CELLENISTM

Platelet-Rich Plasma
Preparation System in
Medical Aesthetics
 Platelet-Rich Plasma (PRP)

• A progressive non-surgical healing treatment in a variety of fields,

including dermal aesthetics, sports medicine, orthopedics, dentistry,
wound healing etc.

• A fraction of autologous blood with supraphysiological concentration

of platelets and their associated growth factors.

• Upon activation, platelet-derived α granules release autologous

growth factors involved in cell proliferation, angiogenesis, collagenesis
and matrix remodelling (PDGF/ TGF/ VEGF/ EGF/ FGF/ IGF etc).

• PRP is thought to promote physiological wound healing and rapid

tissue regeneration.
The Wound Healing Cascade

PLT-Haemostasis
Wound Healing
 Growth Factors in PRP
GROWTH FACTOR
Platelet –derived GF (PDGF)
Transforming GF β group (TGF)
Vascular Endothelial GF (VEGF)
Epidermal GF (EGF)
Fibroblast GF (FGF)
FUNCTION
Stimulates cell proliferation
Promotes angiogenesis
Promotes epithelialization
Promotes granulation tissue formation
Stimulates collagen and matrix production
Promotes formation of extracellular matrix
Regulates bone cell metabolism by inhibiting
osteoclasts and promoting osteoblasts proliferation
Promotes angiogenesis
Promotes cell differentiation and stimulates
reepithelialisation, angiogenesis
Promotes proliferation of endothelial cells and
fibroblasts
Stimulates angiogenesis
 PRP – Clinical Applications

Sports Medicine
Orthopaedics

Aesthetic
Dermatology PRP Wound Care
“Vampire face lift”

Dental Surgery
Hair Loss
 PRP in Aesthetics

• Rejuvenation and delay of the aging process by stimulating:

• Fibroblast proliferation
• Stem cell proliferation and differentiation
• Collagenesis and ECM formation
• Vascularization

• PRP Benefits:
• Improved skin texture and tone (silkiness)
• Refinement of signs of aging (lines, spots)
• Reduction of dark circles
• Hydration
• Reduction of scars (acne, stretch marks)
• Gradual increase in skin thickness and vitality
 PRP Applications in Aesthetics

• Sun-damaged and aged skin

• Cheeks and mid-face
• Nasolabial folds
• Around the eyes
• Jaw Line
• Sagging skin on the neck
• Back of the hands
• Décolletage
• Scars
• Other body areas
PRP injection Techniques:
• Mesotherapy (intradermis)
• Dermaroller (micro-needling)
• Mixed with fillers, such as HA
PRP – Summary of Unique Benefits
in Medical Aesthetics

• Preserves natural appearance

• Can be used for areas not suitable for other treatments,
such as around the eyes
• May be combined with other treatments to stimulate
synergistic biological effect
• Noticeable effect within ~2-3 weeks, effect visible up to 1-
1.5 years (with maintenance)
- Preparation Procedure
- Preparation Procedure (2)
 - Unique Features

Optimal Biological Profile of PRP due to the Separation Gel :

• High Platelet Concentration (X4-5 fold), ~90% PLT yield

• Reduction of Granulocytes, which may mediate catabolic effect (MMPs)

• Eliminating RBC, which may inhibit proliferation and induce apoptosis (1)

• Mononuclear cells induce anabolic effect , increase collagen expression

and assist fighting infection (2).

1. Blood Cells Inhibit Proliferation and Stimulate Apoptosis in Human Lung Fibroblasts In Vitro. Scand J Immunol. 2004
2. Peripheral Blood Mononuclear Cells Enhance the Anabolic Effects of Platelet-Rich Plasma on Anterior Cruciate Ligament Fibroblasts. J Orthop Res. 2013
 - PRP vs. Whole Blood (WB)

WB

PRP
~90% PLT yield
- Performance Evaluation (1)

* “Mycells” , “Tropocells” and “Cellenis “ are commercial

names of Estar’s PRP Systems.

Platelet and growth factor concentrations in activated

platelet-rich plasma: a comparison of seven commercial
separation systems Kushida S. et al J Artif Organs 2014
 - Performance Evaluation (2)

* “Mycells”, “Tropocells” and “Cellenis” are commercial names of Estar’s PRP Systems.

Platelet and growth factor concentrations in activated platelet-rich plasma: a comparison of seven commercial
separation systems Kushida S. et al J Artif Organs 2014
 - Literature Review

• Clinical Studies with CellenisTM / TropocellsTM have been

published in the following clinical fields:

• Aesthetic Dermatology
• Hair Loss
• Plastic Surgery (Striae Distensae)
• Orthopaedics (Jumper’s knee)
• Wound Healing
• Basic Science
 - in Aesthetics (1)

November/December 2011 | prime-journal.com

Amgar et al, 2011 – Study Outline

Study objective
To evaluate the efficacy of PRP facial skin treatment through objective parameters, such as
hydration rate and transepidermal water loss.
Design
37 female patients were treated with 4 cc of PRP at the lower part of the face. Control
group consisted of 2 patients, which received saline.
Biometric Parameters tested
Skin hydration, anisotropy, TEWL, Cutaneous micro-relief, Pigmentation, luminance,
temperature
Follow up:
Three follow up visits at 21-28d, 90d and 120d.
Outcome:
3 weeks post treatment a good anti-ageing effect was evidenced (24% improvement),
which increased with time (33%) . The positive effect of one treatment lasted up to ten
months.
Amgar et al, 2011 - Clinical Aspect of Treatment

Zones of injection and (A) Before (B) 1 month after (C) 3 months later
measurement

120
in Aesthetics (2)
 for Hair Growth

Before and 12 months after

PRP Treatment

Before and 9 months after

PRP Treatment

Methods
The scalp was first traumatized with a 1.5 mm microneedling roller, followed by two 10 ml PRP
injections at 3 months intervals
Results
At 3 months an increase in hair density of 18.8% was observed, as well as a decrease in the
average of caliber (-2%), as the new hairs are thin. At 9 months the density increased in 29% and
the caliber in 5%.
 for Striae Distensae

Int J Dermatol. 2012, Kim et al.

Methods
19 patients. 3 sessions of intradermal RF combined with PRP at intervals of 4 weeks
Outcome
5.3% achieved excellent improvement; 36.8% - marked improvement; 31.6% - moderate
improvement and 26.3% - mild improvement.
 in Orthopaedics

Methods: RCT L1. 46 athletes with jumper’s randomized into 2 groups: 2 PRP
injections (over 2 weeks under US (PRP group), and 3 sessions of focused extracor-poreal shock
wave therapy (ESWT group).

Results: The PRP group showed significantly better improvement than the
ESWT group in VISA-P, VAS scores at 6- and 12-m follow-up, and modified
Blazina scale score at 12-m follow-up.

Conclusions: Therapeutic injections of PRP lead to better midterm clinical

results compared with focused ESWT in the treatment of jumper’s knee.
 in Wound Care

Methods : Activated PRP was applied to the wound

as a liquid or gel form according to wound size,
location and condition. Prior to the application,
necrotic tissues were debrided using a scalpel.

Conclusions: Topical application of PRP to acute

and chronic skin ulcers significantly accelerated the
epithelization process, likely through upregulation of
the cell cycle regulatory proteins cyclin A and CDK4.
 in Basic Science of Fibroblasts

Methods:
PRP-treated human skin fibroblasts in vitro.

Outcome:
Increase in expression of G1 cell cycle regulators and type I collagen, thereby accelerating the wound healing
process.
 Unique Features - Summary

• Easy to use, safe, rapid and effective PRP system

• Optimal platelet concentration and biological profile

• Established clinical evidence

• Physiological pH – minimizes patient discomfort

• Filter sleeve to facilitate efficient PRP harvest

• CE certified Medical Device, FDA 510(k) cleared

• Manufacture according to EN ISO 13485 and ISO 9001 standards

 Thank You!

Business Development yuval@estar-medical.com

R&D yael@estar-medical.com

www.estar-medical.com
Tel: +972 3-5596414| Fax: +972 3-5596424