on it using the 10x objective;
after adjustment, check setting using the
objective of the highest magnification
 Check center position of the 3D illumination
shutter in the same way as for the eyepiece
version
(page 22/Fig. 9);
for this, remove the camera cable from the
MCU II or from the camera:
(page 26/Fig. 13)
 Place the required object on the stage and
focus on it
 Switch beam path to camera/TV

 A two-dimensional image appears on the


monitor first;
if the image is reversed, turn the camera
around 180°
 Wear polarizing glasses:
the image appears in 3 dimensions on the
monitor
 Focus depending on the object;
adjust aperture diaphragm until optimum 3D
contrast is achieved

B 40-022-7 e 07/98 29
3D Microscopy
Monitor version Axioskop

3.4 3D video recordings Connecting the video recorder:


 Set the toggle switch of the MCU II to
3D sequences can generally be recorded and Extern
played back using a video recorder. Fig. 13
This requires an S-VHS recorder and an
S-VHS video tape.  Connect Y/C-In of the MCU II with Y/C-Out
of the video recorder
 Connect Y/C-Out of the MCU II with Y/C-In
of the video recorder
Recording, playback:
 Activate TBC (Time Base Corrector) on the
video recorder
 First activate the Pause/Still key, then the
Record key of the video recorder
 Use the Pause key to interrupt recording
 Interrupt playback using the Pause key and
end it using the Stop key.

3.5 Printing of 3D video


recordings
Stored 3D video recordings can be printed as
video prints:
Fig. 16
Storing the image:
 Set 100 Hz in the MEMORY OUT menu
of the MCU II
 Set Frame in the MEMORY IN menu of
the MCU II
 Store the current image via the Freeze key
Fig. 16 Wiring diagram for video prints Printing:
 Set 50 Hz in the MEMORY OUT menu of
the MCU II
 
Set Frame via the key;

use the control key to go to Field and
print the two half images - field 1 and
field 2 - one after the other.

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3D Microscopy
Axioskop Eyepiece and monitor version

4 Eyepiece and
monitor version
4.1 Conversion and adjustment
 Set up and configure the microscope in
accordance with the operating instructions
of the Axioskop
 Set up the eyepiece version
see page 18
Before connecting the components, first read  Set up the monitor version
the enclosed manual from KAPPA entitled see page 25
"Color TV camera for Microscopy and 3D
Images";  Connect eyepiece and monitor version in
chapter 5, "Safety regulations", should be accordance with the wiring diagram
observed in particular. Fig. 17

Fig. 17 Wiring diagram for eyepiece and monitor version

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3D Microscopy
Eyepiece and monitor version Axioskop

4.2 Observation 4.2.2 Monitor observation

This is a combination of the eyepiece and the


 Switch beam path to camera/TV on the
phototube
monitor version. The image is 3-dimensional (pushrod pulled out)
when you look through the eyepiece or on the
monitor.  Set rotary switch of 3D-CONTROL DSA-3
unit to the 3D VIDEO mode
 Place the required object on the stage and
focus on it;
4.2.1 Eyepiece observation the image on the monitor is 2-dimensional
 Set the beam path to observation on the
at first
phototube  If the image is reversed, turn the camera
(pushrod pushed in) around 180°
 Set the 3D VIS mode via the rotary switch  Wear polarizing glasses;
of the 3D-CONTROL DSA-3 control unit the image on the monitor is 3-dimensional
 Place the required object on the stage and  Use a familiar object to check whether the
focus on it; image is upright;
adjust aperture diaphragm until optimum if the image is not upright, switch to Scope
3D contrast is achieved; LCD in the RESET MENU of the MCU II
the image appears 3-dimensional through
the eyepiece  Focus depending on the object;
adjust aperture diaphragm until optimum
3D contrast is achieved

32 B 40-022-7 e 07/98