Name:

Exercise 1: Cell Transport Mechanisms and Permeability: Activity 5: Simulating Active Transport Lab Report

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1. The movement of sodium and potassium maintained by the Na+ -K+ pump
You correctly answered: e. requires energy and is against a concentration gradient.

2. The sodium-potassium pump is classified as a(n)

You correctly answered: a. antiporter.

3. The sodium-potassium pump moves _____ sodium ions and _____ potassium ions simultaneously.
You correctly answered: b. 3, 2

4. Solutes that require active transport for movement might be too large to pass or might be
You correctly answered: a. lipid insoluble.

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Experiment Results
Predict Question:
Predict Question 1: What do you think will result from these experimental conditions?
Your answer : c. No Na+ will be transported.

Predict Question 2: Do you think the addition of glucose carriers will affect the transport of sodium or potassium?
Your answer : d. No, it will not affect the transport of either ion.

Stop & Think Questions:

Why did the sodium transport stop before the transport was completed?
Your answer : b. The potassium was depleted.
Correct answer: c. The ATP was depleted.

Why was the equilibrium for the solutes reached earlier?

You correctly answered: c. There were more pumps for transport.

Experiment Data:

Run Number Solute ATP Start Conc. L Start Conc. Pumps Carriers Rate
R
1 Na+ Cl- 1 9.00 0.00 500 --- 0.0188
1 K+ Cl- 1 0.00 6.00 500 --- 0.0125
1 Glucose --- 0.00 0.00 --- 0 0.0000
2 Na+ Cl- 3 9.00 0.00 500 --- 0.0025
2 K+ Cl- 3 0.00 6.00 500 --- 0.0017
2 Glucose --- 0.00 0.00 --- 0 0.0000
4 Na+ Cl- 0 9.00 0.00 500 --- 0.0000
4 K+ Cl- 0 0.00 0.00 500 --- 0.0000
4 Glucose --- 0.00 0.00 --- 0 0.0000
5 Na+ Cl- 3 9.00 0.00 800 --- 0.0042
5 K+ Cl- 3 0.00 6.00 800 --- 0.0028
5 Glucose --- 0.00 0.00 --- 0 0.0000
6 Na+ Cl- 3 9.00 0.00 800 --- 0.0042
6 K+ Cl- 3 0.00 6.00 800 --- 0.0028
6 Glucose --- 0.00 10.00 --- 400 0.0028#

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Post-lab Quiz Results
You scored 25% by answering 1 out of 4 questions correctly.

1. What happened when you increased the amount of ATP dispensed with the same concentration of sodium and
potassium on either side of the membrane?
Your answer: d. Fewer ions were transported.
Correct answer: b. More ions were transported.

2. At what concentration of ATP were the sodium and potassium maximally transported?
Your answer: b. 1 mM ATP
Correct answer: d. 3 mM ATP

3. What was the effect of adding more Na+ -K+ pumps to the simulated cell?
Your answer: d. The ions move with their concentration gradient.
Correct answer: a. Transport of the ions was faster.

4. Describe the effect of adding glucose carriers to the sodium and potassium transport.
You correctly answered: c. There was no change in the transport rate because glucose is transported independently.

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Review Sheet Results
1. Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM potassium chloride outside the cell,
instead of other concentration ratios.
Your answer:
Debido a que la bomba funciona en una proporción de 3:2( iones de sodio : potasio), la relación usada es justificada.

2. Explain why there was no sodium transport even though ATP was present. How well did the results compare with your
prediction?
Your answer:
Para la funcionalidad de la bomba, tiene que existir presencia de sodio y de potasio, la bomba no funciona.Debido a que la
bomba funciona en una proporción de 3: 2 iones de sodio / potasio, es comprensible la relación de 9mM de cloruro de
sodio y 6mM de potasio.

3. Explain why the addition of glucose carriers had no effect on sodium or potassium transport. How well did the results
compare with your prediction?
Your answer:
Para la funcionalidad de la bomba debe de haber tanto sodio como potasio, sin alguno de ellos no existe manera de que se
active.

4. Do you think glucose is being actively transported or transported by facilitated diffusion in this experiment? Explain your
answer.
Your answer:
Se transporta por difusión facilitada porque no había presencia de ATP, por lo que podemos suponer que el transporte fue
asistido por los carriers

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