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SILABUS BIOMOLEKULER SERANGGA (ENT-712)

Prof. Dr. Inneke F. M. Rumengan, M.Sc.


Prof. Dr. Christina L. Salaki, M.S.
Prof. Dr. Trina E. Tallei, M.Si.

Deskripsi Mata Kuliah


Sasaran MK ini yaitu untuk memperkenalkan konsep dan metode baru dalam biologi molekuler
serangga yang meliputi genetika molekuler serangga, sistematika dan molekuler serangga, dan
manipulasi genetik secara umum (termasuk CRIPSPR). Mata Kuliah ini dirancang untuk memudahkan
mahasiswa dalam pembelajaran dan turut serta berpartisipasi secara aktif. Journal reading
diwajibkan untuk mendapatkan informasi dan memahami penelitian terkini dalam bidang
biomolekuler serangga.

Waktu pelaksanaan : Kamis 10.00-12.00

Tanggal Topik Deskripsi


1 Feb Introduction to DNA, Pengenalan DNA dan RNA
IFMR RNA, genes and genome
8 Feb Introduction to DNA, Pengenalan gen dan genom
IFMR RNA, genes and genome
15 Feb Genomik, proteomik,  Short Views on Insect Genomics and Proteomics
IFMR dan metabolomik  Entometabolomics: applications of modern analytical
serangga techniques to insect studies
22 Feb Transgenics insect  Insect transformation for both experimentation and
IFMR population control.
 Transformasi menggunakan transposable element
1 Maret DNA metabarcoding of  Environmental metabarcodes for insects:
IFMR insects  Metabarcoding as a tool for investigating insect diversity
 The utility of DNA metabarcoding for studying the response
of arthropod diversity and composition to land-use change
in the tropics
8 Maret Next generation  Application of next-generation sequencing to the study of
TET sequencing non-model insects.
 using next-generation sequencing to enhance and
accelerate DNA barcode capture from single specimens
15 Molecular systematics  Why study Molecular Systematics? New kinds of molecular
Maret data
TET  Tree of Life, the future of systematics, new controversies in
Evo-Devo, barcoding pros and cons.
22 Molecular phylogenetic  phylogenetic reconstruction and tree-thinking in recent
Maret research.
TET  Using biological data bases and inferring phylogenies
(programs for students to download and use).
 Insect phylogenomic
29 Insect RNAi (RNA  Recent advances in RNA interference research in insects:
Maret interference) Implications for future insect pest management strategies
TET  RNAi Efficiency, Systemic Properties, and Novel Delivery
Methods for Pest Insect Control
 RNAi for studying gene/protein function
5 April CRISPR – cas9 in insect  Applications, best practices and biosafety concerns

1
TET  CRISPR-Cas9 knockouts
 Advances and perspectives in the application of
CRISPR/Cas9 in insects
 Progress and Prospects of CRISPR/Cas Systems in Insects
12 April Endophytic  Role of endophytes in Insect control
TET microorganism in  Drug discovery
insects (bacteria and
fungi)
19 April Defense mechanisms of
CSL insects and its
molecular biology
26 April Gene structure ofPost-translation modification in insects using baculovirus
CSL baculovirus and vectors: secretion, amidation, glycosylation, internal cleavages
construction ofof polypeptides
expression vectors Insect toxins: gene structure and use for insect control using
the baculovirus vector
Biological control using insect viruses
3 Mei Insect molecular ecological processes based on the analysis of
CSL ecology biomacromolecules, particularly DNA, RNA, and proteins, but
also of low-molecular weight signal compounds.
10 Mei Molecular Biology of
CSL Insect Disease Vectors
17 Mei Molecular detection of The Challenges and Advances in Diagnosis of Vector-Borne
CSL insect-borne patogens Diseases .

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