Standard Laboratory Equipment APPENDIX 2C

Special equipment is itemized in the materials list of each protocol. Listed below are
standard pieces of equipment in the modern food science laboratory—i.e., items used
extensively in this manual and thus not usually included in the individual materials lists.
See SUPPLIERS APPENDIX for contact information for commercial vendors of laboratory
equipment.
Applicators, cotton-tipped and wooden Ice buckets
Autoclave Ice maker
Balances, analytical and preparative Immersion oil for microscopy
Beakers Kimwipes, or equivalent lint-free tissues
Biohazard disposal containers and bags Lab coats
Blender (e.g., Waring Blendor) Laboratory glass ware
Bottles, glass and plastic Light box, for viewing gels and autoradiograms
Bunsen burners Liquid nitrogen and Dewar flask
Centrifuges, low-speed (6,000 rpm) and high- Magnetic stirrers (with heater is useful)
speed (20,000 rpm) refrigerated centrifuges, Markers, including indelible markers and
ultracentrifuge (20,000 to 80,000 rpm), and china-marking pencils
microcentrifuge that holds standard 0.5- and Microcentrifuge, Eppendorf-type, maximum
1.5-ml microcentrifuge tubes speed 12,000 to 14,000 rpm
NOTE: Centrifuge speeds are provided as g or Microcentrifuge tubes, 1.5-ml and 0.5-ml
as rpm (with example rotor models) Microscope, standard optical model
throughout the manual. (optionally with epifluorescence or
Cold room (4°C) or cold box phase-contrast illumination)
Computer (PC or Macintosh) and printer Microscope slides and coverslips
Conical centrifuge tubes, 15- and 25-ml plastic Microwave oven, to melt agar and agarose
Cuvettes, plastic disposable, glass, and quartz Mortar and pestle
Darkroom and developing tank, or X-Omat Muffle furnace
automatic X-ray film developer (Kodak) Ovens, drying, vacuum, and microwave
Desiccators (including vacuum desiccators) Paper cutter, large size, for 46 × 57-cm
and desiccant Whatman paper sheets
Dry ice Paper towels
Filtration apparatus, for collecting acid Parafilm
precipitates on nitrocellulose filters or Pasteur pipets and bulbs
membranes pH meter and pH standard solutions
Flasks, glass (e.g., Erlenmeyer, beveled pH paper
shaker) Pipet bulbs, or battery-operated pipetting
Forceps devices—e.g., Pipet-Aid (Drummond
Freezers, −20° and −80°C Scientific)
Gel electrophoresis equipment, horizontal Pipets, Pasteur and graduated, glass and plastic,
full-size and minigel apparatus, vertical serological (1- to 25-ml)
full-size and minigel apparatus for Pipettors, adjustable delivery, volume ranges
polyacrylamide protein gels, and specialized 0.5 to 10 µl, 10 to 200 µl, and 200 to
equipment for two-dimensional protein gels 1000 µl
Grinder (e.g., coffee grinder) Plastic wrap, UV transparent (e.g.,
Heat-sealable plastic bags and apparatus Saran Wrap)
Heating blocks, thermostat-controlled metal Polaroid camera
heating block that holds test tubes and/or Power supplies, 300-V for polyacrylamide
microcentrifuge tubes gels; 2000- to 3000-V for some applications
Hoods, chemical and microbiological Racks, for test tubes and microcentrifuge tubes
Hot plates, with or without magnetic stirrer Radiation shield, Lucite or Plexiglas
Gloves, plastic and latex, disposable and Radioactive waste containers, for liquid and
asbestos solid waste Laboratory Stock
Graduated cylinders Razor blades Solutions,
Equipment, and
Guidelines
Current Protocols in Food Analytical Chemistry (2001) A.2C.1-A.2C.2 A.2C.1
Copyright © 2001 by John Wiley & Sons, Inc.
Supplement 2
 Refrigerator, 4°C
Ring stands and rings
Rotator, end-over-end
Rubber bands
Rubber policemen
Rubber stoppers
Safety glasses
Scalpels and blades
Scintillation counter
Scissors
Shakers, orbital and platform
Spectrophotometer, UV and visible
Speedvac evaporator (Savant)
Stir-bars, assorted sizes
Tape, masking and electrician’s
Thermometers
Timer
UV transilluminator
Vacuum aspirator
Vacuum line
Volumetric flasks
Vortex mixers
Wash bottles, plastic and glass
Water baths, variable temperature up to 80°C
Water purification equipment, e.g., Milli-Q
system (Millipore) or equivalent
X-ray film cassettes and intensifying screens

Standard
Laboratory
Equipment

A.2C.2
Supplement 2 Current Protocols in Food Analytical Chemistry