Dr. Ahmad Kaleem Qureshi & Dr.

Naveed Sajid

2/25/2018 Qualitative
Organic Analysis
Identification Methods

Dr. Ahmad Kaleem Qureshi & Dr. Naveed Sajid

IUB
Dr. Ahmad Kaleem Qureshi & Dr. Naveed Sajid
Experiment No.

Statement: Identify the given organic compound

Experiment Observations Inference

Physical state
Color
Odor
Flame test
Melting point
Br2 Water test
O.C + CCl4 + Br2 soln. in CCl4+ shake
KMnO4 Test
O.C + Alkaline KMnO4 soln.+ shake
Le Rosen test
O.C + 1ml of Formaldehyde +few drops of
H2SO4.
Nitration Test
O.C + 2ml HNO3 + 2 mL Conc. H2SO4 + heat for
5 minute
Lassaigne's(Sodium Fusion) Test
FOR NITROGEN

Na extract 2 mL + NaOH soln. + FeSO4 + boil

and cool + 1 mL dil. H2SO4
FOR SULPHUR

Na extract2 mL + Na- nitroprusside soln.

FOR NITROGEN&SULPHUR

Na extract 2 mL + 1-2 drops of HCl + 1-2 drops

of FeCl3
FOR HALOGENS

Na extract 2 mL + dil. HNO3 + boil and cool +

AgNO3
Solubility Tests
O.C + distilled water
O.C + distilled water + litmus paper

O.C + NaHCO3 soln.

O.C + dil. NaOH
O.C + dil. HCl
Dr. Ahmad Kaleem Qureshi & Dr. Naveed Sajid
O.C + H2SO4
Functional Group Test
Chromic acid Test
O.C + 1 mL acetone + 1 drop of the chromic acid
+ Shake

Lucas’s Test
O.C + 1 mL HCl + 1 mL ZnCl2+ Shake
vigorously, then allow the mixture to stand.

2,4-Dinitrophenylhydrazine Test
O.C + 2 mL of 95% ethanol + 3 mL of 2,4-
dinitrophenylhydrazine reagent + Shake
If no precipitate appears within 5 minutes, heat
the solution in a hot water bath for 5 minutes and
then allow it to cool to room temperature.

Benedict’s Test
O.C + 2 mL of water +2 mL of Fehling’s soln. +
Heat the mixture to boil.
(copper(II) sulfate, sodium citrate, and sodium
carbonate)
Fehling's Test
O.C + 2 mL of water +2 mL of Fehling’s reagent+
Heat the mixture to boil.
Tollen’s (silver mirror) Test
O.C + 1 mL Tollen’s reagent+ Gently heat
Hinsberg’s Test
50 mg O.C + 0.2 g of benzene sulfonyl chloride +
4 mL of 10% aqueous NaOH + Shakevigorously
Ferric chloride Test
O.C soln. + 1% FeCl3 soln.+ Shake
Molisch’s Test
O.C + 2 mL of water + 2-3 drops of beta-
naphthol soln. + Very gently add 1ml of Conc.
H2SO4along the side of the test tube.

LITERATURE SURVEY
Dr. Ahmad Kaleem Qureshi & Dr. Naveed Sajid

CONFIRMATORY TEST

DERIVATIVES

RESULT