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This sequencing report provides information about possible issues that could cause sequencing results and suggestions to address them. The sequencing result could show no signal, a signal disorder, or signal interruption. For a no signal result, possible issues include insufficient DNA concentration, primer problems, or cloning failures. For a signal disorder, issues may be low primer specificity, multiple templates, or sample quality problems. Signal interruption could arise from homopolymers, repeats, or secondary structures. Suggestions are provided such as redesigning primers, increasing DNA concentration, or using structure-releasing reagents.
This sequencing report provides information about possible issues that could cause sequencing results and suggestions to address them. The sequencing result could show no signal, a signal disorder, or signal interruption. For a no signal result, possible issues include insufficient DNA concentration, primer problems, or cloning failures. For a signal disorder, issues may be low primer specificity, multiple templates, or sample quality problems. Signal interruption could arise from homopolymers, repeats, or secondary structures. Suggestions are provided such as redesigning primers, increasing DNA concentration, or using structure-releasing reagents.
This sequencing report provides information about possible issues that could cause sequencing results and suggestions to address them. The sequencing result could show no signal, a signal disorder, or signal interruption. For a no signal result, possible issues include insufficient DNA concentration, primer problems, or cloning failures. For a signal disorder, issues may be low primer specificity, multiple templates, or sample quality problems. Signal interruption could arise from homopolymers, repeats, or secondary structures. Suggestions are provided such as redesigning primers, increasing DNA concentration, or using structure-releasing reagents.
Sample name Sequencing result Possible reason Suggestion 2016207_ITS_ITS_F 2 2-6 Handler: Tiffany Chen Ext: 137
Sequencing Possible problem Suggestion
result (1-1) Insufficient DNA concentration. (1-1a) Please increase DNA concentration. (1-2)Wrong label of primer (1-2a) Please check primer concentration. concentration. (1) No (1-3) No priming sites (1-3a) Please check the accuracy of the primer. signal (1-4a) Please check the construction was successful or not. (1-4) A failure cloning. (1-4b) Please check the vector has been modified or not. (2-1a)Please re-design a new primer (Tm = 50℃ to (2-1) Low specificity of primer 55℃). (2-2) Insufficient DNA concentration (2-2a) Please increase DNA concentration. (2-3) Primer degradation (N-1, N-2..) (2-3a)Please check the storage condition of primer, or using a fresh dissolved primer instead (2-4a) No single product, please purify the products (2-4) Multiple templates again. (2) Signal (2-4b) Please choose a single colony. disorder (2-5a) Please re-design a primer having only one (2-5) Multiple priming sites binding site. (2-6) Fragment shift mutation (SNP, insertion or deletion). (2-7a) Please remove inhibitors completely among (2-7) Low quality of sample. sequencing reaction (ex: salt, phenol, EDTA, etc.) (2-8a) Please re-prepare the samples and check it (2-8) Sample smear on the gel (3-1a) Please use a new primer from another side. (3-1) Homopoly (T),(A),(G),(C) (3-1b)Please design a new primer to avoid this (3) Signal effect. interrup t (3-2) Secondary structure (3-2a) Please use a new primer from another side. (Repeat sequence, hair pin, (3-2b) Please use structure-releasing reagents. siRNA..) (dGTP, DMSO)
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